The Cryo-EM Structure of Human Catalytic Step I Spliceosome (C complex) at 4.1 angstrom resolution
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 5yzg
Q-score: 0.205
Zhan X, Yan C, Zhang X, Lei J, Shi Y
Science (2018) 359 pp. 537-545 [ DOI: doi:10.1126/science.aar6401 Pubmed: 29301961 ]
- U2 snrna (60 kDa, RNA from Homo sapiens)
- Inositol hexakisphosphate (660 Da, Ligand)
- Rna-binding protein 8a (19 kDa, Protein from Human)
- Snw domain-containing protein 1 (61 kDa, Protein from Human)
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from Human)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Human)
- Crooked neck-like protein 1 (100 kDa, Protein from Human)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Human)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Pre-mrna-processing factor 17 (65 kDa, Protein from Human)
- Coiled-coil domain-containing protein 94 (37 kDa, Protein from Human)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Human)
- Small nuclear ribonucleoprotein-associated proteins b and b' (23 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Human)
- Pre-mrna-splicing factor cwc25 homolog (49 kDa, Protein from Human)
- Pleiotropic regulator 1 (57 kDa, Protein from Human)
- Intron-binding protein aquarius (171 kDa, Protein from Human)
- Peptidylprolyl isomerase domain and wd repeat-containing protein 1 (73 kDa, Protein from Human)
- Pre-mrna-splicing factor atp-dependent rna helicase prp16 (140 kDa, Protein from Human)
- Zinc ion (65 Da, Ligand)
- Unknow (2 kDa, Protein from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Peptidyl-prolyl cis-trans isomerase e (33 kDa, Protein from Human)
- Cell division cycle 5-like protein (92 kDa, Protein from Human)
- Pre-mrna-processing factor 19 (55 kDa, Protein from Human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Human)
- Protein casc3 (76 kDa, Protein from Human)
- Pre-mrna-splicing factor isy1 homolog (35 kDa, Protein from Human)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from Human)
- Protein bud31 homolog (17 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Human)
- Pre-mrna (88 kDa, RNA from Human)
- Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from Human)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Human)
- U6 snrna (34 kDa, RNA from Homo sapiens)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Human)
- Magnesium ion (24 Da, Ligand)
- Pre-mrna-splicing factor spf27 (26 kDa, Protein from Human)
- Eukaryotic initiation factor 4a-iii (46 kDa, Protein from Human)
- Peptidyl-prolyl cis-trans isomerase g (88 kDa, Protein from Human)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from Human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Human)
- Pre-mrna-splicing factor syf2 (28 kDa, Protein from Human)
- U5 snrna (37 kDa, RNA from Homo sapiens)
- Protein mago nashi homolog 2 (17 kDa, Protein from Human)
- Human catalytic step i spliceosome (Complex from Homo sapiens)