Electron Microscopy Data Bank

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High-resolution cryo-EM structure of Pyrococcus abyssi 30S ribosomal subunit bound to mRNA and initiator tRNA anticodon stem-loop

Release date: June 29, 2022
Resolution: 2.25 Å
EM Method: Single-particle
Fitted PDBs: 7zhg
Q-score: 0.696

Kazan R, Bourgeois G, Lazennec-Schurdevin C, Larquet E, Mechulam Y, Coureux PD, Schmitt E

Nucleic Acids Res (2022) 50 pp. 6532-6548 [ Pubmed: 35694843 DOI: doi:10.1093/nar/gkac490 ]

Sample:

30s ribosomal protein s28e (8 kDa, Protein from Pyrococcus abyssi GE5)
Rrna 16s (487 kDa, RNA from Pyrococcus abyssi GE5)
30s ribosomal protein s10 (11 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s14 type z (6 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s15 (18 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s7 (24 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s4e (28 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s17e (8 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s9 (15 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s6e (14 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s12 (16 kDa, Protein from Pyrococcus abyssi GE5)
Mrna (5 kDa, RNA from Pyrococcus abyssi GE5)
Magnesium ion (24 Da, Ligand)
30s ribosomal protein s17 (13 kDa, Protein from Pyrococcus abyssi GE5)
High resolution 30s ribosomal subunit with a fragment of mrna and trna (Complex)
30s ribosomal protein s27ae (5 kDa, Protein from Pyrococcus abyssi GE5)
High resolution 30s ribosomal subunit with a fragment of mrna (Complex from Pyrococcus abyssi GE5)
Met-trnaimet (24 kDa, RNA from Pyrococcus abyssi GE5)
30s ribosomal protein s19e (17 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s11 (14 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s5 (26 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s13 (16 kDa, Protein from Pyrococcus abyssi GE5)
50s ribosomal protein l7ae (13 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s19 (15 kDa, Protein from Pyrococcus abyssi GE5)
Zinc ion (65 Da, Ligand)
30s ribosomal protein s8 (14 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s2 (23 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s27e (7 kDa, Protein from Pyrococcus abyssi GE5)
50s ribosomal protein l41e (4 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s8e (14 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s24e (11 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s3 (23 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s3ae (22 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s4 (21 kDa, Protein from Pyrococcus abyssi GE5)
Water (18 Da, Ligand)
Met-trnaimet (Complex from Pyrococcus abyssi GE5)
Zn-ribbon rna-binding protein involved in translation (7 kDa, Protein from Pyrococcus abyssi GE5)

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