Complex I from E. coli, DDM/LMNG-purified, under Turnover at pH 6, Open state
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 7p64
Q-score: 0.666
Kravchuk V, Petrova O, Kampjut D, Wojciechowska-Bason A, Breese Z, Sazanov L
Nature (2022) 609 pp. 808-814 [ Pubmed: 36104567 DOI: doi:10.1038/s41586-022-05199-7 ]
- Nadh-quinone oxidoreductase (99 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit k (10 kDa, Protein from Escherichia coli BL21(DE3))
- Eicosane (282 Da, Ligand)
- Nadh-quinone oxidoreductase subunit f (48 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh dehydrogenase i subunit m (56 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit b (25 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit a (16 kDa, Protein from Escherichia coli BL21(DE3))
- Calcium ion (40 Da, Ligand)
- Nadh-quinone oxidoreductase subunit n (52 kDa, Protein from Escherichia coli BL21(DE3))
- 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione (322 Da, Ligand)
- Nadh-quinone oxidoreductase subunit j (17 kDa, Protein from Escherichia coli BL21(DE3))
- Water (18 Da, Ligand)
- Nadh-quinone oxidoreductase subunit h (36 kDa, Protein from Escherichia coli BL21(DE3))
- 1,4-dihydronicotinamide adenine dinucleotide (665 Da, Ligand)
- Nadh-quinone oxidoreductase subunit i (20 kDa, Protein from Escherichia coli BL21(DE3))
- Iron/sulfur cluster (351 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Proton-translocating nadh-quinone oxidoreductase, chain l (66 kDa, Protein from Escherichia coli BL21(DE3))
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Nadh dehydrogenase i subunit e (17 kDa, Protein from Escherichia coli BL21(DE3))
- Flavin mononucleotide (456 Da, Ligand)
- Complex i (Complex from Escherichia coli)
- Nadh-quinone oxidoreductase subunit c/d (68 kDa, Protein from Escherichia coli BL21(DE3))
Complex I from E. coli, DDM/LMNG-purified, Apo, Open state
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 7p7c
Q-score: 0.65
Kravchuk V, Petrova O, Kampjut D, Wojciechowska-Bason A, Breese Z, Sazanov L
Nature (2022) 609 pp. 808-814 [ Pubmed: 36104567 DOI: doi:10.1038/s41586-022-05199-7 ]
- Nadh-quinone oxidoreductase (99 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit k (10 kDa, Protein from Escherichia coli BL21(DE3))
- Eicosane (282 Da, Ligand)
- Nadh-quinone oxidoreductase subunit f (48 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh dehydrogenase i subunit m (56 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit b (25 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit a (16 kDa, Protein from Escherichia coli BL21(DE3))
- Calcium ion (40 Da, Ligand)
- Nadh-quinone oxidoreductase subunit n (52 kDa, Protein from Escherichia coli BL21(DE3))
- Ubiquinone-8 (727 Da, Ligand)
- Nadh-quinone oxidoreductase subunit j (17 kDa, Protein from Escherichia coli BL21(DE3))
- Water (18 Da, Ligand)
- Nadh-quinone oxidoreductase subunit h (36 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit i (20 kDa, Protein from Escherichia coli BL21(DE3))
- Iron/sulfur cluster (351 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Proton-translocating nadh-quinone oxidoreductase, chain l (66 kDa, Protein from Escherichia coli BL21(DE3))
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Nadh dehydrogenase i subunit e (17 kDa, Protein from Escherichia coli BL21(DE3))
- Flavin mononucleotide (456 Da, Ligand)
- Complex i (Complex from Escherichia coli)
- Nadh-quinone oxidoreductase subunit c/d (68 kDa, Protein from Escherichia coli BL21(DE3))
Complex I from E. coli, DDM/LMNG-purified, Apo, Resting state
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7p7e
Q-score: 0.64
Kravchuk V, Petrova O, Kampjut D, Wojciechowska-Bason A, Breese Z, Sazanov L
Nature (2022) 609 pp. 808-814 [ Pubmed: 36104567 DOI: doi:10.1038/s41586-022-05199-7 ]
- Nadh-quinone oxidoreductase (99 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit k (10 kDa, Protein from Escherichia coli BL21(DE3))
- Eicosane (282 Da, Ligand)
- Nadh-quinone oxidoreductase subunit j (19 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh dehydrogenase i subunit m (56 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit b (25 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit a (16 kDa, Protein from Escherichia coli BL21(DE3))
- Calcium ion (40 Da, Ligand)
- Nadh-quinone oxidoreductase subunit n (52 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit c/d (68 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit i (16 kDa, Protein from Escherichia coli BL21(DE3))
- Water (18 Da, Ligand)
- Nadh-quinone oxidoreductase subunit h (36 kDa, Protein from Escherichia coli BL21(DE3))
- Iron/sulfur cluster (351 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Proton-translocating nadh-quinone oxidoreductase, chain l (66 kDa, Protein from Escherichia coli BL21(DE3))
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Nadh dehydrogenase i subunit e (17 kDa, Protein from Escherichia coli BL21(DE3))
- Flavin mononucleotide (456 Da, Ligand)
- Complex i (Complex from Escherichia coli)
- Nadh-quinone oxidoreductase subunit f (48 kDa, Protein from Escherichia coli BL21(DE3))
Complex I from E. coli, DDM/LMNG-purified, with DQ, Open state
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7p7j
Q-score: 0.608
Kravchuk V, Petrova O, Kampjut D, Wojciechowska-Bason A, Breese Z, Sazanov L
Nature (2022) 609 pp. 808-814 [ Pubmed: 36104567 DOI: doi:10.1038/s41586-022-05199-7 ]
- Nadh-quinone oxidoreductase (99 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit k (10 kDa, Protein from Escherichia coli BL21(DE3))
- Eicosane (282 Da, Ligand)
- Nadh-quinone oxidoreductase subunit f (48 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit j (19 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh dehydrogenase i subunit m (56 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit b (25 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit a (16 kDa, Protein from Escherichia coli BL21(DE3))
- Calcium ion (40 Da, Ligand)
- Nadh-quinone oxidoreductase subunit n (52 kDa, Protein from Escherichia coli BL21(DE3))
- 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione (322 Da, Ligand)
- Nadh-quinone oxidoreductase subunit h (36 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit i (20 kDa, Protein from Escherichia coli BL21(DE3))
- Iron/sulfur cluster (351 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Proton-translocating nadh-quinone oxidoreductase, chain l (66 kDa, Protein from Escherichia coli BL21(DE3))
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Nadh dehydrogenase i subunit e (17 kDa, Protein from Escherichia coli BL21(DE3))
- Flavin mononucleotide (456 Da, Ligand)
- Complex i (Complex from Escherichia coli)
- Nadh-quinone oxidoreductase subunit c/d (68 kDa, Protein from Escherichia coli BL21(DE3))
Complex I from E. coli, DDM/LMNG-purified, under Turnover at pH 8, Closed state
Resolution: 2.93 Å
EM Method: Single-particle
Fitted PDBs: 7z80
Q-score: 0.583
Kravchuk V, Petrova O, Kampjut D, Wojciechowska-Bason A, Breese Z, Sazanov L
Nature (2022) 609 pp. 808-814 [ Pubmed: 36104567 DOI: doi:10.1038/s41586-022-05199-7 ]
- Complex i (Complex from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit k (10 kDa, Protein from Escherichia coli BL21(DE3))
- Eicosane (282 Da, Ligand)
- Nadh-quinone oxidoreductase subunit j (19 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh dehydrogenase i subunit m (56 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit b (25 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit a (16 kDa, Protein from Escherichia coli BL21(DE3))
- Calcium ion (40 Da, Ligand)
- Nadh-quinone oxidoreductase subunit n (52 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit f (49 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit cd (68 kDa, Protein from Escherichia coli BL21(DE3))
- 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione (322 Da, Ligand)
- Nadh dehydrogenase i subunit e (18 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit h (36 kDa, Protein from Escherichia coli BL21(DE3))
- 1,4-dihydronicotinamide adenine dinucleotide (665 Da, Ligand)
- Nadh-quinone oxidoreductase subunit i (20 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit l (66 kDa, Protein from Escherichia coli BL21(DE3))
- Iron/sulfur cluster (351 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Nadh-quinone oxidoreductase (100 kDa, Protein from Escherichia coli BL21(DE3))
- Flavin mononucleotide (456 Da, Ligand)
Complex I from E. coli, DDM/LMNG-purified, under Turnover at pH 8, Open state
Resolution: 2.88 Å
EM Method: Single-particle
Fitted PDBs: 7z83
Q-score: 0.577
Kravchuk V, Petrova O, Kampjut D, Wojciechowska-Bason A, Breese Z, Sazanov L
Nature (2022) 609 pp. 808-814 [ DOI: doi:10.1038/s41586-022-05199-7 Pubmed: 36104567 ]
- Flavin mononucleotide (456 Da, Ligand)
- Nadh-quinone oxidoreductase subunit j (19 kDa, Protein from Escherichia coli BL21(DE3))
- Eicosane (282 Da, Ligand)
- Nadh dehydrogenase i subunit m (56 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase (100 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit b (25 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit i (20 kDa, Protein from Escherichia coli BL21(DE3))
- 1,4-dihydronicotinamide adenine dinucleotide (665 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Nadh-quinone oxidoreductase subunit k (10 kDa, Protein from Escherichia coli BL21(DE3))
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Nadh-quinone oxidoreductase subunit f (49 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit n (52 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit h (36 kDa, Protein from Escherichia coli BL21(DE3))
- Iron/sulfur cluster (351 Da, Ligand)
- Nadh-quinone oxidoreductase subunit a (16 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit cd (68 kDa, Protein from Escherichia coli BL21(DE3))
- Complex i (Complex from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit l (66 kDa, Protein from Escherichia coli BL21(DE3))
- Calcium ion (40 Da, Ligand)
- Nadh dehydrogenase i subunit e (18 kDa, Protein from Escherichia coli BL21(DE3))
Complex I from E. coli, DDM/LMNG-purified, under Turnover at pH 8, Open-ready state
Resolution: 2.87 Å
EM Method: Single-particle
Fitted PDBs: 7z84
Q-score: 0.591
Kravchuk V, Petrova O, Kampjut D, Wojciechowska-Bason A, Breese Z, Sazanov L
Nature (2022) 609 pp. 808-814 [ DOI: doi:10.1038/s41586-022-05199-7 Pubmed: 36104567 ]
- Flavin mononucleotide (456 Da, Ligand)
- Nadh-quinone oxidoreductase subunit j (19 kDa, Protein from Escherichia coli BL21(DE3))
- Eicosane (282 Da, Ligand)
- Nadh dehydrogenase i subunit m (56 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase (100 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit b (25 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit i (20 kDa, Protein from Escherichia coli BL21(DE3))
- 1,4-dihydronicotinamide adenine dinucleotide (665 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Nadh-quinone oxidoreductase subunit k (10 kDa, Protein from Escherichia coli BL21(DE3))
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Nadh-quinone oxidoreductase subunit f (49 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit n (52 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit h (36 kDa, Protein from Escherichia coli BL21(DE3))
- Iron/sulfur cluster (351 Da, Ligand)
- Nadh-quinone oxidoreductase subunit a (16 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit cd (68 kDa, Protein from Escherichia coli BL21(DE3))
- Complex i (Complex from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit l (66 kDa, Protein from Escherichia coli BL21(DE3))
- Calcium ion (40 Da, Ligand)
- Nadh dehydrogenase i subunit e (18 kDa, Protein from Escherichia coli BL21(DE3))
Complex I from E. coli, LMNG-purified, under Turnover at pH 6, Resting state
Resolution: 2.69 Å
EM Method: Single-particle
Fitted PDBs: 7zci
Q-score: 0.637
Kravchuk V, Petrova O, Kampjut D, Wojciechowska-Bason A, Breese Z, Sazanov L
Nature (2022) 609 pp. 808-814 [ DOI: doi:10.1038/s41586-022-05199-7 Pubmed: 36104567 ]
- Flavin mononucleotide (456 Da, Ligand)
- Complex i (Complex from Escherichia coli)
- Nadh dehydrogenase subunit l (66 kDa, Protein from Escherichia coli)
- Eicosane (282 Da, Ligand)
- 1,4-dihydronicotinamide adenine dinucleotide (665 Da, Ligand)
- Nadh dehydrogenase i subunit m (56 kDa, Protein from Escherichia coli)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Nadh-quinone oxidoreductase subunit k (10 kDa, Protein from Escherichia coli)
- Nadh-quinone oxidoreductase subunit h (36 kDa, Protein from Escherichia coli)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
- Nadh-quinone oxidoreductase subunit b (25 kDa, Protein from Escherichia coli)
- Nadh-quinone oxidoreductase subunit i (20 kDa, Protein from Escherichia coli)
- Nadh-quinone oxidoreductase subunit f (49 kDa, Protein from Escherichia coli)
- Nadh-quinone oxidoreductase subunit c/d (68 kDa, Protein from Escherichia coli)
- Nadh-quinone oxidoreductase (100 kDa, Protein from Escherichia coli)
- Nadh dehydrogenase i subunit e (18 kDa, Protein from Escherichia coli)
- Nadh-quinone oxidoreductase subunit j (19 kDa, Protein from Escherichia coli)
- Nadh-quinone oxidoreductase subunit n (52 kDa, Protein from Escherichia coli)
- Nadh-quinone oxidoreductase subunit a (16 kDa, Protein from Escherichia coli)
- Calcium ion (40 Da, Ligand)