Electron Microscopy Data Bank

EMBL

was set up in 1974 as Europe’s flagship laboratory for the life sciences – an intergovernmental organisation with more than 80 independent research groups covering the spectrum of molecular biology:

Develop

new instruments and methods

Integrating

life science research in Europe

More about EMBL

Six sites

represent EMBL in Europe.

Barcelona

Tissue biology and disease modelling

Grenoble

Structural biology

Hamburg

Structural biology

Heidelberg

Main laboratory

Hinxton

EMBL-EBI: European Bioinformatics Institute

Rome

Epigenetics and neurobiology

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sample_name:"IRON/SULFUR CLUSTER" AND natural_source_ncbi_code:"469008" AND natural_source_ncbi_code:"511693"

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EMD-14620

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Complex I from E. coli, DDM/LMNG-purified, under Turnover at pH 8, Resting state

Release date: Sept. 21, 2022
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7zc5
Q-score: 0.571

Kravchuk V, Petrova O, Kampjut D, Wojciechowska-Bason A, Breese Z, Sazanov L

Nature (2022) 609 pp. 808-814 [ Pubmed: 36104567 DOI: doi:10.1038/s41586-022-05199-7 ]

Sample:

Nadh dehydrogenase i subunit e (18 kDa, Protein from Escherichia coli BL21)
Nadh-quinone oxidoreductase subunit b (25 kDa, Protein from Escherichia coli BL21)
Eicosane (282 Da, Ligand)
Nadh-quinone oxidoreductase subunit f (49 kDa, Protein from Escherichia coli BL21)
Nadh-quinone oxidoreductase subunit l (66 kDa, Protein from Escherichia coli BL21)
Nadh-quinone oxidoreductase subunit n (52 kDa, Protein from Escherichia coli BL21)
Nadh-quinone oxidoreductase subunit cd (68 kDa, Protein from Escherichia coli BL21)
Calcium ion (40 Da, Ligand)
Nadh-quinone oxidoreductase subunit a (16 kDa, Protein from Escherichia coli BL21)
Nadh-quinone oxidoreductase subunit k (10 kDa, Protein from Escherichia coli BL21)
Nadh-quinone oxidoreductase subunit h (36 kDa, Protein from Escherichia coli BL21)
Nadh dehydrogenase i subunit m (56 kDa, Protein from Escherichia coli BL21)
1,4-dihydronicotinamide adenine dinucleotide (665 Da, Ligand)
Iron/sulfur cluster (351 Da, Ligand)
Nadh-quinone oxidoreductase (100 kDa, Protein from Escherichia coli BL21)
Nadh-quinone oxidoreductase subunit j (19 kDa, Protein from Escherichia coli BL21)
Fe2/s2 (inorganic) cluster (175 Da, Ligand)
Nadh-quinone oxidoreductase subunit i (20 kDa, Protein from Escherichia coli BL21)
1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
Flavin mononucleotide (456 Da, Ligand)
Complex i (Complex from Escherichia coli)

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