Resolution: 2.49 Å
EM Method: Single-particle
Fitted PDBs: 8pk8
Q-score: 0.661
Schulz V, Steinhilper R, Oltmanns J, Freibert SA, Krapoth N, Linne U, Welsch S, Hoock MH, Schunemann V, Murphy BJ, Lill R
Nat Commun (2024) 15 pp. 3269-3269 [ Pubmed: 38627381 DOI: doi:10.1038/s41467-024-47310-8 ]
- Cysteine desulfurase (44 kDa, Protein from Homo sapiens)
- Pyridoxal-5'-phosphate (247 Da, Ligand)
- Iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Iron-bound human nfs1-isd11-acp1-iscu2-fxn (fe-niaux) complex during persulfide transfer (Complex from Homo sapiens)
- Frataxin mature form (14 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
Resolution: 2.58 Å
EM Method: Single-particle
Fitted PDBs: 8pk9
Q-score: 0.661
Schulz V, Steinhilper R, Oltmanns J, Freibert SA, Krapoth N, Linne U, Welsch S, Hoock MH, Schunemann V, Murphy BJ, Lill R
Nat Commun (2024) 15 pp. 3269-3269 [ DOI: doi:10.1038/s41467-024-47310-8 Pubmed: 38627381 ]
- Pyridoxal-5'-phosphate (247 Da, Ligand)
- Iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Frataxin mature form (14 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Water (18 Da, Ligand)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- Cysteine desulfurase (44 kDa, Protein from Homo sapiens)
- Iron-bound human nfs1-isd11-acp1-iscu2-fxn (fe-niaux) complex during persulfide transfer (Complex from Homo sapiens)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
Resolution: 2.75 Å
EM Method: Single-particle
Fitted PDBs: 8pka
Q-score: 0.624
Schulz V, Steinhilper R, Oltmanns J, Freibert SA, Krapoth N, Linne U, Welsch S, Hoock MH, Schunemann V, Murphy BJ, Lill R
Nat Commun (2024) 15 pp. 3269-3269 [ DOI: doi:10.1038/s41467-024-47310-8 Pubmed: 38627381 ]
- Iron-bound human nfs1-isd11-acp1-iscu2-fxn (fe-niaux) complex during persulfide transfer (Complex)
- Cysteine desulfurase (44 kDa, Protein from Homo sapiens)
- Pyridoxal-5'-phosphate (247 Da, Ligand)
- Fe (ii) ion (55 Da, Ligand)
- Water (18 Da, Ligand)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Cysteine desulfurase, lyr motif-containing protein 4 and iron-sulfur cluster assembly enzyme iscu (Complex from Homo sapiens)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- Acyl carrier protein (Complex from Escherichia coli BL21(DE3))
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
Complex I from E. coli, DDM-purified, Apo, Resting state
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7p62
Q-score: 0.496
Kravchuk V, Petrova O, Kampjut D, Wojciechowska-Bason A, Breese Z, Sazanov L
Nature (2022) 609 pp. 808-814 [ Pubmed: 36104567 DOI: doi:10.1038/s41586-022-05199-7 ]
- Nadh-quinone oxidoreductase (99 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit i (16 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit k (10 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh dehydrogenase i subunit m (56 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit b (25 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit a (16 kDa, Protein from Escherichia coli BL21(DE3))
- Calcium ion (40 Da, Ligand)
- Nadh-quinone oxidoreductase subunit n (52 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit j (18 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit c/d (68 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit h (36 kDa, Protein from Escherichia coli BL21(DE3))
- Iron/sulfur cluster (351 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Proton-translocating nadh-quinone oxidoreductase, chain l (66 kDa, Protein from Escherichia coli BL21(DE3))
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Nadh dehydrogenase i subunit e (17 kDa, Protein from Escherichia coli BL21(DE3))
- Flavin mononucleotide (456 Da, Ligand)
- Complex i (Complex from Escherichia coli)
- Nadh-quinone oxidoreductase subunit f (48 kDa, Protein from Escherichia coli BL21(DE3))
Complex I from E. coli, DDM/LMNG-purified, under Turnover at pH 6, Closed state
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7p63
Q-score: 0.523
Kravchuk V, Petrova O, Kampjut D, Wojciechowska-Bason A, Breese Z, Sazanov L
Nature (2022) 609 pp. 808-814 [ Pubmed: 36104567 DOI: doi:10.1038/s41586-022-05199-7 ]
- Nadh-quinone oxidoreductase subunit k (10 kDa, Protein from Escherichia coli BL21(DE3))
- Eicosane (282 Da, Ligand)
- Nadh-quinone oxidoreductase subunit j (19 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh dehydrogenase i subunit m (56 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit b (25 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit a (16 kDa, Protein from Escherichia coli BL21(DE3))
- Calcium ion (40 Da, Ligand)
- Nadh-quinone oxidoreductase subunit n (52 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit f (49 kDa, Protein from Escherichia coli BL21(DE3))
- 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione (322 Da, Ligand)
- Nadh dehydrogenase i subunit e (18 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit h (36 kDa, Protein from Escherichia coli BL21(DE3))
- 1,4-dihydronicotinamide adenine dinucleotide (665 Da, Ligand)
- Nadh-quinone oxidoreductase subunit i (20 kDa, Protein from Escherichia coli BL21(DE3))
- Iron/sulfur cluster (351 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Proton-translocating nadh-quinone oxidoreductase, chain l (66 kDa, Protein from Escherichia coli BL21(DE3))
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Nadh-quinone oxidoreductase (100 kDa, Protein from Escherichia coli BL21(DE3))
- Flavin mononucleotide (456 Da, Ligand)
- Complex i (Complex from Escherichia coli)
- Nadh-quinone oxidoreductase subunit c/d (68 kDa, Protein from Escherichia coli BL21(DE3))
Complex I from E. coli, DDM/LMNG-purified, under Turnover at pH 6, Open state
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 7p64
Q-score: 0.666
Kravchuk V, Petrova O, Kampjut D, Wojciechowska-Bason A, Breese Z, Sazanov L
Nature (2022) 609 pp. 808-814 [ Pubmed: 36104567 DOI: doi:10.1038/s41586-022-05199-7 ]
- Nadh-quinone oxidoreductase (99 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit k (10 kDa, Protein from Escherichia coli BL21(DE3))
- Eicosane (282 Da, Ligand)
- Nadh-quinone oxidoreductase subunit f (48 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh dehydrogenase i subunit m (56 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit b (25 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit a (16 kDa, Protein from Escherichia coli BL21(DE3))
- Calcium ion (40 Da, Ligand)
- Nadh-quinone oxidoreductase subunit n (52 kDa, Protein from Escherichia coli BL21(DE3))
- 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione (322 Da, Ligand)
- Nadh-quinone oxidoreductase subunit j (17 kDa, Protein from Escherichia coli BL21(DE3))
- Water (18 Da, Ligand)
- Nadh-quinone oxidoreductase subunit h (36 kDa, Protein from Escherichia coli BL21(DE3))
- 1,4-dihydronicotinamide adenine dinucleotide (665 Da, Ligand)
- Nadh-quinone oxidoreductase subunit i (20 kDa, Protein from Escherichia coli BL21(DE3))
- Iron/sulfur cluster (351 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Proton-translocating nadh-quinone oxidoreductase, chain l (66 kDa, Protein from Escherichia coli BL21(DE3))
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Nadh dehydrogenase i subunit e (17 kDa, Protein from Escherichia coli BL21(DE3))
- Flavin mononucleotide (456 Da, Ligand)
- Complex i (Complex from Escherichia coli)
- Nadh-quinone oxidoreductase subunit c/d (68 kDa, Protein from Escherichia coli BL21(DE3))
Complex I from E. coli, DDM/LMNG-purified, Apo, Open state
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 7p7c
Q-score: 0.65
Kravchuk V, Petrova O, Kampjut D, Wojciechowska-Bason A, Breese Z, Sazanov L
Nature (2022) 609 pp. 808-814 [ Pubmed: 36104567 DOI: doi:10.1038/s41586-022-05199-7 ]
- Nadh-quinone oxidoreductase (99 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit k (10 kDa, Protein from Escherichia coli BL21(DE3))
- Eicosane (282 Da, Ligand)
- Nadh-quinone oxidoreductase subunit f (48 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh dehydrogenase i subunit m (56 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit b (25 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit a (16 kDa, Protein from Escherichia coli BL21(DE3))
- Calcium ion (40 Da, Ligand)
- Nadh-quinone oxidoreductase subunit n (52 kDa, Protein from Escherichia coli BL21(DE3))
- Ubiquinone-8 (727 Da, Ligand)
- Nadh-quinone oxidoreductase subunit j (17 kDa, Protein from Escherichia coli BL21(DE3))
- Water (18 Da, Ligand)
- Nadh-quinone oxidoreductase subunit h (36 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit i (20 kDa, Protein from Escherichia coli BL21(DE3))
- Iron/sulfur cluster (351 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Proton-translocating nadh-quinone oxidoreductase, chain l (66 kDa, Protein from Escherichia coli BL21(DE3))
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Nadh dehydrogenase i subunit e (17 kDa, Protein from Escherichia coli BL21(DE3))
- Flavin mononucleotide (456 Da, Ligand)
- Complex i (Complex from Escherichia coli)
- Nadh-quinone oxidoreductase subunit c/d (68 kDa, Protein from Escherichia coli BL21(DE3))
Complex I from E. coli, DDM/LMNG-purified, Apo, Resting state
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7p7e
Q-score: 0.64
Kravchuk V, Petrova O, Kampjut D, Wojciechowska-Bason A, Breese Z, Sazanov L
Nature (2022) 609 pp. 808-814 [ Pubmed: 36104567 DOI: doi:10.1038/s41586-022-05199-7 ]
- Nadh-quinone oxidoreductase (99 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit k (10 kDa, Protein from Escherichia coli BL21(DE3))
- Eicosane (282 Da, Ligand)
- Nadh-quinone oxidoreductase subunit j (19 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh dehydrogenase i subunit m (56 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit b (25 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit a (16 kDa, Protein from Escherichia coli BL21(DE3))
- Calcium ion (40 Da, Ligand)
- Nadh-quinone oxidoreductase subunit n (52 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit c/d (68 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit i (16 kDa, Protein from Escherichia coli BL21(DE3))
- Water (18 Da, Ligand)
- Nadh-quinone oxidoreductase subunit h (36 kDa, Protein from Escherichia coli BL21(DE3))
- Iron/sulfur cluster (351 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Proton-translocating nadh-quinone oxidoreductase, chain l (66 kDa, Protein from Escherichia coli BL21(DE3))
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Nadh dehydrogenase i subunit e (17 kDa, Protein from Escherichia coli BL21(DE3))
- Flavin mononucleotide (456 Da, Ligand)
- Complex i (Complex from Escherichia coli)
- Nadh-quinone oxidoreductase subunit f (48 kDa, Protein from Escherichia coli BL21(DE3))
Complex I from E. coli, DDM/LMNG-purified, with DQ, Open state
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7p7j
Q-score: 0.608
Kravchuk V, Petrova O, Kampjut D, Wojciechowska-Bason A, Breese Z, Sazanov L
Nature (2022) 609 pp. 808-814 [ Pubmed: 36104567 DOI: doi:10.1038/s41586-022-05199-7 ]
- Nadh-quinone oxidoreductase (99 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit k (10 kDa, Protein from Escherichia coli BL21(DE3))
- Eicosane (282 Da, Ligand)
- Nadh-quinone oxidoreductase subunit f (48 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit j (19 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh dehydrogenase i subunit m (56 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit b (25 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit a (16 kDa, Protein from Escherichia coli BL21(DE3))
- Calcium ion (40 Da, Ligand)
- Nadh-quinone oxidoreductase subunit n (52 kDa, Protein from Escherichia coli BL21(DE3))
- 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione (322 Da, Ligand)
- Nadh-quinone oxidoreductase subunit h (36 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit i (20 kDa, Protein from Escherichia coli BL21(DE3))
- Iron/sulfur cluster (351 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Proton-translocating nadh-quinone oxidoreductase, chain l (66 kDa, Protein from Escherichia coli BL21(DE3))
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Nadh dehydrogenase i subunit e (17 kDa, Protein from Escherichia coli BL21(DE3))
- Flavin mononucleotide (456 Da, Ligand)
- Complex i (Complex from Escherichia coli)
- Nadh-quinone oxidoreductase subunit c/d (68 kDa, Protein from Escherichia coli BL21(DE3))
Complex I from E. coli, DDM/LMNG-purified, with DQ, Resting state
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7p7k
Q-score: 0.589
Kravchuk V, Petrova O, Kampjut D, Wojciechowska-Bason A, Breese Z, Sazanov L
Nature (2022) 609 pp. 808-814 [ Pubmed: 36104567 DOI: doi:10.1038/s41586-022-05199-7 ]
- Nadh-quinone oxidoreductase subunit i (16 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase (99 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit k (10 kDa, Protein from Escherichia coli BL21(DE3))
- Eicosane (282 Da, Ligand)
- Nadh-quinone oxidoreductase subunit f (48 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh dehydrogenase i subunit m (56 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit b (25 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit a (16 kDa, Protein from Escherichia coli BL21(DE3))
- Calcium ion (40 Da, Ligand)
- Nadh-quinone oxidoreductase subunit n (52 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit j (17 kDa, Protein from Escherichia coli BL21(DE3))
- Nadh-quinone oxidoreductase subunit h (36 kDa, Protein from Escherichia coli BL21(DE3))
- Iron/sulfur cluster (351 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Proton-translocating nadh-quinone oxidoreductase, chain l (66 kDa, Protein from Escherichia coli BL21(DE3))
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Nadh dehydrogenase i subunit e (17 kDa, Protein from Escherichia coli BL21(DE3))
- Flavin mononucleotide (456 Da, Ligand)
- Complex i (Complex from Escherichia coli)
- Nadh-quinone oxidoreductase subunit c/d (68 kDa, Protein from Escherichia coli BL21(DE3))