Electron Microscopy Data Bank

EMBL

was set up in 1974 as Europe’s flagship laboratory for the life sciences – an intergovernmental organisation with more than 80 independent research groups covering the spectrum of molecular biology:

Develop

new instruments and methods

Integrating

life science research in Europe

More about EMBL

Six sites

represent EMBL in Europe.

Barcelona

Tissue biology and disease modelling

Grenoble

Structural biology

Hamburg

Structural biology

Heidelberg

Main laboratory

Hinxton

EMBL-EBI: European Bioinformatics Institute

Rome

Epigenetics and neurobiology

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sample_name:"IRON/SULFUR CLUSTER" AND resolution:[4 TO 5} AND sample_type:"other" AND release_date:[2023-01-01T00:00:00Z TO 2023-12-31T23:59:59Z]

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EMD-29873

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Complete DNA elongation subcomplex of Xenopus laevis DNA polymerase alpha-primase

Release date: April 12, 2023
Resolution: 4.4 Å
EM Method: Single-particle
Fitted PDBs: 8g9o
Q-score: 0.275

Mullins EA, Salay LE, Durie CL, Bradley NP, Jackman JE, Ohi MD, Chazin WJ, Eichman BF

Nat Struct Mol Biol (2024) 31 pp. 777-790 [ DOI: doi:10.1038/s41594-024-01227-4 Pubmed: 38491139 ]

Sample:

Iron/sulfur cluster (351 Da, Ligand)
Primase (Complex from Xenopus laevis)
Dna polymerase alpha catalytic subunit (129 kDa, Protein from Xenopus laevis)
Dna primase large subunit (59 kDa, Protein from Xenopus laevis)
Polymerase alpha (Complex from Xenopus laevis)
Dna template (15 kDa, DNA from synthetic construct)
Rna-dna primer (6 kDa, Macromolecule from synthetic construct)
2'-deoxyguanosine-5'-triphosphate (507 Da, Ligand)
Magnesium ion (24 Da, Ligand)
Dna elongation substrate (20 kDa, Complex from synthetic construct)
Polymerase alpha-primase with a dna elongation substrate (110 kDa, Complex)

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