Human polymerase delta holoenzyme Conformer 1
Resolution: 4.27 Å
EM Method: Single-particle
Fitted PDBs: 6s1m
Q-score: 0.239
Lancey C, Tehseen M, Raducanu VS, Rashid F, Merino N, Ragan TJ, Savva CG, Zaher MS, Shirbini A, Blanco FJ, Hamdan SM, De Biasio A
Nat Commun (2020) 11 pp. 1109-1109 [ Pubmed: 32111820 DOI: doi:10.1038/s41467-020-14898-6 ]
- Proliferating cell nuclear antigen (29 kDa, Protein from Homo sapiens)
- Dna primer (7 kDa, DNA from synthetic construct)
- Pold holoenzyme (Complex)
- Iron/sulfur cluster (351 Da, Ligand)
- Dna polymerase delta subunit 3 (52 kDa, Protein from Homo sapiens)
- Dna polymerase delta subunit 2 (51 kDa, Protein from Homo sapiens)
- Dna (Complex from synthetic construct)
- Dna polymerase delta subunit 4 (16 kDa, Protein from Homo sapiens)
- Thymidine-5'-triphosphate (482 Da, Ligand)
- Human polymerase delta (Complex from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Dna template (11 kDa, DNA from synthetic construct)
- Proliferating cell nuclear antigen (Complex from Homo sapiens)
- Dna polymerase delta catalytic subunit (123 kDa, Protein from Homo sapiens)
Human polymerase delta holoenzyme Conformer 2
Resolution: 4.86 Å
EM Method: Single-particle
Fitted PDBs: 6s1n
Q-score: 0.162
Lancey C, Tehseen M, Raducanu VS, Rashid F, Merino N, Ragan TJ, Savva CG, Zaher MS, Shirbini A, Blanco FJ, Hamdan SM, De Biasio A
Nat Commun (2020) 11 pp. 1109-1109 [ Pubmed: 32111820 DOI: doi:10.1038/s41467-020-14898-6 ]
- Proliferating cell nuclear antigen (29 kDa, Protein from Homo sapiens)
- Dna primer (7 kDa, DNA from synthetic construct)
- Iron/sulfur cluster (351 Da, Ligand)
- Pold holoenzyme (Complex)
- Dna polymerase delta subunit 3 (52 kDa, Protein from Homo sapiens)
- Dna polymerase delta subunit 2 (51 kDa, Protein from Homo sapiens)
- Dna (Complex from synthetic construct)
- Dna polymerase delta subunit 4 (16 kDa, Protein from Homo sapiens)
- Thymidine-5'-triphosphate (482 Da, Ligand)
- Human polymerase delta (Complex from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Dna template (11 kDa, DNA from synthetic construct)
- Proliferating cell nuclear antigen (Complex from Homo sapiens)
- Dna polymerase delta catalytic subunit (123 kDa, Protein from Homo sapiens)
Human polymerase delta holoenzyme Conformer 3
Resolution: 8.1 Å
EM Method: Single-particle
Fitted PDBs: 6s1o
Q-score: 0.094
Lancey C, Tehseen M, Raducanu VS, Rashid F, Merino N, Ragan TJ, Savva CG, Zaher MS, Shirbini A, Blanco FJ, Hamdan SM, De Biasio A
Nat Commun (2020) 11 pp. 1109-1109 [ Pubmed: 32111820 DOI: doi:10.1038/s41467-020-14898-6 ]
- Thymidine-5'-triphosphate (482 Da, Ligand)
- Dna polymerase delta catalytic subunit (123 kDa, Protein from Homo sapiens)
- Dna (Complex from synthetic construct)
- Proliferating cell nuclear antigen (29 kDa, Protein from Homo sapiens)
- Iron/sulfur cluster (351 Da, Ligand)
- Human polymerase delta (Complex from Homo sapiens)
- Dna polymerase delta subunit 2 (51 kDa, Protein from Homo sapiens)
- Dna polymerase delta subunit 4 (16 kDa, Protein from Homo sapiens)
- Pold holoenzyme (Complex)
- Dna primer (7 kDa, DNA from synthetic construct)
- Zinc ion (65 Da, Ligand)
- Dna polymerase delta subunit 3 (52 kDa, Protein from Homo sapiens)
- Proliferating cell nuclear antigen (Complex from Homo sapiens)
- Dna template (11 kDa, DNA from synthetic construct)
Processive human polymerase delta holoenzyme
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 6tny
Q-score: 0.442
Lancey C, Tehseen M, Raducanu VS, Rashid F, Merino N, Ragan TJ, Savva CG, Zaher MS, Shirbini A, Blanco FJ, Hamdan SM, De Biasio A
Nat Commun (2020) 11 pp. 1109-1109 [ Pubmed: 32111820 DOI: doi:10.1038/s41467-020-14898-6 ]
- Proliferating cell nuclear antigen (29 kDa, Protein from Homo sapiens)
- Dna primer (7 kDa, DNA from synthetic construct)
- Iron/sulfur cluster (351 Da, Ligand)
- Dna primer, template (Complex from synthetic construct)
- Dna polymerase delta subunit 3 (52 kDa, Protein from Homo sapiens)
- Dna polymerase delta subunit 2 (51 kDa, Protein from Homo sapiens)
- Dna polymerase delta subunit 4 (16 kDa, Protein from Homo sapiens)
- Toolbelt (Complex)
- Thymidine-5'-triphosphate (482 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Dna template (11 kDa, DNA from synthetic construct)
- Dna polymerase (Complex from Homo sapiens)
- Proliferating cell nuclear antigen (Complex from Homo sapiens)
- Dna polymerase delta catalytic subunit (123 kDa, Protein from Homo sapiens)
Human polymerase delta-FEN1-PCNA toolbelt
Resolution: 4.05 Å
EM Method: Single-particle
Fitted PDBs: 6tnz
Q-score: 0.283
Lancey C, Tehseen M, Raducanu VS, Rashid F, Merino N, Ragan TJ, Savva CG, Zaher MS, Shirbini A, Blanco FJ, Hamdan SM, De Biasio A
Nat Commun (2020) 11 pp. 1109-1109 [ Pubmed: 32111820 DOI: doi:10.1038/s41467-020-14898-6 ]
- Flap endonuclease 1 (42 kDa, Protein from Homo sapiens)
- Thymidine-5'-triphosphate (482 Da, Ligand)
- Dna polymerase delta catalytic subunit (123 kDa, Protein from Homo sapiens)
- Dna polymerase (Complex from Homo sapiens)
- Proliferating cell nuclear antigen, flap endonuclease (Complex from Homo sapiens)
- Proliferating cell nuclear antigen (29 kDa, Protein from Homo sapiens)
- Iron/sulfur cluster (351 Da, Ligand)
- Dna template (11 kDa, DNA from synthetic construct)
- Dna polymerase delta subunit 2 (51 kDa, Protein from Homo sapiens)
- Dna polymerase delta subunit 4 (16 kDa, Protein from Homo sapiens)
- Toolbelt (Complex)
- Dna primer (7 kDa, DNA from synthetic construct)
- Zinc ion (65 Da, Ligand)
- Dna polymerase delta subunit 3 (52 kDa, Protein from Homo sapiens)
- Dna primer, template (Complex from synthetic construct)