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EMBL

was set up in 1974 as Europe’s flagship laboratory for the life sciences – an intergovernmental organisation with more than 80 independent research groups covering the spectrum of molecular biology:

Research:

perform basic research in molecular biology

Services:

offer vital services to scientists in the member states

Training

scientists, students and visitors at all levels

Transfer

and development of technology

Develop

new instruments and methods

Integrating

life science research in Europe

More about EMBL

Six sites

represent EMBL in Europe.

Barcelona

Tissue biology and disease modelling

Grenoble

Structural biology

Hamburg

Structural biology

Heidelberg

Main laboratory

Hinxton

EMBL-EBI: European Bioinformatics Institute

Rome

Epigenetics and neurobiology

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sample_name:"IRON/SULFUR CLUSTER" AND xref_links:"cpx" AND xref_links:"alphafold" AND author_name:"Burnside C" AND author_orcid:"0000-0002-9373-4737"

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EMD-27250

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Yeast mitochondrial small subunit assembly intermediate (State 2)

Release date: Dec. 21, 2022
Resolution: 3.13 Å
EM Method: Single-particle
Fitted PDBs: 8d8k
Q-score: 0.508

Harper NJ, Burnside C, Klinge S

Nature (2023) 614 pp. 175-181 [ DOI: doi:10.1038/s41586-022-05621-0 Pubmed: 36482135 ]

Sample:

37s ribosomal protein s7, mitochondrial (27 kDa, Protein from baker's yeast)
37s ribosomal protein s9, mitochondrial (31 kDa, Protein from baker's yeast)
37s ribosomal protein sws2, mitochondrial (16 kDa, Protein from baker's yeast)
37s ribosomal protein mrp13, mitochondrial (39 kDa, Protein from baker's yeast)
37s ribosomal protein rsm18, mitochondrial (15 kDa, Protein from baker's yeast)
37s ribosomal protein mrp10, mitochondrial (10 kDa, Protein from baker's yeast)
37s ribosomal protein s16, mitochondrial (13 kDa, Protein from baker's yeast)
37s ribosomal protein mrp4, mitochondrial (44 kDa, Protein from baker's yeast)
37s ribosomal protein mrp17, mitochondrial (15 kDa, Protein from baker's yeast)
37s ribosomal protein s26, mitochondrial (30 kDa, Protein from baker's yeast)
37s ribosomal protein mrp1, mitochondrial (36 kDa, Protein from baker's yeast)
37s ribosomal protein s19, mitochondrial (10 kDa, Protein from baker's yeast)
Mrps12 isoform 1 (16 kDa, Protein from baker's yeast)
37s ribosomal protein mrp2, mitochondrial (13 kDa, Protein from baker's yeast)
15s ribosomal rna (549 kDa, RNA from baker's yeast)
Mitochondrial 37s ribosomal protein s27 (12 kDa, Protein from baker's yeast)
37s ribosomal protein mrp51, mitochondrial (39 kDa, Protein from baker's yeast)
37s ribosomal protein s23, mitochondrial (50 kDa, Protein from baker's yeast)
Yeast mitochondrial small subunit assembly intermediate (state 2) (1 MDa, Complex from Saccharomyces cerevisiae)
37s ribosomal protein s28, mitochondrial (33 kDa, Protein from baker's yeast)
37s ribosomal protein s10, mitochondrial (23 kDa, Protein from baker's yeast)
37s ribosomal protein pet123, mitochondrial (36 kDa, Protein from baker's yeast)
Adenosine-5'-triphosphate (507 Da, Ligand)
Protein fyv4, mitochondrial (15 kDa, Protein from baker's yeast)
37s ribosomal protein s25, mitochondrial (30 kDa, Protein from baker's yeast)
Water (18 Da, Ligand)
37s ribosomal protein mrp21, mitochondrial (20 kDa, Protein from baker's yeast)
37s ribosomal protein s17, mitochondrial (27 kDa, Protein from baker's yeast)
Probable s-adenosyl-l-methionine-dependent rna methyltransferase rsm22, mitochondrial (72 kDa, Protein from baker's yeast)
Magnesium ion (24 Da, Ligand)
Iron/sulfur cluster (351 Da, Ligand)
37s ribosomal protein s18, mitochondrial (24 kDa, Protein from baker's yeast)
37s ribosomal protein s24, mitochondrial (37 kDa, Protein from baker's yeast)
37s ribosomal protein nam9, mitochondrial (56 kDa, Protein from baker's yeast)
37s ribosomal protein s5, mitochondrial (34 kDa, Protein from baker's yeast)
37s ribosomal protein s8, mitochondrial (17 kDa, Protein from baker's yeast)
Ribosomal protein var1, mitochondrial (47 kDa, Protein from baker's yeast)
Unknown protein sequence (8 kDa, Protein from baker's yeast)
37s ribosomal protein s35, mitochondrial (39 kDa, Protein from baker's yeast)
Mitochondrial group i intron splicing factor ccm1 (101 kDa, Protein from baker's yeast)

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EMD-26966

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Human mitochondrial small subunit assembly intermediate (State A)

Release date: Dec. 14, 2022
Resolution: 2.66 Å
EM Method: Single-particle
Fitted PDBs: 8csp
Q-score: 0.548

Harper NJ, Burnside C, Klinge S

Nature (2023) 614 pp. 175-181 [ DOI: doi:10.1038/s41586-022-05621-0 Pubmed: 36482135 ]

Sample:

28s ribosomal protein s34, mitochondrial (25 kDa, Protein from Homo sapiens)
28s ribosomal protein s27, mitochondrial (47 kDa, Protein from Homo sapiens)
Nitric oxide-associated protein 1 (78 kDa, Protein from Homo sapiens)
12s mitochondrial rrna (306 kDa, RNA from Homo sapiens)
28s ribosomal protein s9, mitochondrial (45 kDa, Protein from Homo sapiens)
28s ribosomal protein s31, mitochondrial (45 kDa, Protein from Homo sapiens)
Malonyl-coa-acyl carrier protein transacylase, mitochondrial (43 kDa, Protein from Homo sapiens)
Human mitochondrial small subunit assembly intermediate, state a (1 MDa, Complex from Homo sapiens)
Gtpase era, mitochondrial (48 kDa, Protein from Homo sapiens)
28s ribosomal protein s12, mitochondrial (15 kDa, Protein from Homo sapiens)
28s ribosomal protein s6, mitochondrial (14 kDa, Protein from Homo sapiens)
28s ribosomal protein s26, mitochondrial (24 kDa, Protein from Homo sapiens)
Pentatricopeptide repeat domain-containing protein 3, mitochondrial (78 kDa, Protein from Homo sapiens)
28s ribosomal protein s29, mitochondrial (45 kDa, Protein from Homo sapiens)
S-adenosylmethionine (398 Da, Ligand)
28s ribosomal protein s23, mitochondrial (21 kDa, Protein from Homo sapiens)
Guanosine-5'-diphosphate (443 Da, Ligand)
Dimethyladenosine transferase 1, mitochondrial (39 kDa, Protein from Homo sapiens)
Potassium ion (39 Da, Ligand)
28s ribosomal protein s10, mitochondrial (23 kDa, Protein from Homo sapiens)
28s ribosomal protein s17, mitochondrial (14 kDa, Protein from Homo sapiens)
Zinc ion (65 Da, Ligand)
Methyltransferase-like protein 17, mitochondrial (50 kDa, Protein from Homo sapiens)
28s ribosomal protein s33, mitochondrial (12 kDa, Protein from Homo sapiens)
28s ribosomal protein s25, mitochondrial (20 kDa, Protein from Homo sapiens)
Adenosine-5'-triphosphate (507 Da, Ligand)
28s ribosomal protein s5, mitochondrial (48 kDa, Protein from Homo sapiens)
28s ribosomal protein s2, mitochondrial (33 kDa, Protein from Homo sapiens)
28s ribosomal protein s24, mitochondrial (19 kDa, Protein from Homo sapiens)
28s ribosomal protein s35, mitochondrial (36 kDa, Protein from Homo sapiens)
Magnesium ion (24 Da, Ligand)
28s ribosomal protein s28, mitochondrial (20 kDa, Protein from Homo sapiens)
28s ribosomal protein s7, mitochondrial (28 kDa, Protein from Homo sapiens)
28s ribosomal protein s16, mitochondrial (15 kDa, Protein from Homo sapiens)
Iron/sulfur cluster (351 Da, Ligand)
Fe2/s2 (inorganic) cluster (175 Da, Ligand)
28s ribosomal protein s15, mitochondrial (29 kDa, Protein from Homo sapiens)
28s ribosomal protein s22, mitochondrial (41 kDa, Protein from Homo sapiens)
28s ribosomal protein s14, mitochondrial (15 kDa, Protein from Homo sapiens)
28s ribosomal protein s18b, mitochondrial (29 kDa, Protein from Homo sapiens)
28s ribosomal protein s18c, mitochondrial (15 kDa, Protein from Homo sapiens)
28s ribosomal protein s21, mitochondrial (10 kDa, Protein from Homo sapiens)

[Show more]

EMD-26967

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Human mitochondrial small subunit assembly intermediate (State B)

Release date: Dec. 14, 2022
Resolution: 2.54 Å
EM Method: Single-particle
Fitted PDBs: 8csq
Q-score: 0.566

Harper NJ, Burnside C, Klinge S

Nature (2023) 614 pp. 175-181 [ DOI: doi:10.1038/s41586-022-05621-0 Pubmed: 36482135 ]

Sample:

28s ribosomal protein s34, mitochondrial (25 kDa, Protein from Homo sapiens)
28s ribosomal protein s27, mitochondrial (47 kDa, Protein from Homo sapiens)
12s mitochondrial rrna (306 kDa, RNA from Homo sapiens)
28s ribosomal protein s9, mitochondrial (45 kDa, Protein from Homo sapiens)
28s ribosomal protein s31, mitochondrial (45 kDa, Protein from Homo sapiens)
Malonyl-coa-acyl carrier protein transacylase, mitochondrial (43 kDa, Protein from Homo sapiens)
Gtpase era, mitochondrial (48 kDa, Protein from Homo sapiens)
28s ribosomal protein s21, mitochondrial (10 kDa, Protein from Homo sapiens)
28s ribosomal protein s12, mitochondrial (15 kDa, Protein from Homo sapiens)
28s ribosomal protein s6, mitochondrial (14 kDa, Protein from Homo sapiens)
28s ribosomal protein s26, mitochondrial (24 kDa, Protein from Homo sapiens)
Pentatricopeptide repeat domain-containing protein 3, mitochondrial (78 kDa, Protein from Homo sapiens)
28s ribosomal protein s29, mitochondrial (45 kDa, Protein from Homo sapiens)
S-adenosylmethionine (398 Da, Ligand)
28s ribosomal protein s23, mitochondrial (21 kDa, Protein from Homo sapiens)
Guanosine-5'-diphosphate (443 Da, Ligand)
Dimethyladenosine transferase 1, mitochondrial (39 kDa, Protein from Homo sapiens)
Potassium ion (39 Da, Ligand)
28s ribosomal protein s10, mitochondrial (23 kDa, Protein from Homo sapiens)
28s ribosomal protein s17, mitochondrial (14 kDa, Protein from Homo sapiens)
Zinc ion (65 Da, Ligand)
Methyltransferase-like protein 17, mitochondrial (50 kDa, Protein from Homo sapiens)
Aurora kinase a-interacting protein (22 kDa, Protein from Homo sapiens)
28s ribosomal protein s33, mitochondrial (12 kDa, Protein from Homo sapiens)
28s ribosomal protein s25, mitochondrial (20 kDa, Protein from Homo sapiens)
Adenosine-5'-triphosphate (507 Da, Ligand)
28s ribosomal protein s5, mitochondrial (48 kDa, Protein from Homo sapiens)
28s ribosomal protein s2, mitochondrial (33 kDa, Protein from Homo sapiens)
28s ribosomal protein s24, mitochondrial (19 kDa, Protein from Homo sapiens)
28s ribosomal protein s35, mitochondrial (36 kDa, Protein from Homo sapiens)
Magnesium ion (24 Da, Ligand)
28s ribosomal protein s28, mitochondrial (20 kDa, Protein from Homo sapiens)
28s ribosomal protein s7, mitochondrial (28 kDa, Protein from Homo sapiens)
28s ribosomal protein s16, mitochondrial (15 kDa, Protein from Homo sapiens)
Iron/sulfur cluster (351 Da, Ligand)
Fe2/s2 (inorganic) cluster (175 Da, Ligand)
28s ribosomal protein s15, mitochondrial (29 kDa, Protein from Homo sapiens)
28s ribosomal protein s22, mitochondrial (41 kDa, Protein from Homo sapiens)
28s ribosomal protein s14, mitochondrial (15 kDa, Protein from Homo sapiens)
28s ribosomal protein s18b, mitochondrial (29 kDa, Protein from Homo sapiens)
28s ribosomal protein s18c, mitochondrial (15 kDa, Protein from Homo sapiens)
Human mitochondrial small subunit assembly intermediate, state b (1 MDa, Complex from Homo sapiens)

[Show more]

EMD-26968

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Human mitochondrial small subunit assembly intermediate (State C)

Release date: Dec. 14, 2022
Resolution: 2.54 Å
EM Method: Single-particle
Fitted PDBs: 8csr
Q-score: 0.563

Harper NJ, Burnside C, Klinge S

Nature (2023) 614 pp. 175-181 [ DOI: doi:10.1038/s41586-022-05621-0 Pubmed: 36482135 ]

Sample:

28s ribosomal protein s24, mitochondrial (19 kDa, Protein from human)
Putative ribosome-binding factor a, mitochondrial (38 kDa, Protein from human)
Aurora kinase a-interacting protein (22 kDa, Protein from human)
28s ribosomal protein s2, mitochondrial (33 kDa, Protein from human)
28s ribosomal protein s7, mitochondrial (28 kDa, Protein from human)
Malonyl-coa-acyl carrier protein transacylase, mitochondrial (43 kDa, Protein from human)
28s ribosomal protein s25, mitochondrial (20 kDa, Protein from human)
28s ribosomal protein s33, mitochondrial (12 kDa, Protein from human)
28s ribosomal protein s18b, mitochondrial (29 kDa, Protein from human)
28s ribosomal protein s12, mitochondrial (15 kDa, Protein from human)
28s ribosomal protein s10, mitochondrial (23 kDa, Protein from human)
Nicotinamide-adenine-dinucleotide (663 Da, Ligand)
Methyltransferase-like protein 17, mitochondrial (50 kDa, Protein from human)
28s ribosomal protein s11, mitochondrial (20 kDa, Protein from human)
28s ribosomal protein s15, mitochondrial (29 kDa, Protein from human)
S-adenosylmethionine (398 Da, Ligand)
Pentatricopeptide repeat domain-containing protein 3, mitochondrial (78 kDa, Protein from human)
Guanosine-5'-diphosphate (443 Da, Ligand)
Potassium ion (39 Da, Ligand)
28s ribosomal protein s22, mitochondrial (41 kDa, Protein from human)
28s ribosomal protein s21, mitochondrial (10 kDa, Protein from human)
Zinc ion (65 Da, Ligand)
28s ribosomal protein s34, mitochondrial (25 kDa, Protein from human)
28s ribosomal protein s9, mitochondrial (45 kDa, Protein from human)
12s mitochondrial rrna (306 kDa, RNA from human)
28s ribosomal protein s29, mitochondrial (45 kDa, Protein from human)
Adenosine-5'-triphosphate (507 Da, Ligand)
28s ribosomal protein s17, mitochondrial (14 kDa, Protein from human)
28s ribosomal protein s26, mitochondrial (24 kDa, Protein from human)
28s ribosomal protein s18c, mitochondrial (15 kDa, Protein from human)
Water (18 Da, Ligand)
28s ribosomal protein s35, mitochondrial (36 kDa, Protein from human)
28s ribosomal protein s16, mitochondrial (15 kDa, Protein from human)
28s ribosomal protein s27, mitochondrial (47 kDa, Protein from human)
Magnesium ion (24 Da, Ligand)
28s ribosomal protein s14, mitochondrial (15 kDa, Protein from human)
Iron/sulfur cluster (351 Da, Ligand)
Fe2/s2 (inorganic) cluster (175 Da, Ligand)
Human mitochondrial small subunit assembly intermediate, state c (1 MDa, Complex from Homo sapiens)
28s ribosomal protein s23, mitochondrial (21 kDa, Protein from human)
28s ribosomal protein s31, mitochondrial (45 kDa, Protein from human)
28s ribosomal protein s6, mitochondrial (14 kDa, Protein from human)
28s ribosomal protein s28, mitochondrial (20 kDa, Protein from human)
Dimethyladenosine transferase 1, mitochondrial (39 kDa, Protein from human)
28s ribosomal protein s5, mitochondrial (48 kDa, Protein from human)

[Show more]

EMD-26969

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Human mitochondrial small subunit assembly intermediate (State D)

Release date: Dec. 14, 2022
Resolution: 2.36 Å
EM Method: Single-particle
Fitted PDBs: 8css
Q-score: 0.576

Harper NJ, Burnside C, Klinge S

Nature (2023) 614 pp. 175-181 [ Pubmed: 36482135 DOI: doi:10.1038/s41586-022-05621-0 ]

Sample:

Potassium ion (39 Da, Ligand)
S-adenosylmethionine (398 Da, Ligand)
28s ribosomal protein s27, mitochondrial (47 kDa, Protein from human)
Malonyl-coa-acyl carrier protein transacylase, mitochondrial (43 kDa, Protein from human)
28s ribosomal protein s31, mitochondrial (45 kDa, Protein from human)
Adenosine-5'-triphosphate (507 Da, Ligand)
28s ribosomal protein s26, mitochondrial (24 kDa, Protein from human)
28s ribosomal protein s35, mitochondrial (36 kDa, Protein from human)
Putative ribosome-binding factor a, mitochondrial (38 kDa, Protein from human)
Magnesium ion (24 Da, Ligand)
Pentatricopeptide repeat domain-containing protein 3, mitochondrial (78 kDa, Protein from human)
28s ribosomal protein s21, mitochondrial (10 kDa, Protein from human)
28s ribosomal protein s6, mitochondrial (14 kDa, Protein from human)
28s ribosomal protein s18c, mitochondrial (15 kDa, Protein from human)
Fe2/s2 (inorganic) cluster (175 Da, Ligand)
28s ribosomal protein s33, mitochondrial (12 kDa, Protein from human)
28s ribosomal protein s12, mitochondrial (15 kDa, Protein from human)
Methyltransferase-like protein 17, mitochondrial (50 kDa, Protein from human)
28s ribosomal protein s18b, mitochondrial (29 kDa, Protein from human)
Nicotinamide-adenine-dinucleotide (663 Da, Ligand)
28s ribosomal protein s29, mitochondrial (45 kDa, Protein from human)
28s ribosomal protein s9, mitochondrial (45 kDa, Protein from human)
Iron/sulfur cluster (351 Da, Ligand)
Guanosine-5'-diphosphate (443 Da, Ligand)
28s ribosomal protein s17, mitochondrial (14 kDa, Protein from human)
28s ribosomal protein s5, mitochondrial (48 kDa, Protein from human)
28s ribosomal protein s7, mitochondrial (28 kDa, Protein from human)
28s ribosomal protein s2, mitochondrial (33 kDa, Protein from human)
28s ribosomal protein s10, mitochondrial (23 kDa, Protein from human)
28s ribosomal protein s22, mitochondrial (41 kDa, Protein from human)
28s ribosomal protein s28, mitochondrial (20 kDa, Protein from human)
12s mitochondrial rrna (306 kDa, RNA from human)
28s ribosomal protein s14, mitochondrial (15 kDa, Protein from human)
Zinc ion (65 Da, Ligand)
28s ribosomal protein s11, mitochondrial (20 kDa, Protein from human)
28s ribosomal protein s34, mitochondrial (25 kDa, Protein from human)
28s ribosomal protein s16, mitochondrial (15 kDa, Protein from human)
28s ribosomal protein s25, mitochondrial (20 kDa, Protein from human)
Water (18 Da, Ligand)
Aurora kinase a-interacting protein (22 kDa, Protein from human)
28s ribosomal protein s24, mitochondrial (19 kDa, Protein from human)
28s ribosomal protein s15, mitochondrial (29 kDa, Protein from human)
28s ribosomal protein s23, mitochondrial (21 kDa, Protein from human)
Human mitochondrial small subunit assembly intermediate, state d (1 MDa, Complex from Homo sapiens)

[Show more]

EMD-26970

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Human mitochondrial small subunit assembly intermediate (State E)

Release date: Dec. 14, 2022
Resolution: 2.85 Å
EM Method: Single-particle
Fitted PDBs: 8cst
Q-score: 0.555

Harper NJ, Burnside C, Klinge S

Nature (2023) 614 pp. 175-181 [ DOI: doi:10.1038/s41586-022-05621-0 Pubmed: 36482135 ]

Sample:

28s ribosomal protein s24, mitochondrial (19 kDa, Protein from human)
Putative ribosome-binding factor a, mitochondrial (38 kDa, Protein from human)
Aurora kinase a-interacting protein (22 kDa, Protein from human)
28s ribosomal protein s2, mitochondrial (33 kDa, Protein from human)
28s ribosomal protein s7, mitochondrial (28 kDa, Protein from human)
28s ribosomal protein s25, mitochondrial (20 kDa, Protein from human)
28s ribosomal protein s33, mitochondrial (12 kDa, Protein from human)
28s ribosomal protein s18b, mitochondrial (29 kDa, Protein from human)
28s ribosomal protein s12, mitochondrial (15 kDa, Protein from human)
28s ribosomal protein s10, mitochondrial (23 kDa, Protein from human)
Nicotinamide-adenine-dinucleotide (663 Da, Ligand)
Methyltransferase-like protein 17, mitochondrial (50 kDa, Protein from human)
28s ribosomal protein s11, mitochondrial (20 kDa, Protein from human)
28s ribosomal protein s15, mitochondrial (29 kDa, Protein from human)
S-adenosylmethionine (398 Da, Ligand)
Pentatricopeptide repeat domain-containing protein 3, mitochondrial (78 kDa, Protein from human)
Guanosine-5'-diphosphate (443 Da, Ligand)
Potassium ion (39 Da, Ligand)
28s ribosomal protein s22, mitochondrial (41 kDa, Protein from human)
Human mitochondrial small subunit assembly intermediate, state e (1 MDa, Complex from Homo sapiens)
28s ribosomal protein s21, mitochondrial (10 kDa, Protein from human)
Zinc ion (65 Da, Ligand)
28s ribosomal protein s34, mitochondrial (25 kDa, Protein from human)
12s mitochondrial rrna (306 kDa, RNA from human)
28s ribosomal protein s9, mitochondrial (45 kDa, Protein from human)
28s ribosomal protein s29, mitochondrial (45 kDa, Protein from human)
Adenosine-5'-triphosphate (507 Da, Ligand)
28s ribosomal protein s17, mitochondrial (14 kDa, Protein from human)
28s ribosomal protein s26, mitochondrial (24 kDa, Protein from human)
28s ribosomal protein s18c, mitochondrial (15 kDa, Protein from human)
28s ribosomal protein s16, mitochondrial (15 kDa, Protein from human)
28s ribosomal protein s27, mitochondrial (47 kDa, Protein from human)
Magnesium ion (24 Da, Ligand)
28s ribosomal protein s14, mitochondrial (15 kDa, Protein from human)
Iron/sulfur cluster (351 Da, Ligand)
Fe2/s2 (inorganic) cluster (175 Da, Ligand)
28s ribosomal protein s23, mitochondrial (21 kDa, Protein from human)
28s ribosomal protein s31, mitochondrial (45 kDa, Protein from human)
28s ribosomal protein s6, mitochondrial (14 kDa, Protein from human)
28s ribosomal protein s28, mitochondrial (20 kDa, Protein from human)
28s ribosomal protein s35, mitochondrial (36 kDa, Protein from human)
28s ribosomal protein s5, mitochondrial (48 kDa, Protein from human)

[Show more]

EMD-26971

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Human mitochondrial small subunit assembly intermediate (State C*)

Release date: Dec. 14, 2022
Resolution: 3.03 Å
EM Method: Single-particle
Fitted PDBs: 8csu
Q-score: 0.519

Harper NJ, Burnside C, Klinge S

Nature (2023) 614 pp. 175-181 [ DOI: doi:10.1038/s41586-022-05621-0 Pubmed: 36482135 ]

Sample:

28s ribosomal protein s24, mitochondrial (19 kDa, Protein from human)
Putative ribosome-binding factor a, mitochondrial (38 kDa, Protein from human)
Aurora kinase a-interacting protein (22 kDa, Protein from human)
28s ribosomal protein s2, mitochondrial (33 kDa, Protein from human)
28s ribosomal protein s7, mitochondrial (28 kDa, Protein from human)
28s ribosomal protein s25, mitochondrial (20 kDa, Protein from human)
28s ribosomal protein s33, mitochondrial (12 kDa, Protein from human)
28s ribosomal protein s18b, mitochondrial (29 kDa, Protein from human)
28s ribosomal protein s12, mitochondrial (15 kDa, Protein from human)
28s ribosomal protein s10, mitochondrial (23 kDa, Protein from human)
Nicotinamide-adenine-dinucleotide (663 Da, Ligand)
Methyltransferase-like protein 17, mitochondrial (50 kDa, Protein from human)
28s ribosomal protein s11, mitochondrial (20 kDa, Protein from human)
28s ribosomal protein s15, mitochondrial (29 kDa, Protein from human)
S-adenosylmethionine (398 Da, Ligand)
Pentatricopeptide repeat domain-containing protein 3, mitochondrial (78 kDa, Protein from human)
Guanosine-5'-diphosphate (443 Da, Ligand)
Potassium ion (39 Da, Ligand)
28s ribosomal protein s22, mitochondrial (41 kDa, Protein from human)
28s ribosomal protein s21, mitochondrial (10 kDa, Protein from human)
Zinc ion (65 Da, Ligand)
Human mitochondrial small subunit assembly intermediate, state c* (1 MDa, Complex from Homo sapiens)
28s ribosomal protein s34, mitochondrial (25 kDa, Protein from human)
28s ribosomal protein s9, mitochondrial (45 kDa, Protein from human)
12s mitochondrial rrna (306 kDa, RNA from human)
28s ribosomal protein s29, mitochondrial (45 kDa, Protein from human)
Adenosine-5'-triphosphate (507 Da, Ligand)
28s ribosomal protein s17, mitochondrial (14 kDa, Protein from human)
28s ribosomal protein s26, mitochondrial (24 kDa, Protein from human)
28s ribosomal protein s18c, mitochondrial (15 kDa, Protein from human)
28s ribosomal protein s35, mitochondrial (36 kDa, Protein from human)
28s ribosomal protein s16, mitochondrial (15 kDa, Protein from human)
28s ribosomal protein s27, mitochondrial (47 kDa, Protein from human)
Magnesium ion (24 Da, Ligand)
28s ribosomal protein s14, mitochondrial (15 kDa, Protein from human)
Iron/sulfur cluster (351 Da, Ligand)
Fe2/s2 (inorganic) cluster (175 Da, Ligand)
28s ribosomal protein s23, mitochondrial (21 kDa, Protein from human)
28s ribosomal protein s31, mitochondrial (45 kDa, Protein from human)
28s ribosomal protein s6, mitochondrial (14 kDa, Protein from human)
28s ribosomal protein s28, mitochondrial (20 kDa, Protein from human)
Dimethyladenosine transferase 1, mitochondrial (39 kDa, Protein from human)
28s ribosomal protein s5, mitochondrial (48 kDa, Protein from human)

[Show more]

EMD-27251

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Yeast mitochondrial small subunit assembly intermediate (State 3)

Release date: Dec. 14, 2022
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 8d8l
Q-score: 0.614

Harper NJ, Burnside C, Klinge S

Nature (2023) 614 pp. 175-181 [ DOI: doi:10.1038/s41586-022-05621-0 Pubmed: 36482135 ]

Sample:

37s ribosomal protein s7, mitochondrial (27 kDa, Protein from baker's yeast)
37s ribosomal protein s9, mitochondrial (31 kDa, Protein from baker's yeast)
3-hydroxyisobutyryl-coa hydrolase, mitochondrial (56 kDa, Protein from baker's yeast)
37s ribosomal protein sws2, mitochondrial (16 kDa, Protein from baker's yeast)
37s ribosomal protein mrp13, mitochondrial (39 kDa, Protein from baker's yeast)
37s ribosomal protein rsm18, mitochondrial (15 kDa, Protein from baker's yeast)
37s ribosomal protein mrp10, mitochondrial (10 kDa, Protein from baker's yeast)
37s ribosomal protein s16, mitochondrial (13 kDa, Protein from baker's yeast)
37s ribosomal protein mrp4, mitochondrial (44 kDa, Protein from baker's yeast)
37s ribosomal protein mrp17, mitochondrial (15 kDa, Protein from baker's yeast)
37s ribosomal protein s26, mitochondrial (30 kDa, Protein from baker's yeast)
37s ribosomal protein mrp1, mitochondrial (36 kDa, Protein from baker's yeast)
37s ribosomal protein s19, mitochondrial (10 kDa, Protein from baker's yeast)
37s ribosomal protein mrp2, mitochondrial (13 kDa, Protein from baker's yeast)
15s ribosomal rna (549 kDa, RNA from baker's yeast)
37s ribosomal protein mrp51, mitochondrial (39 kDa, Protein from baker's yeast)
Mitochondrial 37s ribosomal protein s27 (12 kDa, Protein from baker's yeast)
37s ribosomal protein s23, mitochondrial (50 kDa, Protein from baker's yeast)
37s ribosomal protein s28, mitochondrial (33 kDa, Protein from baker's yeast)
37s ribosomal protein s10, mitochondrial (23 kDa, Protein from baker's yeast)
37s ribosomal protein pet123, mitochondrial (36 kDa, Protein from baker's yeast)
Adenosine-5'-triphosphate (507 Da, Ligand)
Protein fyv4, mitochondrial (15 kDa, Protein from baker's yeast)
37s ribosomal protein s25, mitochondrial (30 kDa, Protein from baker's yeast)
Water (18 Da, Ligand)
37s ribosomal protein mrp21, mitochondrial (20 kDa, Protein from baker's yeast)
37s ribosomal protein s17, mitochondrial (27 kDa, Protein from baker's yeast)
Probable s-adenosyl-l-methionine-dependent rna methyltransferase rsm22, mitochondrial (72 kDa, Protein from baker's yeast)
Magnesium ion (24 Da, Ligand)
Iron/sulfur cluster (351 Da, Ligand)
37s ribosomal protein s18, mitochondrial (24 kDa, Protein from baker's yeast)
37s ribosomal protein s24, mitochondrial (37 kDa, Protein from baker's yeast)
37s ribosomal protein nam9, mitochondrial (56 kDa, Protein from baker's yeast)
37s ribosomal protein s12, mitochondrial (16 kDa, Protein from baker's yeast)
37s ribosomal protein s5, mitochondrial (34 kDa, Protein from baker's yeast)
37s ribosomal protein s8, mitochondrial (17 kDa, Protein from baker's yeast)
Ribosomal protein var1, mitochondrial (47 kDa, Protein from baker's yeast)
Yeast mitochondrial small subunit assembly intermediate (state 3) (1 MDa, Complex from Saccharomyces cerevisiae)
Unknown protein sequence (8 kDa, Protein from baker's yeast)
37s ribosomal protein s35, mitochondrial (39 kDa, Protein from baker's yeast)

[Show more]

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