Cryo-EM structure of a Pyrococcus abyssi 30S bound to Met-initiator tRNA, mRNA, aIF1A and aIF5B
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7zah
Q-score: 0.591
Kazan R, Bourgeois G, Lazennec-Schurdevin C, Larquet E, Mechulam Y, Coureux PD, Schmitt E
Nucleic Acids Res (2022) 50 pp. 6532-6548 [ DOI: doi:10.1093/nar/gkac490 Pubmed: 35694843 ]
- Zn-ribbon rna-binding protein involved in translation (7 kDa, Protein from Pyrococcus abyssi GE5)
- Methionine (149 Da, Ligand)
- Mrna (7 kDa, RNA from Pyrococcus abyssi GE5)
- Translation initiation factor 5b (69 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s8e (14 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s19 (15 kDa, Protein from Pyrococcus abyssi GE5)
- Translation initiation factor 1a (15 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s27e (7 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s14 type z (6 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s28e (7 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s10 (11 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s7 (24 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s24e (11 kDa, Protein from Pyrococcus abyssi GE5)
- Initiation complex with 30s ribosomal subunit,trna, mrna and initiation factors 1a and 5b (Complex)
- 30s ribosomal protein s5 (26 kDa, Protein from Pyrococcus abyssi GE5)
- Trna-met, translation initiation factor 1a and translation initiation factor 5b (Complex from Pyrococcus abyssi GE5)
- 30s and 50s ribosomal proteins (Complex from Pyrococcus abyssi GE5)
- 30s ribosomal protein s27ae (5 kDa, Protein from Pyrococcus abyssi GE5)
- Trna-met (24 kDa, RNA from Pyrococcus abyssi GE5)
- 30s ribosomal protein s17 (13 kDa, Protein from Pyrococcus abyssi GE5)
- Zinc ion (65 Da, Ligand)
- Mrna (Complex from Pyrococcus abyssi GE5)
- 30s ribosomal protein s2 (23 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s6e (14 kDa, Protein from Pyrococcus abyssi GE5)
- 50s ribosomal protein l7ae (13 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s12 (16 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s8 (14 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s3ae (22 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s17e (8 kDa, Protein from Pyrococcus abyssi GE5)
- Water (18 Da, Ligand)
- 30s ribosomal protein s3 (23 kDa, Protein from Pyrococcus abyssi GE5)
- Phosphoaminophosphonic acid-guanylate ester (522 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s9 (15 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s4 (21 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s11 (14 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s13 (16 kDa, Protein from Pyrococcus abyssi GE5)
- 50s ribosomal protein l41e (4 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s19e (17 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s4e (28 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s15 (18 kDa, Protein from Pyrococcus abyssi GE5)
- 16s rrna (487 kDa, RNA from Pyrococcus abyssi GE5)
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7zki
Q-score: 0.418
Kazan R, Bourgeois G, Lazennec-Schurdevin C, Larquet E, Mechulam Y, Coureux PD, Schmitt E
Nucleic Acids Res (2022) 50 pp. 6532-6548 [ DOI: doi:10.1093/nar/gkac490 Pubmed: 35694843 ]
- Translation initiation factor 1a and probable translation initiation factor if-2 (Complex from Pyrococcus abyssi GE5)
- Trna-met (Complex from Escherichia coli)
- Methionine (149 Da, Ligand)
- Translation initiation factor 5b (69 kDa, Protein from Pyrococcus abyssi GE5)
- Met-trnaimet (24 kDa, RNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Phosphoaminophosphonic acid-guanylate ester (522 Da, Ligand)
- Translation initiation factor 1a (15 kDa, Protein from Pyrococcus abyssi GE5)
- Trna-aif5b-aif1a moiety of a pyrococcus abyssi translation initiation complex with 30s ribosomal subunit,trna, mrna and initiation factors 1a and 5b. (Complex)