Electron Microscopy Data Bank

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sample_name:"NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1" AND release_date:[2022-01-01T00:00:00Z TO 2022-12-31T23:59:59Z] AND author_orcid:"0000-0002-2902-4677"

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EMD-14132

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Bovine complex I in lipid nanodisc, Active-Q10

Release date: May 25, 2022
Resolution: 2.84 Å
EM Method: Single-particle
Fitted PDBs: 7qsk
Q-score: 0.598

Chung I, Wright JJ, Bridges HR, Ivanov BS, Biner O, Pereira CS, Arantes GM, Hirst J

Nat Commun (2022) 13 pp. 2758-2758 [ DOI: doi:10.1038/s41467-022-30506-1 Pubmed: 35589726 ]

Sample:

Flavin mononucleotide (456 Da, Ligand)
Potassium ion (39 Da, Ligand)
Cardiolipin (1 kDa, Ligand)
Nadh-ubiquinone oxidoreductase chain 4l (10 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 (21 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial (13 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 subunit c2 (14 kDa, Protein from cattle)
Nadh-ubiquinone oxidoreductase chain 6 (19 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (21 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial (42 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 (11 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 (8 kDa, Protein from cattle)
~{s}-[2-[3-[[(2~{r})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{s})-3-oxidanyltetradecanethioate (584 Da, Ligand)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 (16 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 (17 kDa, Protein from cattle)
Nadh-ubiquinone oxidoreductase chain 5 (68 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (23 kDa, Protein from cattle)
1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
Magnesium ion (24 Da, Ligand)
Guanosine-5'-triphosphate (523 Da, Ligand)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 (13 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 (20 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial (11 kDa, Protein from cattle)
Myristic acid (228 Da, Ligand)
Fe2/s2 (inorganic) cluster (175 Da, Ligand)
Nadh-ubiquinone oxidoreductase chain 1 (35 kDa, Protein from cattle)
Acyl carrier protein, mitochondrial (17 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 (15 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 (11 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (19 kDa, Protein from cattle)
1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
Nadh-ubiquinone oxidoreductase chain 4 (52 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (27 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 (14 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial (52 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 (15 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 5 (12 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 (6 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 (15 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 (21 kDa, Protein from cattle)
Nadh-ubiquinone oxidoreductase chain 3 (13 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 (12 kDa, Protein from cattle)
Iron/sulfur cluster (351 Da, Ligand)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 (16 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial (17 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (50 kDa, Protein from cattle)
Mitochondrial complex i in the active state with a ubiquinone-10 bound (1 MDa, Complex from Bos taurus)
Nadh-ubiquinone oxidoreductase 75 kda subunit, mitochondrial (79 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial (12 kDa, Protein from cattle)
Cholic acid (408 Da, Ligand)
Zinc ion (65 Da, Ligand)
Nadh dehydrogenase [ubiquinone] 1 subunit c1, mitochondrial (8 kDa, Protein from cattle)
Ubiquinone-10 (863 Da, Ligand)
Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
Nadh-ubiquinone oxidoreductase chain 2 (39 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial (21 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial (39 kDa, Protein from cattle)
Water (18 Da, Ligand)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial (30 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial (23 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 (9 kDa, Protein from cattle)

[Show more]

EMD-14133

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Bovine complex I in lipid nanodisc, Active-apo

Release date: May 25, 2022
Resolution: 2.76 Å
EM Method: Single-particle
Fitted PDBs: 7qsl
Q-score: 0.625

Chung I, Wright JJ, Bridges HR, Ivanov BS, Biner O, Pereira CS, Arantes GM, Hirst J

Nat Commun (2022) 13 pp. 2758-2758 [ DOI: doi:10.1038/s41467-022-30506-1 Pubmed: 35589726 ]

Sample:

Flavin mononucleotide (456 Da, Ligand)
Potassium ion (39 Da, Ligand)
Cardiolipin (1 kDa, Ligand)
Nadh-ubiquinone oxidoreductase chain 4l (10 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 (21 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial (13 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 subunit c2 (14 kDa, Protein from cattle)
Nadh-ubiquinone oxidoreductase chain 6 (19 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (21 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial (42 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 (11 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 (8 kDa, Protein from cattle)
~{s}-[2-[3-[[(2~{r})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{s})-3-oxidanyltetradecanethioate (584 Da, Ligand)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 (16 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 (17 kDa, Protein from cattle)
Nadh-ubiquinone oxidoreductase chain 5 (68 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (23 kDa, Protein from cattle)
1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
Magnesium ion (24 Da, Ligand)
Guanosine-5'-triphosphate (523 Da, Ligand)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 (13 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 (20 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial (11 kDa, Protein from cattle)
Myristic acid (228 Da, Ligand)
Fe2/s2 (inorganic) cluster (175 Da, Ligand)
Nadh-ubiquinone oxidoreductase chain 1 (35 kDa, Protein from cattle)
Acyl carrier protein, mitochondrial (17 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 (15 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 (11 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (19 kDa, Protein from cattle)
1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
Nadh-ubiquinone oxidoreductase chain 4 (52 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (27 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 (14 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial (52 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 (15 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 5 (12 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 (6 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 (15 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 (21 kDa, Protein from cattle)
Nadh-ubiquinone oxidoreductase chain 3 (13 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 (12 kDa, Protein from cattle)
Iron/sulfur cluster (351 Da, Ligand)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 (16 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial (17 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (50 kDa, Protein from cattle)
Nadh-ubiquinone oxidoreductase 75 kda subunit, mitochondrial (79 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial (12 kDa, Protein from cattle)
Cholic acid (408 Da, Ligand)
Zinc ion (65 Da, Ligand)
Nadh dehydrogenase [ubiquinone] 1 subunit c1, mitochondrial (8 kDa, Protein from cattle)
Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
Mitochondrial complex i in the active state (1 MDa, Complex from Bos taurus)
Nadh-ubiquinone oxidoreductase chain 2 (39 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial (21 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial (39 kDa, Protein from cattle)
Water (18 Da, Ligand)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial (30 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial (23 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 (9 kDa, Protein from cattle)

[Show more]

EMD-14134

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Bovine complex I in lipid nanodisc, Deactive-ligand (composite)

Release date: May 25, 2022
Resolution: 2.3 Å
EM Method: Single-particle
Fitted PDBs: 7qsm
Q-score: 0.709

Chung I, Wright JJ, Bridges HR, Ivanov BS, Biner O, Pereira CS, Arantes GM, Hirst J

Nat Commun (2022) 13 pp. 2758-2758 [ Pubmed: 35589726 DOI: doi:10.1038/s41467-022-30506-1 ]

Sample:

Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial (21 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 (11 kDa, Protein from cattle)
Nadh-ubiquinone oxidoreductase chain 4 (52 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 (16 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 (20 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial (30 kDa, Protein from cattle)
Cardiolipin (1 kDa, Ligand)
Guanosine-5'-triphosphate (523 Da, Ligand)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (23 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 subunit c1, mitochondrial (8 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (19 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial (23 kDa, Protein from cattle)
Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
Cholic acid (408 Da, Ligand)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial (12 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 subunit c2 (14 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (50 kDa, Protein from cattle)
Nadh-ubiquinone oxidoreductase chain 6 (19 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial (13 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (21 kDa, Protein from cattle)
Nadh-ubiquinone oxidoreductase chain 4l (10 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 (16 kDa, Protein from cattle)
Nadh-ubiquinone oxidoreductase chain 2 (39 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 (8 kDa, Protein from cattle)
Potassium ion (39 Da, Ligand)
Zinc ion (65 Da, Ligand)
Myristic acid (228 Da, Ligand)
Nadh-ubiquinone oxidoreductase chain 1 (35 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 (13 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial (11 kDa, Protein from cattle)
Nadh-ubiquinone oxidoreductase 75 kda subunit, mitochondrial (79 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 (15 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 (17 kDa, Protein from cattle)
~{s}-[2-[3-[[(2~{r})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{s})-3-oxidanyltetradecanethioate (584 Da, Ligand)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 (21 kDa, Protein from cattle)
Water (18 Da, Ligand)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial (42 kDa, Protein from cattle)
Acyl carrier protein, mitochondrial (17 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 (11 kDa, Protein from cattle)
Magnesium ion (24 Da, Ligand)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 (15 kDa, Protein from cattle)
Nadh-ubiquinone oxidoreductase chain 5 (68 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 (14 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 (15 kDa, Protein from cattle)
Mitochondrial complex i in the deactive state with a ligand bound (1 MDa, Complex from Bos taurus)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 (21 kDa, Protein from cattle)
Iron/sulfur cluster (351 Da, Ligand)
Fe2/s2 (inorganic) cluster (175 Da, Ligand)
1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
Nadh-ubiquinone oxidoreductase chain 3 (13 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (27 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 (9 kDa, Protein from cattle)
1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 (6 kDa, Protein from cattle)
Flavin mononucleotide (456 Da, Ligand)
Glycerol (92 Da, Ligand)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 (12 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial (39 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 5 (12 kDa, Protein from cattle)
Dodecyl-beta-d-maltoside (510 Da, Ligand)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial (17 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial (52 kDa, Protein from cattle)

[Show more]

EMD-14139

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Bovine complex I in lipid nanodisc, Deactive-apo

Release date: May 25, 2022
Resolution: 2.81 Å
EM Method: Single-particle
Fitted PDBs: 7qsn
Q-score: 0.611

Chung I, Wright JJ, Bridges HR, Ivanov BS, Biner O, Pereira CS, Arantes GM, Hirst J

Nat Commun (2022) 13 pp. 2758-2758 [ Pubmed: 35589726 DOI: doi:10.1038/s41467-022-30506-1 ]

Sample:

Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial (21 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 (11 kDa, Protein from cattle)
Nadh-ubiquinone oxidoreductase chain 4 (52 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 (16 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 (20 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial (30 kDa, Protein from cattle)
Cardiolipin (1 kDa, Ligand)
Guanosine-5'-triphosphate (523 Da, Ligand)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (23 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 subunit c1, mitochondrial (8 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (19 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial (23 kDa, Protein from cattle)
Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
Cholic acid (408 Da, Ligand)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial (12 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 subunit c2 (14 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (50 kDa, Protein from cattle)
Nadh-ubiquinone oxidoreductase chain 6 (19 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial (13 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (21 kDa, Protein from cattle)
Nadh-ubiquinone oxidoreductase chain 4l (10 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 (16 kDa, Protein from cattle)
Nadh-ubiquinone oxidoreductase chain 2 (39 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 (8 kDa, Protein from cattle)
Potassium ion (39 Da, Ligand)
Zinc ion (65 Da, Ligand)
Myristic acid (228 Da, Ligand)
Nadh-ubiquinone oxidoreductase chain 1 (35 kDa, Protein from cattle)
Mitochondrial complex i in the deactive state (1 MDa, Complex from Bos taurus)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 (13 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial (11 kDa, Protein from cattle)
Nadh-ubiquinone oxidoreductase 75 kda subunit, mitochondrial (79 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 (15 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 (17 kDa, Protein from cattle)
~{s}-[2-[3-[[(2~{r})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{s})-3-oxidanyltetradecanethioate (584 Da, Ligand)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 (21 kDa, Protein from cattle)
Water (18 Da, Ligand)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial (42 kDa, Protein from cattle)
Acyl carrier protein, mitochondrial (17 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 (11 kDa, Protein from cattle)
Magnesium ion (24 Da, Ligand)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 (15 kDa, Protein from cattle)
Nadh-ubiquinone oxidoreductase chain 5 (68 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 (14 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 (15 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 (21 kDa, Protein from cattle)
Iron/sulfur cluster (351 Da, Ligand)
Fe2/s2 (inorganic) cluster (175 Da, Ligand)
1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
Nadh-ubiquinone oxidoreductase chain 3 (13 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (27 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 (9 kDa, Protein from cattle)
1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 (6 kDa, Protein from cattle)
Flavin mononucleotide (456 Da, Ligand)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 (12 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial (39 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 5 (12 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial (17 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial (52 kDa, Protein from cattle)

[Show more]

EMD-14140

Download Map 3D View

Bovine complex I in lipid nanodisc, State 3 (Slack)

Release date: May 25, 2022
Resolution: 3.02 Å
EM Method: Single-particle
Fitted PDBs: 7qso
Q-score: 0.567

Chung I, Wright JJ, Bridges HR, Ivanov BS, Biner O, Pereira CS, Arantes GM, Hirst J

Nat Commun (2022) 13 pp. 2758-2758 [ DOI: doi:10.1038/s41467-022-30506-1 Pubmed: 35589726 ]

Sample:

Flavin mononucleotide (456 Da, Ligand)
Potassium ion (39 Da, Ligand)
Cardiolipin (1 kDa, Ligand)
Nadh-ubiquinone oxidoreductase chain 4l (10 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 (21 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial (13 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 subunit c2 (14 kDa, Protein from cattle)
Nadh-ubiquinone oxidoreductase chain 6 (19 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (21 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial (42 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 (11 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 (8 kDa, Protein from cattle)
~{s}-[2-[3-[[(2~{r})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{s})-3-oxidanyltetradecanethioate (584 Da, Ligand)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 (16 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 (17 kDa, Protein from cattle)
Nadh-ubiquinone oxidoreductase chain 5 (68 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (23 kDa, Protein from cattle)
1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
Magnesium ion (24 Da, Ligand)
Guanosine-5'-triphosphate (523 Da, Ligand)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 (13 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 (20 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial (11 kDa, Protein from cattle)
Myristic acid (228 Da, Ligand)
Fe2/s2 (inorganic) cluster (175 Da, Ligand)
Nadh-ubiquinone oxidoreductase chain 1 (35 kDa, Protein from cattle)
Acyl carrier protein, mitochondrial (17 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 (15 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 (11 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (19 kDa, Protein from cattle)
1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
Nadh-ubiquinone oxidoreductase chain 4 (52 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (27 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 (14 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial (52 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 (15 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 5 (12 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 (6 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 (15 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 (21 kDa, Protein from cattle)
Nadh-ubiquinone oxidoreductase chain 3 (13 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 (12 kDa, Protein from cattle)
Iron/sulfur cluster (351 Da, Ligand)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 (16 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial (17 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (50 kDa, Protein from cattle)
Nadh-ubiquinone oxidoreductase 75 kda subunit, mitochondrial (79 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial (12 kDa, Protein from cattle)
Cholic acid (408 Da, Ligand)
Zinc ion (65 Da, Ligand)
Nadh dehydrogenase [ubiquinone] 1 subunit c1, mitochondrial (8 kDa, Protein from cattle)
Ubiquinone-10 (863 Da, Ligand)
Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
Nadh-ubiquinone oxidoreductase chain 2 (39 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial (21 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial (39 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial (30 kDa, Protein from cattle)
Mitochondrial complex i in state 3 with a cholate bound (1 MDa, Complex from Bos taurus)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial (23 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 (9 kDa, Protein from cattle)

[Show more]

EMD-14127

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Bovine complex I in the active state at 3.1 A

Release date: March 2, 2022
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7qsd
Q-score: 0.585

Bridges HR, Blaza JN, Yin Z, Chung I, Pollak MN, Hirst J

Science (2023) 379 pp. 351-357 [ DOI: doi:10.1126/science.ade3332 Pubmed: 36701435 ]

Sample:

Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial (21 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 (11 kDa, Protein from cattle)
Nadh-ubiquinone oxidoreductase chain 4 (52 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 (16 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 (20 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial (30 kDa, Protein from cattle)
Cardiolipin (1 kDa, Ligand)
Guanosine-5'-triphosphate (523 Da, Ligand)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (23 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 subunit c1, mitochondrial (8 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (19 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial (23 kDa, Protein from cattle)
Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial (12 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 subunit c2 (14 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (50 kDa, Protein from cattle)
Nadh-ubiquinone oxidoreductase chain 6 (19 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial (13 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (21 kDa, Protein from cattle)
Nadh-ubiquinone oxidoreductase chain 4l (10 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 (16 kDa, Protein from cattle)
Nadh-ubiquinone oxidoreductase chain 2 (39 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 (8 kDa, Protein from cattle)
Zinc ion (65 Da, Ligand)
Nadh-ubiquinone oxidoreductase chain 1 (35 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 (13 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial (11 kDa, Protein from cattle)
Nadh-ubiquinone oxidoreductase 75 kda subunit, mitochondrial (79 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 (15 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 (17 kDa, Protein from cattle)
~{s}-[2-[3-[[(2~{r})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{s})-3-oxidanyltetradecanethioate (584 Da, Ligand)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 (21 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial (42 kDa, Protein from cattle)
Acyl carrier protein, mitochondrial (17 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 (11 kDa, Protein from cattle)
Magnesium ion (24 Da, Ligand)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 (15 kDa, Protein from cattle)
Nadh-ubiquinone oxidoreductase chain 5 (68 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 (14 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 (15 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 (21 kDa, Protein from cattle)
Iron/sulfur cluster (351 Da, Ligand)
Fe2/s2 (inorganic) cluster (175 Da, Ligand)
1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
Nadh-ubiquinone oxidoreductase chain 3 (13 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (27 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 (9 kDa, Protein from cattle)
1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 (6 kDa, Protein from cattle)
Flavin mononucleotide (456 Da, Ligand)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 (12 kDa, Protein from cattle)
Nadh ubiquinone oxidoreductase (complex i) (1 MDa, Complex from Bos taurus)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial (39 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 5 (12 kDa, Protein from cattle)
Dodecyl-beta-d-maltoside (510 Da, Ligand)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial (17 kDa, Protein from cattle)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial (52 kDa, Protein from cattle)

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