Cryo-EM structure of the Arabidopsis thaliana I+III2 supercomplex (Complete composition)
Resolution: 2.09 Å
EM Method: Single-particle
Fitted PDBs: 8bpx
Q-score: 0.707
Klusch N, Dreimann M, Senkler J, Rugen N, Kuhlbrandt W, Braun HP
Nat Plants (2023) 9 pp. 142-156 [ Pubmed: 36585502 DOI: doi:10.1038/s41477-022-01308-6 ]
- Probable mitochondrial-processing peptidase subunit alpha-1, mitochondrial (54 kDa, Protein from thale cress)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Cytochrome b-c1 complex subunit 10, mitochondrial (5 kDa, Protein from thale cress)
- Uncharacterized protein at2g27730, mitochondrial (11 kDa, Protein from thale cress)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 8-a, mitochondrial (25 kDa, Protein from thale cress)
- Nadh-ubiquinone oxidoreductase chain 1 (36 kDa, Protein from thale cress)
- Ubiquinone-7 (658 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 (15 kDa, Protein from thale cress)
- Ubiquinone-9 (795 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3-a (8 kDa, Protein from thale cress)
- Gamma carbonic anhydrase 2, mitochondrial (30 kDa, Protein from thale cress)
- Water (18 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8-b (11 kDa, Protein from thale cress)
- Fe (iii) ion (55 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
- (1s)-2-{[{[(2r)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl stearate (751 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Gamma carbonic anhydrase-like 2, mitochondrial (27 kDa, Protein from thale cress)
- Esss subunit of nadh:ubiquinone oxidoreductase (complex i) protein (12 kDa, Protein from thale cress)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 5-b (9 kDa, Protein from thale cress)
- Transmembrane protein (9 kDa, Protein from thale cress)
- Outer envelope pore protein 16-3, chloroplastic/mitochondrial (17 kDa, Protein from thale cress)
- At4g16450 (11 kDa, Protein from thale cress)
- Cytochrome b-c1 complex subunit rieske-1, mitochondrial (29 kDa, Protein from thale cress)
- Mitochondrial arabidopsis thaliana i+iii2 supercomplex (complete composition) (Complex from Arabidopsis thaliana)
- Cytochrome c1 2, heme protein, mitochondrial (33 kDa, Protein from thale cress)
- Cardiolipin (1 kDa, Ligand)
- Probable nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial (19 kDa, Protein from thale cress)
- Crotonyl coenzyme a (835 Da, Ligand)
- Cytochrome b-c1 complex subunit 8-1, mitochondrial (8 kDa, Protein from thale cress)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10-b (12 kDa, Protein from thale cress)
- Acyl carrier protein 2, mitochondrial (14 kDa, Protein from thale cress)
- Excitatory amino acid transporter (9 kDa, Protein from thale cress)
- Acyl carrier protein 1, mitochondrial (13 kDa, Protein from thale cress)
- Nadh-ubiquinone oxidoreductase chain 2 (55 kDa, Protein from thale cress)
- At2g31490 protein (8 kDa, Protein from thale cress)
- 2-{[(4-o-alpha-d-glucopyranosyl-alpha-d-glucopyranosyl)oxy]methyl}-4-{[(3beta,9beta,14beta,17beta,25r)-spirost-5-en-3-yl]oxy}butyl 4-o-alpha-d-glucopyranosyl-alpha-d-glucopyranoside (1 kDa, Ligand)
- Nadh-ubiquinone oxidoreductase chain 3 (13 kDa, Protein from thale cress)
- Nadh dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (28 kDa, Protein from thale cress)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 (13 kDa, Protein from thale cress)
- Phosphatidylethanolamine (734 Da, Ligand)
- Flavin mononucleotide (456 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial (43 kDa, Protein from thale cress)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 (10 kDa, Protein from thale cress)
- Uncharacterized protein at1g67785 (7 kDa, Protein from thale cress)
- At1g67350 (11 kDa, Protein from thale cress)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- 2,3-dimethoxy-5-methyl-6-(3,11,15,19-tetramethyl-eicosa-2,6,10,14,18-pentaenyl)-[1,4]benzoquinone (522 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (53 kDa, Protein from thale cress)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 (7 kDa, Protein from thale cress)
- 2fe-2s ferredoxin-like superfamily protein (17 kDa, Protein from thale cress)
- Nadh-ubiquinone oxidoreductase chain 4 (55 kDa, Protein from thale cress)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrial (81 kDa, Protein from thale cress)
- Nadh-ubiquinone oxidoreductase chain 4l (11 kDa, Protein from thale cress)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial (12 kDa, Protein from thale cress)
- Cytochrome b (44 kDa, Protein from thale cress)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 3 (22 kDa, Protein from thale cress)
- Gamma carbonic anhydrase 1, mitochondrial (30 kDa, Protein from thale cress)
- Cytochrome b-c1 complex subunit 7-2, mitochondrial (14 kDa, Protein from thale cress)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Cytochrome b-c1 complex subunit 9, mitochondrial (8 kDa, Protein from thale cress)
- Probable mitochondrial-processing peptidase subunit beta, mitochondrial (59 kDa, Protein from thale cress)
- At2g46540/f11c10.23 (6 kDa, Protein from thale cress)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (13 kDa, Protein from thale cress)
- (7s)-4-hydroxy-n,n,n-trimethyl-9-oxo-7-[(palmitoyloxy)methyl]-3,5,8-trioxa-4-phosphahexacosan-1-aminium 4-oxide (763 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 5 (74 kDa, Protein from thale cress)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-a (16 kDa, Protein from thale cress)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2 (7 kDa, Protein from thale cress)
- 1,2-diacyl-glycerol-3-sn-phosphate (704 Da, Ligand)
- Probable nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 (18 kDa, Protein from thale cress)
- Zinc ion (65 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (17 kDa, Protein from thale cress)
- Cytochrome b-c1 complex subunit 6-1, mitochondrial (7 kDa, Protein from thale cress)
- Nadh-ubiquinone oxidoreductase chain 6 (23 kDa, Protein from thale cress)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial (24 kDa, Protein from thale cress)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 2 (45 kDa, Protein from thale cress)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 (11 kDa, Protein from thale cress)
Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A
Resolution: 3.82 Å
EM Method: Single-particle
Fitted PDBs: 7ak6
Q-score: 0.447
Yin Z, Burger N, Kula-Alwar D, Aksentijevic D, Bridges HR, Prag HA, Grba DN, Viscomi C, James AM, Mottahedin A, Krieg T, Murphy MP, Hirst J
Nat Commun (2021) 12 pp. 707-707 [ DOI: doi:10.1038/s41467-021-20942-w Pubmed: 33514727 ]
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 (13 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 (9 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial (21 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial (42 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] 1 subunit c1, mitochondrial (8 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (24 kDa, Protein from Mouse)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Mouse mitochondrial complex i with nd6-p25l mutation (1 MDa, Complex from Mus musculus)
- 1,2-diacyl-sn-glycero-3-phosphoethanolamine (748 Da, Ligand)
- ~{s}-[2-[3-[[(2~{r})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{s})-3-oxidanyltetradecanethioate (584 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 (16 kDa, Protein from Mouse)
- Acyl carrier protein, mitochondrial (17 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 (7 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] 1 subunit c2 (14 kDa, Protein from Mouse)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (21 kDa, Protein from Mouse)
- Flavin mononucleotide (456 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (50 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 (17 kDa, Protein from Mouse)
- Nadh-ubiquinone oxidoreductase chain 5 (68 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial (30 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 (15 kDa, Protein from Mouse)
- Nadh-ubiquinone oxidoreductase chain 3 (13 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial (40 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial (52 kDa, Protein from Mouse)
- Nadh-ubiquinone oxidoreductase chain 4 (51 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 (20 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 (15 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial (17 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (27 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 (15 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 (12 kDa, Protein from Mouse)
- Mcg5603 (15 kDa, Protein from Mouse)
- Zinc ion (65 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial (11 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 (10 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 (11 kDa, Protein from Mouse)
- Nadh-ubiquinone oxidoreductase chain 2 (38 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 (6 kDa, Protein from Mouse)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Nadh-ubiquinone oxidoreductase 75 kda subunit, mitochondrial (79 kDa, Protein from Mouse)
- Cardiolipin (1 kDa, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 (16 kDa, Protein from Mouse)
- Iron/sulfur cluster (351 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (19 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial (13 kDa, Protein from Mouse)
- Nadh-ubiquinone oxidoreductase chain 6 (18 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 (21 kDa, Protein from Mouse)
- Nadh-ubiquinone oxidoreductase chain 4l (10 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial (11 kDa, Protein from Mouse)
- Nadh-ubiquinone oxidoreductase chain 1 (36 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 5 (12 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 (22 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial (24 kDa, Protein from Mouse)