Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 8q7h
Q-score: 0.39
Horn-Ghetko D, Hopf LVM, Tripathi-Giesgen I, Du J, Kostrhon S, Vu DT, Beier V, Steigenberger B, Prabu JR, Stier L, Bruss EM, Mann M, Xiong Y, Schulman BA
Nat Struct Mol Biol (2024) [ DOI: doi:10.1038/s41594-024-01257-y Pubmed: 38605244 ]
- E3 ubiquitin-protein ligase rbx1 (12 kDa, Protein from Homo sapiens)
- Structure of cul9-rbx1 ubiquitin e3 ligase complex in unneddylated and neddylated conformation - focused cullin dimer (Complex from Homo sapiens)
- Unknown (12 kDa, Protein from Homo sapiens)
- Cullin-9 (281 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Nedd8 (9 kDa, Protein from Homo sapiens)
Structure of Neddylated CUL5 C-terminal region-RBX2-ARIH2*
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7oni
Q-score: 0.464
Kostrhon S, Prabu JR, Baek K, Horn-Ghetko D, von Gronau S, Klugel M, Basquin J, Alpi AF, Schulman BA
Nat Chem Biol (2021) 17 pp. 1075-1083 [ Pubmed: 34518685 DOI: doi:10.1038/s41589-021-00858-8 ]
- Nedd8 (9 kDa, Protein from Homo sapiens)
- Ring-box protein 2 (12 kDa, Protein from Homo sapiens)
- Neddylated cul5 c-terminal region-rbx2-arih2 (Complex from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Cullin-5 (91 kDa, Protein from Homo sapiens)
- E3 ubiquitin-protein ligase arih2 (57 kDa, Protein from Homo sapiens)
Structure of Neddylated CRL5Vif-CBFbeta-ARIH2*-APOBEC3C (A3C fullcomplex consensus )
Kostrhon S, Prabu JR, Baek K, Horn-Ghetko D, von Gronau S, Klugel M, Basquin J, Alpi AF, Schulman BA
Nat Chem Biol (2021) 17 pp. 1075-1083 [ Pubmed: 34518685 DOI: doi:10.1038/s41589-021-00858-8 ]
- Rbx2 (Complex from Homo sapiens)
- Nedd8 (Complex from Homo sapiens)
- Cullin-5 (Complex from Homo sapiens)
- Neddylated cul5 c-terminal region-rbx2-arih2 (Complex)
- Arih2 (Complex from Homo sapiens)
Structure of Neddylated CUL5 C-terminal region-RBX2-ARIH2* ( A3G E3-E3 catalytic focused )
Kostrhon S, Prabu JR, Baek K, Horn-Ghetko D, von Gronau S, Klugel M, Basquin J, Alpi AF, Schulman BA
Nat Chem Biol (2021) 17 pp. 1075-1083 [ Pubmed: 34518685 DOI: doi:10.1038/s41589-021-00858-8 ]
- Arih2 (Complex from Homo sapiens)
- Rbx2 (Complex from Homo sapiens)
- Nedd8 (Complex from Homo sapiens)
- Cullin-5 (Complex from Homo sapiens)
- Neddylated cul5 c-terminal region-rbx2-arih2 (Complex)
Structure of Neddylated CRL5Vif-CBFbeta-ARIH2*-APOBEC3C (A3C consensus )
Kostrhon S, Prabu JR, Baek K, Horn-Ghetko D, von Gronau S, Klugel M, Basquin J, Alpi AF, Schulman BA
Nat Chem Biol (2021) 17 pp. 1075-1083 [ Pubmed: 34518685 DOI: doi:10.1038/s41589-021-00858-8 ]
- Rbx2 (Complex from Homo sapiens)
- Nedd8 (Complex from Homo sapiens)
- Cullin-5 (Complex from Homo sapiens)
- Neddylated cul5 c-terminal region-rbx2-arih2 (Complex)
- Arih2 (Complex from Homo sapiens)
Structure of Neddylated CRL5Vif-CBFbeta-ARIH2*-APOBEC3G ( A3G consensus )
Kostrhon S, Prabu JR, Baek K, Horn-Ghetko D, von Gronau S, Klugel M, Basquin J, Alpi AF, Schulman BA
Nat Chem Biol (2021) 17 pp. 1075-1083 [ Pubmed: 34518685 DOI: doi:10.1038/s41589-021-00858-8 ]
- Arih2 (Complex from Homo sapiens)
- Rbx2 (Complex from Homo sapiens)
- Nedd8 (Complex from Homo sapiens)
- Cullin-5 (Complex from Homo sapiens)
- Neddylated cul5 c-terminal region-rbx2-arih2 (Complex)
Horn-Ghetko D, Krist DT, Prabu JR, Baek K, Mulder MPC, Klugel M, Scott DC, Ovaa H, Kleiger G, Schulman BA
Nature (2021) 590 pp. 671-676 [ Pubmed: 33536622 DOI: doi:10.1038/s41586-021-03197-9 ]
Horn-Ghetko D, Krist DT, Prabu JR, Baek K, Mulder MPC, Klugel M, Scott DC, Ovaa H, Kleiger G, Schulman BA
Nature (2021) 590 pp. 671-676 [ DOI: doi:10.1038/s41586-021-03197-9 Pubmed: 33536622 ]
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7b5l
Q-score: 0.427
Horn-Ghetko D, Krist DT, Prabu JR, Baek K, Mulder MPC, Klugel M, Scott DC, Ovaa H, Kleiger G, Schulman BA
Nature (2021) 590 pp. 671-676 [ Pubmed: 33536622 DOI: doi:10.1038/s41586-021-03197-9 ]
- Nedd8-skp1-skp2-ckshs1-cyclin a-cdk2-p27-ube2l3~ub~arih1. transition state 1 composite map. (Complex from Homo sapiens)
- Cullin-1 (89 kDa, Protein from Homo sapiens)
- S-phase kinase-associated protein 2 (47 kDa, Protein from Homo sapiens)
- Nedd8 (8 kDa, Protein from Homo sapiens)
- Cyclin-a2 (48 kDa, Protein from Homo sapiens)
- Cyclin-dependent kinases regulatory subunit 1 (8 kDa, Protein from Homo sapiens)
- E3 ubiquitin-protein ligase arih1 (64 kDa, Protein from Homo sapiens)
- E3 ubiquitin-protein ligase rbx1 (12 kDa, Protein from Homo sapiens)
- Cyclin-dependent kinase inhibitor 1b (22 kDa, Protein from Homo sapiens)
- Ubiquitin-conjugating enzyme e2 l3 (17 kDa, Protein from Homo sapiens)
- 5-azanylpentan-2-one (101 Da, Ligand)
- Polyubiquitin-c (8 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Nedd8-cul1-rbx1-skp1-skp2-ckshs1-cyclin a-cdk2-p27-ube2l3~ub~arih1. transition state 1 composite map. (300 kDa, Complex)
- Cyclin-dependent kinase 2 (34 kDa, Protein from Homo sapiens)
- Cullin-1, e3 ubiquitin-protein ligase rbx1 (Complex from Homo sapiens)
- S-phase kinase-associated protein 1 (18 kDa, Protein from Homo sapiens)
Horn-Ghetko D, Krist DT, Prabu JR, Baek K, Mulder MPC, Klugel M, Scott DC, Ovaa H, Kleiger G, Schulman BA
Nature (2021) 590 pp. 671-676 [ DOI: doi:10.1038/s41586-021-03197-9 Pubmed: 33536622 ]