Resolution: 3.34 Å
EM Method: Single-particle
Fitted PDBs: 7n4u
Q-score: 0.467
Long T, Liu Y, Qin Y, DeBose-Boyd RA, Li X
Sci Adv (2021) 7 [ DOI: doi:10.1126/sciadv.abh3997 Pubmed: 34407950 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- (2r)-2,5,7,8-tetramethyl-2-[(4r,8r)-4,8,12-trimethyltridecyl]chroman-6-ol (430 Da, Ligand)
- Npc1l1 apo state (Cell from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- (2s)-3-(hexadecanoyloxy)-2-[(9z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate (760 Da, Ligand)
- Isoform 2 of npc1-like intracellular cholesterol transporter 1 (145 kDa, Protein from Homo sapiens)
Structure of cholesterol-bound human NPC1L1
Resolution: 3.58 Å
EM Method: Single-particle
Fitted PDBs: 7n4v
Q-score: 0.453
Long T, Liu Y, Qin Y, DeBose-Boyd RA, Li X
Sci Adv (2021) 7 [ DOI: doi:10.1126/sciadv.abh3997 Pubmed: 34407950 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Human npc1l1 supplemented with cholesterol (Cellular component from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- (2s)-3-(hexadecanoyloxy)-2-[(9z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate (760 Da, Ligand)
- Isoform 2 of npc1-like intracellular cholesterol transporter 1 (145 kDa, Protein from Homo sapiens)
Structure of human NPC1L1 mutant-W347R
Resolution: 3.33 Å
EM Method: Single-particle
Fitted PDBs: 7n4x
Q-score: 0.525
Long T, Liu Y, Qin Y, DeBose-Boyd RA, Li X
Sci Adv (2021) 7 [ DOI: doi:10.1126/sciadv.abh3997 Pubmed: 34407950 ]
- Cholesterol (386 Da, Ligand)
- Isoform 2 of npc1-like intracellular cholesterol transporter 1 (145 kDa, Protein from Homo sapiens)
- Human npc1l1 mutant-w347r (Cellular component from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Cryo_EM structure of delta N-NPC1L1-CLR
Resolution: 2.69 Å
EM Method: Single-particle
Fitted PDBs: 7dfw
Q-score: 0.584
Hu M, Yang F, Huang Y, You X, Liu D, Sun S, Sui SF
Sci Adv (2021) 7 [ Pubmed: 34272236 DOI: doi:10.1126/sciadv.abg3188 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- O-[(r)-{[(2r)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-l-serine (792 Da, Ligand)
- Npc1-like intracellular cholesterol transporter 1 (141 kDa, Protein from Homo sapiens)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
- Complex of delta n-npc1l1 with clr (Complex from Homo sapiens)
Cryo_EM structure of delta N-NPC1L1-EZE
Resolution: 3.58 Å
EM Method: Single-particle
Fitted PDBs: 7dfz
Q-score: 0.481
Hu M, Yang F, Huang Y, You X, Liu D, Sun S, Sui SF
Sci Adv (2021) 7 [ DOI: doi:10.1126/sciadv.abg3188 Pubmed: 34272236 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Npc1-like intracellular cholesterol transporter 1 (139 kDa, Protein from Homo sapiens)
- Delta n-hnpc1l1-eze (Complex from Homo sapiens)
- (3~{r},4~{s})-1-(4-fluorophenyl)-3-[(3~{s})-3-(4-fluorophenyl)-3-oxidanyl-propyl]-4-(4-hydroxyphenyl)azetidin-2-one (409 Da, Ligand)
- Cholesterol (386 Da, Ligand)
Resolution: 3.03 Å
EM Method: Single-particle
Fitted PDBs: 7df8
Q-score: 0.54
Hu M, Yang F, Huang Y, You X, Liu D, Sun S, Sui SF
Sci Adv (2021) 7 [ Pubmed: 34272236 DOI: doi:10.1126/sciadv.abg3188 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Npc1-like intracellular cholesterol transporter 1 (139 kDa, Protein from Homo sapiens)
- Full length npc1l1 (Cell from Homo sapiens)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
Structure of NPC1-like intracellular cholesterol transporter 1 (NPC1L1)
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6v3f
Q-score: 0.455
Huang CS, Yu X, Fordstrom P, Choi K, Chung BC, Roh SH, Chiu W, Zhou M, Min X, Wang Z
Sci Adv (2020) 6 pp. eabb1989- [ DOI: doi:10.1126/sciadv.abb1989 ]
- Cholesterol (386 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Npc1-like intracellular cholesterol transporter 1 (npc1l1) (Cellular component from Rattus norvegicus)
- Npc1-like intracellular cholesterol transporter 1 (147 kDa, Protein from Rattus norvegicus)
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 6v3h
Q-score: 0.5
Huang CS, Yu X, Fordstrom P, Choi K, Chung BC, Roh SH, Chiu W, Zhou M, Min X, Wang Z
Sci Adv (2020) 6 pp. eabb1989- [ DOI: doi:10.1126/sciadv.abb1989 ]
- Npc1-like intracellular cholesterol transporter 1 (147 kDa, Protein from Rattus norvegicus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Npc1-like intracellular cholesterol transporter 1 (npc1l1) in complex with an inhibitor (Complex from Rattus norvegicus)
- 4-[(2s,3r)-3-[(3s)-3-(4-fluorophenyl)-3-hydroxypropyl]-1-(4-{3-[(methylsulfonyl)amino]prop-1-yn-1-yl}phenyl)-4-oxoazetidin-2-yl]phenyl beta-d-glucopyranosiduronic acid (698 Da, Ligand)
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6w5r
Q-score: 0.417
Qian H, Wu X, Du X, Yao X, Zhao X, Lee J, Yang H, Yan N
Cell (2020) 182 pp. 98-111.e18 [ DOI: doi:10.1016/j.cell.2020.05.020 Pubmed: 32544384 ]
- Cholesterol (386 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Npc1 nanodisc (150 kDa, Complex from Homo sapiens)
- Npc intracellular cholesterol transporter 1 (146 kDa, Protein from Homo sapiens)