Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 8e5k
Q-score: 0.228
Molodtsov V, Wang C, Firlar E, Kaelber JT, Ebright RH
Nature (2023) 614 pp. 367-374 [ Pubmed: 36697824 DOI: doi:10.1038/s41586-022-05658-1 ]
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Nt dna (18 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Transcription termination/antitermination protein nusg (20 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- T dna (18 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Escherichia coli rho-dependent transcription pre-termination complex containing 21 nt long rna spacer, mg-adp-bef3, and nusg, tec part (Complex from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Rna with 21 nt long spacer (11 kDa, RNA from synthetic construct)
Resolution: 4.4 Å
EM Method: Single-particle
Fitted PDBs: 8e5o
Q-score: 0.151
Molodtsov V, Wang C, Firlar E, Kaelber JT, Ebright RH
Nature (2023) 614 pp. 367-374 [ Pubmed: 36697824 DOI: doi:10.1038/s41586-022-05658-1 ]
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Nt dna (18 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Transcription termination/antitermination protein nusg (20 kDa, Protein from Escherichia coli)
- Escherichia coli rho-dependent transcription pre-termination complex containing 24 nt long rna spacer, mg-adp-bef3, and nusg; tec part (Complex from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- T dna (18 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Rna with 24 nt long spacer (12 kDa, RNA from synthetic construct)
Resolution: 4.27 Å
EM Method: Single-particle
Fitted PDBs: 8e6x
Q-score: 0.264
Molodtsov V, Wang C, Firlar E, Kaelber JT, Ebright RH
Nature (2023) 614 pp. 367-374 [ Pubmed: 36697824 DOI: doi:10.1038/s41586-022-05658-1 ]
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Escherichia coli rho-dependent transcription pre-termination complex containing 18 nt long rna spacer, lambda-tr1 rut rna, mg-adp-bef3, and nusg; tec part (Complex from Escherichia coli)
- Nt dna (18 kDa, DNA from synthetic construct)
- Rna with 18 nt long spacer (11 kDa, RNA from synthetic construct)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Transcription termination/antitermination protein nusg (20 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- T dna (18 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 8e6z
Q-score: 0.273
Molodtsov V, Wang C, Firlar E, Kaelber JT, Ebright RH
Nature (2023) 614 pp. 367-374 [ Pubmed: 36697824 DOI: doi:10.1038/s41586-022-05658-1 ]
- Magnesium ion (24 Da, Ligand)
- Transcription termination/antitermination protein nusg (20 kDa, Protein from Escherichia coli)
- Rna with 18 nt long spacer (11 kDa, RNA from synthetic construct)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Nt dna (18 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- T dna (18 kDa, DNA from synthetic construct)
- Escherichia coli rho-dependent transcription pre-termination complex containing 18 nt long rna spacer, dc75 rut mimic rna, mg-adp-bef3, and nusg; tec part (Complex from Escherichia coli)
Structure of yeast RNA Polymerase III Pre-Termination Complex (PTC)
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7z1l
Q-score: 0.538
Girbig M, Xie J, Grotsch H, Libri D, Porrua O, Muller CW
Cell Rep (2022) 40 pp. 111316-111316 [ DOI: doi:10.1016/j.celrep.2022.111316 Pubmed: 36070694 ]
- Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (25 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (8 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc8 (24 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (17 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc3 (74 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc7 (27 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc1 (162 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc10 (12 kDa, Protein from Saccharomyces cerevisiae W303)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase iii subunit rpc9 (18 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc6 (36 kDa, Protein from Saccharomyces cerevisiae W303)
- (3r,5s,7r,8r,9s,10s,12s,13r,14s,17r)-10,13-dimethyl-17-[(2r)-pentan-2-yl]-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1h-cyclopenta[a]phenanthrene-3,7,12-triol (378 Da, Ligand)
- Dna-directed rna polymerase iii subunit rpc2 (129 kDa, Protein from Saccharomyces cerevisiae W303)
- Yeast rna polymerase iii ptc (730 kDa, Complex from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc4 (46 kDa, Protein from Saccharomyces cerevisiae W303)
- Rna (7 kDa, RNA from synthetic construct)
- Nt-dna (13 kDa, DNA from synthetic construct)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (16 kDa, Protein from Saccharomyces cerevisiae W303)
- T-dna (13 kDa, DNA from synthetic construct)
- Dna-directed rna polymerases i and iii subunit rpac1 (37 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerases i and iii subunit rpac2 (16 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc5 (32 kDa, Protein from Saccharomyces cerevisiae W303)
Structure of yeast RNA Polymerase III Elongation Complex (EC)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7z1m
Q-score: 0.466
Girbig M, Xie J, Grotsch H, Libri D, Porrua O, Muller CW
Cell Rep (2022) 40 pp. 111316-111316 [ Pubmed: 36070694 DOI: doi:10.1016/j.celrep.2022.111316 ]
- Dna-directed rna polymerase iii subunit rpc9 (18 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc2 (129 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (16 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc10 (12 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (25 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerases i and iii subunit rpac2 (16 kDa, Protein from Saccharomyces cerevisiae W303)
- (3r,5s,7r,8r,9s,10s,12s,13r,14s,17r)-10,13-dimethyl-17-[(2r)-pentan-2-yl]-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1h-cyclopenta[a]phenanthrene-3,7,12-triol (378 Da, Ligand)
- Dna-directed rna polymerase iii subunit rpc8 (24 kDa, Protein from Saccharomyces cerevisiae W303)
- Nt-dna (13 kDa, Macromolecule from synthetic construct)
- Dna-directed rna polymerase iii subunit rpc3 (74 kDa, Protein from Saccharomyces cerevisiae W303)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase iii subunit rpc1 (162 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc6 (36 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (17 kDa, Protein from Saccharomyces cerevisiae W303)
- Yeast rna polymerase iii ec (730 kDa, Complex from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerases i and iii subunit rpac1 (37 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc5 (32 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc7 (27 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Saccharomyces cerevisiae W303)
- T-dna (13 kDa, Macromolecule from synthetic construct)
- Dna-directed rna polymerase iii subunit rpc4 (46 kDa, Protein from Saccharomyces cerevisiae W303)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (8 kDa, Protein from Saccharomyces cerevisiae W303)
- Rna (6 kDa, RNA from synthetic construct)
Structure of yeast RNA Polymerase III Delta C53-C37-C11
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7z1n
Q-score: 0.405
Girbig M, Xie J, Grotsch H, Libri D, Porrua O, Muller CW
Cell Rep (2022) 40 pp. 111316-111316 [ Pubmed: 36070694 DOI: doi:10.1016/j.celrep.2022.111316 ]
- Dna-directed rna polymerase iii subunit rpc9 (18 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc2 (129 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (16 kDa, Protein from Saccharomyces cerevisiae W303)
- Nt-dna (13 kDa, DNA from synthetic construct)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (25 kDa, Protein from Saccharomyces cerevisiae W303)
- Rna (7 kDa, RNA from synthetic construct)
- Dna-directed rna polymerases i and iii subunit rpac2 (16 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc8 (24 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc3 (74 kDa, Protein from Saccharomyces cerevisiae W303)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase iii subunit rpc1 (162 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc6 (36 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (17 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerases i and iii subunit rpac1 (37 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc7 (27 kDa, Protein from Saccharomyces cerevisiae W303)
- T-dna (13 kDa, DNA from synthetic construct)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Saccharomyces cerevisiae W303)
- Chapso (631 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (8 kDa, Protein from Saccharomyces cerevisiae W303)
- Yeast rna polymerase iii delta c53-c37-c11 (635 kDa, Complex from Saccharomyces cerevisiae W303)
Structure of yeast RNA Polymerase III PTC + NTPs
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7z1o
Q-score: 0.534
Girbig M, Xie J, Grotsch H, Libri D, Porrua O, Muller CW
Cell Rep (2022) 40 pp. 111316-111316 [ DOI: doi:10.1016/j.celrep.2022.111316 Pubmed: 36070694 ]
- Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (25 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (8 kDa, Protein from Saccharomyces cerevisiae W303)
- Chapso (631 Da, Ligand)
- Dna-directed rna polymerase iii subunit rpc8 (24 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (17 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc3 (74 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc7 (27 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc1 (162 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc10 (12 kDa, Protein from Saccharomyces cerevisiae W303)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase iii subunit rpc9 (18 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc6 (36 kDa, Protein from Saccharomyces cerevisiae W303)
- Yeast rna polymerase iii ptc + ntps (730 kDa, Complex from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc2 (129 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc4 (46 kDa, Protein from Saccharomyces cerevisiae W303)
- Nt-dna (13 kDa, DNA from synthetic construct)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (16 kDa, Protein from Saccharomyces cerevisiae W303)
- T-dna (13 kDa, DNA from synthetic construct)
- Rna (6 kDa, RNA from synthetic construct)
- Dna-directed rna polymerases i and iii subunit rpac1 (37 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerases i and iii subunit rpac2 (16 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc5 (32 kDa, Protein from Saccharomyces cerevisiae W303)
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 7py0
Q-score: 0.185
Zhu C, Guo X, Dumas P, Takacs M, Abdelkareem M, Vanden Broeck A, Saint-Andre C, Papai G, Crucifix C, Ortiz J, Weixlbaumer A
Nat Commun (2022) 13 pp. 1546-1546 [ Pubmed: 35318334 DOI: doi:10.1038/s41467-022-29148-0 ]
- Magnesium ion (24 Da, Ligand)
- Transcription termination/antitermination protein nusg (20 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Ntdna (12 kDa, DNA from Escherichia coli)
- Rna (4 kDa, RNA from Escherichia coli)
- Nusg elongation complex in more-swiveled conformation (Complex from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Tdna (11 kDa, DNA from Escherichia coli)
CryoEM structure of E.coli RNA polymerase elongation complex bound to NusG (the consensus NusG-EC)
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7py1
Q-score: 0.343
Zhu C, Guo X, Dumas P, Takacs M, Abdelkareem M, Vanden Broeck A, Saint-Andre C, Papai G, Crucifix C, Ortiz J, Weixlbaumer A
Nat Commun (2022) 13 pp. 1546-1546 [ Pubmed: 35318334 DOI: doi:10.1038/s41467-022-29148-0 ]
- Ntdna (12 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Tdna (11 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Transcription termination/antitermination protein nusg (20 kDa, Protein from Escherichia coli)
- Rna (4 kDa, RNA from Escherichia coli)
- Nusg elongation complex (consensus) (Complex from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)