Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7zrd
Q-score: 0.504
Silberberg JM, Stock C, Hielkema L, Corey RA, Rheinberger J, Wunnicke D, Dubach VRA, Stansfeld PJ, Hanelt I, Paulino C
eLife (2022) 11 [ Pubmed: 36255052 DOI: doi:10.7554/eLife.80988 ]
- Potassium-transporting atpase kdpf subunit (3 kDa, Protein from Escherichia coli)
- Potassium-transporting atpase atp-binding subunit (72 kDa, Protein from Escherichia coli)
- Potassium ion (39 Da, Ligand)
- Cardiolipin (1 kDa, Ligand)
- Potassium-transporting atpase kdpc subunit (20 kDa, Protein from Escherichia coli)
- Potassium-transporting atpase potassium-binding subunit (59 kDa, Protein from Escherichia coli)
- Kdpfabc (157 kDa, Complex from Escherichia coli)
- Vanadate ion (114 Da, Ligand)
Cryo-EM map of the WT KdpFABC complex in the E1-P tight conformation, under turnover conditions
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7zre
Q-score: 0.487
Silberberg JM, Stock C, Hielkema L, Corey RA, Rheinberger J, Wunnicke D, Dubach VRA, Stansfeld PJ, Hanelt I, Paulino C
eLife (2022) 11 [ Pubmed: 36255052 DOI: doi:10.7554/eLife.80988 ]
- Potassium ion (39 Da, Ligand)
- Cardiolipin (1 kDa, Ligand)
- Potassium-transporting atpase kdpc subunit (20 kDa, Protein from Escherichia coli)
- Potassium-transporting atpase atp-binding subunit (72 kDa, Protein from Escherichia coli)
- Potassium-transporting atpase kdpf subunit (2 kDa, Protein from Escherichia coli)
- Potassium-transporting atpase potassium-binding subunit (59 kDa, Protein from Escherichia coli)
- Kdpfabc (157 kDa, Complex from Escherichia coli)
Cryo-EM map of the WT KdpFABC complex in the E1_ATPearly conformation, under turnover conditions
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7zrg
Q-score: 0.465
Silberberg JM, Stock C, Hielkema L, Corey RA, Rheinberger J, Wunnicke D, Dubach VRA, Stansfeld PJ, Hanelt I, Paulino C
eLife (2022) 11 [ Pubmed: 36255052 DOI: doi:10.7554/eLife.80988 ]
- Potassium-transporting atpase kdpf subunit (2 kDa, Protein from Escherichia coli)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Kdpfabc (157 kDa, Complex from Escherichia coli)
- Potassium-transporting atpase kdpc subunit (20 kDa, Protein from Escherichia coli)
- Potassium-transporting atpase atp-binding subunit (72 kDa, Protein from Escherichia coli)
- Cardiolipin (1 kDa, Ligand)
- Potassium ion (39 Da, Ligand)
- Potassium-transporting atpase potassium-binding subunit (59 kDa, Protein from Escherichia coli)
Cryo-EM structure of the KdpFABC complex in a nucleotide-free E1 conformation loaded with K+
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7zrh
Q-score: 0.485
Silberberg JM, Stock C, Hielkema L, Corey RA, Rheinberger J, Wunnicke D, Dubach VRA, Stansfeld PJ, Hanelt I, Paulino C
eLife (2022) 11 [ Pubmed: 36255052 DOI: doi:10.7554/eLife.80988 ]
- Potassium ion (39 Da, Ligand)
- Cardiolipin (1 kDa, Ligand)
- Potassium-transporting atpase kdpc subunit (20 kDa, Protein from Escherichia coli)
- Potassium-transporting atpase kdpf subunit (2 kDa, Protein from Escherichia coli)
- Potassium-transporting atpase potassium-binding subunit (59 kDa, Protein from Escherichia coli)
- Kdpfabc (157 kDa, Complex from Escherichia coli)
- Potassium-transporting atpase atp-binding subunit (72 kDa, Protein from Escherichia coli)
Cryo-EM structure of the KdpFABC complex in a nucleotide-free E1 conformation loaded with K+
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7zri
Q-score: 0.457
Silberberg JM, Stock C, Hielkema L, Corey RA, Rheinberger J, Wunnicke D, Dubach VRA, Stansfeld PJ, Hanelt I, Paulino C
eLife (2022) 11 [ Pubmed: 36255052 DOI: doi:10.7554/eLife.80988 ]
- Potassium ion (39 Da, Ligand)
- Cardiolipin (1 kDa, Ligand)
- Potassium-transporting atpase kdpc subunit (20 kDa, Protein from Escherichia coli)
- Potassium-transporting atpase kdpf subunit (2 kDa, Protein from Escherichia coli)
- Potassium-transporting atpase potassium-binding subunit (59 kDa, Protein from Escherichia coli)
- Kdpfabc (157 kDa, Complex from Escherichia coli)
- Potassium-transporting atpase atp-binding subunit (72 kDa, Protein from Escherichia coli)
Cryo-EM structure of the KdpFABC complex in a nucleotide-free E1 conformation loaded with K+
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7zrj
Q-score: 0.429
Silberberg JM, Stock C, Hielkema L, Corey RA, Rheinberger J, Wunnicke D, Dubach VRA, Stansfeld PJ, Hanelt I, Paulino C
eLife (2022) 11 [ Pubmed: 36255052 DOI: doi:10.7554/eLife.80988 ]
- Potassium ion (39 Da, Ligand)
- Cardiolipin (1 kDa, Ligand)
- Potassium-transporting atpase kdpc subunit (20 kDa, Protein from Escherichia coli)
- Potassium-transporting atpase kdpf subunit (2 kDa, Protein from Escherichia coli)
- Potassium-transporting atpase potassium-binding subunit (59 kDa, Protein from Escherichia coli)
- Kdpfabc (157 kDa, Complex from Escherichia coli)
- Potassium-transporting atpase atp-binding subunit (72 kDa, Protein from Escherichia coli)
Cryo-EM map of the WT KdpFABC complex in the E1-P_ADP conformation, under turnover conditions
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7zrk
Q-score: 0.529
Silberberg JM, Stock C, Hielkema L, Corey RA, Rheinberger J, Wunnicke D, Dubach VRA, Stansfeld PJ, Hanelt I, Paulino C
eLife (2022) 11 [ Pubmed: 36255052 DOI: doi:10.7554/eLife.80988 ]
- Potassium-transporting atpase kdpf subunit (2 kDa, Protein from Escherichia coli)
- Potassium-transporting atpase atp-binding subunit (72 kDa, Protein from Escherichia coli)
- Kdpfabc (157 kDa, Complex from Escherichia coli)
- Potassium-transporting atpase kdpc subunit (20 kDa, Protein from Escherichia coli)
- Cardiolipin (1 kDa, Ligand)
- Potassium ion (39 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Potassium-transporting atpase potassium-binding subunit (59 kDa, Protein from Escherichia coli)
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 7zrl
Q-score: 0.42
Silberberg JM, Stock C, Hielkema L, Corey RA, Rheinberger J, Wunnicke D, Dubach VRA, Stansfeld PJ, Hanelt I, Paulino C
eLife (2022) 11 [ DOI: doi:10.7554/eLife.80988 Pubmed: 36255052 ]
- Potassium-transporting atpase atp-binding subunit (72 kDa, Protein from Escherichia coli K-12)
- Kdpfabc (157 kDa, Complex from Escherichia coli K-12)
- Potassium-transporting atpase potassium-binding subunit (59 kDa, Protein from Escherichia coli K-12)
- Potassium-transporting atpase kdpc subunit (20 kDa, Protein from Escherichia coli K-12)
- Potassium-transporting atpase kdpf subunit (2 kDa, Protein from Escherichia coli K-12)
- Potassium ion (39 Da, Ligand)
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7zrm
Q-score: 0.446
Silberberg JM, Stock C, Hielkema L, Corey RA, Rheinberger J, Wunnicke D, Dubach VRA, Stansfeld PJ, Hanelt I, Paulino C
eLife (2022) 11 [ Pubmed: 36255052 DOI: doi:10.7554/eLife.80988 ]
- Potassium-transporting atpase atp-binding subunit (72 kDa, Protein from Escherichia coli)
- Potassium ion (39 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Potassium-transporting atpase kdpc subunit (20 kDa, Protein from Escherichia coli)
- Potassium-transporting atpase kdpf subunit (2 kDa, Protein from Escherichia coli)
- Potassium-transporting atpase potassium-binding subunit (59 kDa, Protein from Escherichia coli)
- Kdpfabc (157 kDa, Complex from Escherichia coli)
Cryo-EM structure of the KdpFABC complex in an E1-ATP conformation loaded with K+
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7nnl
Q-score: 0.532
Silberberg JM, Corey RA, Hielkema L, Stock C, Stansfeld PJ, Paulino C, Hanelt I
Nat Commun (2021) 12 pp. 5098-5098 [ DOI: doi:10.1038/s41467-021-25242-x Pubmed: 34429416 ]
- Potassium-transporting atpase atp-binding subunit (72 kDa, Protein from Escherichia coli)
- Potassium-transporting atpase kdpf subunit (2 kDa, Protein from Escherichia coli)
- Kdpfabc (157 kDa, Complex from Escherichia coli)
- Potassium-transporting atpase kdpc subunit (20 kDa, Protein from Escherichia coli)
- Cardiolipin (1 kDa, Ligand)
- Potassium ion (39 Da, Ligand)
- Phosphomethylphosphonic acid adenylate ester (505 Da, Ligand)
- Potassium-transporting atpase potassium-binding subunit (59 kDa, Protein from Escherichia coli)