Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7zta
Q-score: 0.565
Leroy EC, Perry TN, Renault TT, Innis CA
Nat Chem Biol (2023) 19 pp. 1091-1096 [ DOI: doi:10.1038/s41589-023-01343-0 Pubmed: 37322159 ]
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s3 (23 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s5 (16 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal subunit (Complex from Escherichia coli K-12)
- 30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s11 (12 kDa, Protein from Escherichia coli K-12)
- Trna-gln (e site) (Complex from Escherichia coli K-12)
- 50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli K-12)
- Maaapqk nascent peptide (716 Da, Protein from Escherichia coli K-12)
- 50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l28 (8 kDa, Protein from Escherichia coli K-12)
- Cysteine (121 Da, Ligand)
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli K-12)
- 5s ribosomal rna (38 kDa, RNA from Escherichia coli K-12)
- 50s ribosomal protein l17 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l23 (10 kDa, Protein from Escherichia coli K-12)
- Structure of an escherichia coli 70s ribosome stalled by tetracenomycin x during translation of an maaapqk(c) peptide (Complex from Escherichia coli K-12)
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli K-12)
- Potassium ion (39 Da, Ligand)
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli K-12)
- Mrna (Complex from Escherichia coli K-12)
- Mrna (4 kDa, RNA from Escherichia coli K-12)
- Zinc ion (65 Da, Ligand)
- Tetracenomycin x (486 Da, Ligand)
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal subunit (Complex from Escherichia coli K-12)
- Trna-cys (a site) (Complex from Escherichia coli K-12)
- 50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s19 (9 kDa, Protein from Escherichia coli K-12)
- 16s ribosomal rna (497 kDa, RNA from Escherichia coli K-12)
- 50s ribosomal protein l33 (5 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s21 (6 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli K-12)
- Water (18 Da, Ligand)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli K-12)
- Trna-cys (23 kDa, RNA from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s18 (7 kDa, Protein from Escherichia coli K-12)
- Trna-lys (24 kDa, RNA from Escherichia coli K-12)
- Trna-gln (24 kDa, RNA from Escherichia coli K-12)
- 30s ribosomal protein s2 (24 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s7 (16 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli K-12)
- Maaapqk-trna-lys (p site) (Complex)
- Glutamine (146 Da, Ligand)
- 50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli K-12)
- 23s ribosomal rna (941 kDa, RNA from Escherichia coli K-12)
- 30s ribosomal protein s13 (12 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s6, fully modified isoform (12 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli K-12)
ARE-ABCF VmlR2 bound to a 70S ribosome
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8buu
Q-score: 0.514
Obana N, Takada H, Crowe-McAuliffe C, Iwamoto M, Egorov AA, Wu KJY, Chiba S, Murina V, Paternoga H, Tresco BIC, Nomura N, Myers AG, Atkinson GC, Wilson DN, Hauryliuk V
Nucleic Acids Res (2023) 51 pp. 4536-4554 [ DOI: doi:10.1093/nar/gkad193 Pubmed: 36951104 ]
- 30s ribosomal protein s15 (10 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l21 (11 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- Potassium ion (39 Da, Ligand)
- 30s ribosomal protein s19 (10 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l5 (20 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l17 (13 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 30s ribosomal protein s10 (11 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l24 (11 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l33 1 (5 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 23s rrna (949 kDa, RNA from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l22 (12 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 30s ribosomal protein s4 (22 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l18 (12 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- 70s ribosome with vmlr2 bound in the e site, a distorted trna in the p site, and a substoichiometric distorted trna in the a site (2 MDa, Complex from Neobacillus vireti)
- 50s ribosomal protein l4 (22 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l30 (6 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- Magnesium ion (24 Da, Ligand)
- 50s ribosomal protein l23 (10 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 30s ribosomal protein s2 (28 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 16s rrna (503 kDa, RNA from Bacillus subtilis subsp. subtilis str. 168)
- 30s ribosomal protein s12 (15 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- Expz (72 kDa, Protein from Neobacillus vireti)
- 50s ribosomal protein l3 (22 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l2 (30 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- Trna-fmet (24 kDa, RNA from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l36 (4 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l29 (7 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l16 (16 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 5s rrna (38 kDa, RNA from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l14 (13 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 30s ribosomal protein s14 (7 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 30s ribosomal protein s3 (24 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l13 (16 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l19 (13 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 30s ribosomal protein s5 (17 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 30s ribosomal protein s8 (14 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 30s ribosomal protein s18 (8 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 30s ribosomal protein s7 (17 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l15 (15 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 30s ribosomal protein s9 (14 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein s6 (11 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 30s ribosomal protein s17 (10 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- Mrna (4 kDa, RNA from Bacillus subtilis subsp. subtilis str. 168)
- 30s ribosomal protein s20 (9 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 30s ribosomal protein s13 (13 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l27 (10 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l35 (7 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l6 (19 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l32 (6 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l20 (13 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l34 (5 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 30s ribosomal protein s16 (10 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 30s ribosomal protein s11 (13 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l28 (6 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
Human mitochondrial small subunit assembly intermediate (State A)
Resolution: 2.66 Å
EM Method: Single-particle
Fitted PDBs: 8csp
Q-score: 0.548
Harper NJ, Burnside C, Klinge S
Nature (2023) 614 pp. 175-181 [ DOI: doi:10.1038/s41586-022-05621-0 Pubmed: 36482135 ]
- 28s ribosomal protein s34, mitochondrial (25 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s27, mitochondrial (47 kDa, Protein from Homo sapiens)
- Nitric oxide-associated protein 1 (78 kDa, Protein from Homo sapiens)
- 12s mitochondrial rrna (306 kDa, RNA from Homo sapiens)
- 28s ribosomal protein s9, mitochondrial (45 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s31, mitochondrial (45 kDa, Protein from Homo sapiens)
- Malonyl-coa-acyl carrier protein transacylase, mitochondrial (43 kDa, Protein from Homo sapiens)
- Human mitochondrial small subunit assembly intermediate, state a (1 MDa, Complex from Homo sapiens)
- Gtpase era, mitochondrial (48 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s12, mitochondrial (15 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s6, mitochondrial (14 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s26, mitochondrial (24 kDa, Protein from Homo sapiens)
- Pentatricopeptide repeat domain-containing protein 3, mitochondrial (78 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s29, mitochondrial (45 kDa, Protein from Homo sapiens)
- S-adenosylmethionine (398 Da, Ligand)
- 28s ribosomal protein s23, mitochondrial (21 kDa, Protein from Homo sapiens)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Dimethyladenosine transferase 1, mitochondrial (39 kDa, Protein from Homo sapiens)
- Potassium ion (39 Da, Ligand)
- 28s ribosomal protein s10, mitochondrial (23 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s17, mitochondrial (14 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Methyltransferase-like protein 17, mitochondrial (50 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s33, mitochondrial (12 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s25, mitochondrial (20 kDa, Protein from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- 28s ribosomal protein s5, mitochondrial (48 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s2, mitochondrial (33 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s24, mitochondrial (19 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s35, mitochondrial (36 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- 28s ribosomal protein s28, mitochondrial (20 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s7, mitochondrial (28 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s16, mitochondrial (15 kDa, Protein from Homo sapiens)
- Iron/sulfur cluster (351 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- 28s ribosomal protein s15, mitochondrial (29 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s22, mitochondrial (41 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s14, mitochondrial (15 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s18b, mitochondrial (29 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s18c, mitochondrial (15 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s21, mitochondrial (10 kDa, Protein from Homo sapiens)
Human mitochondrial small subunit assembly intermediate (State B)
Resolution: 2.54 Å
EM Method: Single-particle
Fitted PDBs: 8csq
Q-score: 0.566
Harper NJ, Burnside C, Klinge S
Nature (2023) 614 pp. 175-181 [ DOI: doi:10.1038/s41586-022-05621-0 Pubmed: 36482135 ]
- 28s ribosomal protein s34, mitochondrial (25 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s27, mitochondrial (47 kDa, Protein from Homo sapiens)
- 12s mitochondrial rrna (306 kDa, RNA from Homo sapiens)
- 28s ribosomal protein s9, mitochondrial (45 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s31, mitochondrial (45 kDa, Protein from Homo sapiens)
- Malonyl-coa-acyl carrier protein transacylase, mitochondrial (43 kDa, Protein from Homo sapiens)
- Gtpase era, mitochondrial (48 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s21, mitochondrial (10 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s12, mitochondrial (15 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s6, mitochondrial (14 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s26, mitochondrial (24 kDa, Protein from Homo sapiens)
- Pentatricopeptide repeat domain-containing protein 3, mitochondrial (78 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s29, mitochondrial (45 kDa, Protein from Homo sapiens)
- S-adenosylmethionine (398 Da, Ligand)
- 28s ribosomal protein s23, mitochondrial (21 kDa, Protein from Homo sapiens)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Dimethyladenosine transferase 1, mitochondrial (39 kDa, Protein from Homo sapiens)
- Potassium ion (39 Da, Ligand)
- 28s ribosomal protein s10, mitochondrial (23 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s17, mitochondrial (14 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Methyltransferase-like protein 17, mitochondrial (50 kDa, Protein from Homo sapiens)
- Aurora kinase a-interacting protein (22 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s33, mitochondrial (12 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s25, mitochondrial (20 kDa, Protein from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- 28s ribosomal protein s5, mitochondrial (48 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s2, mitochondrial (33 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s24, mitochondrial (19 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s35, mitochondrial (36 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- 28s ribosomal protein s28, mitochondrial (20 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s7, mitochondrial (28 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s16, mitochondrial (15 kDa, Protein from Homo sapiens)
- Iron/sulfur cluster (351 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- 28s ribosomal protein s15, mitochondrial (29 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s22, mitochondrial (41 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s14, mitochondrial (15 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s18b, mitochondrial (29 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s18c, mitochondrial (15 kDa, Protein from Homo sapiens)
- Human mitochondrial small subunit assembly intermediate, state b (1 MDa, Complex from Homo sapiens)
Human mitochondrial small subunit assembly intermediate (State C)
Resolution: 2.54 Å
EM Method: Single-particle
Fitted PDBs: 8csr
Q-score: 0.563
Harper NJ, Burnside C, Klinge S
Nature (2023) 614 pp. 175-181 [ DOI: doi:10.1038/s41586-022-05621-0 Pubmed: 36482135 ]
- 28s ribosomal protein s24, mitochondrial (19 kDa, Protein from human)
- Putative ribosome-binding factor a, mitochondrial (38 kDa, Protein from human)
- Aurora kinase a-interacting protein (22 kDa, Protein from human)
- 28s ribosomal protein s2, mitochondrial (33 kDa, Protein from human)
- 28s ribosomal protein s7, mitochondrial (28 kDa, Protein from human)
- Malonyl-coa-acyl carrier protein transacylase, mitochondrial (43 kDa, Protein from human)
- 28s ribosomal protein s25, mitochondrial (20 kDa, Protein from human)
- 28s ribosomal protein s33, mitochondrial (12 kDa, Protein from human)
- 28s ribosomal protein s18b, mitochondrial (29 kDa, Protein from human)
- 28s ribosomal protein s12, mitochondrial (15 kDa, Protein from human)
- 28s ribosomal protein s10, mitochondrial (23 kDa, Protein from human)
- Nicotinamide-adenine-dinucleotide (663 Da, Ligand)
- Methyltransferase-like protein 17, mitochondrial (50 kDa, Protein from human)
- 28s ribosomal protein s11, mitochondrial (20 kDa, Protein from human)
- 28s ribosomal protein s15, mitochondrial (29 kDa, Protein from human)
- S-adenosylmethionine (398 Da, Ligand)
- Pentatricopeptide repeat domain-containing protein 3, mitochondrial (78 kDa, Protein from human)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Potassium ion (39 Da, Ligand)
- 28s ribosomal protein s22, mitochondrial (41 kDa, Protein from human)
- 28s ribosomal protein s21, mitochondrial (10 kDa, Protein from human)
- Zinc ion (65 Da, Ligand)
- 28s ribosomal protein s34, mitochondrial (25 kDa, Protein from human)
- 28s ribosomal protein s9, mitochondrial (45 kDa, Protein from human)
- 12s mitochondrial rrna (306 kDa, RNA from human)
- 28s ribosomal protein s29, mitochondrial (45 kDa, Protein from human)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- 28s ribosomal protein s17, mitochondrial (14 kDa, Protein from human)
- 28s ribosomal protein s26, mitochondrial (24 kDa, Protein from human)
- 28s ribosomal protein s18c, mitochondrial (15 kDa, Protein from human)
- Water (18 Da, Ligand)
- 28s ribosomal protein s35, mitochondrial (36 kDa, Protein from human)
- 28s ribosomal protein s16, mitochondrial (15 kDa, Protein from human)
- 28s ribosomal protein s27, mitochondrial (47 kDa, Protein from human)
- Magnesium ion (24 Da, Ligand)
- 28s ribosomal protein s14, mitochondrial (15 kDa, Protein from human)
- Iron/sulfur cluster (351 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Human mitochondrial small subunit assembly intermediate, state c (1 MDa, Complex from Homo sapiens)
- 28s ribosomal protein s23, mitochondrial (21 kDa, Protein from human)
- 28s ribosomal protein s31, mitochondrial (45 kDa, Protein from human)
- 28s ribosomal protein s6, mitochondrial (14 kDa, Protein from human)
- 28s ribosomal protein s28, mitochondrial (20 kDa, Protein from human)
- Dimethyladenosine transferase 1, mitochondrial (39 kDa, Protein from human)
- 28s ribosomal protein s5, mitochondrial (48 kDa, Protein from human)
Human mitochondrial small subunit assembly intermediate (State D)
Resolution: 2.36 Å
EM Method: Single-particle
Fitted PDBs: 8css
Q-score: 0.576
Harper NJ, Burnside C, Klinge S
Nature (2023) 614 pp. 175-181 [ Pubmed: 36482135 DOI: doi:10.1038/s41586-022-05621-0 ]
- Potassium ion (39 Da, Ligand)
- S-adenosylmethionine (398 Da, Ligand)
- 28s ribosomal protein s27, mitochondrial (47 kDa, Protein from human)
- Malonyl-coa-acyl carrier protein transacylase, mitochondrial (43 kDa, Protein from human)
- 28s ribosomal protein s31, mitochondrial (45 kDa, Protein from human)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- 28s ribosomal protein s26, mitochondrial (24 kDa, Protein from human)
- 28s ribosomal protein s35, mitochondrial (36 kDa, Protein from human)
- Putative ribosome-binding factor a, mitochondrial (38 kDa, Protein from human)
- Magnesium ion (24 Da, Ligand)
- Pentatricopeptide repeat domain-containing protein 3, mitochondrial (78 kDa, Protein from human)
- 28s ribosomal protein s21, mitochondrial (10 kDa, Protein from human)
- 28s ribosomal protein s6, mitochondrial (14 kDa, Protein from human)
- 28s ribosomal protein s18c, mitochondrial (15 kDa, Protein from human)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- 28s ribosomal protein s33, mitochondrial (12 kDa, Protein from human)
- 28s ribosomal protein s12, mitochondrial (15 kDa, Protein from human)
- Methyltransferase-like protein 17, mitochondrial (50 kDa, Protein from human)
- 28s ribosomal protein s18b, mitochondrial (29 kDa, Protein from human)
- Nicotinamide-adenine-dinucleotide (663 Da, Ligand)
- 28s ribosomal protein s29, mitochondrial (45 kDa, Protein from human)
- 28s ribosomal protein s9, mitochondrial (45 kDa, Protein from human)
- Iron/sulfur cluster (351 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- 28s ribosomal protein s17, mitochondrial (14 kDa, Protein from human)
- 28s ribosomal protein s5, mitochondrial (48 kDa, Protein from human)
- 28s ribosomal protein s7, mitochondrial (28 kDa, Protein from human)
- 28s ribosomal protein s2, mitochondrial (33 kDa, Protein from human)
- 28s ribosomal protein s10, mitochondrial (23 kDa, Protein from human)
- 28s ribosomal protein s22, mitochondrial (41 kDa, Protein from human)
- 28s ribosomal protein s28, mitochondrial (20 kDa, Protein from human)
- 12s mitochondrial rrna (306 kDa, RNA from human)
- 28s ribosomal protein s14, mitochondrial (15 kDa, Protein from human)
- Zinc ion (65 Da, Ligand)
- 28s ribosomal protein s11, mitochondrial (20 kDa, Protein from human)
- 28s ribosomal protein s34, mitochondrial (25 kDa, Protein from human)
- 28s ribosomal protein s16, mitochondrial (15 kDa, Protein from human)
- 28s ribosomal protein s25, mitochondrial (20 kDa, Protein from human)
- Water (18 Da, Ligand)
- Aurora kinase a-interacting protein (22 kDa, Protein from human)
- 28s ribosomal protein s24, mitochondrial (19 kDa, Protein from human)
- 28s ribosomal protein s15, mitochondrial (29 kDa, Protein from human)
- 28s ribosomal protein s23, mitochondrial (21 kDa, Protein from human)
- Human mitochondrial small subunit assembly intermediate, state d (1 MDa, Complex from Homo sapiens)
Human mitochondrial small subunit assembly intermediate (State E)
Resolution: 2.85 Å
EM Method: Single-particle
Fitted PDBs: 8cst
Q-score: 0.555
Harper NJ, Burnside C, Klinge S
Nature (2023) 614 pp. 175-181 [ DOI: doi:10.1038/s41586-022-05621-0 Pubmed: 36482135 ]
- 28s ribosomal protein s24, mitochondrial (19 kDa, Protein from human)
- Putative ribosome-binding factor a, mitochondrial (38 kDa, Protein from human)
- Aurora kinase a-interacting protein (22 kDa, Protein from human)
- 28s ribosomal protein s2, mitochondrial (33 kDa, Protein from human)
- 28s ribosomal protein s7, mitochondrial (28 kDa, Protein from human)
- 28s ribosomal protein s25, mitochondrial (20 kDa, Protein from human)
- 28s ribosomal protein s33, mitochondrial (12 kDa, Protein from human)
- 28s ribosomal protein s18b, mitochondrial (29 kDa, Protein from human)
- 28s ribosomal protein s12, mitochondrial (15 kDa, Protein from human)
- 28s ribosomal protein s10, mitochondrial (23 kDa, Protein from human)
- Nicotinamide-adenine-dinucleotide (663 Da, Ligand)
- Methyltransferase-like protein 17, mitochondrial (50 kDa, Protein from human)
- 28s ribosomal protein s11, mitochondrial (20 kDa, Protein from human)
- 28s ribosomal protein s15, mitochondrial (29 kDa, Protein from human)
- S-adenosylmethionine (398 Da, Ligand)
- Pentatricopeptide repeat domain-containing protein 3, mitochondrial (78 kDa, Protein from human)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Potassium ion (39 Da, Ligand)
- 28s ribosomal protein s22, mitochondrial (41 kDa, Protein from human)
- Human mitochondrial small subunit assembly intermediate, state e (1 MDa, Complex from Homo sapiens)
- 28s ribosomal protein s21, mitochondrial (10 kDa, Protein from human)
- Zinc ion (65 Da, Ligand)
- 28s ribosomal protein s34, mitochondrial (25 kDa, Protein from human)
- 12s mitochondrial rrna (306 kDa, RNA from human)
- 28s ribosomal protein s9, mitochondrial (45 kDa, Protein from human)
- 28s ribosomal protein s29, mitochondrial (45 kDa, Protein from human)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- 28s ribosomal protein s17, mitochondrial (14 kDa, Protein from human)
- 28s ribosomal protein s26, mitochondrial (24 kDa, Protein from human)
- 28s ribosomal protein s18c, mitochondrial (15 kDa, Protein from human)
- 28s ribosomal protein s16, mitochondrial (15 kDa, Protein from human)
- 28s ribosomal protein s27, mitochondrial (47 kDa, Protein from human)
- Magnesium ion (24 Da, Ligand)
- 28s ribosomal protein s14, mitochondrial (15 kDa, Protein from human)
- Iron/sulfur cluster (351 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- 28s ribosomal protein s23, mitochondrial (21 kDa, Protein from human)
- 28s ribosomal protein s31, mitochondrial (45 kDa, Protein from human)
- 28s ribosomal protein s6, mitochondrial (14 kDa, Protein from human)
- 28s ribosomal protein s28, mitochondrial (20 kDa, Protein from human)
- 28s ribosomal protein s35, mitochondrial (36 kDa, Protein from human)
- 28s ribosomal protein s5, mitochondrial (48 kDa, Protein from human)
Human mitochondrial small subunit assembly intermediate (State C*)
Resolution: 3.03 Å
EM Method: Single-particle
Fitted PDBs: 8csu
Q-score: 0.519
Harper NJ, Burnside C, Klinge S
Nature (2023) 614 pp. 175-181 [ DOI: doi:10.1038/s41586-022-05621-0 Pubmed: 36482135 ]
- 28s ribosomal protein s24, mitochondrial (19 kDa, Protein from human)
- Putative ribosome-binding factor a, mitochondrial (38 kDa, Protein from human)
- Aurora kinase a-interacting protein (22 kDa, Protein from human)
- 28s ribosomal protein s2, mitochondrial (33 kDa, Protein from human)
- 28s ribosomal protein s7, mitochondrial (28 kDa, Protein from human)
- 28s ribosomal protein s25, mitochondrial (20 kDa, Protein from human)
- 28s ribosomal protein s33, mitochondrial (12 kDa, Protein from human)
- 28s ribosomal protein s18b, mitochondrial (29 kDa, Protein from human)
- 28s ribosomal protein s12, mitochondrial (15 kDa, Protein from human)
- 28s ribosomal protein s10, mitochondrial (23 kDa, Protein from human)
- Nicotinamide-adenine-dinucleotide (663 Da, Ligand)
- Methyltransferase-like protein 17, mitochondrial (50 kDa, Protein from human)
- 28s ribosomal protein s11, mitochondrial (20 kDa, Protein from human)
- 28s ribosomal protein s15, mitochondrial (29 kDa, Protein from human)
- S-adenosylmethionine (398 Da, Ligand)
- Pentatricopeptide repeat domain-containing protein 3, mitochondrial (78 kDa, Protein from human)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Potassium ion (39 Da, Ligand)
- 28s ribosomal protein s22, mitochondrial (41 kDa, Protein from human)
- 28s ribosomal protein s21, mitochondrial (10 kDa, Protein from human)
- Zinc ion (65 Da, Ligand)
- Human mitochondrial small subunit assembly intermediate, state c* (1 MDa, Complex from Homo sapiens)
- 28s ribosomal protein s34, mitochondrial (25 kDa, Protein from human)
- 28s ribosomal protein s9, mitochondrial (45 kDa, Protein from human)
- 12s mitochondrial rrna (306 kDa, RNA from human)
- 28s ribosomal protein s29, mitochondrial (45 kDa, Protein from human)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- 28s ribosomal protein s17, mitochondrial (14 kDa, Protein from human)
- 28s ribosomal protein s26, mitochondrial (24 kDa, Protein from human)
- 28s ribosomal protein s18c, mitochondrial (15 kDa, Protein from human)
- 28s ribosomal protein s35, mitochondrial (36 kDa, Protein from human)
- 28s ribosomal protein s16, mitochondrial (15 kDa, Protein from human)
- 28s ribosomal protein s27, mitochondrial (47 kDa, Protein from human)
- Magnesium ion (24 Da, Ligand)
- 28s ribosomal protein s14, mitochondrial (15 kDa, Protein from human)
- Iron/sulfur cluster (351 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- 28s ribosomal protein s23, mitochondrial (21 kDa, Protein from human)
- 28s ribosomal protein s31, mitochondrial (45 kDa, Protein from human)
- 28s ribosomal protein s6, mitochondrial (14 kDa, Protein from human)
- 28s ribosomal protein s28, mitochondrial (20 kDa, Protein from human)
- Dimethyladenosine transferase 1, mitochondrial (39 kDa, Protein from human)
- 28s ribosomal protein s5, mitochondrial (48 kDa, Protein from human)
Resolution: 2.79 Å
EM Method: Single-particle
Fitted PDBs: 7qca
Q-score: 0.538
McLaren M, Conners R, Isupov MN, Gil-Diez P, Gambelli L, Gold VAM, Walter A, Connell SR, Williams B, Daum B
Nat Microbiol (2023) [ DOI: doi:10.1038/s41564-023-01469-w ]
- 60s ribosomal protein l17 (19 kDa, Protein from Spraguea lophii 42 110)
- 60s ribosomal protein l39 (6 kDa, Protein from Spraguea lophii 42 110)
- El14 lm0 (14 kDa, Protein from Spraguea lophii 42 110)
- Ribosomal protein s21e (7 kDa, Protein from Spraguea lophii 42 110)
- 60s ribosomal protein l6 (20 kDa, Protein from Spraguea lophii 42 110)
- 40s ribosomal protein s3 (27 kDa, Protein from Spraguea lophii 42 110)
- 40s ribosomal protein s6 (26 kDa, Protein from Spraguea lophii 42 110)
- 60s ribosomal protein l8 (23 kDa, Protein from Spraguea lophii 42 110)
- 40s ribosomal protein s2 (27 kDa, Protein from Spraguea lophii 42 110)
- 60s ribosomal protein l36 (11 kDa, Protein from Spraguea lophii 42 110)
- 60s ribosomal protein l37a (10 kDa, Protein from Spraguea lophii 42 110)
- 60s ribosomal protein l7 (27 kDa, Protein from Spraguea lophii 42 110)
- 60s ribosomal protein l20 (20 kDa, Protein from Spraguea lophii 42 110)
- 40s ribosomal protein s13 (16 kDa, Protein from Spraguea lophii 42 110)
- Rna 5s (38 kDa, RNA from Spraguea lophii 42 110)
- 60s ribosomal protein l19 (19 kDa, Protein from Spraguea lophii 42 110)
- Es30 see (6 kDa, Protein from Spraguea lophii 42 110)
- 40s ribosomal protein s16 (16 kDa, Protein from Spraguea lophii 42 110)
- 60s ribosomal protein l13 (19 kDa, Protein from Spraguea lophii 42 110)
- 40s ribosomal protein s9 (21 kDa, Protein from Spraguea lophii 42 110)
- 40s ribosomal protein s10 (12 kDa, Protein from Spraguea lophii 42 110)
- Ul15 laa (16 kDa, Protein from Spraguea lophii 42 110)
- S60 ribosomal protein l10 (25 kDa, Protein from Spraguea lophii 42 110)
- 40s ribosomal protein s18 (18 kDa, Protein from Spraguea lophii 42 110)
- 40s ribosomal protein s15a (14 kDa, Protein from Spraguea lophii 42 110)
- 60s ribosomal protein l4 (37 kDa, Protein from Spraguea lophii 42 110)
- El37 ljj (10 kDa, Protein from Spraguea lophii 42 110)
- 60s ribosomal protein l3 (12 kDa, Protein from Spraguea lophii 42 110)
- 40s ribosomal protein s26 (11 kDa, Protein from Spraguea lophii 42 110)
- 60s ribosomal protein l23a (12 kDa, Protein from Spraguea lophii 42 110)
- Ribosomal protein l23 (15 kDa, Protein from Spraguea lophii 42 110)
- Guanine nucleotide binding protein beta subunit (36 kDa, Protein from Spraguea lophii 42 110)
- Ribosomal protein l15 (24 kDa, Protein from Spraguea lophii 42 110)
- 60s ribosomal protein l26 (15 kDa, Protein from Spraguea lophii 42 110)
- 40s ribosomal protein s19 (16 kDa, Protein from Spraguea lophii 42 110)
- 60s ribosomal protein l27 (18 kDa, Protein from Spraguea lophii 42 110)
- Ribosomal protein l13a (22 kDa, Protein from Spraguea lophii 42 110)
- Potassium ion (39 Da, Ligand)
- 40s ribosomal protein s0 (26 kDa, Protein from Spraguea lophii 42 110)
- 60s ribosomal protein l3 (44 kDa, Protein from Spraguea lophii 42 110)
- 40s ribosomal protein s12 (14 kDa, Protein from Spraguea lophii 42 110)
- 60s ribosomal protein l31 (12 kDa, Protein from Spraguea lophii 42 110)
- 60s ribosomal protein l44 (11 kDa, Protein from Spraguea lophii 42 110)
- Ribosomal protein s19 (18 kDa, Protein from Spraguea lophii 42 110)
- 60s ribosomal protein l5 (33 kDa, Protein from Spraguea lophii 42 110)
- 60s ribosomal protein l11 (19 kDa, Protein from Spraguea lophii 42 110)
- 40s ribosomal protein s11 (18 kDa, Protein from Spraguea lophii 42 110)
- Ribosome (Complex from Spraguea lophii 42 110)
- 60s ribosomal protein l35a (12 kDa, Protein from Spraguea lophii 42 110)
- 40s ribosomal protein s4 (31 kDa, Protein from Spraguea lophii 42 110)
- 60s ribosomal protein l22 (12 kDa, Protein from Spraguea lophii 42 110)
- Ribosomal protein l24e (15 kDa, Protein from Spraguea lophii 42 110)
- Ribosomal protein l34 (12 kDa, Protein from Spraguea lophii 42 110)
- 60s ribosomal protein l9 (21 kDa, Protein from Spraguea lophii 42 110)
- 40s ribosomal protein s8 (19 kDa, Protein from Spraguea lophii 42 110)
- Es1 sb0 (25 kDa, Protein from Spraguea lophii 42 110)
- Magnesium ion (24 Da, Ligand)
- 60s ribosomal protein l18 (21 kDa, Protein from Spraguea lophii 42 110)
- Es17 sr0 (14 kDa, Protein from Spraguea lophii 42 110)
- 40s ribosomal protein s7 (19 kDa, Protein from Spraguea lophii 42 110)
- 40s ribosomal protein s20 (13 kDa, Protein from Spraguea lophii 42 110)
- Rna ssu (444 kDa, RNA from Spraguea lophii 42 110)
- Us12 sx0 (15 kDa, Protein from Spraguea lophii 42 110)
- 40s ribosomal protein s29 (7 kDa, Protein from Spraguea lophii 42 110)
- 60s ribosomal protein l21 (18 kDa, Protein from Spraguea lophii 42 110)
- Es27 sbb (9 kDa, Protein from Spraguea lophii 42 110)
- 40s ribosomal protein s5 (21 kDa, Protein from Spraguea lophii 42 110)
- Rna 23s (849 kDa, RNA from Spraguea lophii 42 110)
- 60s ribosomal protein l8 (27 kDa, Protein from Spraguea lophii 42 110)
- 40s ribosomal protein s14 (14 kDa, Protein from Spraguea lophii 42 110)
- 40s ribosomal protein s25 (14 kDa, Protein from Spraguea lophii 42 110)
- Zinc ion (65 Da, Ligand)
- Ubiquitin (14 kDa, Protein from Spraguea lophii 42 110)
- Ribosomal l29 protein (14 kDa, Protein from Spraguea lophii 42 110)
- Es28 scc (7 kDa, Protein from Spraguea lophii 42 110)
- 60s ribosomal protein l32 (16 kDa, Protein from Spraguea lophii 42 110)
- Ubiquitin/40s ribosomal protein s27a fusion (16 kDa, Protein from Spraguea lophii 42 110)
- 40s ribosomal protein s24 (17 kDa, Protein from Spraguea lophii 42 110)
Translating 70S ribosome in the unrotated state (P and E, tRNAs)
Resolution: 1.55 Å
EM Method: Single-particle
Fitted PDBs: 8b0x
Q-score: 0.767
Fromm SA, O'Connor KM, Purdy M, Bhatt PR, Loughran G, Atkins JF, Jomaa A, Mattei S
Nat Commun (2023) 14 pp. 1095-1095 [ Pubmed: 36841832 DOI: doi:10.1038/s41467-023-36742-3 ]
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli B)
- Potassium ion (39 Da, Ligand)
- 30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli B)
- 50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli B)
- 23s rrna (948 kDa, RNA from Escherichia coli B)
- 50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli B)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli B)
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli B)
- 50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli B)
- 30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli B)
- 50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli B)
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli B)
- 50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli B)
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli B)
- 50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli B)
- 16s rrna (503 kDa, RNA from Escherichia coli B)
- 30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli B)
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli B)
- Magnesium ion (24 Da, Ligand)
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli B)
- 30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli B)
- 30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli B)
- 50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli B)
- 50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli B)
- 30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli B)
- Mrna (1 kDa, RNA from Escherichia coli B)
- 50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli B)
- 30s ribosomal protein s7 (20 kDa, Protein from Escherichia coli B)
- 50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli B)
- 50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli B)
- 5s rrna (38 kDa, RNA from Escherichia coli B)
- 50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli B)
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli B)
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli B)
- 50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli B)
- 30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli B)
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli B)
- 70s ribosome (2 MDa, Complex from Escherichia coli B)
- 50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli B)
- 50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli B)
- Zinc ion (65 Da, Ligand)
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli B)
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli B)
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli B)
- P-site trna (24 kDa, RNA from Escherichia coli B)
- 50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli B)
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli B)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli B)
- 50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli B)
- Water (18 Da, Ligand)
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli B)
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli B)
- 50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli B)
- 30s ribosomal protein s3 (26 kDa, Protein from Escherichia coli B)
- 50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli B)
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli B)
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli B)
- 30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli B)