Cryo-EM structure of Polytomella Complex-I (peripheral arm)
Resolution: 2.88 Å
EM Method: Single-particle
Fitted PDBs: 7arc
Q-score: 0.563
Klusch N, Senkler J, Yildiz O, Kuhlbrandt W, Braun HP
Plant Cell (2021) 33 pp. 2072-2091 [ Pubmed: 33768254 DOI: doi:10.1093/plcell/koab092 ]
- Psst (18 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- 13 kda (14 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- B14.5a (13 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- 24 kda (30 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- 51 kda (51 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Polytomella complex i - peripheral arm (Complex from Polytomella sp. Pringsheim 198.80)
- Nd9 (25 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Tyky (26 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- B13 (17 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Sdap2 (13 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Zinc ion (65 Da, Ligand)
- 39 kda (41 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Water (18 Da, Ligand)
- B14 (16 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Iron/sulfur cluster (351 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- 75 kda (77 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- B8 (10 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- B17.2 (18 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Flavin mononucleotide (456 Da, Ligand)
- Nd7 (45 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- 18 kda (20 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Cryo-EM structure of Polytomella Complex-I (complete composition)
Resolution: 3.11 Å
EM Method: Single-particle
Fitted PDBs: 7ard
Q-score: 0.557
Klusch N, Senkler J, Yildiz O, Kuhlbrandt W, Braun HP
Plant Cell (2021) 33 pp. 2072-2091 [ Pubmed: 33768254 DOI: doi:10.1093/plcell/koab092 ]
- Psst (18 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- 13 kda (14 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mwfe (8 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- B14.5a (13 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- (7s)-4-hydroxy-n,n,n-trimethyl-9-oxo-7-[(palmitoyloxy)methyl]-3,5,8-trioxa-4-phosphahexacosan-1-aminium 4-oxide (763 Da, Ligand)
- B9 (4 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- 24 kda (30 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- 51 kda (51 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Cardiolipin (1 kDa, Ligand)
- Nd9 (25 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Tyky (26 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- B14.7 (22 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Ca3 (24 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- B15 (16 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nd6 (15 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nd3 (17 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nd5 (58 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nd4l (13 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mnll (13 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Sdap1 (13 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nuop7 (13 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Ca2 (33 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Nuop4 (9 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nd1 (31 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Aggg (7 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Ashi (16 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Kfyi (12 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- 15 kda (8 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Pgiv (11 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- B12 (6 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nd2 (41 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- B13 (17 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- B18 (10 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Sdap2 (13 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Unknown (4 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Zinc ion (65 Da, Ligand)
- B14.5b (9 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- 39 kda (41 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Cryo-em structure of polytomella complex-i (complete composition) (Complex from Polytomella sp. Pringsheim 198.80)
- Water (18 Da, Ligand)
- Complex i-b22 (14 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- B14 (16 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- C1-fdx (23 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nuop5 (15 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Unknown (3 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- B16.6 (16 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Iron/sulfur cluster (351 Da, Ligand)
- Pdsw (18 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Cal (31 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Nd7 (45 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Phosphatidylethanolamine (734 Da, Ligand)
- 75 kda (77 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Esss (19 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- B8 (10 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- B17.2 (18 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nuop8 (15 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Flavin mononucleotide (456 Da, Ligand)
- Nd4 (48 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- 18 kda (20 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Plant mitochondrial respiratory complex I
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7a23
Q-score: 0.338
Soufari H, Parrot C, Kuhn L, Waltz F, Hashem Y
Nat Commun (2020) 11 pp. 5195-5195 [ DOI: doi:10.1038/s41467-020-18814-w Pubmed: 33060577 ]
- Acpm2 (14 kDa, Protein from Wild cabbage)
- Ca1 (30 kDa, Protein from Wild cabbage)
- B13 (19 kDa, Protein from Wild cabbage)
- P1 (11 kDa, Protein from Wild cabbage)
- 75kda (81 kDa, Protein from Wild cabbage)
- 13kda (12 kDa, Protein from Wild cabbage)
- Nad6m (23 kDa, Protein from Wild cabbage)
- B14 (15 kDa, Protein from Wild cabbage)
- Nad4lm (11 kDa, Protein from Wild cabbage)
- B15 (8 kDa, Protein from Wild cabbage)
- Nad9m (22 kDa, Protein from Wild cabbage)
- 20.9kda (11 kDa, Protein from Wild cabbage)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Ubiquinone-10 (863 Da, Ligand)
- Nad2m (55 kDa, Protein from Wild cabbage)
- Flavin mononucleotide (456 Da, Ligand)
- P2 (11 kDa, Protein from Wild cabbage)
- B8 (10 kDa, Protein from Wild cabbage)
- Cal1 (27 kDa, Protein from Wild cabbage)
- Nad5m (74 kDa, Protein from Wild cabbage)
- 39kda (43 kDa, Protein from Wild cabbage)
- 24kda (28 kDa, Protein from Wild cabbage)
- B14.5b (9 kDa, Protein from Wild cabbage)
- Mwfe (7 kDa, Protein from Wild cabbage)
- Nad4m (55 kDa, Protein from Wild cabbage)
- Nad3m (13 kDa, Protein from Wild cabbage)
- B22 (13 kDa, Protein from Wild cabbage)
- Tyky (25 kDa, Protein from Wild cabbage)
- B18 (11 kDa, Protein from Wild cabbage)
- Psst (24 kDa, Protein from Wild cabbage)
- 15kda (9 kDa, Protein from Wild cabbage)
- Unk1 (1 kDa, Protein from Wild cabbage)
- Mitochondrial respiratory complex i (Complex from Brassica oleracea)
- B12-1 (8 kDa, Protein from Wild cabbage)
- Phosphatidylinositol (887 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Nad1m (36 kDa, Protein from Wild cabbage)
- Unk2 (1 kDa, Protein from Wild cabbage)
- Bicarbonate ion (61 Da, Ligand)
- Pdsw (12 kDa, Protein from Wild cabbage)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Esss (12 kDa, Protein from Wild cabbage)
- Cardiolipin (1 kDa, Ligand)
- 51kda (53 kDa, Protein from Wild cabbage)
- B9 (6 kDa, Protein from Wild cabbage)
- 18kda (17 kDa, Protein from Wild cabbage)
- Aggg (7 kDa, Protein from Wild cabbage)
- (1s)-2-{[(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl stearate (720 Da, Ligand)
- B14.5a (15 kDa, Protein from Wild cabbage)
- Iron/sulfur cluster (351 Da, Ligand)
- Nad7m (45 kDa, Protein from Wild cabbage)
- Acpm1 (13 kDa, Protein from Wild cabbage)
- Pgiv (11 kDa, Protein from Wild cabbage)
- B17.2 (18 kDa, Protein from Wild cabbage)
- B16.6 (16 kDa, Protein from Wild cabbage)
Assembly intermediate of the plant mitochondrial complex I
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7a24
Q-score: 0.207
Soufari H, Parrot C, Kuhn L, Waltz F, Hashem Y
Nat Commun (2020) 11 pp. 5195-5195 [ DOI: doi:10.1038/s41467-020-18814-w Pubmed: 33060577 ]
- Nad6m (23 kDa, Protein from Brassica oleracea)
- 39kda (43 kDa, Protein from Brassica oleracea)
- B14 (15 kDa, Protein from Brassica oleracea)
- Cardiolipin (1 kDa, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Tyky (25 kDa, Protein from Brassica oleracea)
- 75kda (81 kDa, Protein from Brassica oleracea)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- 24kda (28 kDa, Protein from Brassica oleracea)
- B16.6 (16 kDa, Protein from Brassica oleracea)
- Cal1 (27 kDa, Protein from Brassica oleracea)
- Mwfe (7 kDa, Protein from Brassica oleracea)
- Nad4lm (11 kDa, Protein from Brassica oleracea)
- Pgiv (11 kDa, Protein from Brassica oleracea)
- Phosphatidylinositol (887 Da, Ligand)
- 13kda (12 kDa, Protein from Brassica oleracea)
- Nad2m (55 kDa, Protein from Brassica oleracea)
- Flavin mononucleotide (456 Da, Ligand)
- Unk1 (1 kDa, Protein from Brassica oleracea)
- Iron/sulfur cluster (351 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Gldh (68 kDa, Protein from Brassica oleracea)
- (1s)-2-{[(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl stearate (720 Da, Ligand)
- Nad7m (45 kDa, Protein from Brassica oleracea)
- 51kda (53 kDa, Protein from Brassica oleracea)
- B13 (19 kDa, Protein from Brassica oleracea)
- B17.2 (18 kDa, Protein from Brassica oleracea)
- Acpm1 (13 kDa, Protein from Brassica oleracea)
- P2 (11 kDa, Protein from Brassica oleracea)
- Nad1m (36 kDa, Protein from Brassica oleracea)
- Plant mitochondrial respiratory complex i assembly intermediate in presence of gldh (Complex from Brassica oleracea)
- 18kda (17 kDa, Protein from Brassica oleracea)
- Mnll (11 kDa, Protein from Brassica oleracea)
- Nad9m (22 kDa, Protein from Brassica oleracea)
- Psst (24 kDa, Protein from Brassica oleracea)
- B8 (10 kDa, Protein from Brassica oleracea)
- B14.5b (9 kDa, Protein from Brassica oleracea)
- 15kda (9 kDa, Protein from Brassica oleracea)
- B14.5a (15 kDa, Protein from Brassica oleracea)
- Nad3m (13 kDa, Protein from Brassica oleracea)
- B9 (6 kDa, Protein from Brassica oleracea)
- Bicarbonate ion (61 Da, Ligand)
- Ca1 (30 kDa, Protein from Brassica oleracea)
Complex I during turnover, open3
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 6zkf
Q-score: 0.564
Science (2020) 370 [ Pubmed: 32972993 DOI: doi:10.1126/science.abc4209 ]
- Nadh:ubiquinone oxidoreductase core subunit s1 (79 kDa, Protein from Sheep)
- Adenosine monophosphate (347 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 5 (68 kDa, Protein from Sheep)
- Cardiolipin (1 kDa, Ligand)
- Acyl carrier protein (17 kDa, Protein from Sheep)
- Nadh-ubiquinone oxidoreductase chain 2 (39 kDa, Protein from Sheep)
- Nadh:ubiquinone oxidoreductase subunit b7 (16 kDa, Protein from Sheep)
- Mitochondrial complex i, esss subunit (17 kDa, Protein from Sheep)
- Nadh-ubiquinone oxidoreductase chain 6 (19 kDa, Protein from Sheep)
- Nadh-ubiquinone oxidoreductase chain 4 (52 kDa, Protein from Sheep)
- Nadh:ubiquinone oxidoreductase subunit a9 (43 kDa, Protein from Sheep)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Mitochondrial complex i, b13 subunit (13 kDa, Protein from Sheep)
- Mitochondrial complex i, tyky subunit (24 kDa, Protein from Sheep)
- Nadh:ubiquinone oxidoreductase subunit a6 (16 kDa, Protein from Sheep)
- S-[2-({n-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate (568 Da, Ligand)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 1 (35 kDa, Protein from Sheep)
- 1,2-diacyl-sn-glycero-3-phosphoethanolamine (748 Da, Ligand)
- Mitochondrial complex i, 24 kda subunit (27 kDa, Protein from Sheep)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (21 kDa, Protein from Sheep)
- Nadh-ubiquinone oxidoreductase chain 3 (13 kDa, Protein from Sheep)
- Nadh-ubiquinone oxidoreductase chain 4l (10 kDa, Protein from Sheep)
- Mitochondrial complex i, pdsw subunit (20 kDa, Protein from Sheep)
- Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (50 kDa, Protein from Sheep)
- Flavin mononucleotide (456 Da, Ligand)
- Mitochondrial complex i, 49 kda subunit (52 kDa, Protein from Sheep)
- Potassium ion (39 Da, Ligand)
- Nadh:ubiquinone oxidoreductase subunit b9 (21 kDa, Protein from Sheep)
- Zinc ion (65 Da, Ligand)
- Nadh:ubiquinone oxidoreductase subunit s5 (12 kDa, Protein from Sheep)
- Nadh:ubiquinone oxidoreductase subunit b3 (11 kDa, Protein from Sheep)
- Mitochondrial complex i, psst subunit (24 kDa, Protein from Sheep)
- 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione (322 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 subunit c2 (14 kDa, Protein from Sheep)
- Mitochondrial complex i, b14.7 subunit (14 kDa, Protein from Sheep)
- Mitochondrial complex i, b16.6 subunit (16 kDa, Protein from Sheep)
- Nadh:ubiquinone oxidoreductase core subunit s3 (30 kDa, Protein from Sheep)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 (20 kDa, Protein from Sheep)
- 1,4-dihydronicotinamide adenine dinucleotide (665 Da, Ligand)
- Mitochondrial complex i, 13 kda subunit (13 kDa, Protein from Sheep)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (19 kDa, Protein from Sheep)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial (40 kDa, Protein from Sheep)
- Nadh:ubiquinone oxidoreductase subunit b5 (21 kDa, Protein from Sheep)
- Nadh:ubiquinone oxidoreductase subunit b2 (12 kDa, Protein from Sheep)
- Mitochondrial complex i, kfyi subunit (8 kDa, Protein from Sheep)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 (11 kDa, Protein from Sheep)
- Mitochondrial complex i, b17 subunit (15 kDa, Protein from Sheep)
- Mitochondrial complex i, mwfe subunit (8 kDa, Protein from Sheep)
- Mitochondrial complex i, b14.5a subunit (12 kDa, Protein from Sheep)
- Nadh:ubiquinone oxidoreductase subunit a3 (9 kDa, Protein from Sheep)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 (17 kDa, Protein from Sheep)
- Mitochondrial complex i, mnll subunit (7 kDa, Protein from Sheep)
- Complex i during turnover, open3 (1 MDa, Complex from Ovis aries)
- Myristic acid (228 Da, Ligand)
- Nadh:ubiquinone oxidoreductase subunit b4 (15 kDa, Protein from Sheep)
- Mitochondrial complex i, 10 kda subunit (11 kDa, Protein from Sheep)
- Iron/sulfur cluster (351 Da, Ligand)
Peripheral domain of open complex I during turnover
Resolution: 2.3 Å
EM Method: Single-particle
Fitted PDBs: 6zk9
Q-score: 0.66
Science (2020) 370 [ Pubmed: 32972993 DOI: doi:10.1126/science.abc4209 ]
- Nadh:ubiquinone oxidoreductase core subunit s1 (79 kDa, Protein from Sheep)
- Cardiolipin (1 kDa, Ligand)
- Acyl carrier protein (17 kDa, Protein from Sheep)
- Nadh:ubiquinone oxidoreductase subunit a9 (43 kDa, Protein from Sheep)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Mitochondrial complex i, b13 subunit (13 kDa, Protein from Sheep)
- Peripheral domain of open complex i during turnover (1 MDa, Complex from Ovis aries)
- Mitochondrial complex i, tyky subunit (24 kDa, Protein from Sheep)
- Nadh:ubiquinone oxidoreductase subunit a6 (16 kDa, Protein from Sheep)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- S-[2-({n-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate (568 Da, Ligand)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- 1,2-diacyl-sn-glycero-3-phosphoethanolamine (748 Da, Ligand)
- Mitochondrial complex i, 24 kda subunit (27 kDa, Protein from Sheep)
- Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (50 kDa, Protein from Sheep)
- Flavin mononucleotide (456 Da, Ligand)
- Mitochondrial complex i, 49 kda subunit (52 kDa, Protein from Sheep)
- Potassium ion (39 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Mitochondrial complex i, psst subunit (24 kDa, Protein from Sheep)
- Mitochondrial complex i, b16.6 subunit (16 kDa, Protein from Sheep)
- Water (18 Da, Ligand)
- Nadh:ubiquinone oxidoreductase core subunit s3 (30 kDa, Protein from Sheep)
- 1,4-dihydronicotinamide adenine dinucleotide (665 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (19 kDa, Protein from Sheep)
- Mitochondrial complex i, 13 kda subunit (13 kDa, Protein from Sheep)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 (11 kDa, Protein from Sheep)
- Mitochondrial complex i, b14.5a subunit (12 kDa, Protein from Sheep)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 (17 kDa, Protein from Sheep)
- Mitochondrial complex i, 10 kda subunit (11 kDa, Protein from Sheep)
- Iron/sulfur cluster (351 Da, Ligand)
Complex I during turnover, closed
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 6zkc
Q-score: 0.523
Science (2020) 370 [ Pubmed: 32972993 DOI: doi:10.1126/science.abc4209 ]
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial (40 kDa, Protein from Sheep)
- Potassium ion (39 Da, Ligand)
- Nadh:ubiquinone oxidoreductase subunit a6 (16 kDa, Protein from Sheep)
- Mitochondrial complex i, psst subunit (24 kDa, Protein from Sheep)
- Nadh:ubiquinone oxidoreductase subunit b3 (11 kDa, Protein from Sheep)
- Mitochondrial complex i, pdsw subunit (20 kDa, Protein from Sheep)
- Nadh:ubiquinone oxidoreductase subunit b5 (21 kDa, Protein from Sheep)
- Nadh:ubiquinone oxidoreductase subunit b9 (21 kDa, Protein from Sheep)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- 1,2-diacyl-sn-glycero-3-phosphoethanolamine (748 Da, Ligand)
- Adenosine monophosphate (347 Da, Ligand)
- Nadh:ubiquinone oxidoreductase core subunit s1 (79 kDa, Protein from Sheep)
- Mitochondrial complex i, mnll subunit (7 kDa, Protein from Sheep)
- Mitochondrial complex i, 13 kda subunit (13 kDa, Protein from Sheep)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (19 kDa, Protein from Sheep)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 (17 kDa, Protein from Sheep)
- Nadh:ubiquinone oxidoreductase subunit b7 (16 kDa, Protein from Sheep)
- 1,4-dihydronicotinamide adenine dinucleotide (665 Da, Ligand)
- Flavin mononucleotide (456 Da, Ligand)
- Acyl carrier protein (17 kDa, Protein from Sheep)
- Myristic acid (228 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 (11 kDa, Protein from Sheep)
- 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione (322 Da, Ligand)
- Mitochondrial complex i, esss subunit (17 kDa, Protein from Sheep)
- Nadh-ubiquinone oxidoreductase chain 2 (39 kDa, Protein from Sheep)
- Nadh-ubiquinone oxidoreductase chain 4l (10 kDa, Protein from Sheep)
- Nadh:ubiquinone oxidoreductase subunit s5 (12 kDa, Protein from Sheep)
- Mitochondrial complex i, b13 subunit (13 kDa, Protein from Sheep)
- Mitochondrial complex i, 24 kda subunit (27 kDa, Protein from Sheep)
- Nadh:ubiquinone oxidoreductase subunit a9 (43 kDa, Protein from Sheep)
- Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (50 kDa, Protein from Sheep)
- Nadh-ubiquinone oxidoreductase chain 5 (68 kDa, Protein from Sheep)
- S-[2-({n-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate (568 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 (20 kDa, Protein from Sheep)
- Mitochondrial complex i, b17 subunit (15 kDa, Protein from Sheep)
- Zinc ion (65 Da, Ligand)
- Complex i during turnover, closed (1 MDa, Complex from Ovis aries)
- Nadh:ubiquinone oxidoreductase subunit b2 (12 kDa, Protein from Sheep)
- Nadh-ubiquinone oxidoreductase chain 4 (52 kDa, Protein from Sheep)
- Nadh dehydrogenase [ubiquinone] 1 subunit c2 (14 kDa, Protein from Sheep)
- Mitochondrial complex i, mwfe subunit (8 kDa, Protein from Sheep)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Mitochondrial complex i, 49 kda subunit (52 kDa, Protein from Sheep)
- Nadh:ubiquinone oxidoreductase subunit b4 (15 kDa, Protein from Sheep)
- Mitochondrial complex i, b14.5a subunit (12 kDa, Protein from Sheep)
- Mitochondrial complex i, b16.6 subunit (16 kDa, Protein from Sheep)
- Cardiolipin (1 kDa, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
- Mitochondrial complex i, 10 kda subunit (11 kDa, Protein from Sheep)
- Mitochondrial complex i, tyky subunit (24 kDa, Protein from Sheep)
- Nadh-ubiquinone oxidoreductase chain 6 (19 kDa, Protein from Sheep)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (21 kDa, Protein from Sheep)
- Nadh:ubiquinone oxidoreductase core subunit s3 (30 kDa, Protein from Sheep)
- Nadh:ubiquinone oxidoreductase subunit a3 (9 kDa, Protein from Sheep)
- Nadh-ubiquinone oxidoreductase chain 3 (13 kDa, Protein from Sheep)
- Nadh-ubiquinone oxidoreductase chain 1 (35 kDa, Protein from Sheep)
- Mitochondrial complex i, b14.7 subunit (14 kDa, Protein from Sheep)
- Mitochondrial complex i, kfyi subunit (8 kDa, Protein from Sheep)
Complex I during turnover, open1
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 6zkd
Q-score: 0.579
Science (2020) 370 [ Pubmed: 32972993 DOI: doi:10.1126/science.abc4209 ]
- Nadh:ubiquinone oxidoreductase core subunit s1 (79 kDa, Protein from Sheep)
- Adenosine monophosphate (347 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 5 (68 kDa, Protein from Sheep)
- Cardiolipin (1 kDa, Ligand)
- Acyl carrier protein (17 kDa, Protein from Sheep)
- Complex i during turnover, open1 (1 MDa, Complex from Ovis aries)
- Nadh-ubiquinone oxidoreductase chain 2 (39 kDa, Protein from Sheep)
- Nadh:ubiquinone oxidoreductase subunit b7 (16 kDa, Protein from Sheep)
- Mitochondrial complex i, esss subunit (17 kDa, Protein from Sheep)
- Nadh-ubiquinone oxidoreductase chain 6 (19 kDa, Protein from Sheep)
- Nadh-ubiquinone oxidoreductase chain 4 (52 kDa, Protein from Sheep)
- Nadh:ubiquinone oxidoreductase subunit a9 (43 kDa, Protein from Sheep)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Mitochondrial complex i, b13 subunit (13 kDa, Protein from Sheep)
- Mitochondrial complex i, tyky subunit (24 kDa, Protein from Sheep)
- Nadh:ubiquinone oxidoreductase subunit a6 (16 kDa, Protein from Sheep)
- S-[2-({n-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate (568 Da, Ligand)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 1 (35 kDa, Protein from Sheep)
- 1,2-diacyl-sn-glycero-3-phosphoethanolamine (748 Da, Ligand)
- Mitochondrial complex i, 24 kda subunit (27 kDa, Protein from Sheep)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (21 kDa, Protein from Sheep)
- Nadh-ubiquinone oxidoreductase chain 3 (13 kDa, Protein from Sheep)
- Nadh-ubiquinone oxidoreductase chain 4l (10 kDa, Protein from Sheep)
- Mitochondrial complex i, pdsw subunit (20 kDa, Protein from Sheep)
- Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (50 kDa, Protein from Sheep)
- Flavin mononucleotide (456 Da, Ligand)
- Mitochondrial complex i, 49 kda subunit (52 kDa, Protein from Sheep)
- Potassium ion (39 Da, Ligand)
- Nadh:ubiquinone oxidoreductase subunit b9 (21 kDa, Protein from Sheep)
- Zinc ion (65 Da, Ligand)
- Nadh:ubiquinone oxidoreductase subunit s5 (12 kDa, Protein from Sheep)
- Nadh:ubiquinone oxidoreductase subunit b3 (11 kDa, Protein from Sheep)
- Mitochondrial complex i, psst subunit (24 kDa, Protein from Sheep)
- 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione (322 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 subunit c2 (14 kDa, Protein from Sheep)
- Mitochondrial complex i, b14.7 subunit (14 kDa, Protein from Sheep)
- Mitochondrial complex i, b16.6 subunit (16 kDa, Protein from Sheep)
- Nadh:ubiquinone oxidoreductase core subunit s3 (30 kDa, Protein from Sheep)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 (20 kDa, Protein from Sheep)
- 1,4-dihydronicotinamide adenine dinucleotide (665 Da, Ligand)
- Mitochondrial complex i, 13 kda subunit (13 kDa, Protein from Sheep)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (19 kDa, Protein from Sheep)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial (40 kDa, Protein from Sheep)
- Nadh:ubiquinone oxidoreductase subunit b5 (21 kDa, Protein from Sheep)
- Nadh:ubiquinone oxidoreductase subunit b2 (12 kDa, Protein from Sheep)
- Mitochondrial complex i, kfyi subunit (8 kDa, Protein from Sheep)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 (11 kDa, Protein from Sheep)
- Mitochondrial complex i, b17 subunit (15 kDa, Protein from Sheep)
- Mitochondrial complex i, mwfe subunit (8 kDa, Protein from Sheep)
- Mitochondrial complex i, b14.5a subunit (12 kDa, Protein from Sheep)
- Nadh:ubiquinone oxidoreductase subunit a3 (9 kDa, Protein from Sheep)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 (17 kDa, Protein from Sheep)
- Mitochondrial complex i, mnll subunit (7 kDa, Protein from Sheep)
- Myristic acid (228 Da, Ligand)
- Nadh:ubiquinone oxidoreductase subunit b4 (15 kDa, Protein from Sheep)
- Mitochondrial complex i, 10 kda subunit (11 kDa, Protein from Sheep)
- Iron/sulfur cluster (351 Da, Ligand)
Complex I during turnover, open2
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 6zke
Q-score: 0.598
Science (2020) 370 [ Pubmed: 32972993 DOI: doi:10.1126/science.abc4209 ]
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial (40 kDa, Protein from Sheep)
- Potassium ion (39 Da, Ligand)
- Nadh:ubiquinone oxidoreductase subunit a6 (16 kDa, Protein from Sheep)
- Mitochondrial complex i, psst subunit (24 kDa, Protein from Sheep)
- Nadh:ubiquinone oxidoreductase subunit b3 (11 kDa, Protein from Sheep)
- Mitochondrial complex i, pdsw subunit (20 kDa, Protein from Sheep)
- Nadh:ubiquinone oxidoreductase subunit b5 (21 kDa, Protein from Sheep)
- Nadh:ubiquinone oxidoreductase subunit b9 (21 kDa, Protein from Sheep)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- 1,2-diacyl-sn-glycero-3-phosphoethanolamine (748 Da, Ligand)
- Adenosine monophosphate (347 Da, Ligand)
- Nadh:ubiquinone oxidoreductase core subunit s1 (79 kDa, Protein from Sheep)
- Mitochondrial complex i, mnll subunit (7 kDa, Protein from Sheep)
- Mitochondrial complex i, 13 kda subunit (13 kDa, Protein from Sheep)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (19 kDa, Protein from Sheep)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 (17 kDa, Protein from Sheep)
- Nadh:ubiquinone oxidoreductase subunit b7 (16 kDa, Protein from Sheep)
- 1,4-dihydronicotinamide adenine dinucleotide (665 Da, Ligand)
- Flavin mononucleotide (456 Da, Ligand)
- Acyl carrier protein (17 kDa, Protein from Sheep)
- Myristic acid (228 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 (11 kDa, Protein from Sheep)
- 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione (322 Da, Ligand)
- Mitochondrial complex i, esss subunit (17 kDa, Protein from Sheep)
- Nadh-ubiquinone oxidoreductase chain 2 (39 kDa, Protein from Sheep)
- Nadh-ubiquinone oxidoreductase chain 4l (10 kDa, Protein from Sheep)
- Nadh:ubiquinone oxidoreductase subunit s5 (12 kDa, Protein from Sheep)
- Mitochondrial complex i, b13 subunit (13 kDa, Protein from Sheep)
- Mitochondrial complex i, 24 kda subunit (27 kDa, Protein from Sheep)
- Nadh:ubiquinone oxidoreductase subunit a9 (43 kDa, Protein from Sheep)
- Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (50 kDa, Protein from Sheep)
- Nadh-ubiquinone oxidoreductase chain 5 (68 kDa, Protein from Sheep)
- S-[2-({n-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate (568 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 (20 kDa, Protein from Sheep)
- Mitochondrial complex i, b17 subunit (15 kDa, Protein from Sheep)
- Zinc ion (65 Da, Ligand)
- Nadh:ubiquinone oxidoreductase subunit b2 (12 kDa, Protein from Sheep)
- Nadh-ubiquinone oxidoreductase chain 4 (52 kDa, Protein from Sheep)
- Nadh dehydrogenase [ubiquinone] 1 subunit c2 (14 kDa, Protein from Sheep)
- Mitochondrial complex i, mwfe subunit (8 kDa, Protein from Sheep)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Mitochondrial complex i, 49 kda subunit (52 kDa, Protein from Sheep)
- Nadh:ubiquinone oxidoreductase subunit b4 (15 kDa, Protein from Sheep)
- Mitochondrial complex i, b14.5a subunit (12 kDa, Protein from Sheep)
- Mitochondrial complex i, b16.6 subunit (16 kDa, Protein from Sheep)
- Cardiolipin (1 kDa, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
- Mitochondrial complex i, 10 kda subunit (11 kDa, Protein from Sheep)
- Mitochondrial complex i, tyky subunit (24 kDa, Protein from Sheep)
- Nadh-ubiquinone oxidoreductase chain 6 (19 kDa, Protein from Sheep)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (21 kDa, Protein from Sheep)
- Complex i during turnover, open2 (1 MDa, Complex from Ovis aries)
- Nadh:ubiquinone oxidoreductase core subunit s3 (30 kDa, Protein from Sheep)
- Nadh:ubiquinone oxidoreductase subunit a3 (9 kDa, Protein from Sheep)
- Nadh-ubiquinone oxidoreductase chain 3 (13 kDa, Protein from Sheep)
- Nadh-ubiquinone oxidoreductase chain 1 (35 kDa, Protein from Sheep)
- Mitochondrial complex i, b14.7 subunit (14 kDa, Protein from Sheep)
- Mitochondrial complex i, kfyi subunit (8 kDa, Protein from Sheep)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 6zkg
Q-score: 0.48
Science (2020) 370 [ Pubmed: 32972993 DOI: doi:10.1126/science.abc4209 ]
- Nadh:ubiquinone oxidoreductase core subunit s1 (79 kDa, Protein from Sheep)
- Adenosine monophosphate (347 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 5 (68 kDa, Protein from Sheep)
- Cardiolipin (1 kDa, Ligand)
- Acyl carrier protein (17 kDa, Protein from Sheep)
- Nadh-ubiquinone oxidoreductase chain 2 (39 kDa, Protein from Sheep)
- Nadh:ubiquinone oxidoreductase subunit b7 (16 kDa, Protein from Sheep)
- Mitochondrial complex i, esss subunit (17 kDa, Protein from Sheep)
- Nadh-ubiquinone oxidoreductase chain 6 (19 kDa, Protein from Sheep)
- Nadh-ubiquinone oxidoreductase chain 4 (52 kDa, Protein from Sheep)
- Nadh:ubiquinone oxidoreductase subunit a9 (43 kDa, Protein from Sheep)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Mitochondrial complex i, b13 subunit (13 kDa, Protein from Sheep)
- Mitochondrial complex i, tyky subunit (24 kDa, Protein from Sheep)
- Nadh:ubiquinone oxidoreductase subunit a6 (16 kDa, Protein from Sheep)
- S-[2-({n-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate (568 Da, Ligand)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 1 (35 kDa, Protein from Sheep)
- 1,2-diacyl-sn-glycero-3-phosphoethanolamine (748 Da, Ligand)
- Mitochondrial complex i, 24 kda subunit (27 kDa, Protein from Sheep)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (21 kDa, Protein from Sheep)
- Nadh-ubiquinone oxidoreductase chain 3 (13 kDa, Protein from Sheep)
- Nadh-ubiquinone oxidoreductase chain 4l (10 kDa, Protein from Sheep)
- Mitochondrial complex i, pdsw subunit (20 kDa, Protein from Sheep)
- Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (50 kDa, Protein from Sheep)
- Flavin mononucleotide (456 Da, Ligand)
- Mitochondrial complex i, 49 kda subunit (52 kDa, Protein from Sheep)
- Potassium ion (39 Da, Ligand)
- Nadh:ubiquinone oxidoreductase subunit b9 (21 kDa, Protein from Sheep)
- Zinc ion (65 Da, Ligand)
- Nadh:ubiquinone oxidoreductase subunit s5 (12 kDa, Protein from Sheep)
- Nadh:ubiquinone oxidoreductase subunit b3 (11 kDa, Protein from Sheep)
- Mitochondrial complex i, psst subunit (24 kDa, Protein from Sheep)
- Nadh dehydrogenase [ubiquinone] 1 subunit c2 (14 kDa, Protein from Sheep)
- Mitochondrial complex i, b14.7 subunit (14 kDa, Protein from Sheep)
- Mitochondrial complex i, b16.6 subunit (16 kDa, Protein from Sheep)
- Nadh:ubiquinone oxidoreductase core subunit s3 (30 kDa, Protein from Sheep)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 (20 kDa, Protein from Sheep)
- 1,4-dihydronicotinamide adenine dinucleotide (665 Da, Ligand)
- Mitochondrial complex i, 13 kda subunit (13 kDa, Protein from Sheep)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (19 kDa, Protein from Sheep)
- Complex i with nadh, closed (1 MDa, Complex from Ovis aries)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial (40 kDa, Protein from Sheep)
- Nadh:ubiquinone oxidoreductase subunit b5 (21 kDa, Protein from Sheep)
- Nadh:ubiquinone oxidoreductase subunit b2 (12 kDa, Protein from Sheep)
- Mitochondrial complex i, kfyi subunit (8 kDa, Protein from Sheep)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 (11 kDa, Protein from Sheep)
- Mitochondrial complex i, b17 subunit (15 kDa, Protein from Sheep)
- Mitochondrial complex i, mwfe subunit (8 kDa, Protein from Sheep)
- Mitochondrial complex i, b14.5a subunit (12 kDa, Protein from Sheep)
- Nadh:ubiquinone oxidoreductase subunit a3 (9 kDa, Protein from Sheep)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 (17 kDa, Protein from Sheep)
- Mitochondrial complex i, mnll subunit (7 kDa, Protein from Sheep)
- Myristic acid (228 Da, Ligand)
- Nadh:ubiquinone oxidoreductase subunit b4 (15 kDa, Protein from Sheep)
- Mitochondrial complex i, 10 kda subunit (11 kDa, Protein from Sheep)
- Iron/sulfur cluster (351 Da, Ligand)