Human spliceosomal PM5 C* complex
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8c6j
Q-score: 0.353
Dybkov O, Preussner M, El Ayoubi L, Feng VY, Harnisch C, Merz K, Leupold P, Yudichev P, Agafonov DE, Will CL, Girard C, Dienemann C, Urlaub H, Kastner B, Heyd F, Luehrmann R
Sci Adv (2023) 9 pp. eadf1785- [ DOI: doi:10.1126/sciadv.adf1785 Pubmed: 36867703 ]
- Peptidyl-prolyl cis-trans isomerase-like 3 (18 kDa, Protein from human)
- U5 snrna (36 kDa, RNA from human)
- Crooked neck-like protein 1 (83 kDa, Protein from human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from human)
- Rna-binding protein 8a (19 kDa, Protein from human)
- Pre-mrna-splicing factor isy1 homolog (33 kDa, Protein from human)
- Pre-mrna-processing factor 17 (65 kDa, Protein from human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Intron-binding protein aquarius (171 kDa, Protein from human)
- Pre-mrna-processing factor 19 (55 kDa, Protein from human)
- Protein mago nashi homolog (17 kDa, Protein from human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from human)
- Inositol hexakisphosphate (660 Da, Ligand)
- U6 snrna (34 kDa, RNA from human)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from human)
- Nitric oxide synthase-interacting protein (33 kDa, Protein from human)
- Pm5 5' exon (78 kDa, RNA from Homo sapiens)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from human)
- Pleiotropic regulator 1 (57 kDa, Protein from human)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from human)
- U2 snrna (60 kDa, RNA from human)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from human)
- Potassium ion (39 Da, Ligand)
- Pre-mrna-splicing factor cwc22 homolog (57 kDa, Protein from human)
- Zinc ion (65 Da, Ligand)
- Atp-dependent rna helicase dhx8 (139 kDa, Protein from human)
- Splicing factor cactin (88 kDa, Protein from human)
- Splicing regulator sde2 (49 kDa, Protein from human)
- Pre-mrna-splicing factor slu7 (68 kDa, Protein from human)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from human)
- Cell division cycle 5-like protein (92 kDa, Protein from human)
- Prkr-interacting protein 1 (21 kDa, Protein from human)
- Pre-mrna-splicing factor spf27 (26 kDa, Protein from human)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Human spliceosomal c* complex assembled on pm5 pre-mrna (Complex from Homo sapiens)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from human)
- Sting er exit protein (25 kDa, Protein from human)
- Protein fam32a (13 kDa, Protein from human)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from human)
- Magnesium ion (24 Da, Ligand)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from human)
- Probable atp-dependent rna helicase ddx41 (69 kDa, Protein from human)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from human)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from human)
- Snw domain-containing protein 1 (61 kDa, Protein from human)
- Protein bud31 homolog (17 kDa, Protein from human)
- Nf-kappa-b-activating protein (47 kDa, Protein from human)
- Peptidyl-prolyl cis-trans isomerase e (33 kDa, Protein from human)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from human)
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from human)
- Eukaryotic initiation factor 4a-iii (46 kDa, Protein from human)
- Splicing factor c9orf78 (33 kDa, Protein from human)
- Protein fam50a (40 kDa, Protein from human)
- Pm5 intron (74 kDa, RNA from Homo sapiens)
- Pre-mrna-splicing factor syf2 (28 kDa, Protein from human)
- Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from human)
- Splicing factor ess-2 homolog (52 kDa, Protein from human)
Resolution: 8.0 Å
EM Method: Single-particle
Fitted PDBs: 7abi
Q-score: 0.145
Townsend C, Leelaram MN, Agafonov DE, Dybkov O, Will CL, Bertram K, Urlaub H, Kastner B, Stark H, Luhrmann R
Science (2020) 370 [ DOI: doi:10.1126/science.abc3753 Pubmed: 33243851 ]
- U6 snrna (34 kDa, RNA from Human)
- Splicing factor 3b subunit 1 (146 kDa, Protein from Human)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Human)
- Splicing factor 3b subunit 6 (14 kDa, Protein from Human)
- Minx m3 pre-mrna (103 kDa, RNA from synthetic construct)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from Human)
- Pre-mrna-splicing factor 38a (37 kDa, Protein from Human)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Human)
- Rna-binding motif protein, x-linked 2 (37 kDa, Protein from Human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Dna/rna-binding protein kin17 (45 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Human)
- Splicing factor 3a subunit 2 (49 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Human)
- Splicing factor 3a subunit 3 (58 kDa, Protein from Human)
- Serine/arginine repetitive matrix protein 1 (102 kDa, Protein from Human)
- U5 snrna (36 kDa, RNA from Human)
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from Human)
- Zinc finger matrin-type protein 2 (23 kDa, Protein from Human)
- Magnesium ion (24 Da, Ligand)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Human)
- Smad nuclear-interacting protein 1 (45 kDa, Protein from Human)
- Protein bud31 homolog (17 kDa, Protein from Human)
- Beta-catenin-like protein 1 (65 kDa, Protein from Human)
- Inositol hexakisphosphate (660 Da, Ligand)
- Phd finger-like domain-containing protein 5a (12 kDa, Protein from Human)
- Splicing factor 3b subunit 5 (10 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Human)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Human)
- Snw domain-containing protein 1 (61 kDa, Protein from Human)
- U2 snrna (60 kDa, RNA from Human)
- Human pre-bact-2 spliceosome (Complex from Homo sapiens)
- Pleiotropic regulator 1 (57 kDa, Protein from Human)
- Cell division cycle 5-like protein (92 kDa, Protein from Human)
- Splicing factor 3b subunit 2 (100 kDa, Protein from Human)
- Minx m3 pre-mrna (Complex from synthetic construct)
- Splicing factor 3b subunit 4 (44 kDa, Protein from Human)
- Microfibrillar-associated protein 1 (52 kDa, Protein from Human)
- Ubiquitin-like protein 5 (8 kDa, Protein from Human)
- Peptidyl-prolyl cis-trans isomerase e (33 kDa, Protein from Human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Human)
- Pre-mrna-processing factor 17 (65 kDa, Protein from Human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Human)
- Splicing factor 3a subunit 1 (88 kDa, Protein from Human)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Human)
- Intron-binding protein aquarius (171 kDa, Protein from Human)
- Ring-type e3 ubiquitin-protein ligase ppil2 (58 kDa, Protein from Human)
- Splicing factor 3b subunit 3 (135 kDa, Protein from Human)
- Crooked neck-like protein 1 (100 kDa, Protein from Human)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from Human)
- Human pre-bact-2 spliceosome (Complex)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Human)
- Bud13 homolog (70 kDa, Protein from Human)
Resolution: 7.8 Å
EM Method: Single-particle
Fitted PDBs: 7abg
Q-score: 0.147
Townsend C, Leelaram MN, Agafonov DE, Dybkov O, Will CL, Bertram K, Urlaub H, Kastner B, Stark H, Luhrmann R
Science (2020) 370 [ DOI: doi:10.1126/science.abc3753 Pubmed: 33243851 ]
- Splicing factor 3b subunit 5 (10 kDa, Protein from Homo sapiens)
- U6 snrna-associated sm-like protein lsm3 (11 kDa, Protein from Homo sapiens)
- U6 snrna (34 kDa, RNA from Homo sapiens)
- Splicing factor 3b subunit 6 (14 kDa, Protein from Homo sapiens)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Homo sapiens)
- Ubiquitin-like protein 5 (8 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 2 (100 kDa, Protein from Homo sapiens)
- Zinc finger matrin-type protein 2 (23 kDa, Protein from Homo sapiens)
- U6 snrna-associated sm-like protein lsm6 (9 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Homo sapiens)
- Splicing factor 3a subunit 2 (49 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Homo sapiens)
- Phd finger-like domain-containing protein 5a (12 kDa, Protein from Homo sapiens)
- U6 snrna-associated sm-like protein lsm7 (11 kDa, Protein from Homo sapiens)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 4 (44 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from Homo sapiens)
- Pleiotropic regulator 1 (57 kDa, Protein from Homo sapiens)
- Inositol hexakisphosphate (660 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- U6 snrna-associated sm-like protein lsm5 (9 kDa, Protein from Homo sapiens)
- U2 snrna (60 kDa, RNA from Homo sapiens)
- Snw domain-containing protein 1 (61 kDa, Protein from Homo sapiens)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Homo sapiens)
- U6 snrna-associated sm-like protein lsm4 (15 kDa, Protein from Homo sapiens)
- U6 snrna-associated sm-like protein lsm2 (10 kDa, Protein from Homo sapiens)
- Splicing factor 3a subunit 1 (88 kDa, Protein from Homo sapiens)
- Microfibrillar-associated protein 1 (52 kDa, Protein from Homo sapiens)
- Serine/arginine-rich splicing factor 1 (27 kDa, Protein from Homo sapiens)
- Ww domain-binding protein 11 (70 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 3 (135 kDa, Protein from Homo sapiens)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Homo sapiens)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Homo sapiens)
- Minx m3 pre-mrna (73 kDa, RNA from synthetic construct)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Homo sapiens)
- Serine/arginine repetitive matrix protein 1 (102 kDa, Protein from Homo sapiens)
- Nuclear cap-binding protein subunit 1 (91 kDa, Protein from Homo sapiens)
- Transcription elongation regulator 1 (124 kDa, Protein from Homo sapiens)
- Splicing factor 3a subunit 3 (58 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Homo sapiens)
- Nuclear cap-binding protein subunit 2 (18 kDa, Protein from Homo sapiens)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Homo sapiens)
- Pre-bact-1 spliceosomal complex (Complex)
- Pre-bact-1 spliceosomal complex (Complex from Homo sapiens)
- 7-methyl-guanosine-5'-triphosphate-5'-guanosine (803 Da, Ligand)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Homo sapiens)
- U5 snrna (36 kDa, RNA from Homo sapiens)
- U6 snrna-associated sm-like protein lsm8 (10 kDa, Protein from Homo sapiens)
- Protein bud31 homolog (17 kDa, Protein from Homo sapiens)
- Cell division cycle 5-like protein (92 kDa, Protein from Homo sapiens)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Homo sapiens)
- Smad nuclear-interacting protein 1 (45 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 1 (146 kDa, Protein from Homo sapiens)
- Pre-mrna-splicing factor 38a (37 kDa, Protein from Homo sapiens)
Human pre-Bact-2 spliceosome core structure
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 7aav
Q-score: 0.393
Townsend C, Leelaram MN, Agafonov DE, Dybkov O, Will CL, Bertram K, Urlaub H, Kastner B, Stark H, Luhrmann R
Science (2020) 370 [ DOI: doi:10.1126/science.abc3753 Pubmed: 33243851 ]
- U2 snrna (60 kDa, RNA from Human)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Human)
- U5 snrna (36 kDa, RNA from Human)
- Pre-mrna-processing factor 17 (65 kDa, Protein from Human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Human)
- Pleiotropic regulator 1 (57 kDa, Protein from Human)
- Ubiquitin-like protein 5 (8 kDa, Protein from Human)
- Pre-bact-2 spliceosomal core structure (Complex)
- D-chiro inositol hexakisphosphate (660 Da, Ligand)
- Zinc finger matrin-type protein 2 (23 kDa, Protein from Human)
- Cell division cycle 5-like protein (92 kDa, Protein from Human)
- Minx m3 pre-mrna (73 kDa, RNA from synthetic construct)
- Snw domain-containing protein 1 (61 kDa, Protein from Human)
- Microfibrillar-associated protein 1 (52 kDa, Protein from Human)
- Protein bud31 homolog (17 kDa, Protein from Human)
- Magnesium ion (24 Da, Ligand)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from Human)
- Pre-bact-2 spliceosomal core structure (Complex from Homo sapiens)
- Pre-mrna-splicing factor 38a (37 kDa, Protein from Human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Human)
- Minx m3 pre-mrna (Complex from synthetic construct)
- U6 snrna (34 kDa, RNA from Human)
Human pre-Bact-1 spliceosome core structure
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7abf
Q-score: 0.374
Townsend C, Leelaram MN, Agafonov DE, Dybkov O, Will CL, Bertram K, Urlaub H, Kastner B, Stark H, Luhrmann R
Science (2020) 370 [ DOI: doi:10.1126/science.abc3753 Pubmed: 33243851 ]
- Inositol hexakisphosphate (660 Da, Ligand)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Human)
- Pleiotropic regulator 1 (57 kDa, Protein from Human)
- Minx m3 rna (Complex from synthetic construct)
- Pre-bact-1 spliceosomal complex (Complex from Homo sapiens)
- Ubiquitin-like protein 5 (8 kDa, Protein from Human)
- Ww domain-binding protein 11 (70 kDa, Protein from Human)
- Pre-bact-1 spliceosomal complex (Complex)
- Zinc finger matrin-type protein 2 (23 kDa, Protein from Human)
- Snw domain-containing protein 1 (61 kDa, Protein from Human)
- U5 small nuclear rna (36 kDa, RNA from Human)
- Microfibrillar-associated protein 1 (52 kDa, Protein from Human)
- Protein bud31 homolog (17 kDa, Protein from Human)
- Magnesium ion (24 Da, Ligand)
- Transcription elongation regulator 1 (121 kDa, Protein from Human)
- Pre-mrna-splicing factor 38a (37 kDa, Protein from Human)
- U6 small nuclear rna (34 kDa, RNA from Human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Human)
- Minx m3 rna (73 kDa, RNA from synthetic construct)