Structure of the human U5.U4/U6 tri-snRNP at 2.9A resolution.
Resolution: 2.92 Å
EM Method: Single-particle
Fitted PDBs: 6qw6
Q-score: 0.361
Charenton C, Wilkinson ME, Nagai K
Science (2019) 364 pp. 362-367 [ DOI: doi:10.1126/science.aax3289 Pubmed: 30975767 ]
- U6 snrna-associated sm-like protein lsm4 (15 kDa, Protein from Human)
- U6 snrna-associated sm-like protein lsm8 (10 kDa, Protein from Human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Human)
- U6 snrna-associated sm-like protein lsm6 (9 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Human)
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from Human)
- Magnesium ion (24 Da, Ligand)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Human)
- Nhp2-like protein 1 (14 kDa, Protein from Human)
- U6 snrna-associated sm-like protein lsm2 (10 kDa, Protein from Human)
- U4/u6 small nuclear ribonucleoprotein prp31 (55 kDa, Protein from Human)
- Rna-binding protein 42 (50 kDa, Protein from Human)
- Thioredoxin-like protein 4a (16 kDa, Protein from Human)
- Inositol hexakisphosphate (660 Da, Ligand)
- Probable atp-dependent rna helicase ddx23 (Complex from Homo sapiens)
- U6 snrna-associated sm-like protein lsm7 (11 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Human)
- U6 snrna-associated sm-like protein lsm5 (9 kDa, Protein from Human)
- Human u4/u6.u5 tri-snrnp (1 MDa, Complex)
- Probable atp-dependent rna helicase ddx23 (95 kDa, Protein from Homo sapiens)
- U4/u6.u5 tri-snrnp-associated protein 2 (64 kDa, Protein from Human)
- Zinc ion (65 Da, Ligand)
- U4/u6 small nuclear ribonucleoprotein prp3 (77 kDa, Protein from Human)
- Pre-mrna-processing-splicing factor 8 (271 kDa, Protein from Human)
- U4/u6.u5 tri-snrnp-associated protein 1 (90 kDa, Protein from Human)
- U5.u4/u6 tri-snrnp (Complex from Homo sapiens)
- U4/u6 small nuclear ribonucleoprotein prp4 (58 kDa, Protein from Human)
- U6 snrna (28 kDa, RNA from Human)
- 116 kda u5 small nuclear ribonucleoprotein component (95 kDa, Protein from Human)
- U5 snrna (37 kDa, RNA from Human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Human)
- U4 snrna (46 kDa, RNA from Human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Human)
- Pre-mrna-processing factor 6 (107 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Human)
- U6 snrna-associated sm-like protein lsm3 (11 kDa, Protein from Human)
- U4/u6.u5 small nuclear ribonucleoprotein 27 kda protein (18 kDa, Protein from Human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Human)
Structure of a human fully-assembled precatalytic spliceosome (pre-B complex).
Resolution: 3.28 Å
EM Method: Single-particle
Fitted PDBs: 6qx9
Q-score: 0.207
Charenton C, Wilkinson ME, Nagai K
Science (2019) 364 pp. 362-367 [ Pubmed: 30975767 DOI: doi:10.1126/science.aax3289 ]
- Pre-mrna-processing factor 6 (97 kDa, Protein from Human)
- Inositol hexakisphosphate (660 Da, Ligand)
- U4/u6.u5 small nuclear ribonucleoprotein 27 kda protein (18 kDa, Protein from Human)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Human)
- Nhp2-like protein 1 (14 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Human)
- 116 kda u5 small nuclear ribonucleoprotein component (95 kDa, Protein from Human)
- U4/u6.u5 tri-snrnp-associated protein 1 (90 kDa, Protein from Human)
- U6 snrna-associated sm-like protein lsm8 (10 kDa, Protein from Human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Fully-assembled precatalytic spliceosome (pre-b complex). (Complex from Homo sapiens)
- U4/u6 small nuclear ribonucleoprotein prp31 (55 kDa, Protein from Human)
- Splicing factor 3b subunit 2 (100 kDa, Protein from Human)
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from Human)
- Pre-mrna-processing-splicing factor 8 (271 kDa, Protein from Human)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Human)
- U6 snrna-associated sm-like protein lsm3 (11 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Human)
- Splicing factor 3b subunit 3 (135 kDa, Protein from Human)
- U4/u6 small nuclear ribonucleoprotein prp3 (77 kDa, Protein from Human)
- Splicing factor 3b subunit 5 (10 kDa, Protein from Human)
- Adml pre-mrna (Complex from Human adenovirus 2)
- U6 snrna-associated sm-like protein lsm4 (15 kDa, Protein from Human)
- Rna-binding protein 42 (50 kDa, Protein from Human)
- U4 snrna (46 kDa, RNA from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Phd finger-like domain-containing protein 5a (11 kDa, Protein from Human)
- U6 snrna-associated sm-like protein lsm5 (9 kDa, Protein from Human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Human)
- U2 snrna (60 kDa, RNA from Homo sapiens)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Human)
- U4/u6.u5 tri-snrnp-associated protein 2 (64 kDa, Protein from Human)
- U6 snrna-associated sm-like protein lsm2 (10 kDa, Protein from Human)
- Splicing factor 3a subunit 1,splicing factor 3a subunit 1,splicing factor 3a subunit 1 (71 kDa, Protein from Human)
- U6 snrna-associated sm-like protein lsm7 (11 kDa, Protein from Human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Human)
- Splicing factor 3b subunit 4 (44 kDa, Protein from Human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Human)
- Probable atp-dependent rna helicase ddx23 (Complex from Homo sapiens)
- U1 small nuclear ribonucleoprotein a (31 kDa, Protein from Human)
- U4/u6 small nuclear ribonucleoprotein prp4 (58 kDa, Protein from Human)
- Thioredoxin-like protein 4a (16 kDa, Protein from Human)
- Fully-assembled precatalytic spliceosome (pre-b complex). (3 MDa, Complex)
- Magnesium ion (24 Da, Ligand)
- U6 snrna (34 kDa, RNA from Homo sapiens)
- U1 snrna (52 kDa, RNA from Homo sapiens)
- Splicing factor 3a subunit 2 (24 kDa, Protein from Human)
- U6 snrna-associated sm-like protein lsm6 (9 kDa, Protein from Human)
- Probable atp-dependent rna helicase ddx23 (95 kDa, Protein from Homo sapiens)
- Adml pre-mrna (15 kDa, RNA from Human adenovirus 2)
- U1 small nuclear ribonucleoprotein 70 kda (51 kDa, Protein from Human)
- Splicing factor 3a subunit 3 (58 kDa, Protein from Human)
- U5 snrna (37 kDa, RNA from Homo sapiens)
- Splicing factor 3b subunit 1 (146 kDa, Protein from Human)
- U1 small nuclear ribonucleoprotein c (17 kDa, Protein from Human)
- Serine/threonine-protein kinase prp4 homolog (117 kDa, Protein from Human)