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EMBL

was set up in 1974 as Europe’s flagship laboratory for the life sciences – an intergovernmental organisation with more than 80 independent research groups covering the spectrum of molecular biology:

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perform basic research in molecular biology

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offer vital services to scientists in the member states

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scientists, students and visitors at all levels

Transfer

and development of technology

Develop

new instruments and methods

Integrating

life science research in Europe

More about EMBL

Six sites

represent EMBL in Europe.

Barcelona

Tissue biology and disease modelling

Grenoble

Structural biology

Hamburg

Structural biology

Heidelberg

Main laboratory

Hinxton

EMBL-EBI: European Bioinformatics Institute

Rome

Epigenetics and neurobiology

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sample_name:"Pre-mRNA-processing-splicing factor 8" AND software_ft:"GCTF" AND author_name:"Zhan X"

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EMD-6889

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cryo-EM structure of a human activated spliceosome (mature Bact) at 5.1 angstrom.

Release date: Sept. 19, 2018
Resolution: 5.1 Å
EM Method: Single-particle
Fitted PDBs: 5z56
Q-score: 0.131

Zhang X, Yan C, Zhan X, Li L, Lei J, Shi Y

Cell Res (2018) 28 pp. 307-322 [ DOI: doi:10.1038/cr.2018.14 Pubmed: 29360106 ]

Sample:

Peptidyl-prolyl cis-trans isomerase cwc27 homolog (53 kDa, Protein from Human)
Splicing factor 3b subunit 1 (146 kDa, Protein from Human)
116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Human)
Splicing factor 3b subunit 6 (14 kDa, Protein from Human)
Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from Human)
Pre-mrna-splicing factor syf1 (100 kDa, Protein from Human)
U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Human)
Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from Human)
Guanosine-5'-triphosphate (523 Da, Ligand)
Splicing factor 3a subunit 3 (58 kDa, Protein from Human)
U6 snrna (34 kDa, RNA from Human)
Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Human)
Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Human)
Splicing factor 3a subunit 2 (49 kDa, Protein from Human)
Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from Human)
Ring finger protein 113a (38 kDa, Protein from Human)
Small nuclear ribonucleoprotein-associated proteins b and b' (23 kDa, Protein from Human)
Magnesium ion (24 Da, Ligand)
U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Human)
Smad nuclear-interacting protein 1 (45 kDa, Protein from Human)
Protein bud31 homolog (17 kDa, Protein from Human)
Inositol hexakisphosphate (660 Da, Ligand)
Phd finger-like domain-containing protein 5a (12 kDa, Protein from Human)
Splicing factor 3b subunit 5 (10 kDa, Protein from Human)
U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Human)
U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Human)
U2 snrna (60 kDa, RNA from Human)
Pleiotropic regulator 1 (57 kDa, Protein from Human)
Cell division cycle 5-like protein (92 kDa, Protein from Human)
Splicing factor 3b subunit 2 (100 kDa, Protein from Human)
Splicing factor 3b subunit 4 (44 kDa, Protein from Human)
Zinc ion (65 Da, Ligand)
Pre-mrna-splicing factor spf27 (26 kDa, Protein from Human)
Human activated spliceosome (Complex from Homo sapiens)
U5 snrna (37 kDa, RNA from Human)
Small nuclear ribonucleoprotein e (10 kDa, Protein from Human)
Small nuclear ribonucleoprotein f (9 kDa, Protein from Human)
Splicing factor 3a subunit 1 (88 kDa, Protein from Human)
Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Human)
Pre-mrna-processing factor 19 (55 kDa, Protein from Human)
Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Human)
Skip (62 kDa, Protein from Human)
Pre-mrna-processing factor 17 (65 kDa, Protein from Human)
Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Human)
Peptidyl-prolyl cis-trans isomerase e (33 kDa, Protein from Human)
Intron-binding protein aquarius (171 kDa, Protein from Human)
Pre-mrna-splicing factor rbm22 (46 kDa, Protein from Human)
Splicing factor 3b subunit 3 (135 kDa, Protein from Human)
Crooked neck-like protein 1 (100 kDa, Protein from Human)
Putative pre-mrna-splicing factor atp-dependent rna helicase dhx16 (119 kDa, Protein from Human)
Rna-binding motif protein, x-linked 2 (37 kDa, Protein from Human)
Small nuclear ribonucleoprotein g (8 kDa, Protein from Human)
Pre-mrna (87 kDa, RNA from unidentified adenovirus)
Bud13 homolog (70 kDa, Protein from Human)

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EMD-6890

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Cryo-EM structure of the human activated spliceosome (late Bact) at 6.5 angstrom

Release date: Sept. 19, 2018
Resolution: 6.5 Å
EM Method: Single-particle
Fitted PDBs: 5z57
Q-score: 0.097

Zhang X, Yan C, Zhan X, Li L, Lei J, Shi Y

Cell Res (2018) 28 pp. 307-322 [ DOI: doi:10.1038/cr.2018.14 Pubmed: 29360106 ]

Sample:

Late bact splieosome (Complex from Homo sapiens)
Splicing factor 3b subunit 1 (146 kDa, Protein from Human)
116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Human)
Splicing factor 3b subunit 6 (14 kDa, Protein from Human)
Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from Human)
Pre-mrna-splicing factor syf1 (100 kDa, Protein from Human)
U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Human)
Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from Human)
Guanosine-5'-triphosphate (523 Da, Ligand)
Splicing factor 3a subunit 3 (58 kDa, Protein from Human)
U6 snrna (34 kDa, RNA from Human)
Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Human)
Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Human)
Splicing factor 3a subunit 2 (49 kDa, Protein from Human)
Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from Human)
Magnesium ion (24 Da, Ligand)
Small nuclear ribonucleoprotein-associated proteins b and b' (23 kDa, Protein from Human)
U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Human)
Smad nuclear-interacting protein 1 (45 kDa, Protein from Human)
Protein bud31 homolog (17 kDa, Protein from Human)
Inositol hexakisphosphate (660 Da, Ligand)
Phd finger-like domain-containing protein 5a (12 kDa, Protein from Human)
Splicing factor 3b subunit 5 (10 kDa, Protein from Human)
U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Human)
U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Human)
U2 snrna (60 kDa, RNA from Human)
Pleiotropic regulator 1 (57 kDa, Protein from Human)
Cell division cycle 5-like protein (92 kDa, Protein from Human)
Splicing factor 3b subunit 2 (100 kDa, Protein from Human)
Splicing factor 3b subunit 4 (44 kDa, Protein from Human)
Zinc ion (65 Da, Ligand)
Pre-mrna-splicing factor spf27 (26 kDa, Protein from Human)
U5 snrna (37 kDa, RNA from Human)
Small nuclear ribonucleoprotein e (10 kDa, Protein from Human)
Small nuclear ribonucleoprotein f (9 kDa, Protein from Human)
Splicing factor 3a subunit 1 (88 kDa, Protein from Human)
Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Human)
Pre-mrna-processing factor 19 (55 kDa, Protein from Human)
Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Human)
Skip (62 kDa, Protein from Human)
Pre-mrna-processing factor 17 (65 kDa, Protein from Human)
Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Human)
Peptidyl-prolyl cis-trans isomerase e (33 kDa, Protein from Human)
Intron-binding protein aquarius (171 kDa, Protein from Human)
Pre-mrna-splicing factor rbm22 (46 kDa, Protein from Human)
Splicing factor 3b subunit 3 (135 kDa, Protein from Human)
Crooked neck-like protein 1 (100 kDa, Protein from Human)
Alanine (89 Da, Ligand)
Rna-binding motif protein, x-linked 2 (37 kDa, Protein from Human)
Small nuclear ribonucleoprotein g (8 kDa, Protein from Human)
Pre-mrna-splicing factor atp-dependent rna helicase dhx16 (119 kDa, Protein from Human)
Pre-mrna (87 kDa, RNA from unidentified adenovirus)
Bud13 homolog (70 kDa, Protein from Human)

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EMD-6891

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Cryo-EM structure of a human activated spliceosome (early Bact) at 4.9 angstrom.

Release date: Sept. 19, 2018
Resolution: 4.9 Å
EM Method: Single-particle
Fitted PDBs: 5z58
Q-score: 0.131

Zhang X, Yan C, Zhan X, Li L, Lei J, Shi Y

Cell Res (2018) 28 pp. 307-322 [ DOI: doi:10.1038/cr.2018.14 Pubmed: 29360106 ]

Sample:

Inositol hexakisphosphate (660 Da, Ligand)
U2 snrna (60 kDa, RNA from Human)
Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from Human)
U5 snrna (37 kDa, RNA from Human)
U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Human)
Crooked neck-like protein 1 (100 kDa, Protein from Human)
Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Human)
Pre-mrna-splicing factor atp-dependent rna helicase dhx16 (119 kDa, Protein from Human)
U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Human)
Smad nuclear-interacting protein 1 (45 kDa, Protein from Human)
Rna-binding motif protein, x-linked 2 (37 kDa, Protein from Human)
Guanosine-5'-triphosphate (523 Da, Ligand)
116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Human)
Splicing factor 3b subunit 2 (100 kDa, Protein from Human)
Bud13 homolog (70 kDa, Protein from Human)
U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Human)
Ring finger protein 113a (38 kDa, Protein from Human)
Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Human)
U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Human)
Pre-mrna (87 kDa, RNA from unidentified adenovirus)
Splicing factor 3b subunit 6 (14 kDa, Protein from Human)
Splicing factor 3b subunit 3 (135 kDa, Protein from Human)
U6 snrna (34 kDa, RNA from Human)
Splicing factor 3a subunit 1 (88 kDa, Protein from Human)
Skip (62 kDa, Protein from Human)
Small nuclear ribonucleoprotein-associated proteins b and b' (23 kDa, Protein from Human)
Splicing factor 3a subunit 2 (49 kDa, Protein from Human)
Splicing factor 3b subunit 5 (10 kDa, Protein from Human)
Pleiotropic regulator 1 (57 kDa, Protein from Human)
Zinc ion (65 Da, Ligand)
Cell division cycle 5-like protein (92 kDa, Protein from Human)
Small nuclear ribonucleoprotein f (9 kDa, Protein from Human)
Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Human)
Early bact spliceosome (Complex from Homo sapiens)
Small nuclear ribonucleoprotein e (10 kDa, Protein from Human)
Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Human)
Small nuclear ribonucleoprotein g (8 kDa, Protein from Human)
Splicing factor 3b subunit 4 (44 kDa, Protein from Human)
Magnesium ion (24 Da, Ligand)
Splicing factor 3a subunit 3 (58 kDa, Protein from Human)
Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Human)
Phd finger-like domain-containing protein 5a (12 kDa, Protein from Human)
Splicing factor 3b subunit 1 (146 kDa, Protein from Human)
Peptidyl-prolyl cis-trans isomerase cwc27 homolog (53 kDa, Protein from Human)

[Show more]

EMD-6864

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The Cryo-EM Structure of Human Catalytic Step I Spliceosome (C complex) at 4.1 angstrom resolution

Release date: Aug. 8, 2018
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 5yzg
Q-score: 0.205

Zhan X, Yan C, Zhang X, Lei J, Shi Y

Science (2018) 359 pp. 537-545 [ DOI: doi:10.1126/science.aar6401 Pubmed: 29301961 ]

Sample:

U2 snrna (60 kDa, RNA from Homo sapiens)
Inositol hexakisphosphate (660 Da, Ligand)
Rna-binding protein 8a (19 kDa, Protein from Human)
Snw domain-containing protein 1 (61 kDa, Protein from Human)
Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from Human)
U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Human)
Crooked neck-like protein 1 (100 kDa, Protein from Human)
Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Human)
U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Human)
Adenosine-5'-diphosphate (427 Da, Ligand)
Pre-mrna-processing factor 17 (65 kDa, Protein from Human)
Coiled-coil domain-containing protein 94 (37 kDa, Protein from Human)
116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Human)
Guanosine-5'-triphosphate (523 Da, Ligand)
U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Human)
Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Human)
Small nuclear ribonucleoprotein-associated proteins b and b' (23 kDa, Protein from Human)
U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Human)
Pre-mrna-splicing factor cwc25 homolog (49 kDa, Protein from Human)
Pleiotropic regulator 1 (57 kDa, Protein from Human)
Intron-binding protein aquarius (171 kDa, Protein from Human)
Peptidylprolyl isomerase domain and wd repeat-containing protein 1 (73 kDa, Protein from Human)
Pre-mrna-splicing factor atp-dependent rna helicase prp16 (140 kDa, Protein from Human)
Zinc ion (65 Da, Ligand)
Unknow (2 kDa, Protein from Homo sapiens)
Adenosine-5'-triphosphate (507 Da, Ligand)
Peptidyl-prolyl cis-trans isomerase e (33 kDa, Protein from Human)
Cell division cycle 5-like protein (92 kDa, Protein from Human)
Pre-mrna-processing factor 19 (55 kDa, Protein from Human)
Small nuclear ribonucleoprotein f (9 kDa, Protein from Human)
Protein casc3 (76 kDa, Protein from Human)
Pre-mrna-splicing factor isy1 homolog (35 kDa, Protein from Human)
Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from Human)
Protein bud31 homolog (17 kDa, Protein from Human)
Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Human)
Pre-mrna (88 kDa, RNA from Human)
Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from Human)
Pre-mrna-splicing factor rbm22 (46 kDa, Protein from Human)
Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Human)
U6 snrna (34 kDa, RNA from Homo sapiens)
Small nuclear ribonucleoprotein e (10 kDa, Protein from Human)
Small nuclear ribonucleoprotein g (8 kDa, Protein from Human)
Magnesium ion (24 Da, Ligand)
Pre-mrna-splicing factor spf27 (26 kDa, Protein from Human)
Eukaryotic initiation factor 4a-iii (46 kDa, Protein from Human)
Peptidyl-prolyl cis-trans isomerase g (88 kDa, Protein from Human)
Pre-mrna-splicing factor syf1 (100 kDa, Protein from Human)
Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Human)
Pre-mrna-splicing factor syf2 (28 kDa, Protein from Human)
U5 snrna (37 kDa, RNA from Homo sapiens)
Protein mago nashi homolog 2 (17 kDa, Protein from Human)
Human catalytic step i spliceosome (Complex from Homo sapiens)

[Show more]

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EMDB [4]

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