Cryo-EM structure of dolphin Prestin in low Cl buffer
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8uc1
Q-score: 0.471
Lin X, Haller PR, Bavi N, Faruk N, Perozo E, Sosnick TR
eLife (2023) 12 [ DOI: doi:10.7554/eLife.89635 Pubmed: 38054956 ]
- Dolphin prestin solubilized in gdn in low cl buffer (Complex from Tursiops truncatus)
- Chloride ion (35 Da, Ligand)
- Prestin (80 kDa, Protein from Tursiops truncatus)
Thermostabilized human prestin in complex with chloride
Resolution: 3.52 Å
EM Method: Single-particle
Fitted PDBs: 7v73
Q-score: 0.488
Futamata H, Fukuda M, Umeda R, Yamashita K, Tomita A, Takahashi S, Shikakura T, Hayashi S, Kusakizako T, Nishizawa T, Homma K, Nureki O
Nat Commun (2022) 13 pp. 6208-6208 [ DOI: doi:10.1038/s41467-022-34017-x Pubmed: 36266333 ]
- Cholesterol (386 Da, Ligand)
- Chloride ion (35 Da, Ligand)
- Prestin (82 kDa, Protein from Homo sapiens)
- 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (760 Da, Ligand)
- Prestin in complex with chloride ion (Complex from Homo sapiens)
Thermostabilized human prestin in complex with sulfate
Resolution: 3.63 Å
EM Method: Single-particle
Fitted PDBs: 7v74
Q-score: 0.5
Futamata H, Fukuda M, Umeda R, Yamashita K, Tomita A, Takahashi S, Shikakura T, Hayashi S, Kusakizako T, Nishizawa T, Homma K, Nureki O
Nat Commun (2022) 13 pp. 6208-6208 [ DOI: doi:10.1038/s41467-022-34017-x Pubmed: 36266333 ]
- Cholesterol (386 Da, Ligand)
- Sulfate ion (96 Da, Ligand)
- 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (760 Da, Ligand)
- Prestin (82 kDa, Protein from Homo sapiens)
- Prestin in complex with chloride ion (Complex from Homo sapiens)
Thermostabilized human prestin in complex with salicylate
Resolution: 3.57 Å
EM Method: Single-particle
Fitted PDBs: 7v75
Q-score: 0.488
Futamata H, Fukuda M, Umeda R, Yamashita K, Tomita A, Takahashi S, Shikakura T, Hayashi S, Kusakizako T, Nishizawa T, Homma K, Nureki O
Nat Commun (2022) 13 pp. 6208-6208 [ DOI: doi:10.1038/s41467-022-34017-x Pubmed: 36266333 ]
- Cholesterol (386 Da, Ligand)
- 2-hydroxybenzoic acid (138 Da, Ligand)
- 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (760 Da, Ligand)
- Prestin (82 kDa, Protein from Homo sapiens)
- Prestin in complex with chloride ion (Complex from Homo sapiens)
Cryo-EM structure of prestin from gerbil (Meriones unguiculatus) in the presence of NaCl
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7sun
Q-score: 0.444
Butan C, Song Q, Bai JP, Tan WJT, Navaratnam D, Santos-Sacchi J
Nat Commun (2022) 13 pp. 290-290 [ DOI: doi:10.1038/s41467-021-27915-z Pubmed: 35022426 ]
Cryo-EM Structure of dolphin Prestin: Sensor Up (compact) state
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7s8x
Q-score: 0.506
Bavi N, Clark MD, Contreras GF, Shen R, Reddy BG, Milewski W, Perozo E
Nature (2021) 600 pp. 553-558 [ DOI: doi:10.1038/s41586-021-04152-4 Pubmed: 34695838 ]
Cryo-EM Structure of dolphin Prestin: Inhibited I (Chloride + Salicylate)
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7s9a
Q-score: 0.459
Bavi N, Clark MD, Contreras GF, Shen R, Reddy BG, Milewski W, Perozo E
Nature (2021) 600 pp. 553-558 [ DOI: doi:10.1038/s41586-021-04152-4 Pubmed: 34695838 ]
Cryo-EM Structure of dolphin Prestin: Sensor Down I (Expanded) state
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 7s9b
Q-score: 0.367
Bavi N, Clark MD, Contreras GF, Shen R, Reddy BG, Milewski W, Perozo E
Nature (2021) 600 pp. 553-558 [ DOI: doi:10.1038/s41586-021-04152-4 Pubmed: 34695838 ]
- Dolphin prestin: sensor down i (expanded) state (Complex from Tursiops truncatus)
- Prestin (80 kDa, Protein from Tursiops truncatus)
Cryo-EM Structure of dolphin Prestin: Sensor Down II (Expanded II) state
Resolution: 6.7 Å
EM Method: Single-particle
Fitted PDBs: 7s9c
Q-score: 0.253
Bavi N, Clark MD, Contreras GF, Shen R, Reddy BG, Milewski W, Perozo E
Nature (2021) 600 pp. 553-558 [ DOI: doi:10.1038/s41586-021-04152-4 Pubmed: 34695838 ]
- Dolphin prestin: sensor down ii (expanded ii) state (Complex from Tursiops truncatus)
- Prestin (80 kDa, Protein from Tursiops truncatus)
Cryo-EM Structure of dolphin Prestin: Intermediate state
Resolution: 4.6 Å
EM Method: Single-particle
Fitted PDBs: 7s9d
Q-score: 0.327
Bavi N, Clark MD, Contreras GF, Shen R, Reddy BG, Milewski W, Perozo E
Nature (2021) 600 pp. 553-558 [ DOI: doi:10.1038/s41586-021-04152-4 Pubmed: 34695838 ]
- Prestin (80 kDa, Protein from Tursiops truncatus)
- Dolphin prestin: intermediate state (Complex from Tursiops truncatus)