In situ structure of axonemal doublet microtubules in mouse sperm with 16-nm repeat
Resolution: 4.5 Å
EM Method: Subtomogram averaging
Fitted PDBs: 8i7o
Q-score: 0.248
Tai L, Yin G, Huang X, Sun F, Zhu Y
Cell Discov (2023) 9 pp. 116-116 [ Pubmed: 37989994 DOI: doi:10.1038/s41421-023-00606-3 ]
- Cilia- and flagella-associated protein 52 (68 kDa, Protein from Mus musculus)
- Tubulin beta-4b chain (47 kDa, Protein from Mus musculus)
- Tektin-1 (48 kDa, Protein from Mus musculus)
- Ef-hand domain-containing protein 1 (75 kDa, Protein from Mus musculus)
- Dual specificity phosphatase 21 (21 kDa, Protein from Mus musculus)
- Sperm acrosome-associated protein 9 (19 kDa, Protein from Mus musculus)
- Protein fam166a (36 kDa, Protein from Mus musculus)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tektin bundle-interacting protein 1 (23 kDa, Protein from Mus musculus)
- Cilia- and flagella-associated protein 276 (18 kDa, Protein from Mus musculus)
- Cilia- and flagella-associated protein 20 (22 kDa, Protein from Mus musculus)
- Mouse sperm (Cell from Mus musculus)
- Protein fam166c (23 kDa, Protein from Mus musculus)
- Tektin-4 (52 kDa, Protein from Mus musculus)
- Ef-hand calcium-binding domain-containing protein 6 (176 kDa, Protein from Mus musculus)
- Cilia and flagella-associated protein 77 (32 kDa, Protein from Mus musculus)
- Protein flattop (20 kDa, Protein from Mus musculus)
- Tektin-5 (62 kDa, Protein from Mus musculus)
- Ef-hand domain-containing family member c2 (87 kDa, Protein from Mus musculus)
- Detyrosinated tubulin alpha-3 chain (48 kDa, Protein from Mus musculus)
- Coiled-coil domain-containing protein 105 (57 kDa, Protein from Mus musculus)
- Tektin-3 (56 kDa, Protein from Mus musculus)
- Enkurin (29 kDa, Protein from Mus musculus)
- Parkin coregulated gene protein homolog (27 kDa, Protein from Mus musculus)
- Tektin-2 (50 kDa, Protein from Mus musculus)
- Testis-expressed protein 43 (16 kDa, Protein from Mus musculus)
In situ structure of axonemal doublet microtubules in mouse sperm with 48-nm repeat
Tai L, Yin G, Huang X, Sun F, Zhu Y
Cell Discov (2023) 9 pp. 116-116 [ Pubmed: 37989994 DOI: doi:10.1038/s41421-023-00606-3 ]
- Tektin-2 (50 kDa, Protein from Mus musculus)
- Cilia- and flagella-associated protein 45 (65 kDa, Protein from Mus musculus)
- Tektin-4 (52 kDa, Protein from Mus musculus)
- Parkin coregulated gene protein homolog (27 kDa, Protein from Mus musculus)
- Protein flattop (20 kDa, Protein from Mus musculus)
- Tektin bundle-interacting protein 1 (23 kDa, Protein from Mus musculus)
- Ef-hand domain-containing family member c2 (87 kDa, Protein from Mus musculus)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Nucleoside diphosphate kinase 7 (44 kDa, Protein from Mus musculus)
- Cilia- and flagella-associated protein 276 (18 kDa, Protein from Mus musculus)
- Cilia- and flagella-associated protein 210 (65 kDa, Protein from Mus musculus)
- Piercer of microtubule wall 1 protein (18 kDa, Protein from Mus musculus)
- Tektin-3 (56 kDa, Protein from Mus musculus)
- Ef-hand calcium-binding domain-containing protein 6 (176 kDa, Protein from Mus musculus)
- Protein fam166c (23 kDa, Protein from Mus musculus)
- Testis-expressed protein 43 (16 kDa, Protein from Mus musculus)
- Tubulin beta-4b chain (47 kDa, Protein from Mus musculus)
- Cilia- and flagella-associated protein 53 (62 kDa, Protein from Mus musculus)
- Meiosis-specific nuclear structural protein 1 (60 kDa, Protein from Mus musculus)
- Coiled-coil domain-containing protein 105 (57 kDa, Protein from Mus musculus)
- Ef-hand domain-containing protein 1 (75 kDa, Protein from Mus musculus)
- Detyrosinated tubulin alpha-3 chain (48 kDa, Protein from Mus musculus)
- Cilia and flagella-associated protein 77 (32 kDa, Protein from Mus musculus)
- Cilia- and flagella-associated protein 20 (22 kDa, Protein from Mus musculus)
- Sperm-associated antigen 8 (50 kDa, Protein from Mus musculus)
- Mouse sperm (Cell from Mus musculus)
- Enkurin (29 kDa, Protein from Mus musculus)
- Tektin-1 (48 kDa, Protein from Mus musculus)
- Piercer of microtubule wall 2 protein (13 kDa, Protein from Mus musculus)
- Protein fam166a (36 kDa, Protein from Mus musculus)
- Ef-hand domain-containing family member b (95 kDa, Protein from Mus musculus)
- Sperm acrosome-associated protein 9 (19 kDa, Protein from Mus musculus)
- Cilia- and flagella-associated protein 141 (12 kDa, Protein from Mus musculus)
- Dual specificity phosphatase 21 (21 kDa, Protein from Mus musculus)
- Cilia- and flagella-associated protein 52 (68 kDa, Protein from Mus musculus)
- Cilia- and flagella-associated protein 161 (34 kDa, Protein from Mus musculus)
- Cilia- and flagella-associated protein 107 (23 kDa, Protein from Mus musculus)
- Rib43a-like with coiled-coils protein 2 (45 kDa, Protein from Mus musculus)
- Cilia- and flagella-associated protein 95 (26 kDa, Protein from Mus musculus)
- Tektin-5 (62 kDa, Protein from Mus musculus)
Cryo-EM structure of the 48-nm repeat doublet microtubule from mouse sperm
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8iyj
Q-score: 0.35
Zhou L, Liu H, Liu S, Yang X, Dong Y, Pan Y, Xiao Z, Zheng B, Sun Y, Huang P, Zhang X, Hu J, Sun R, Feng S, Zhu Y, Liu M, Gui M, Wu J
Cell (2023) 186 pp. 2897-2910.e19 [ Pubmed: 37295417 DOI: doi:10.1016/j.cell.2023.05.009 ]
- Ef-hand domain-containing family member b (95 kDa, Protein from house mouse)
- Tektin-1 (48 kDa, Protein from house mouse)
- Tektin-4 (52 kDa, Protein from house mouse)
- Ef-hand domain-containing family member c2 (87 kDa, Protein from house mouse)
- Rib43a-like with coiled-coils protein 1 (45 kDa, Protein from house mouse)
- Cilia- and flagella-associated protein 90 (20 kDa, Protein from house mouse)
- Dual specificity phosphatase 21 (21 kDa, Protein from house mouse)
- Testis-expressed sequence 37 protein (21 kDa, Protein from house mouse)
- Tubulin alpha-3 chain (50 kDa, Protein from house mouse)
- Cilia- and flagella-associated protein 20 (22 kDa, Protein from house mouse)
- Enkurin (29 kDa, Protein from house mouse)
- Protein flattop (20 kDa, Protein from house mouse)
- Piercer of microtubule wall 1 protein (18 kDa, Protein from house mouse)
- Protein fam166c (23 kDa, Protein from house mouse)
- Cilia- and flagella-associated protein 95 (26 kDa, Protein from house mouse)
- Parkin coregulated gene protein homolog (27 kDa, Protein from house mouse)
- Ef-hand domain-containing protein 1 (75 kDa, Protein from house mouse)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Cilia- and flagella-associated protein 107 (23 kDa, Protein from house mouse)
- Testis, prostate and placenta-expressed protein (24 kDa, Protein from house mouse)
- Magnesium ion (24 Da, Ligand)
- Cilia- and flagella-associated protein 45 (65 kDa, Protein from house mouse)
- Sperm-associated antigen 8 (50 kDa, Protein from house mouse)
- Outer dense fiber protein 3 (27 kDa, Protein from house mouse)
- Cilia- and flagella-associated protein 141 (12 kDa, Protein from house mouse)
- Mouse sperm dmt (Complex from Mus musculus)
- Piercer of microtubule wall 2 protein (13 kDa, Protein from house mouse)
- Tektin-2 (50 kDa, Protein from house mouse)
- Tubulin beta-4b chain (49 kDa, Protein from house mouse)
- Testis-expressed protein 43 (16 kDa, Protein from house mouse)
- Cilia and flagella-associated protein 77 (32 kDa, Protein from house mouse)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Ef-hand calcium-binding domain-containing protein 6 (176 kDa, Protein from house mouse)
- Uncharacterized protein c10orf82 homolog (25 kDa, Protein from house mouse)
- Cilia- and flagella-associated protein 210 (65 kDa, Protein from house mouse)
- Tektin-3 (56 kDa, Protein from house mouse)
- Meiosis-specific nuclear structural protein 1 (60 kDa, Protein from house mouse)
- Testis expressed 49 (15 kDa, Protein from house mouse)
- Testis-specific serine/threonine-protein kinase 6 (30 kDa, Protein from house mouse)
- Cilia- and flagella-associated protein 161 (34 kDa, Protein from house mouse)
- Cilia- and flagella-associated protein 276 (18 kDa, Protein from house mouse)
- Protein fam166a (36 kDa, Protein from house mouse)
- Cilia- and flagella-associated protein 68 (19 kDa, Protein from house mouse)
- Coiled-coil domain-containing protein 105 (57 kDa, Protein from house mouse)
- Tektin-5 (62 kDa, Protein from house mouse)
- Protein phosphatase 1 regulatory subunit 32 (47 kDa, Protein from house mouse)
- Spermatid-specific manchette-related protein 1 (29 kDa, Protein from house mouse)
- Nucleoside diphosphate kinase 7 (44 kDa, Protein from house mouse)
- Cilia- and flagella-associated protein 53 (62 kDa, Protein from house mouse)
- Cilia- and flagella-associated protein 52 (68 kDa, Protein from house mouse)
- Tektin bundle-interacting protein 1 (23 kDa, Protein from house mouse)
- Rib43a-like with coiled-coils protein 2 (45 kDa, Protein from house mouse)
- Family with sequence similarity 183, member b (16 kDa, Protein from house mouse)