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was set up in 1974 as Europe’s flagship laboratory for the life sciences – an intergovernmental organisation with more than 80 independent research groups covering the spectrum of molecular biology:

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perform basic research in molecular biology

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offer vital services to scientists in the member states

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Six sites

represent EMBL in Europe.

Barcelona

Tissue biology and disease modelling

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Structural biology

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Structural biology

Heidelberg

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Hinxton

EMBL-EBI: European Bioinformatics Institute

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Epigenetics and neurobiology

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sample_name:"Protein mago nashi homolog 2"

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EMD-9645

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Cryo-EM structure of a human post-catalytic spliceosome (P complex) at 3.0 angstrom

Release date: March 13, 2019
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6icz
Q-score: 0.284

Zhang X, Zhan X, Yan C, Zhang W, Liu D, Lei J, Shi Y

Cell Res (2019) 29 pp. 274-285 [ DOI: doi:10.1038/s41422-019-0143-x Pubmed: 30728453 ]

Sample:

U2snrna (60 kDa, RNA from Human)
116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Human)
Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from Human)
Human post_catalytic spliceosome (Complex from Homo sapiens)
Pre-mrna-splicing factor syf1 (100 kDa, Protein from Human)
Rna helicase aquarius (171 kDa, Protein from Human)
Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from Human)
U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Human)
Guanosine-5'-triphosphate (523 Da, Ligand)
Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Human)
Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Human)
Atp-dependent rna helicase dhx8 (139 kDa, Protein from Human)
Adenosine-5'-triphosphate (507 Da, Ligand)
U6snrna (34 kDa, RNA from Human)
Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from Human)
Magnesium ion (24 Da, Ligand)
Rna-binding protein 8a (19 kDa, Protein from Human)
U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Human)
Protein bud31 homolog (17 kDa, Protein from Human)
Pre-mrna-splicing factor syf2 (28 kDa, Protein from Human)
Pre-mrna-splicing factor slu7 (68 kDa, Protein from Human)
Small nuclear ribonucleoprotein-associated protein (23 kDa, Protein from Human)
Inositol hexakisphosphate (660 Da, Ligand)
U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Human)
U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Human)
Prkr-interacting protein 1 (21 kDa, Protein from Human)
Pleiotropic regulator 1 (57 kDa, Protein from Human)
Cell division cycle 5-like protein (92 kDa, Protein from Human)
Zinc ion (65 Da, Ligand)
Protein mago nashi homolog 2 (17 kDa, Protein from Human)
Snw domain-containing protein 1 (61 kDa, Protein from Human)
Pre-mrna-splicing factor spf27 (26 kDa, Protein from Human)
Protein casc3 (76 kDa, Protein from Human)
Pre-mrna-processing factor 19 (55 kDa, Protein from Human)
Peptidyl-prolyl cis-trans isomerase e (33 kDa, Protein from Human)
Small nuclear ribonucleoprotein f (9 kDa, Protein from Human)
Pre-mrna-processing factor 17 (65 kDa, Protein from Human)
Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Human)
Pre-mrna (87 kDa, RNA from Human)
Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Human)
U5snrna (37 kDa, RNA from Human)
Eukaryotic initiation factor 4a-iii (46 kDa, Protein from Human)
Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Human)
Small nuclear ribonucleoprotein e (10 kDa, Protein from Human)
Pre-mrna-splicing factor rbm22 (46 kDa, Protein from Human)
Crooked neck-like protein 1 (100 kDa, Protein from Human)
Small nuclear ribonucleoprotein g (8 kDa, Protein from Human)

[Show more]

EMD-4525

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Human post-catalytic spliceosome (P complex) stalled with DHX8 K594A mutant, overall map

Release date: Feb. 20, 2019
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6qdv
Q-score: 0.278

Fica SM, Oubridge C, Wilkinson ME, Newman AJ, Nagai K

Science (2019) 363 pp. 710-714 [ Pubmed: 30705154 DOI: doi:10.1126/science.aaw5569 ]

Sample:

Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Homo sapiens)
Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from Homo sapiens)
Atp-dependent rna helicase dhx8 (Complex from Homo sapiens)
Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from Homo sapiens)
Pre-mrna-splicing factor syf1 (100 kDa, Protein from Homo sapiens)
Small nuclear ribonucleoprotein e (9 kDa, Protein from Homo sapiens)
Protein fam32a (7 kDa, Protein from Homo sapiens)
Ligated exons: minx mrna (4 kDa, RNA from Human adenovirus 2)
Snw domain-containing protein 1 (33 kDa, Protein from Homo sapiens)
Pre-mrna-splicing factor spf27 (26 kDa, Protein from Homo sapiens)
Guanosine-5'-triphosphate (523 Da, Ligand)
Intron lariat: minx rna (27 kDa, RNA from Human adenovirus 2)
Pre-mrna-processing factor 17 (59 kDa, Protein from Homo sapiens)
Pleiotropic regulator 1 (35 kDa, Protein from Homo sapiens)
Inositol hexakisphosphate (660 Da, Ligand)
Pre-mrna-processing factor 19 (55 kDa, Protein from Homo sapiens)
Replication stress response regulator sde2 (4 kDa, Protein from Homo sapiens)
Atp-dependent rna helicase dhx8 (139 kDa, Protein from Homo sapiens)
Serine/arginine repetitive matrix protein 2 (2 kDa, Protein from Homo sapiens)
Potassium ion (39 Da, Ligand)
Pre-mrna-splicing factor slu7 (68 kDa, Protein from Homo sapiens)
116 kda u5 small nuclear ribonucleoprotein component (101 kDa, Protein from Homo sapiens)
Crooked neck-like protein 1 (100 kDa, Protein from Homo sapiens)
Small nuclear ribonucleoprotein-associated proteins b and b' (9 kDa, Protein from Homo sapiens)
U6 snrna (34 kDa, RNA from Homo sapiens)
Pre-mrna-splicing factor syf2 (17 kDa, Protein from Homo sapiens)
Zinc ion (65 Da, Ligand)
Pre-mrna-splicing factor rbm22 (33 kDa, Protein from Homo sapiens)
Adenosine-5'-triphosphate (507 Da, Ligand)
Prkr-interacting protein 1 (14 kDa, Protein from Homo sapiens)
Cell division cycle 5-like protein (92 kDa, Protein from Homo sapiens)
Eukaryotic initiation factor 4a-iii (44 kDa, Protein from Homo sapiens)
Cactin (15 kDa, Protein from Homo sapiens)
Small nuclear ribonucleoprotein g (8 kDa, Protein from Homo sapiens)
Small nuclear ribonucleoprotein sm d3 (9 kDa, Protein from Homo sapiens)
Magnesium ion (24 Da, Ligand)
U2 snrna (60 kDa, RNA from Homo sapiens)
Human post-catalytic p complex spliceosome (Complex from Homo sapiens)
Intron-binding protein aquarius (171 kDa, Protein from Homo sapiens)
U5 small nuclear ribonucleoprotein 40 kda protein (34 kDa, Protein from Homo sapiens)
U2 small nuclear ribonucleoprotein b'' (10 kDa, Protein from Homo sapiens)
Nf-kappa-b-activating protein (3 kDa, Protein from Homo sapiens)
Small nuclear ribonucleoprotein f (8 kDa, Protein from Homo sapiens)
Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Homo sapiens)
U5 small nuclear ribonucleoprotein 200 kda helicase (197 kDa, Protein from Homo sapiens)
Small nuclear ribonucleoprotein sm d1 (9 kDa, Protein from Homo sapiens)
Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Homo sapiens)
U5 snrna (36 kDa, RNA from Homo sapiens)
Human post-catalytic p complex spliceosome (3 MDa, Complex)
Rna-binding protein 8a (10 kDa, Protein from Homo sapiens)
Protein bud31 homolog (17 kDa, Protein from Homo sapiens)
Minx introns and exons (Complex from Human adenovirus 2)
U2 small nuclear ribonucleoprotein a' (18 kDa, Protein from Homo sapiens)
Protein mago nashi homolog 2 (16 kDa, Protein from Homo sapiens)

[Show more]

EMD-6864

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The Cryo-EM Structure of Human Catalytic Step I Spliceosome (C complex) at 4.1 angstrom resolution

Release date: Aug. 8, 2018
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 5yzg
Q-score: 0.205

Zhan X, Yan C, Zhang X, Lei J, Shi Y

Science (2018) 359 pp. 537-545 [ DOI: doi:10.1126/science.aar6401 Pubmed: 29301961 ]

Sample:

U2 snrna (60 kDa, RNA from Homo sapiens)
Inositol hexakisphosphate (660 Da, Ligand)
Rna-binding protein 8a (19 kDa, Protein from Human)
Snw domain-containing protein 1 (61 kDa, Protein from Human)
Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from Human)
U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Human)
Crooked neck-like protein 1 (100 kDa, Protein from Human)
Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Human)
U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Human)
Adenosine-5'-diphosphate (427 Da, Ligand)
Pre-mrna-processing factor 17 (65 kDa, Protein from Human)
Coiled-coil domain-containing protein 94 (37 kDa, Protein from Human)
116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Human)
Guanosine-5'-triphosphate (523 Da, Ligand)
U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Human)
Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Human)
Small nuclear ribonucleoprotein-associated proteins b and b' (23 kDa, Protein from Human)
U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Human)
Pre-mrna-splicing factor cwc25 homolog (49 kDa, Protein from Human)
Pleiotropic regulator 1 (57 kDa, Protein from Human)
Intron-binding protein aquarius (171 kDa, Protein from Human)
Peptidylprolyl isomerase domain and wd repeat-containing protein 1 (73 kDa, Protein from Human)
Pre-mrna-splicing factor atp-dependent rna helicase prp16 (140 kDa, Protein from Human)
Zinc ion (65 Da, Ligand)
Unknow (2 kDa, Protein from Homo sapiens)
Adenosine-5'-triphosphate (507 Da, Ligand)
Peptidyl-prolyl cis-trans isomerase e (33 kDa, Protein from Human)
Cell division cycle 5-like protein (92 kDa, Protein from Human)
Pre-mrna-processing factor 19 (55 kDa, Protein from Human)
Small nuclear ribonucleoprotein f (9 kDa, Protein from Human)
Protein casc3 (76 kDa, Protein from Human)
Pre-mrna-splicing factor isy1 homolog (35 kDa, Protein from Human)
Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from Human)
Protein bud31 homolog (17 kDa, Protein from Human)
Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Human)
Pre-mrna (88 kDa, RNA from Human)
Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from Human)
Pre-mrna-splicing factor rbm22 (46 kDa, Protein from Human)
Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Human)
U6 snrna (34 kDa, RNA from Homo sapiens)
Small nuclear ribonucleoprotein e (10 kDa, Protein from Human)
Small nuclear ribonucleoprotein g (8 kDa, Protein from Human)
Magnesium ion (24 Da, Ligand)
Pre-mrna-splicing factor spf27 (26 kDa, Protein from Human)
Eukaryotic initiation factor 4a-iii (46 kDa, Protein from Human)
Peptidyl-prolyl cis-trans isomerase g (88 kDa, Protein from Human)
Pre-mrna-splicing factor syf1 (100 kDa, Protein from Human)
Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Human)
Pre-mrna-splicing factor syf2 (28 kDa, Protein from Human)
U5 snrna (37 kDa, RNA from Homo sapiens)
Protein mago nashi homolog 2 (17 kDa, Protein from Human)
Human catalytic step i spliceosome (Complex from Homo sapiens)

[Show more]

EMD-6721

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Cryo-EM structure of the human spliceosome just prior to exon ligation at 3.6 angstrom

Release date: July 5, 2017
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 5xjc
Q-score: 0.22

Zhang X, Yan C, Hang J, Finci LI, Lei J, Shi Y

Cell (2017) 169 pp. 918-929.e14 [ DOI: doi:10.1016/j.cell.2017.04.033 Pubmed: 28502770 ]

Sample:

Prkr-interacting protein 1 (21 kDa, Protein from Human)
Atp-dependent rna helicase dhx8 (139 kDa, Protein from Human)
Inositol hexakisphosphate (660 Da, Ligand)
Rna-binding protein 8a (19 kDa, Protein from Human)
Homo sapiens small nuclear rna (u2) gene (60 kDa, RNA from Homo sapiens)
Snw domain-containing protein 1 (61 kDa, Protein from Human)
Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from Human)
U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Human)
Pre-mrna (87 kDa, RNA from Human adenovirus 2)
Crooked neck-like protein 1 (100 kDa, Protein from Human)
Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Human)
U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Human)
Adenosine-5'-diphosphate (427 Da, Ligand)
Pre-mrna-processing factor 17 (65 kDa, Protein from Human)
Guanosine-5'-triphosphate (523 Da, Ligand)
116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Human)
U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Human)
Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Human)
U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Human)
Pleiotropic regulator 1 (57 kDa, Protein from Human)
Small nuclear ribonucleoprotein-associated proteins b and b' (23 kDa, Protein from Human)
Intron-binding protein aquarius (171 kDa, Protein from Human)
Zinc ion (65 Da, Ligand)
Adenosine-5'-triphosphate (507 Da, Ligand)
Cell division cycle 5-like protein (92 kDa, Protein from Human)
Pre-mrna-processing factor 19 (55 kDa, Protein from Human)
Small nuclear ribonucleoprotein f (9 kDa, Protein from Human)
Protein casc3 (76 kDa, Protein from Human)
Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from Human)
Pre-mrna-splicing factor slu7 (68 kDa, Protein from Human)
Protein bud31 homolog (17 kDa, Protein from Human)
Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Human)
Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from Human)
Pre-mrna-splicing factor rbm22 (46 kDa, Protein from Human)
Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Human)
U6 snrna (34 kDa, RNA from Homo sapiens)
Small nuclear ribonucleoprotein e (10 kDa, Protein from Human)
Small nuclear ribonucleoprotein g (8 kDa, Protein from Human)
Magnesium ion (24 Da, Ligand)
Pre-mrna-splicing factor spf27 (26 kDa, Protein from Human)
Eukaryotic initiation factor 4a-iii (46 kDa, Protein from Human)
Pre-mrna-splicing factor syf1 (100 kDa, Protein from Human)
Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Human)
Pre-mrna-splicing factor syf2 (28 kDa, Protein from Human)
Human c* spliceosome (Complex from Homo sapiens)
U5 snrna (37 kDa, RNA from Homo sapiens)
Protein mago nashi homolog 2 (17 kDa, Protein from Human)

[Show more]

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EMDB [4]

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Select the script type:

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