Electron Microscopy Data Bank

EMD-19582

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CRYO-EM STRUCTURE OF LEISHMANIA MAJOR 80S RIBOSOME WITH A/P/E-site tRNA AND mRNA : LM32Cs3H1 sKO STRAIN

Release date: May 15, 2024
Resolution: 2.97 Å
EM Method: Single-particle
Fitted PDBs: 8rxx
Q-score: 0.601

Rajan KS, Aryal S, Hiregange DG, Bashan A, Madmoni H, Olami M, Doniger T, Cohen-Chalamish S, Pescher P, Taoka M, Nobe Y, Fedorenko A, Bose T, Zimermann E, Prina E, Aharon-Hefetz N, Pilpel Y, Isobe T, Unger R, Spath GF, Yonath A, Michaeli S

Cell Rep (2024) 43 pp. 114203-114203 [ Pubmed: 38722744 DOI: doi:10.1016/j.celrep.2024.114203 ]

Sample:

60s ribosomal protein l18a (20 kDa, Protein from Leishmania major strain Friedlin)
Ubiquitin-60s ribosomal protein l40 (14 kDa, Protein from Leishmania major strain Friedlin)
5.8s_rrna_chain_7 (54 kDa, RNA from Leishmania major strain Friedlin)
Putative 60s ribosomal protein l28 (16 kDa, Protein from Leishmania major strain Friedlin)
Putative 40s ribosomal protein s3 (24 kDa, Protein from Leishmania major strain Friedlin)
Putative ribosomal protein l1a (41 kDa, Protein from Leishmania major strain Friedlin)
Putative rna binding protein (25 kDa, Protein from Leishmania major strain Friedlin)
Putative 60s ribosomal protein l36 (11 kDa, Protein from Leishmania major strain Friedlin)
Putative 60s ribosomal protein l34 (19 kDa, Protein from Leishmania major strain Friedlin)
40s ribosomal protein s8 (24 kDa, Protein from Leishmania major strain Friedlin)
Water (18 Da, Ligand)
A-site_trna_chain_s2 (24 kDa, RNA from Leishmania major strain Friedlin)
Putative 40s ribosomal protein s13 (17 kDa, Protein from Leishmania major strain Friedlin)
60s ribosomal protein l7a (29 kDa, Protein from Leishmania major strain Friedlin)
60s ribosomal protein l37a (10 kDa, Protein from Leishmania major strain Friedlin)
Putative 40s ribosomal protein s10 (18 kDa, Protein from Leishmania major strain Friedlin)
Putative ribosomal protein s20 (13 kDa, Protein from Leishmania major strain Friedlin)
Ribosomal protein l37 (9 kDa, Protein from Leishmania major strain Friedlin)
40s ribosomal protein s14 (15 kDa, Protein from Leishmania major strain Friedlin)
Putative 60s ribosomal protein l35 (15 kDa, Protein from Leishmania major strain Friedlin)
5s_rrna_chain_8 (39 kDa, RNA from Leishmania major strain Friedlin)
40s ribosomal protein s6 (28 kDa, Protein from Leishmania major strain Friedlin)
P-site_trna_chain_s3 (24 kDa, RNA from Leishmania major strain Friedlin)
Sodium ion (22 Da, Ligand)
60s ribosomal protein l27 (15 kDa, Protein from Leishmania major strain Friedlin)
Ubiquitin-60s ribosomal protein l40 (17 kDa, Protein from Leishmania major strain Friedlin)
Potassium ion (39 Da, Ligand)
Putative 60s ribosomal protein l23a (16 kDa, Protein from Leishmania major strain Friedlin)
Putative ribosomal protein l3 (47 kDa, Protein from Leishmania major strain Friedlin)
Ssu_rrna_chain_s1 (710 kDa, RNA from Leishmania major strain Friedlin)
Sr1_chain_3 (69 kDa, RNA from Leishmania major strain Friedlin)
Putative 40s ribosomal protein s23 (15 kDa, Protein from Leishmania major strain Friedlin)
Putative ribosomal protein l35a (16 kDa, Protein from Leishmania major strain Friedlin)
40s ribosomal protein s12 (15 kDa, Protein from Leishmania major strain Friedlin)
Putative 60s ribosomal protein l26 (16 kDa, Protein from Leishmania major strain Friedlin)
60s ribosomal protein l30 (11 kDa, Protein from Leishmania major strain Friedlin)
Putative 40s ribosomal protein s27-1 (9 kDa, Protein from Leishmania major strain Friedlin)
Putative 60s ribosomal protein l17 (19 kDa, Protein from Leishmania major strain Friedlin)
Putative 60s ribosomal protein l19 (28 kDa, Protein from Leishmania major strain Friedlin)
Putative 60s ribosomal protein l22 (15 kDa, Protein from Leishmania major strain Friedlin)
Putative 60s ribosomal subunit protein l31 (21 kDa, Protein from Leishmania major strain Friedlin)
60s ribosomal protein l18 (22 kDa, Protein from Leishmania major strain Friedlin)
E-site_trna_chain_s4 (24 kDa, RNA from Leishmania major strain Friedlin)
40s ribosomal protein s25 (13 kDa, Protein from Leishmania major strain Friedlin)
Putative 60s ribosomal protein l7 (28 kDa, Protein from Leishmania major strain Friedlin)
Putative 40s ribosomal protein s15a (14 kDa, Protein from Leishmania major strain Friedlin)
Putative 60s ribosomal protein l10 (24 kDa, Protein from Leishmania major strain Friedlin)
Lsub_rrna_chain_2 (492 kDa, RNA from Leishmania major strain Friedlin)
Putative 40s ribosomal protein l14 (19 kDa, Protein from Leishmania major strain Friedlin)
Putative 60s ribosomal protein l23 (14 kDa, Protein from Leishmania major strain Friedlin)
Putative 40s ribosomal protein s17 (16 kDa, Protein from Leishmania major strain Friedlin)
Putative 40s ribosomal protein s16 (16 kDa, Protein from Leishmania major strain Friedlin)
60s ribosomal protein l11 (21 kDa, Protein from Leishmania major strain Friedlin)
Putative 60s ribosomal protein l44 (12 kDa, Protein from Leishmania major strain Friedlin)
Putative 60s ribosomal protein l5 (34 kDa, Protein from Leishmania major strain Friedlin)
Putative 60s ribosomal protein l39 (6 kDa, Protein from Leishmania major strain Friedlin)
60s ribosomal protein l41 (4 kDa, Protein from Leishmania major strain Friedlin)
Mrna_chain_s5 (4 kDa, RNA from Leishmania major strain Friedlin)
Sr2_chain_4 (59 kDa, RNA from Leishmania major strain Friedlin)
40s ribosomal protein s24 (15 kDa, Protein from Leishmania major strain Friedlin)
40s ribosomal protein s5 (21 kDa, Protein from Leishmania major strain Friedlin)
Putative 40s ribosomal protein s33 (9 kDa, Protein from Leishmania major strain Friedlin)
Putative ribosomal protein l24 (14 kDa, Protein from Leishmania major strain Friedlin)
Magnesium ion (24 Da, Ligand)
40s ribosomal protein s3a (30 kDa, Protein from Leishmania major strain Friedlin)
Sr6_chain_6 (23 kDa, RNA from Leishmania major strain Friedlin)
Putative 60s ribosomal protein l21 (18 kDa, Protein from Leishmania major strain Friedlin)
Putative 40s ribosomal protein s18 (17 kDa, Protein from Leishmania major strain Friedlin)
Putative 60s ribosomal protein l9 (21 kDa, Protein from Leishmania major strain Friedlin)
Putative ribosomal protein l38 (9 kDa, Protein from Leishmania major strain Friedlin)
Putative 60s ribosomal protein l6 (21 kDa, Protein from Leishmania major strain Friedlin)
Sr4_chain_5 (43 kDa, RNA from Leishmania major strain Friedlin)
40s ribosomal protein s7 (23 kDa, Protein from Leishmania major strain Friedlin)
Putative 60s ribosomal protein l13a (25 kDa, Protein from Leishmania major strain Friedlin)
Putative 40s ribosomal protein s21 (17 kDa, Protein from Leishmania major strain Friedlin)
40s ribosomal protein sa (27 kDa, Protein from Leishmania major strain Friedlin)
Putative 60s ribosomal protein l13 (24 kDa, Protein from Leishmania major strain Friedlin)
40s ribosomal protein s4 (30 kDa, Protein from Leishmania major strain Friedlin)
40s ribosomal protein s2 (28 kDa, Protein from Leishmania major strain Friedlin)
Ribosomal protein s17 family protein (20 kDa, Protein from Leishmania major strain Friedlin)
60s ribosomal protein l29 (8 kDa, Protein from Leishmania major strain Friedlin)
Lsua_rrna_chain_1 (577 kDa, RNA from Leishmania major strain Friedlin)
Putative 40s ribosomal protein s9 (22 kDa, Protein from Leishmania major strain Friedlin)
Guanine nucleotide-binding protein subunit beta-like protein (34 kDa, Protein from Leishmania major strain Friedlin)
80s ribosome with a/p/e-site trna and mrna (Complex from Leishmania major strain Friedlin)
Zinc ion (65 Da, Ligand)
Putative 60s ribosomal protein l27a/l29 (16 kDa, Protein from Leishmania major strain Friedlin)
Putative ribosomal protein s29 (6 kDa, Protein from Leishmania major strain Friedlin)
Putative 40s ribosomal protein s15 (17 kDa, Protein from Leishmania major strain Friedlin)
60s ribosomal protein l32 (15 kDa, Protein from Leishmania major strain Friedlin)
40s ribosomal protein s26 (12 kDa, Protein from Leishmania major strain Friedlin)
Ribosomal protein l15 (24 kDa, Protein from Leishmania major strain Friedlin)
40s ribosomal protein s19-like protein (18 kDa, Protein from Leishmania major strain Friedlin)
40s ribosomal protein s30 (7 kDa, Protein from Leishmania major strain Friedlin)
Putative 60s ribosomal protein l2 (28 kDa, Protein from Leishmania major strain Friedlin)

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