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EMBL

was set up in 1974 as Europe’s flagship laboratory for the life sciences – an intergovernmental organisation with more than 80 independent research groups covering the spectrum of molecular biology:

Research:

perform basic research in molecular biology

Services:

offer vital services to scientists in the member states

Training

scientists, students and visitors at all levels

Transfer

and development of technology

Develop

new instruments and methods

Integrating

life science research in Europe

More about EMBL

Six sites

represent EMBL in Europe.

Barcelona

Tissue biology and disease modelling

Grenoble

Structural biology

Hamburg

Structural biology

Heidelberg

Main laboratory

Hinxton

EMBL-EBI: European Bioinformatics Institute

Rome

Epigenetics and neurobiology

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sample_name:"RNA (5'-R(P*UP*GP*UP*AP*AP*UP*CP*CP*CP*CP*UP*UP*GP*GP*CP*GP*GP*UP*U)-3')"

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EMD-33450

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RNA polymerase II elongation complex transcribing a nucleosome (EC58hex)

Release date: Oct. 12, 2022
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7xti
Q-score: 0.223

Ehara H, Kujirai T, Shirouzu M, Kurumizaka H, Sekine SI

Science (2022) 377 pp. eabp9466-eabp9466 [ DOI: doi:10.1126/science.abp9466 Pubmed: 35981082 ]

Sample:

Rna polymerase subunit abc23, common to rna polymerases i, ii, and iii (17 kDa, Protein from Komagataella phaffii)
Dna,rna (Complex)
Histone h3.3 (15 kDa, Protein from Homo sapiens)
Rna polymerase subunit abc10-alpha (7 kDa, Protein from Komagataella phaffii)
Elf1,spt4/5,spn1 and paf1c (Complex from Komagataella phaffii)
Rnapii-associated chromatin remodeling paf1 complex subunit (62 kDa, Protein from Komagataella phaffii)
Protein that interacts with spt6p and copurifies with spt5p and rna polymerase ii (46 kDa, Protein from Komagataella phaffii)
Rna polymerase ii subunit b32 (20 kDa, Protein from Komagataella phaffii)
Rna polymerase ii (Complex from Komagataella phaffii)
Dna (198-mer) (60 kDa, DNA from synthetic construct)
Leo1 (52 kDa, Protein from Komagataella phaffii)
Fact complex subunit (115 kDa, Protein from Komagataella phaffii)
Fact complex subunit pob3 (60 kDa, Protein from Komagataella phaffii)
Rna polymerase ii third largest subunit b44, part of central core (34 kDa, Protein from Komagataella phaffii)
Rna polymerase subunit abc10-beta, common to rna polymerases i, ii, and iii (8 kDa, Protein from Komagataella phaffii)
Rnap ii-associated protein (46 kDa, Protein from Komagataella phaffii)
Rna (5'-r(p*up*gp*up*ap*ap*up*cp*cp*cp*cp*up*up*gp*gp*cp*gp*gp*up*u)-3') (6 kDa, RNA from synthetic construct)
Magnesium ion (24 Da, Ligand)
Spt6 (Complex from Komagataella phaffii)
Dna (198-mer) (61 kDa, DNA from synthetic construct)
Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (24 kDa, Protein from Komagataella phaffii)
Transcription elongation factor spt6 (173 kDa, Protein from Komagataella phaffii)
Component of the paf1p complex (124 kDa, Protein from Komagataella phaffii)
Constituent of paf1 complex with rna polymerase ii, paf1p, hpr1p, ctr9, leo1, rtf1 and ccr4p (44 kDa, Protein from Komagataella phaffii)
Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (16 kDa, Protein from Komagataella phaffii)
Transcription elongation factor spt5 (101 kDa, Protein from Komagataella phaffii)
Transcription elongation factor spt4 (12 kDa, Protein from Komagataella phaffii)
Rna polymerase ii elongation complex transcribing a nucleosome (ec58hex) (Complex)
Histone h2a type 1-b/e (14 kDa, Protein from Homo sapiens)
Rna polymerase ii subunit (18 kDa, Protein from Komagataella phaffii)
Zinc ion (65 Da, Ligand)
Dna-directed rna polymerase subunit (194 kDa, Protein from Komagataella phaffii)
Dna-directed rna polymerase subunit (13 kDa, Protein from Komagataella phaffii)
Dna-directed rna polymerase subunit beta (139 kDa, Protein from Komagataella phaffii)
Histone h4 (11 kDa, Protein from Homo sapiens)
Histone, fact complex subunit (Complex from Homo sapiens)
Histone h2b type 1-j (14 kDa, Protein from Homo sapiens)
Transcription elongation factor 1 homolog (12 kDa, Protein from Komagataella phaffii)
Rna polymerase ii subunit b12.5 (13 kDa, Protein from Komagataella phaffii)

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EMD-33447

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RNA polymerase II elongation complex transcribing a nucleosome (EC58oct)

Release date: Sept. 7, 2022
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7xtd
Q-score: 0.221

Ehara H, Kujirai T, Shirouzu M, Kurumizaka H, Sekine SI

Science (2022) 377 pp. eabp9466-eabp9466 [ DOI: doi:10.1126/science.abp9466 Pubmed: 35981082 ]

Sample:

Rna polymerase subunit abc23, common to rna polymerases i, ii, and iii (17 kDa, Protein from Komagataella phaffii)
Histone h3.3 (15 kDa, Protein from Homo sapiens)
Rna polymerase subunit abc10-alpha (7 kDa, Protein from Komagataella phaffii)
Spt6 (Complex)
Rnapii-associated chromatin remodeling paf1 complex subunit (62 kDa, Protein from Komagataella phaffii)
Protein that interacts with spt6p and copurifies with spt5p and rna polymerase ii (46 kDa, Protein from Komagataella phaffii)
Rna polymerase ii subunit b32 (20 kDa, Protein from Komagataella phaffii)
Rna polymerase ii (Complex from Komagataella phaffii)
Dna (198-mer) (60 kDa, DNA from synthetic construct)
Leo1 (52 kDa, Protein from Komagataella phaffii)
Fact complex subunit (115 kDa, Protein from Komagataella phaffii)
Fact complex subunit pob3 (60 kDa, Protein from Komagataella phaffii)
Rna polymerase ii third largest subunit b44, part of central core (34 kDa, Protein from Komagataella phaffii)
Rna polymerase subunit abc10-beta, common to rna polymerases i, ii, and iii (8 kDa, Protein from Komagataella phaffii)
Rnap ii-associated protein (46 kDa, Protein from Komagataella phaffii)
Elf1,spt4/5,spn1 and paf1c (Complex from Homo sapiens)
Rna (5'-r(p*up*gp*up*ap*ap*up*cp*cp*cp*cp*up*up*gp*gp*cp*gp*gp*up*u)-3') (6 kDa, RNA from synthetic construct)
Magnesium ion (24 Da, Ligand)
Rna polymerase ii elongation complex transcribing a nucleosome (ec58oct) (Complex from Komagataella phaffii)
Dna (198-mer) (61 kDa, DNA from synthetic construct)
Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (24 kDa, Protein from Komagataella phaffii)
Transcription elongation factor spt6 (173 kDa, Protein from Komagataella phaffii)
Component of the paf1p complex (124 kDa, Protein from Komagataella phaffii)
Constituent of paf1 complex with rna polymerase ii, paf1p, hpr1p, ctr9, leo1, rtf1 and ccr4p (44 kDa, Protein from Komagataella phaffii)
Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (16 kDa, Protein from Komagataella phaffii)
Transcription elongation factor spt5 (101 kDa, Protein from Komagataella phaffii)
Transcription elongation factor spt4 (12 kDa, Protein from Komagataella phaffii)
Histone, fact complex subunit (Complex)
Histone h2a type 1-b/e (14 kDa, Protein from Homo sapiens)
Rna polymerase ii subunit (18 kDa, Protein from Komagataella phaffii)
Zinc ion (65 Da, Ligand)
Dna-directed rna polymerase subunit (194 kDa, Protein from Komagataella phaffii)
Dna-directed rna polymerase subunit (13 kDa, Protein from Komagataella phaffii)
Dna-directed rna polymerase subunit beta (139 kDa, Protein from Komagataella phaffii)
Histone h4 (11 kDa, Protein from Homo sapiens)
Histone h2b type 1-j (14 kDa, Protein from Homo sapiens)
Transcription elongation factor 1 homolog (12 kDa, Protein from Komagataella phaffii)
Dna,rna (Complex from Komagataella phaffii)
Rna polymerase ii subunit b12.5 (13 kDa, Protein from Komagataella phaffii)

[Show more]

Sort by: Download

Showing 1 - 2 of 2. Page 1 of 1

Display: Statistics

Download

Select database:

EMDB [2]

Format:

Select the script type:

Shell (Unix-based) Batch (Windows-based)

Preview first 10

Field: