Electron Microscopy Data Bank

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RNA polymerase II elongation complex stalled at SHL(-2) of the nucleosome

Release date: Oct. 3, 2018
Resolution: 8.7 Å
EM Method: Single-particle
Fitted PDBs: 6a5r
Q-score: 0.11

Kujirai T, Ehara H, Fujino Y, Shirouzu M, Sekine SI, Kurumizaka H

Science (2018) 362 pp. 595-598 [ DOI: doi:10.1126/science.aau9904 Pubmed: 30287617 ]

Sample:

Rna polymerase subunit abc10-beta, common to rna polymerases i, ii, and iii (8 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
Rna polymerase ii subunit (18 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
Rna polymerase subunit abc27, common to rna polymerases i, ii, and iii (24 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (16 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
Rna polymerase subunit abc10-alpha (7 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
Magnesium ion (24 Da, Ligand)
Rna polymerase subunit abc23, common to rna polymerases i, ii, and iii (17 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
Histone h2b type 1-j (14 kDa, Protein from Homo sapiens)
Rna (5'-r(p*up*cp*up*cp*gp*up*gp*cp*cp*up*g)-3') (3 kDa, RNA from synthetic construct)
Rna polymerase ii elongation complex stalled at shl(-2) of the nucleosome (Complex from Komagataella phaffii)
Histone h3.3 (15 kDa, Protein from Homo sapiens)
Dna (198-mer) (61 kDa, DNA from synthetic construct)
Rna polymerase ii subunit b12.5 (13 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
Histone h2a type 1-b/e (14 kDa, Protein from Homo sapiens)
Dna (198-mer) (60 kDa, DNA from synthetic construct)
Zinc ion (65 Da, Ligand)
Rna polymerase ii third largest subunit b44, part of central core (34 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
Rna polymerase ii subunit b32 (20 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
Dna-directed rna polymerase subunit (194 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
Dna-directed rna polymerase subunit beta (139 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
Histone h4 (11 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase subunit (13 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))

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