CryoEM structure of apo form ZmRDR2 at 3.4 Angstroms resolution
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7w84
Q-score: 0.438
Du X, Yang Z, Ariza AJF, Wang Q, Xie G, Li S, Du J
Plant Cell (2022) 34 pp. 2140-2149 [ DOI: doi:10.1093/plcell/koac067 Pubmed: 35188193 ]
- Rna-dependent rna polymerase 2 in apo form (Cellular component from Zea mays)
- Rna-dependent rna polymerase (126 kDa, Protein from Zea mays)
CryoEM structure of open form ZmRDR2 at 3.5 Angstroms resolution
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7w88
Q-score: 0.422
Du X, Yang Z, Ariza AJF, Wang Q, Xie G, Li S, Du J
Plant Cell (2022) 34 pp. 2140-2149 [ Pubmed: 35188193 DOI: doi:10.1093/plcell/koac067 ]
- Rna-dependent rna polymerase 2 in open form (Cellular component from Zea mays)
- Rna-dependent rna polymerase (126 kDa, Protein from Zea mays)
The cryo-EM structure of A. thaliana Pol IV-RDR2 backtracked complex
Resolution: 3.16 Å
EM Method: Single-particle
Fitted PDBs: 7eu0
Q-score: 0.487
Huang K, Wu XX, Fang CL, Xu ZG, Zhang HW, Gao J, Zhou CM, You LL, Gu ZX, Mu WH, Feng Y, Wang JW, Zhang Y
Science (2021) 374 pp. 1579-1586 [ Pubmed: 34941388 DOI: doi:10.1126/science.abj9184 ]
- Rna-dependent rna polymerase 2 (129 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 6a (16 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii and iv subunit 5a (24 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 3 (35 kDa, Protein from Arabidopsis thaliana)
- A. thaliana pol iv-rdr2 backtracked complex (550 kDa, Complex from Arabidopsis thaliana)
- Dna-directed rna polymerases iv and v subunit 2 (132 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 8b (16 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 12 (5 kDa, Protein from Arabidopsis thaliana)
- Dna (33-mer) (9 kDa, DNA from synthetic construct)
- Dna-directed rna polymerases ii, iv and v subunit 9a (13 kDa, Protein from Arabidopsis thaliana)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerases ii, iv and v subunit 10 (8 kDa, Protein from Arabidopsis thaliana)
- Dna (5'-d(*cp*tp*gp*tp*gp*cp*tp*tp*ap*tp*cp*gp*gp*tp*ap*gp*ap*gp*tp*g)-3') (6 kDa, DNA from synthetic construct)
- Rna (5'-r(*cp*cp*gp*a)-3') (1 kDa, RNA from synthetic construct)
- Zinc ion (65 Da, Ligand)
- Rna (39-mer) (12 kDa, RNA from synthetic construct)
- Dna-directed rna polymerase iv subunit 1 (169 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 11 (13 kDa, Protein from Arabidopsis thaliana)
The cryo-EM structure of A. thaliana Pol IV-RDR2 holoenzyme
Resolution: 3.86 Å
EM Method: Single-particle
Fitted PDBs: 7eu1
Q-score: 0.434
Huang K, Wu XX, Fang CL, Xu ZG, Zhang HW, Gao J, Zhou CM, You LL, Gu ZX, Mu WH, Feng Y, Wang JW, Zhang Y
Science (2021) 374 pp. 1579-1586 [ Pubmed: 34941388 DOI: doi:10.1126/science.abj9184 ]
- Dna-directed rna polymerases ii, iv and v subunit 9a (13 kDa, Protein from Arabidopsis thaliana)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerases ii, iv and v subunit 11 (13 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 8b (16 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 10 (8 kDa, Protein from Arabidopsis thaliana)
- Rna-dependent rna polymerase 2 (129 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 6a (16 kDa, Protein from Arabidopsis thaliana)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerases ii, iv and v subunit 3 (35 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerase iv subunit 1 (169 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 12 (5 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases iv and v subunit 2 (132 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii and iv subunit 5a (24 kDa, Protein from Arabidopsis thaliana)
- A. thaliana pol iv-rdr2 holoenzyme (Complex from Arabidopsis thaliana)
Structure of RNA-dependent RNA polymerase 2 (RDR2) from Arabidopsis thaliana
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7roz
Q-score: 0.427
Fukudome A, Singh J, Mishra V, Reddem E, Martinez-Marquez F, Wenzel S, Yan R, Shiozaki M, Yu Z, Wang JC, Takagi Y, Pikaard CS
PNAS (2021) 118 [ Pubmed: 34903670 DOI: doi:10.1073/pnas.2115899118 ]
- Rna-dependent rna polymerase 2 (133 kDa, Protein from Arabidopsis thaliana)
- Magnesium ion (24 Da, Ligand)
- Rna-dependent rna polymerase 2 (rdr2) (129 kDa, Complex from Arabidopsis thaliana)
Arabidopsis RNA-dependent RNA polymerase 2
Resolution: 3.57 Å
EM Method: Single-particle
Fitted PDBs: 7rqs
Q-score: 0.341
Fukudome A, Singh J, Mishra V, Reddem E, Martinez-Marquez F, Wenzel S, Yan R, Shiozaki M, Yu Z, Wang JC, Takagi Y, Pikaard CS
PNAS (2021) 118 [ Pubmed: 34903670 DOI: doi:10.1073/pnas.2115899118 ]
- Rna-dependent rna polymerase 2 (133 kDa, Protein from Arabidopsis thaliana)
- Rna-dependent rna polymerase 2 (129 kDa, Complex from Arabidopsis thaliana)
- Magnesium ion (24 Da, Ligand)