Yeast U1 snRNP with humanized U1C Zinc-Finger domain
Resolution: 3.49 Å
EM Method: Single-particle
Fitted PDBs: 8w2o
Q-score: 0.417
Chalivendra S, Shi S, Li X, Kuang Z, Giovinazzo J, Zhang L, Rossi J, Wang J, Saviola A, Welty R, Liu S, Vaeth K, Zhou ZH, Hansen K, Taliaferro JM, Zhao R
RNA (2024) [ Pubmed: 38688558 DOI: doi:10.1261/rna.079917.123 ]
- U1 snrna (182 kDa, RNA from Saccharomyces cerevisiae S288C)
- U1 small nuclear ribonucleoprotein c (27 kDa, Protein from Saccharomyces cerevisiae S288C)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Saccharomyces cerevisiae S288C)
- U1 small nuclear ribonucleoprotein 70 kda homolog (34 kDa, Protein from Saccharomyces cerevisiae S288C)
- Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Saccharomyces cerevisiae S288C)
- Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Saccharomyces cerevisiae S288C)
- U1snrnp (Complex from Saccharomyces cerevisiae S288C)
- Pre-mrna-processing factor 39 (74 kDa, Protein from Saccharomyces cerevisiae S288C)
- 56 kda u1 small nuclear ribonucleoprotein component (56 kDa, Protein from Saccharomyces cerevisiae S288C)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Saccharomyces cerevisiae S288C)
- U1 small nuclear ribonucleoprotein component snu71 (5 kDa, Protein from Saccharomyces cerevisiae S288C)
- Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Saccharomyces cerevisiae S288C)
- Protein luc7 (30 kDa, Protein from Saccharomyces cerevisiae S288C)
- Protein nam8 (57 kDa, Protein from Saccharomyces cerevisiae S288C)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Saccharomyces cerevisiae S288C)
- Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Saccharomyces cerevisiae S288C)
- U1 small nuclear ribonucleoprotein a (40 kDa, Protein from Saccharomyces cerevisiae S288C)
- Act1 pre-mrna (59 kDa, RNA from Saccharomyces cerevisiae S288C)
- U1 small nuclear ribonucleoprotein component prp42 (65 kDa, Protein from Saccharomyces cerevisiae S288C)
The U2 part of Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1)
Resolution: 9.0 Å
EM Method: Single-particle
Fitted PDBs: 7oqb
Q-score: 0.133
Zhang Z, Rigo N, Dybkov O, Fourmann JB, Will CL, Kumar V, Urlaub H, Stark H, Luhrmann R
Nature (2021) 596 pp. 296-300 [ Pubmed: 34349264 DOI: doi:10.1038/s41586-021-03789-5 ]
- Act1 pre-mrna (delta-bs-a) (101 kDa, RNA from Baker's yeast)
- Pre-mrna-splicing factor prp21 (33 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Baker's yeast)
- U2 snrna (376 kDa, RNA from Baker's yeast)
- Cold sensitive u2 snrna suppressor 1 (50 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor rse1 (153 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Baker's yeast)
- U2 small nuclear ribonucleoprotein b'' (12 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor rds3 (12 kDa, Protein from Baker's yeast)
- S. cerevisiae spliceosomal pre-a complex (Complex from Saccharomyces cerevisiae)
- Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Baker's yeast)
- U2 snrnp component hsh155 (110 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor prp9 (63 kDa, Protein from Baker's yeast)
- Rds3 complex subunit 10 (9 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor prp11 (31 kDa, Protein from Baker's yeast)
- U2 small nuclear ribonucleoprotein a' (27 kDa, Protein from Baker's yeast)
- Protein hsh49 (24 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Baker's yeast)
- Pre-mrna-processing atp-dependent rna helicase prp5 (96 kDa, Protein from Baker's yeast)
The U1 part of Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1)
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 7oqc
Q-score: 0.243
Zhang Z, Rigo N, Dybkov O, Fourmann JB, Will CL, Kumar V, Urlaub H, Stark H, Luhrmann R
Nature (2021) 596 pp. 296-300 [ Pubmed: 34349264 DOI: doi:10.1038/s41586-021-03789-5 ]
- U1 small nuclear ribonucleoprotein c (27 kDa, Protein from Baker's yeast)
- U1 small nuclear ribonucleoprotein 70 kda homolog (34 kDa, Protein from Baker's yeast)
- S. cerevisiae spliceosomal pre-a complex (Complex from Saccharomyces cerevisiae)
- 56 kda u1 small nuclear ribonucleoprotein component (56 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Baker's yeast)
- U1 small nuclear ribonucleoprotein component prp42 (65 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Baker's yeast)
- Pre-mrna-processing factor 39 (74 kDa, Protein from Baker's yeast)
- U1 snrna (182 kDa, RNA from Baker's yeast)
- Protein nam8 (57 kDa, Protein from Baker's yeast)
- Act1 pre-mrna (delta bs-a),act1 pre-mrna (delta bs-a),act1 pre-mrna (delta bs-a),act1 pre-mrna (delta bs-a),act1 pre-mrna (delta bs-a),act1 pre-mrna (delta bs-a),act1 pre-mrna (delta bs-a),act1 pre-mrna (delta bs-a),act1 pre-mrna (delta bs-a) (111 kDa, RNA from Baker's yeast)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Baker's yeast)
- Protein luc7 (30 kDa, Protein from Baker's yeast)
- U1 small nuclear ribonucleoprotein component snu71 (71 kDa, Protein from Baker's yeast)
- U1 small nuclear ribonucleoprotein a (34 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Baker's yeast)
Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1)
Resolution: 5.9 Å
EM Method: Single-particle
Fitted PDBs: 7oqe
Q-score: 0.121
Zhang Z, Rigo N, Dybkov O, Fourmann JB, Will CL, Kumar V, Urlaub H, Stark H, Luhrmann R
Nature (2021) 596 pp. 296-300 [ Pubmed: 34349264 DOI: doi:10.1038/s41586-021-03789-5 ]
- U1 small nuclear ribonucleoprotein c (27 kDa, Protein from Baker's yeast)
- U1 small nuclear ribonucleoprotein 70 kda homolog (34 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor prp9 (63 kDa, Protein from Baker's yeast)
- S. cerevisiae spliceosomal pre-a complex (Complex from Saccharomyces cerevisiae)
- U2 snrnp component hsh155 (110 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor rse1 (153 kDa, Protein from Baker's yeast)
- 56 kda u1 small nuclear ribonucleoprotein component (56 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor prp21 (33 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Baker's yeast)
- Pre-mrna-processing protein prp40 (69 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Baker's yeast)
- U1 small nuclear ribonucleoprotein component prp42 (65 kDa, Protein from Baker's yeast)
- U2 small nuclear ribonucleoprotein a' (27 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor prp11 (31 kDa, Protein from Baker's yeast)
- Pre-mrna-processing factor 39 (74 kDa, Protein from Baker's yeast)
- U1 snrna (182 kDa, RNA from Baker's yeast)
- Protein nam8 (57 kDa, Protein from Baker's yeast)
- Act1 pre-mrna (delta bs-a) (111 kDa, RNA from Baker's yeast)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor rds3 (12 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Baker's yeast)
- Protein luc7 (30 kDa, Protein from Baker's yeast)
- U1 small nuclear ribonucleoprotein component snu71 (71 kDa, Protein from Baker's yeast)
- U2 small nuclear ribonucleoprotein b'' (12 kDa, Protein from Baker's yeast)
- U1 small nuclear ribonucleoprotein a (34 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Baker's yeast)
- Rds3 complex subunit 10 (10 kDa, Protein from Baker's yeast)
- U2 snrna (376 kDa, RNA from Baker's yeast)
- Pre-mrna-processing atp-dependent rna helicase prp5 (96 kDa, Protein from Baker's yeast)
- Protein hsh49 (24 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Baker's yeast)
- Cold sensitive u2 snrna suppressor 1 (50 kDa, Protein from Baker's yeast)
Yeast C complex spliceosome at 2.8 Angstrom resolution with Prp18/Slu7 bound, composite map
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7b9v
Q-score: 0.378
Wilkinson ME, Fica SM, Galej WP, Nagai K
Mol Cell (2021) 81 pp. 1439- [ Pubmed: 33705709 DOI: doi:10.1016/j.molcel.2021.02.021 ]
- Clf1 isoform 1 (82 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc21 (15 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing helicase brr2 (246 kDa, Protein from Baker's yeast)
- 5' exon of ubc4 mrna (15 kDa, RNA from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor snu114 (114 kDa, Protein from Baker's yeast)
- Syf1 isoform 1 (100 kDa, Protein from Baker's yeast)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Pre-mrna-splicing factor cwc25 (20 kDa, Protein from Baker's yeast)
- Bj4_g0054360.mrna.1.cds.1 (50 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor slt11 (40 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor syf2 (24 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc15 (19 kDa, Protein from Baker's yeast)
- Hlj1_g0053790.mrna.1.cds.1 (27 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Baker's yeast)
- D-chiro inositol hexakisphosphate (660 Da, Ligand)
- Snt309 isoform 1 (20 kDa, Protein from Baker's yeast)
- Pre-mrna-processing factor 19 (56 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor isy1 (27 kDa, Protein from Baker's yeast)
- Potassium ion (39 Da, Ligand)
- Branched intron and 3' exon of ubc4 pre-mrna (30 kDa, RNA from Saccharomyces cerevisiae)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Baker's yeast)
- Zinc ion (65 Da, Ligand)
- Pre-mrna-splicing factor cwc2 (38 kDa, Protein from Baker's yeast)
- Cwc22 isoform 1 (67 kDa, Protein from Baker's yeast)
- Bj4_g0027490.mrna.1.cds.1 (12 kDa, Protein from Baker's yeast)
- Ntc20 isoform 1 (15 kDa, Protein from Baker's yeast)
- Pre-mrna-processing protein 45 (42 kDa, Protein from Baker's yeast)
- Splicing factor yju2 (33 kDa, Protein from Baker's yeast)
- Cdc40 isoform 1 (52 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Baker's yeast)
- Y55_g0042700.mrna.1.cds.1 (67 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor slu7 (44 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor 18 (28 kDa, Protein from Baker's yeast)
- Magnesium ion (24 Da, Ligand)
- Pre-mrna-splicing factor atp-dependent rna helicase prp16 (121 kDa, Protein from Baker's yeast)
- U5 snrna (68 kDa, RNA from Saccharomyces cerevisiae)
- Unassigned structure (20 kDa, Protein from Saccharomyces cerevisiae)
- Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor 8 (279 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Baker's yeast)
- Bud31 isoform 1 (18 kDa, Protein from Baker's yeast)
- U2 snrna (376 kDa, RNA from Saccharomyces cerevisiae)
- Yeast c complex spliceosome with prp18/slu7 bound (2 MDa, Complex from Saccharomyces cerevisiae)
- U6 snrna (35 kDa, RNA from Saccharomyces cerevisiae)
Overall map of a transcribing RNA polymerase II-U1 snRNP complex with 145-nt RNA
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7b0y
Q-score: 0.344
Zhang S, Aibara S, Vos SM, Agafonov DE, Luhrmann R, Cramer P
Science (2021) 371 pp. 305-309 [ DOI: doi:10.1126/science.abf1870 Pubmed: 33446560 ]
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Homo sapiens)
- Template dna (14 kDa, DNA from synthetic construct)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Homo sapiens)
- U1 snrnp (Complex from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb1 (217 kDa, Protein from Sus scrofa domesticus)
- Dna-directed rna polymerase subunit beta (134 kDa, Protein from Sus scrofa domesticus)
- Nucleic acids (Complex from synthetic construct)
- Dna-directed rna polymerase ii subunit rpb9 (14 kDa, Protein from Sus scrofa domesticus)
- Rpb12 (7 kDa, Protein from Sus scrofa domesticus)
- Zinc ion (65 Da, Ligand)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Homo sapiens)
- Rna polymerase ii (Complex from Sus scrofa domesticus)
- Dna-directed rna polymerase ii subunit f (14 kDa, Protein from Sus scrofa domesticus)
- The transcribing rna polymerase ii-u1 snrnp complex with 145-nt rna (Complex)
- Dna-directed rna polymerase ii subunit rpb7 (19 kDa, Protein from Sus scrofa domesticus)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (17 kDa, Protein from Sus scrofa domesticus)
- U1 small nuclear ribonucleoprotein a (31 kDa, Protein from Homo sapiens)
- U1 small nuclear ribonucleoprotein 70 kda (51 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase ii subunit e (24 kDa, Protein from Sus scrofa domesticus)
- Small nuclear ribonucleoprotein-associated protein (23 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit d (16 kDa, Protein from Sus scrofa domesticus)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Homo sapiens)
- U1 snrna (52 kDa, RNA from Homo sapiens)
- Non-template dna (14 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase ii subunit rpb3 (31 kDa, Protein from Sus scrofa domesticus)
- 145-nt rna (46 kDa, RNA from synthetic construct)
- Dna-directed rna polymerase ii subunit rpb11-a (13 kDa, Protein from Sus scrofa domesticus)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (7 kDa, Protein from Sus scrofa domesticus)
Saccharomyces cerevisiae spliceosomal E complex (UBC4)
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6n7p
Q-score: 0.352
Li X, Liu S, Zhang L, Issaian A, Hill RC, Espinosa S, Shi S, Cui Y, Kappel K, Das R, Hansen KC, Zhou ZH, Zhao R
Nature (2019) 573 pp. 375-380 [ DOI: doi:10.1038/s41586-019-1523-6 Pubmed: 31485080 ]
- Protein nam8 (57 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Pre-mrna-processing factor 39 (74 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Protein luc7 (30 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U1 small nuclear ribonucleoprotein c (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U1 small nuclear ribonucleoprotein 70 kda homolog (34 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Nuclear cap-binding protein complex subunit 1 (100 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Ubc4 pre-mrna (81 kDa, RNA from Saccharomyces cerevisiae S288C)
- 56 kda u1 small nuclear ribonucleoprotein component (56 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U1 small nuclear ribonucleoprotein component prp42 (65 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U1 small nuclear ribonucleoprotein a (40 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Saccharomyces cerevisiae spliceosomal e complex (ebc4) (Complex from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Pre-mrna-processing protein prp40 (69 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U1 snrna (182 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Nuclear cap-binding protein subunit 2 (23 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U1 small nuclear ribonucleoprotein component snu71,u1 small nuclear ribonucleoprotein component snu71,snu71 (10 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Saccharomyces cerevisiae spliceosomal E complex (ACT1)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 6n7r
Q-score: 0.427
Li X, Liu S, Zhang L, Issaian A, Hill RC, Espinosa S, Shi S, Cui Y, Kappel K, Das R, Hansen KC, Zhou ZH, Zhao R
Nature (2019) 573 pp. 375-380 [ DOI: doi:10.1038/s41586-019-1523-6 Pubmed: 31485080 ]
- Protein nam8 (57 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Pre-mrna-processing factor 39 (74 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Protein luc7 (30 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Saccharomyces cerevisiae spliceosomal e complex (act1) (Complex from Saccharomyces cerevisiae)
- U1 small nuclear ribonucleoprotein c (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U1 small nuclear ribonucleoprotein 70 kda homolog (34 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 56 kda u1 small nuclear ribonucleoprotein component (56 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U1 small nuclear ribonucleoprotein component prp42 (65 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U1 small nuclear ribonucleoprotein a (40 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Act1 pre-mrna (70 kDa, RNA from Saccharomyces cerevisiae S288C)
- U1 snrna (182 kDa, RNA from Saccharomyces cerevisiae)
- Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Zinc ion (65 Da, Ligand)
- Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U1 small nuclear ribonucleoprotein component snu71,u1 small nuclear ribonucleoprotein component snu71,snu71 (9 kDa, Protein from Saccharomyces cerevisiae S288C)
Cryo-EM structure of the yeast B*-a2 complex at an average resolution of 3.2 angstrom
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 6j6g
Q-score: 0.317
Wan R, Bai R, Yan C, Lei J, Shi Y
Cell (2019) 177 pp. 339-351.e13 [ Pubmed: 30879786 DOI: doi:10.1016/j.cell.2019.02.006 ]
- Act1 pre-mrna (217 kDa, RNA from Baker's yeast)
- Pre-mrna-splicing factor slt11 (40 kDa, Protein from Baker's yeast)
- U2 snrna (376 kDa, RNA from Baker's yeast)
- U2 small nuclear ribonucleoprotein a' (27 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor snt309 (20 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Baker's yeast)
- Pre-mrna-processing protein 45 (42 kDa, Protein from Baker's yeast)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Pre-mrna-splicing factor clf1 (82 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor syf2 (24 kDa, Protein from Baker's yeast)
- Magnesium ion (24 Da, Ligand)
- Pre-mrna-splicing factor snu114 (114 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cef1 (67 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc22 (67 kDa, Protein from Baker's yeast)
- Inositol hexakisphosphate (660 Da, Ligand)
- U2 small nuclear ribonucleoprotein b'' (12 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc15 (19 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor prp46 (50 kDa, Protein from Baker's yeast)
- Pre-mrna-processing factor 19 (56 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc21 (15 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Baker's yeast)
- Zinc ion (65 Da, Ligand)
- Pre-mrna-splicing factor bud31 (18 kDa, Protein from Baker's yeast)
- U6 snrna (35 kDa, RNA from Baker's yeast)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from Baker's yeast)
- Catalytically activated spliceosome b*-a2 complex (Complex from Saccharomyces cerevisiae S288c)
- U5 snrna (68 kDa, RNA from Baker's yeast)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Baker's yeast)
- Pre-mrna-processing factor 17 (52 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor isy1 (28 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor 8 (279 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc2 (38 kDa, Protein from Baker's yeast)
Cryo-EM structure of the yeast B*-a1 complex at an average resolution of 3.6 angstrom
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6j6h
Q-score: 0.283
Wan R, Bai R, Yan C, Lei J, Shi Y
Cell (2019) 177 pp. 339-351.e13 [ Pubmed: 30879786 DOI: doi:10.1016/j.cell.2019.02.006 ]
- Pre-mrna-splicing factor slt11 (40 kDa, Protein from Baker's yeast)
- U5 snrna (68 kDa, RNA from Saccharomyces cerevisiae S288c)
- U2 small nuclear ribonucleoprotein a' (27 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor snt309 (20 kDa, Protein from Baker's yeast)
- Pre-mrna-processing protein 45 (42 kDa, Protein from Baker's yeast)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Pre-mrna-splicing factor clf1 (82 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor syf2 (24 kDa, Protein from Baker's yeast)
- U2 snrna (376 kDa, RNA from Saccharomyces cerevisiae S288c)
- Magnesium ion (24 Da, Ligand)
- Act1 pre-mrna (217 kDa, RNA from Saccharomyces cerevisiae S288c)
- Catalytically activated spliceosome b*-a1 complex (Complex from Saccharomyces cerevisiae S288C)
- Pre-mrna-splicing factor snu114 (114 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cef1 (67 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc22 (67 kDa, Protein from Baker's yeast)
- Inositol hexakisphosphate (660 Da, Ligand)
- U2 small nuclear ribonucleoprotein b'' (12 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc15 (19 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor prp46 (50 kDa, Protein from Baker's yeast)
- Pre-mrna-processing factor 19 (56 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc21 (15 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Baker's yeast)
- Zinc ion (65 Da, Ligand)
- Pre-mrna-splicing factor bud31 (18 kDa, Protein from Baker's yeast)
- U6 snrna (35 kDa, RNA from Baker's yeast)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Baker's yeast)
- Pre-mrna-processing factor 17 (52 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor isy1 (28 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor 8 (279 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc2 (38 kDa, Protein from Baker's yeast)