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sample_name:"Subunit NIAM of NADH:Ubiquinone Oxidoreductase (Complex I)" AND film_detector_model:"GATAN K2 SUMMIT (4K X 4K)" AND author_name:"Grba DN"
A 3.7-angstrom structure of Yarrowia lipolytica complex I with an R121M mutation in NUCM.
Release date: March 10, 2021
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7b0n
Q-score: 0.467
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7b0n
Q-score: 0.467
Hameedi MA, Grba DN, Richardson KH, Jones AJY, Song W, Roessler MM, Wright JJ, Hirst J
J Biol Chem (2021) 296 pp. 100474-100474 [ Pubmed: 33640456 DOI: doi:10.1016/j.jbc.2021.100474 ]
Sample:
- Subunit niam of nadh:ubiquinone oxidoreductase (complex i) (17 kDa, Protein from Yarrowia lipolytica)
- Flavin mononucleotide (456 Da, Ligand)
- Cardiolipin (1 kDa, Ligand)
- Epimerase domain-containing protein (40 kDa, Protein from Yarrowia lipolytica)
- Subunit nidm of nadh:ubiquinone oxidoreductase (complex i) (11 kDa, Protein from Yarrowia lipolytica)
- Nucm protein (52 kDa, Protein from Yarrowia lipolytica)
- Subunit nunm of protein nadh:ubiquinone oxidoreductase (complex i) (15 kDa, Protein from Yarrowia lipolytica)
- Acyl carrier protein (8 kDa, Protein from Yarrowia lipolytica)
- Complex i-b14.7 (18 kDa, Protein from Yarrowia lipolytica)
- Subunit ni9m of protein nadh:ubiquinone oxidoreductase (complex i) (8 kDa, Protein from Yarrowia lipolytica)
- Subunit nuvm of nadh:ubiquinone oxidoreductase (complex i) (7 kDa, Protein from Yarrowia lipolytica)
- ~{s}-[2-[3-[[(2~{r})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{s})-3-oxidanyltetradecanethioate (584 Da, Ligand)
- Subunit nuym of nadh:ubiquinone oxidoreductase (complex i) (18 kDa, Protein from Yarrowia lipolytica)
- Acyl carrier protein (9 kDa, Protein from Yarrowia lipolytica)
- Subunit nuhm of nadh:ubiquinone oxidoreductase (complex i) (27 kDa, Protein from Yarrowia lipolytica)
- Subunit nipm of nadh:ubiquinone oxidoreductase (complex i) (10 kDa, Protein from Yarrowia lipolytica)
- Grim-19 (13 kDa, Protein from Yarrowia lipolytica)
- Subunit nuam of nadh:ubiquinone oxidoreductase (complex i) (79 kDa, Protein from Yarrowia lipolytica)
- Subunit nufm of nadh:ubiquinone oxidoreductase (complex i) (16 kDa, Protein from Yarrowia lipolytica)
- Subunit nb2m of nadh:ubiquinone oxidoreductase (complex i) (6 kDa, Protein from Yarrowia lipolytica)
- Subunit nesm of nadh:ubiquinone oxidoreductase (complex i) (28 kDa, Protein from Yarrowia lipolytica)
- Zf-chcc domain-containing protein (13 kDa, Protein from Yarrowia lipolytica)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Subunit nubm of nadh:ubiquinone oxidoreductase (complex i) (53 kDa, Protein from Yarrowia lipolytica)
- Nadh-ubiquinone oxidoreductase chain 4 (54 kDa, Protein from Yarrowia lipolytica)
- Subunit n7bm of nadh:ubiquinone oxidoreductase (complex i) (16 kDa, Protein from Yarrowia lipolytica)
- Subunit nb5m of nadh:ubiquinone oxidoreductase (complex i) (10 kDa, Protein from Yarrowia lipolytica)
- Subunit nb4m of protein nadh:ubiquinone oxidoreductase (complex i) (14 kDa, Protein from Yarrowia lipolytica)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
- Diundecyl phosphatidyl choline (622 Da, Ligand)
- Subunit ni2m of nadh:ubiquinone oxidoreductase (complex i) (12 kDa, Protein from Yarrowia lipolytica)
- Subunit nuxm of nadh:ubiquinone oxidoreductase (complex i) (18 kDa, Protein from Yarrowia lipolytica)
- Subunit nb8m of nadh:ubiquinone oxidoreductase (complex i) (11 kDa, Protein from Yarrowia lipolytica)
- Subunit nukm of nadh:ubiquinone oxidoreductase (complex i) (23 kDa, Protein from Yarrowia lipolytica)
- Nadh dehydrogenase subunit 2 (53 kDa, Protein from Yarrowia lipolytica)
- Zinc ion (65 Da, Ligand)
- Subunit nuzm of nadh:ubiquinone oxidoreductase (complex i) (19 kDa, Protein from Yarrowia lipolytica)
- Nadh-ubiquinone oxidoreductase chain 6 (20 kDa, Protein from Yarrowia lipolytica)
- Yarrowia lipolytica mitochondrial complex i (nadh:ubiquinone oxidoreductase) with nucm r121m mutation (Complex from Yarrowia lipolytica)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 4l (9 kDa, Protein from Yarrowia lipolytica)
- Subunit nupm of nadh:ubiquinone oxidoreductase (complex i) (19 kDa, Protein from Yarrowia lipolytica)
- Nugm protein (33 kDa, Protein from Yarrowia lipolytica)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 (9 kDa, Protein from Yarrowia lipolytica)
- Nadh-ubiquinone oxidoreductase chain 1 (38 kDa, Protein from Yarrowia lipolytica)
- Subunit nuum of nadh:ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Yarrowia lipolytica)
- Nadh-ubiquinone oxidoreductase chain 5 (73 kDa, Protein from Yarrowia lipolytica)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
- Subunit nuim of protein nadh:ubiquinone oxidoreductase (complex i) (21 kDa, Protein from Yarrowia lipolytica)
- Subunit nebm of protein nadh:ubiquinone oxidoreductase (complex i) [yarrowia lipolytica] (7 kDa, Protein from Yarrowia lipolytica)
- Subunit ni8m of nadh:ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Yarrowia lipolytica)
- Nadh-ubiquinone oxidoreductase chain 3 (14 kDa, Protein from Yarrowia lipolytica)
Structure of Yarrowia lipolytica complex I at 2.7 A
Release date: Aug. 12, 2020
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 6yj4
Q-score: 0.602
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 6yj4
Q-score: 0.602
Nat Struct Mol Biol (2020) 27 pp. 892-900 [ DOI: doi:10.1038/s41594-020-0473-x Pubmed: 32747785 ]
Sample:
- Nugm protein (33 kDa, Protein from Yarrowia lipolytica)
- Subunit nupm of nadh:ubiquinone oxidoreductase (complex i) (19 kDa, Protein from Yarrowia lipolytica)
- Subunit nigm of protein nadh:ubiquinone oxidoreductase (complex i) (10 kDa, Protein from Yarrowia lipolytica)
- Subunit nubm of nadh:ubiquinone oxidoreductase (complex i) (53 kDa, Protein from Yarrowia lipolytica)
- Subunit ni8m of nadh:ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Yarrowia lipolytica)
- Nadh-ubiquinone oxidoreductase chain 3 (14 kDa, Protein from Yarrowia lipolytica)
- Cardiolipin (1 kDa, Ligand)
- Subunit nukm of nadh:ubiquinone oxidoreductase (complex i) (23 kDa, Protein from Yarrowia lipolytica)
- Nadh-ubiquinone oxidoreductase chain 1 (38 kDa, Protein from Yarrowia lipolytica)
- Subunit nb4m of protein nadh:ubiquinone oxidoreductase (complex i) (14 kDa, Protein from Yarrowia lipolytica)
- Acyl carrier protein acpm2 of nadh:ubiquinone oxidoreductase (complex i) (14 kDa, Protein from Yarrowia lipolytica)
- Subunit nuzm of nadh:ubiquinone oxidoreductase (complex i) (19 kDa, Protein from Yarrowia lipolytica)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Subunit nimm of nadh:ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Yarrowia lipolytica)
- Subunit nidm of nadh:ubiquinone oxidoreductase (complex i) (11 kDa, Protein from Yarrowia lipolytica)
- Yarrowia lipolytica mitochondrial complex i (Complex from Yarrowia lipolytica)
- Subunit nuim of nadh:ubiquinone oxidoreductase (complex i) (25 kDa, Protein from Yarrowia lipolytica)
- 1,2-diacyl-sn-glycero-3-phosphoethanolamine (748 Da, Ligand)
- Subunit nuem of nadh:ubiquinone oxidoreductase (complex i) (42 kDa, Protein from Yarrowia lipolytica)
- Entity 10 (Complex from Yarrowia lipolytica (strain CLIB 122 / E 150))
- Diundecyl phosphatidyl choline (622 Da, Ligand)
- Subunit nujm of nadh:ubiquinone oxidoreductase (complex i) (20 kDa, Protein from Yarrowia lipolytica)
- Subunit nb6m of nadh:ubiquinone oxidoreductase (complex i) (14 kDa, Protein from Yarrowia lipolytica)
- Yarrowia lipolytica mitochondrial complex i (nadh:ubiquinone oxidoreductase) (Complex)
- Subunit nebm of nadh:ubiquinone oxidoreductase (complex i) (8 kDa, Protein from Yarrowia lipolytica)
- Nadh-ubiquinone oxidoreductase chain 4 (54 kDa, Protein from Yarrowia lipolytica)
- Subunit niam of nadh:ubiquinone oxidoreductase (complex i) (17 kDa, Protein from Yarrowia lipolytica)
- Nadh-ubiquinone oxidoreductase chain 6 (20 kDa, Protein from Yarrowia lipolytica (strain CLIB 122 / E 150))
- Zinc ion (65 Da, Ligand)
- Subunit ni2m of nadh:ubiquinone oxidoreductase (complex i) (12 kDa, Protein from Yarrowia lipolytica)
- Nucm protein (52 kDa, Protein from Yarrowia lipolytica)
- Subunit nb5m of nadh:ubiquinone oxidoreductase (complex i) (10 kDa, Protein from Yarrowia lipolytica)
- Subunit nuhm of nadh:ubiquinone oxidoreductase (complex i) (27 kDa, Protein from Yarrowia lipolytica)
- Acyl carrier protein acpm1 of nadh:ubiquinone oxidoreductase (complex i) (12 kDa, Protein from Yarrowia lipolytica)
- Subunit nb8m of nadh:ubiquinone oxidoreductase (complex i) (11 kDa, Protein from Yarrowia lipolytica)
- ~{s}-[2-[3-[[(2~{r})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{s})-3-oxidanyltetradecanethioate (584 Da, Ligand)
- Subunit nuym of nadh:ubiquinone oxidoreductase (complex i) (18 kDa, Protein from Yarrowia lipolytica)
- Subunit nuxm of nadh:ubiquinone oxidoreductase (complex i) (18 kDa, Protein from Yarrowia lipolytica)
- Water (18 Da, Ligand)
- Subunit n7bm of nadh:ubiquinone oxidoreductase (complex i) (16 kDa, Protein from Yarrowia lipolytica)
- Subunit ni9m of protein nadh:ubiquinone oxidoreductase (complex i) (8 kDa, Protein from Yarrowia lipolytica)
- Subunit nufm of nadh:ubiquinone oxidoreductase (complex i) (16 kDa, Protein from Yarrowia lipolytica)
- Subunit nipm of nadh:ubiquinone oxidoreductase (complex i) (10 kDa, Protein from Yarrowia lipolytica)
- Iron/sulfur cluster (351 Da, Ligand)
- Subunit nesm of nadh:ubiquinone oxidoreductase (complex i) (28 kDa, Protein from Yarrowia lipolytica)
- Subunit nunm of protein nadh:ubiquinone oxidoreductase (complex i) (15 kDa, Protein from Yarrowia lipolytica)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 5 (73 kDa, Protein from Yarrowia lipolytica)
- Subunit nuam of nadh:ubiquinone oxidoreductase (complex i) (79 kDa, Protein from Yarrowia lipolytica)
- Subunit numm of protein nadh:ubiquinone oxidoreductase (complex i) (15 kDa, Protein from Yarrowia lipolytica)
- Flavin mononucleotide (456 Da, Ligand)
- Subunit nuum of nadh:ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Yarrowia lipolytica)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
- Subunit nb2m of nadh:ubiquinone oxidoreductase (complex i) (6 kDa, Protein from Yarrowia lipolytica)
- Nadh-ubiquinone oxidoreductase chain 4l (9 kDa, Protein from Yarrowia lipolytica)
- Nadh dehydrogenase subunit 2 (53 kDa, Protein from Yarrowia lipolytica)