S.cerevisiae THO complex from endogenous nuclear mRNPs
Bonneau F, Basquin J, Steigenberger B, Schafer T, Schafer IB, Conti E
Genes Dev (2023) 37 pp. 505-517 [ DOI: doi:10.1101/gad.350630.123 Pubmed: 37399331 ]
- Tho complex dimer (Complex from Saccharomyces cerevisiae)
- Tho complex subunit hpr1 (Protein from Saccharomyces cerevisiae BY4741)
- Tho complex subunit mft1 (Protein from Saccharomyces cerevisiae BY4741)
- Protein tex1 (Protein from Saccharomyces cerevisiae BY4741)
- Tho complex subunit 2 (Protein from Saccharomyces cerevisiae BY4741)
- Tho complex subunit thp2 (Protein from Saccharomyces cerevisiae BY4741)
Structure of the human TREX core THO-UAP56 complex (map D)
Resolution: 3.45 Å
EM Method: Single-particle
Fitted PDBs: 7znl
Q-score: 0.069
Pacheco-Fiallos B, Vorlander MK, Riabov-Bassat D, Fin L, O'Reilly FJ, Ayala FI, Schellhaas U, Rappsilber J, Plaschka C
Nature (2023) 616 pp. 828-835 [ DOI: doi:10.1038/s41586-023-05904-0 Pubmed: 37020021 ]
- Tho-uap56 (1 MDa, Complex from Homo sapiens)
- Tho complex subunit 7 homolog (23 kDa, Protein from Homo sapiens)
- Tho complex subunit 5 homolog (78 kDa, Protein from Homo sapiens)
- Tho complex subunit 6 homolog (37 kDa, Protein from Homo sapiens)
- Tho complex subunit 3 (38 kDa, Protein from Homo sapiens)
- Tho complex subunit 1 (75 kDa, Protein from Homo sapiens)
- Spliceosome rna helicase ddx39b (49 kDa, Protein from Homo sapiens)
- Tho complex subunit 2 (183 kDa, Protein from Homo sapiens)
Structure of an endogenous human TREX complex bound to mRNA, composite map
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7znk
Q-score: 0.139
Pacheco-Fiallos B, Vorlander MK, Riabov-Bassat D, Fin L, O'Reilly FJ, Ayala FI, Schellhaas U, Rappsilber J, Plaschka C
Nature (2023) 616 pp. 828-835 [ DOI: doi:10.1038/s41586-023-05904-0 Pubmed: 37020021 ]
- Endogenous human trex-mrna complex (1 MDa, Complex from Homo sapiens)
- Tho complex subunit 3 (38 kDa, Protein from human)
- Tho complex subunit 5 homolog (78 kDa, Protein from human)
- Tho complex subunit 1 (75 kDa, Protein from human)
- Tho complex subunit 6 homolog (37 kDa, Protein from human)
- Tho complex subunit 7 homolog (23 kDa, Protein from human)
- Tho complex subunit 2 (183 kDa, Protein from human)
- Rna (873 Da, RNA from Homo sapiens)
- Tho complex subunit 4 (26 kDa, Protein from human)
- Spliceosome rna helicase ddx39b (49 kDa, Protein from human)
Structure of an ALYREF-exon junction complex hexamer
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 7znj
Q-score: 0.608
Pacheco-Fiallos B, Vorlander MK, Riabov-Bassat D, Fin L, O'Reilly FJ, Ayala FI, Schellhaas U, Rappsilber J, Plaschka C
Nature (2023) 616 pp. 828-835 [ DOI: doi:10.1038/s41586-023-05904-0 Pubmed: 37020021 ]
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Maltose/maltodextrin-binding periplasmic protein,tho complex subunit 4 (57 kDa, Protein from Homo sapiens)
- Rna-binding protein 8a (10 kDa, Protein from Homo sapiens)
- Rna (1 kDa, RNA from Homo sapiens)
- Hexameric complex between the trex subunit alyref and the exon junction complex (650 kDa, Complex from Homo sapiens)
- Protein mago nashi homolog (17 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Eukaryotic initiation factor 4a-iii, n-terminally processed (43 kDa, Protein from Homo sapiens)
Dimer structure of yeast THO complex
Chen C, Tan M, Wu Z, Zhang Y, He F, Lu Y, Li S, Cao M, Li G, Wu J, Cheng H, Lei M
Sci Bull (Beijing) (2021) 66 pp. 2347-2352 [ Pubmed: 36654119 DOI: doi:10.1016/j.scib.2021.08.004 ]
- Thp2p (Protein from Saccharomyces cerevisiae)
- Tex1p (Protein from Saccharomyces cerevisiae)
- Mft1p (Protein from Saccharomyces cerevisiae)
- Tho complex (Complex from Saccharomyces cerevisiae S288C)
- Hpr1p (Protein from Saccharomyces cerevisiae)
- Tho2p (Protein from Saccharomyces cerevisiae)
protomer structure from the dimer of yeast THO complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7v2w
Q-score: 0.479
Chen C, Tan M, Wu Z, Zhang Y, He F, Lu Y, Li S, Cao M, Li G, Wu J, Cheng H, Lei M
Sci Bull (Beijing) (2021) 66 pp. 2347-2352 [ Pubmed: 36654119 DOI: doi:10.1016/j.scib.2021.08.004 ]
- Tho complex (Complex from Saccharomyces cerevisiae S288c)
- Tho complex subunit mft1 (45 kDa, Protein from Saccharomyces cerevisiae S288c)
- Tho complex subunit thp2 (30 kDa, Protein from Saccharomyces cerevisiae S288c)
- Tho complex subunit hpr1 (87 kDa, Protein from Saccharomyces cerevisiae S288c)
- Protein tex1 (47 kDa, Protein from Saccharomyces cerevisiae S288c)
- Tho complex subunit 2 (184 kDa, Protein from Saccharomyces cerevisiae S288c)
cryo-EM structure of yeast THO complex with Sub2
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7v2y
Q-score: 0.431
Chen C, Tan M, Wu Z, Zhang Y, He F, Lu Y, Li S, Cao M, Li G, Wu J, Cheng H, Lei M
Sci Bull (Beijing) (2021) 66 pp. 2347-2352 [ Pubmed: 36654119 DOI: doi:10.1016/j.scib.2021.08.004 ]
- Atp-dependent rna helicase sub2 (50 kDa, Protein from Saccharomyces cerevisiae S288c)
- Tho complex subunit mft1 (45 kDa, Protein from Saccharomyces cerevisiae S288c)
- Tho complex subunit thp2 (30 kDa, Protein from Saccharomyces cerevisiae S288c)
- Tho complex subunit hpr1 (87 kDa, Protein from Saccharomyces cerevisiae S288c)
- Protein tex1 (47 kDa, Protein from Saccharomyces cerevisiae S288c)
- Tho-sub2 complex (Complex from Saccharomyces cerevisiae S288c)
- Tho complex subunit 2 (184 kDa, Protein from Saccharomyces cerevisiae S288c)
- Sub2 (Complex)
- Yeast tho (Complex from Saccharomyces cerevisiae S288c)
Cryo-EM structure of the yeast THO-Sub2 complex
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7luv
Q-score: 0.453
Xie Y, Clarke BP, Kim YJ, Ivey AL, Hill PS, Shi Y, Ren Y
eLife (2021) 10 [ Pubmed: 33787496 DOI: doi:10.7554/eLife.65699 ]
- Tex1 (Complex from Saccharomyces bayanus)
- Atp-dependent rna helicase sub2 (50 kDa, Protein from Saccharomyces cerevisiae)
- Tho complex subunit thp2 (30 kDa, Protein from Saccharomyces cerevisiae)
- Tho complex subunit hpr1 (71 kDa, Protein from Saccharomyces cerevisiae)
- Tho (Complex from Saccharomyces cerevisiae)
- Sub2 (Complex from Saccharomyces cerevisiae)
- Tho complex subunit mft1 (29 kDa, Protein from Saccharomyces cerevisiae)
- Tex1 (41 kDa, Protein from Saccharomyces bayanus)
- Tho-sub2 (Complex)
- Tho complex subunit 2 (138 kDa, Protein from Saccharomyces cerevisiae)
Structure of the human THO - UAP56 complex (Map B)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7apk
Q-score: 0.051
Puhringer T, Hohmann U, Fin L, Pacheco-Fiallos B, Schellhaas U, Brennecke J, Plaschka C
eLife (2020) 9 [ Pubmed: 33191911 DOI: doi:10.7554/eLife.61503 ]
- Tho complex subunit 6 homolog (37 kDa, Protein from Homo sapiens)
- Spliceosome rna helicase ddx39b (51 kDa, Protein from Homo sapiens)
- Tho complex (Complex from Homo sapiens)
- Tho complex subunit 3 (43 kDa, Protein from Homo sapiens)
- Tho complex subunit 7 homolog (23 kDa, Protein from Homo sapiens)
- Tho complex subunit 5 homolog (78 kDa, Protein from Homo sapiens)
- Spliceosome rna helicase ddx39b (Complex from Homo sapiens)
- Tho complex subunit 1 (81 kDa, Protein from Homo sapiens)
- Thoc2 anchor (putative) (3 kDa, Protein from Homo sapiens)
- Tho complex subunit 2 (141 kDa, Protein from Homo sapiens)
- Tho - uap56 (1 MDa, Complex)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7apx
Q-score: 0.437
Schuller SK, Schuller JM, Prabu JR, Baumgartner M, Bonneau F, Basquin J, Conti E
eLife (2020) 9 [ Pubmed: 33191913 DOI: doi:10.7554/eLife.61467 ]
- Yeast tho-sub2 complex (431 MDa, Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Tho complex subunit hpr1 (84 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Protein tex1 (42 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Atp-dependent rna helicase sub2 (45 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Tho complex subunit thp2 (30 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Tho complex subunit mft1 (45 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Tho complex subunit 2,tho2 (186 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))