Electron Microscopy Data Bank

EMBL

was set up in 1974 as Europe’s flagship laboratory for the life sciences – an intergovernmental organisation with more than 80 independent research groups covering the spectrum of molecular biology:

Develop

new instruments and methods

Integrating

life science research in Europe

More about EMBL

Six sites

represent EMBL in Europe.

Barcelona

Tissue biology and disease modelling

Grenoble

Structural biology

Hamburg

Structural biology

Heidelberg

Main laboratory

Hinxton

EMBL-EBI: European Bioinformatics Institute

Rome

Epigenetics and neurobiology

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sample_name:"Ternary complex of EsCas13d(R295A/H300A/R849A/H854A) with crRNA, target RNA and Mg2 "

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EMD-9014

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EsCas13d-crRNA-target RNA ternary complex

Release date: Oct. 3, 2018
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6e9f
Q-score: 0.45

Zhang C, Konermann S, Brideau NJ, Lotfy P, Wu X, Novick SJ, Strutzenberg T, Griffin PR, Hsu PD, Lyumkis D

Cell (2018) 175 pp. 212-223.e17 [ Pubmed: 30241607 DOI: doi:10.1016/j.cell.2018.09.001 ]

Sample:

Rna (27-mer) (8 kDa, RNA from bacterium)
Crrna (52-mer) (16 kDa, RNA from bacterium)
Ternary complex of escas13d(r295a/h300a/r849a/h854a) with crrna, target rna and mg2+ (135 kDa, Complex from unidentified bacterium)
Escas13d (110 kDa, Protein from [Eubacterium] siraeum DSM 15702)
Magnesium ion (24 Da, Ligand)

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