Electron Microscopy Data Bank

EMBL

was set up in 1974 as Europe’s flagship laboratory for the life sciences – an intergovernmental organisation with more than 80 independent research groups covering the spectrum of molecular biology:

Develop

new instruments and methods

Integrating

life science research in Europe

More about EMBL

Six sites

represent EMBL in Europe.

Barcelona

Tissue biology and disease modelling

Grenoble

Structural biology

Hamburg

Structural biology

Heidelberg

Main laboratory

Hinxton

EMBL-EBI: European Bioinformatics Institute

Rome

Epigenetics and neurobiology

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sample_name:"Transcription bubble" AND author_name:"Weber G"

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EMD-3561

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map of the RNA polymerase lambda-based antitermination complex solved by cryo-EM

Release date: May 3, 2017
Resolution: 9.8 Å
EM Method: Single-particle
Fitted PDBs: 5ms0
Q-score: 0.069

Said N, Krupp F, Anedchenko E, Santos KF, Dybkov O, Huang YH, Lee CT, Loll B, Behrmann E, Burger J, Mielke T, Loerke J, Urlaub H, Spahn CMT, Weber G, Wahl MC

Nat Microbiol (2017) 2 pp. 17062-17062 [ Pubmed: 28452979 DOI: doi:10.1038/nmicrobiol.2017.62 ]

Sample:

Antitermination protein n (9 kDa, Protein from Escherichia phage lambda)
Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli K-12)
Magnesium ion (24 Da, Ligand)
Dnaii (11 kDa, DNA from Escherichia coli)
Rna transcription bubble (4 kDa, RNA from Escherichia coli)
Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
Lambdan-dependent antitermination complex (Complex from Escherichia coli)
30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli K-12)
Zinc ion (65 Da, Ligand)
Transcription termination/antitermination protein nusa (55 kDa, Protein from Escherichia coli K-12)
Nascent rna (9 kDa, RNA from Escherichia coli)
Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Escherichia coli K-12)
Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
Dnai (8 kDa, DNA from Escherichia coli)
Transcription antitermination protein nusb (15 kDa, Protein from Escherichia coli K-12)
Transcription termination/antitermination protein nusg (20 kDa, Protein from Escherichia coli K-12)

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