Structure of a human 48S translation initiation complex with eIF4F and eIF4A
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8oz0
Q-score: 0.391
Brito Querido J, Sokabe M, Diaz-Lopez I, Gordiyenko Y, Fraser CS, Ramakrishnan V
Nat Struct Mol Biol (2024) 31 pp. 455-464 [ Pubmed: 38287194 DOI: doi:10.1038/s41594-023-01196-0 ]
- Eukaryotic translation initiation factor 3 subunit i (36 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 3 subunit d (64 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 5 (49 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 3 subunit l (66 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 3 subunit b (92 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s15a (14 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s7 (22 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s9 (22 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 2 subunit 3 (51 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 3 subunit h (39 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s29 (6 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s3a (30 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s4, x isoform (29 kDa, Protein from Homo sapiens)
- Trnaimet (24 kDa, RNA from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Eukaryotic translation initiation factor 3 subunit e (52 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s2 (31 kDa, Protein from Homo sapiens)
- Mrna (67 kDa, RNA from Homo sapiens)
- Eukaryotic translation initiation factor 3 subunit k (25 kDa, Protein from Homo sapiens)
- 40s ribosomal protein sa (32 kDa, Protein from Homo sapiens)
- Receptor of activated protein c kinase 1 (35 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s15 (17 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s6 (28 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 3 subunit f (37 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s30 (6 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s14 (16 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s21 (9 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s3 (26 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s19 (16 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s25 (13 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s12 (14 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s28 (7 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s17 (15 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 3 subunit c (105 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s8 (24 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 3 subunit a (166 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- 40s ribosomal protein s27 (9 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l41 (3 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 4 gamma 1 (155 kDa, Protein from Homo sapiens)
- Small ribosomal subunit protein us13 (17 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s26 (13 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 2 subunit 2 (38 kDa, Protein from Homo sapiens)
- 18s rrna (555 kDa, RNA from Homo sapiens)
- 40s ribosomal protein s16 (16 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s24 (15 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s23 (15 kDa, Protein from Homo sapiens)
- Ubiquitin-40s ribosomal protein s27a (18 kDa, Protein from Homo sapiens)
- Human 48s translation initiation complex (Complex from Homo sapiens)
- Eukaryotic translation initiation factor 1a, x-chromosomal (16 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 3 subunit m (42 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s5 (22 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 3 subunit g (35 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s13 (17 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s10 (18 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s11 (18 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 2 subunit 1 (36 kDa, Protein from Homo sapiens)
- Eukaryotic initiation factor 4a-i (46 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s20 (13 kDa, Protein from Homo sapiens)
MERS-CoV Nsp1 bound to the human 43S pre-initiation complex
Resolution: 2.65 Å
EM Method: Single-particle
Fitted PDBs: 8ppl
Q-score: 0.427
Schubert K, Karousis ED, Ban I, Lapointe CP, Leibundgut M, Baumlin E, Kummerant E, Scaiola A, Schonhut T, Ziegelmuller J, Puglisi JD, Muhlemann O, Ban N
Mol Cell (2023) 83 pp. 3546-3557.e8 [ Pubmed: 37802027 DOI: doi:10.1016/j.molcel.2023.09.002 ]
- Eukaryotic translation initiation factor 3 subunit h (39 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s3a (30 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s21 (9 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s15a (14 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 3 subunit k (25 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s27 (9 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 3 subunit m (42 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 3 subunit a (166 kDa, Protein from Homo sapiens)
- Small ribosomal subunit protein es19 (16 kDa, Protein from Homo sapiens)
- Methionine (149 Da, Ligand)
- 40s ribosomal protein s6 (28 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 3 subunit g (35 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s7 (22 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s16 (16 kDa, Protein from Homo sapiens)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- 60s ribosomal protein l41 (3 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s28 (7 kDa, Protein from Homo sapiens)
- Mers-cov nsp1 - 43s pre-initiation complex (Complex from Homo sapiens)
- 40s ribosomal protein s15 (17 kDa, Protein from Homo sapiens)
- Host translation inhibitor nsp1 (24 kDa, Protein from Middle East respiratory syndrome-related coronavirus)
- 40s ribosomal protein s3 (26 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s20 (13 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s25 (13 kDa, Protein from Homo sapiens)
- Small ribosomal subunit protein us13 (17 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s14 (16 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s26 (13 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 3 subunit l (66 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- 40s ribosomal protein s12 (14 kDa, Protein from Homo sapiens)
- 18s rrna (603 kDa, RNA from Homo sapiens)
- 40s ribosomal protein s24 (15 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 1a, x-chromosomal (16 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s29 (6 kDa, Protein from Homo sapiens)
- Met-trnai(met) (24 kDa, RNA from Homo sapiens)
- 40s ribosomal protein s9 (22 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 2 subunit 1 (36 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s5 (22 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s13 (17 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- 40s ribosomal protein s10 (18 kDa, Protein from Homo sapiens)
- Unknown atom or ion (Ligand)
- 40s ribosomal protein s11 (18 kDa, Protein from Homo sapiens)
- Ubiquitin-like fubi-ribosomal protein es30 fusion protein (14 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Eukaryotic translation initiation factor 1 (12 kDa, Protein from Homo sapiens)
- Receptor of activated protein c kinase 1 (35 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 3 subunit c (105 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 2 subunit 2 (38 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 2 subunit 3 (51 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s2 (31 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s8 (24 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 3 subunit f (37 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s17 (15 kDa, Protein from Homo sapiens)
- Ubiquitin-40s ribosomal protein s27a (18 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s4, x isoform (29 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s23 (15 kDa, Protein from Homo sapiens)
- 40s ribosomal protein sa (32 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 3 subunit d (64 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 3 subunit e (52 kDa, Protein from Homo sapiens)
Resolution: 2.77 Å
EM Method: Single-particle
Fitted PDBs: 7zag
Q-score: 0.56
Kazan R, Bourgeois G, Lazennec-Schurdevin C, Larquet E, Mechulam Y, Coureux PD, Schmitt E
Nucleic Acids Res (2022) 50 pp. 6532-6548 [ DOI: doi:10.1093/nar/gkac490 Pubmed: 35694843 ]
- Met-trnaimet, translation initiation factor 1a and translation initiation factor 5b (Complex from Pyrococcus abyssi GE5)
- Zn-ribbon rna-binding protein involved in translation (7 kDa, Protein from Pyrococcus abyssi GE5)
- Methionine (149 Da, Ligand)
- Translation initiation factor 5b (69 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s8e (14 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s19 (15 kDa, Protein from Pyrococcus abyssi GE5)
- Translation initiation factor 1a (15 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s27e (7 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s14 type z (6 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s10 (11 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s7 (24 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s24e (11 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s5 (26 kDa, Protein from Pyrococcus abyssi GE5)
- Rrna (487 kDa, RNA from Pyrococcus abyssi GE5)
- 30s and 50s ribosomal proteins (Complex from Pyrococcus abyssi GE5)
- 30s ribosomal protein s28e (8 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s27ae (5 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s17 (13 kDa, Protein from Pyrococcus abyssi GE5)
- Mrna (8 kDa, RNA from Pyrococcus abyssi GE5)
- Zinc ion (65 Da, Ligand)
- Mrna (Complex from Pyrococcus abyssi GE5)
- 30s ribosomal protein s2 (23 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s6e (14 kDa, Protein from Pyrococcus abyssi GE5)
- 50s ribosomal protein l7ae (13 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s12 (16 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s8 (14 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s3ae (22 kDa, Protein from Pyrococcus abyssi GE5)
- Complex of 30s ribosomal subunit with initiation factor aif1a, the c-terminal domain of aif5b, mrna and trna (Complex)
- 30s ribosomal protein s17e (8 kDa, Protein from Pyrococcus abyssi GE5)
- Water (18 Da, Ligand)
- 30s ribosomal protein s3 (23 kDa, Protein from Pyrococcus abyssi GE5)
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s9 (15 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s4 (21 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s11 (14 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s13 (16 kDa, Protein from Pyrococcus abyssi GE5)
- 50s ribosomal protein l41e (4 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s19e (17 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s4e (28 kDa, Protein from Pyrococcus abyssi GE5)
- Met-trnaimet (24 kDa, RNA from Pyrococcus abyssi GE5)
- 30s ribosomal protein s15 (18 kDa, Protein from Pyrococcus abyssi GE5)
Cryo-EM structure of a Pyrococcus abyssi 30S bound to Met-initiator tRNA, mRNA, aIF1A and aIF5B
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7zah
Q-score: 0.591
Kazan R, Bourgeois G, Lazennec-Schurdevin C, Larquet E, Mechulam Y, Coureux PD, Schmitt E
Nucleic Acids Res (2022) 50 pp. 6532-6548 [ DOI: doi:10.1093/nar/gkac490 Pubmed: 35694843 ]
- Zn-ribbon rna-binding protein involved in translation (7 kDa, Protein from Pyrococcus abyssi GE5)
- Methionine (149 Da, Ligand)
- Mrna (7 kDa, RNA from Pyrococcus abyssi GE5)
- Translation initiation factor 5b (69 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s8e (14 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s19 (15 kDa, Protein from Pyrococcus abyssi GE5)
- Translation initiation factor 1a (15 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s27e (7 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s14 type z (6 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s28e (7 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s10 (11 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s7 (24 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s24e (11 kDa, Protein from Pyrococcus abyssi GE5)
- Initiation complex with 30s ribosomal subunit,trna, mrna and initiation factors 1a and 5b (Complex)
- 30s ribosomal protein s5 (26 kDa, Protein from Pyrococcus abyssi GE5)
- Trna-met, translation initiation factor 1a and translation initiation factor 5b (Complex from Pyrococcus abyssi GE5)
- 30s and 50s ribosomal proteins (Complex from Pyrococcus abyssi GE5)
- 30s ribosomal protein s27ae (5 kDa, Protein from Pyrococcus abyssi GE5)
- Trna-met (24 kDa, RNA from Pyrococcus abyssi GE5)
- 30s ribosomal protein s17 (13 kDa, Protein from Pyrococcus abyssi GE5)
- Zinc ion (65 Da, Ligand)
- Mrna (Complex from Pyrococcus abyssi GE5)
- 30s ribosomal protein s2 (23 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s6e (14 kDa, Protein from Pyrococcus abyssi GE5)
- 50s ribosomal protein l7ae (13 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s12 (16 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s8 (14 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s3ae (22 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s17e (8 kDa, Protein from Pyrococcus abyssi GE5)
- Water (18 Da, Ligand)
- 30s ribosomal protein s3 (23 kDa, Protein from Pyrococcus abyssi GE5)
- Phosphoaminophosphonic acid-guanylate ester (522 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s9 (15 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s4 (21 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s11 (14 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s13 (16 kDa, Protein from Pyrococcus abyssi GE5)
- 50s ribosomal protein l41e (4 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s19e (17 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s4e (28 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s15 (18 kDa, Protein from Pyrococcus abyssi GE5)
- 16s rrna (487 kDa, RNA from Pyrococcus abyssi GE5)
Structure of the wt IRES and 40S ribosome ternary complex, open conformation. Structure 11(wt)
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7syq
Q-score: 0.396
Brown ZP, Abaeva IS, De S, Hellen CUT, Pestova TV, Frank J
EMBO J (2022) 41 pp. e110581-e110581 [ DOI: doi:10.15252/embj.2022110581 Pubmed: 35822879 ]
- Us15 (17 kDa, Protein from rabbit)
- Es12 (14 kDa, Protein from rabbit)
- Es26 (11 kDa, Protein from rabbit)
- Es28 (7 kDa, Protein from rabbit)
- Es1 (30 kDa, Protein from rabbit)
- Us3 (31 kDa, Protein from rabbit)
- Es7 (47 kDa, Protein from rabbit)
- Us7 (22 kDa, Protein from rabbit)
- Us8 (14 kDa, Protein from rabbit)
- Us10 (13 kDa, Protein from rabbit)
- Es25 (13 kDa, Protein from rabbit)
- Us5 (24 kDa, Protein from rabbit)
- Es21 (9 kDa, Protein from rabbit)
- Es4 (s4 x isoform) (29 kDa, Protein from rabbit)
- Us2 (sa) (32 kDa, Protein from rabbit)
- Es17 (15 kDa, Protein from rabbit)
- El41 (3 kDa, Protein from rabbit)
- Es30 (14 kDa, Protein from rabbit)
- Us14 (6 kDa, Protein from rabbit)
- Es31 (21 kDa, Protein from rabbit)
- Us19 (17 kDa, Protein from rabbit)
- Us17 (18 kDa, Protein from rabbit)
- Es6 (28 kDa, Protein from rabbit)
- Us12 (15 kDa, Protein from rabbit)
- Us13 (17 kDa, Protein from rabbit)
- Zinc ion (65 Da, Ligand)
- Hcv ires (128 kDa, RNA from Hepatitis C virus (isolate 1))
- Es24 (15 kDa, Protein from rabbit)
- 40s ribosomal small subunit with hcv ires (2 MDa, Complex)
- Us4 (22 kDa, Protein from rabbit)
- Eukaryotic translation initiation factor 1a, x-chromosomal (16 kDa, Protein from Homo sapiens)
- 18s rrna (603 kDa, RNA from rabbit)
- Es19 (16 kDa, Protein from rabbit)
- Es27 (9 kDa, Protein from rabbit)
- Us11 (18 kDa, Protein from rabbit)
- Es10 (17 kDa, Protein from rabbit)
- Us9 (19 kDa, Protein from rabbit)
- Es8 (24 kDa, Protein from rabbit)
- Receptor for activated c kinase 1 (rack1) (35 kDa, Protein from rabbit)
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7syr
Q-score: 0.347
Brown ZP, Abaeva IS, De S, Hellen CUT, Pestova TV, Frank J
EMBO J (2022) 41 pp. e110581-e110581 [ DOI: doi:10.15252/embj.2022110581 Pubmed: 35822879 ]
- Us15 (17 kDa, Protein from rabbit)
- Es12 (14 kDa, Protein from rabbit)
- Es26 (11 kDa, Protein from rabbit)
- Es28 (7 kDa, Protein from rabbit)
- Es1 (30 kDa, Protein from rabbit)
- Us3 (31 kDa, Protein from rabbit)
- Es7 (47 kDa, Protein from rabbit)
- Us7 (22 kDa, Protein from rabbit)
- Met-trna-i-met (24 kDa, RNA from rabbit)
- Us8 (14 kDa, Protein from rabbit)
- Us10 (13 kDa, Protein from rabbit)
- Es25 (13 kDa, Protein from rabbit)
- Us5 (24 kDa, Protein from rabbit)
- Es21 (9 kDa, Protein from rabbit)
- Es4 (s4 x isoform) (29 kDa, Protein from rabbit)
- Us2 (sa) (32 kDa, Protein from rabbit)
- Es17 (15 kDa, Protein from rabbit)
- El41 (3 kDa, Protein from rabbit)
- Es30 (14 kDa, Protein from rabbit)
- Us14 (6 kDa, Protein from rabbit)
- Es31 (21 kDa, Protein from rabbit)
- Us19 (17 kDa, Protein from rabbit)
- Us17 (18 kDa, Protein from rabbit)
- Es6 (28 kDa, Protein from rabbit)
- Us12 (15 kDa, Protein from rabbit)
- Us13 (17 kDa, Protein from rabbit)
- Zinc ion (65 Da, Ligand)
- Hcv ires (128 kDa, RNA from Hepatitis C virus (isolate 1))
- Es24 (15 kDa, Protein from rabbit)
- 40s ribosomal small subunit with hcv ires (2 MDa, Complex)
- Us4 (22 kDa, Protein from rabbit)
- Eukaryotic translation initiation factor 1a, x-chromosomal (16 kDa, Protein from Homo sapiens)
- 18s rrna (603 kDa, RNA from rabbit)
- Es19 (16 kDa, Protein from rabbit)
- Es27 (9 kDa, Protein from rabbit)
- Eukaryotic translation initiation factor 2 subunit 1 (36 kDa, Protein from Homo sapiens)
- Us11 (18 kDa, Protein from rabbit)
- Es10 (17 kDa, Protein from rabbit)
- Us9 (19 kDa, Protein from rabbit)
- Es8 (24 kDa, Protein from rabbit)
- Receptor for activated c kinase 1 (rack1) (35 kDa, Protein from rabbit)
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7sys
Q-score: 0.469
Brown ZP, Abaeva IS, De S, Hellen CUT, Pestova TV, Frank J
EMBO J (2022) 41 pp. e110581-e110581 [ DOI: doi:10.15252/embj.2022110581 Pubmed: 35822879 ]
- Us12 (15 kDa, Protein from rabbit)
- Us15 (17 kDa, Protein from rabbit)
- Hcv ires (128 kDa, RNA from Hepatitis C virus (isolate 1))
- Us7 (22 kDa, Protein from rabbit)
- Es7 (47 kDa, Protein from rabbit)
- 40s ribosomal small subunit with hcv ires (2 MDa, Complex)
- Es21 (9 kDa, Protein from rabbit)
- Es8 (24 kDa, Protein from rabbit)
- El41 (3 kDa, Protein from rabbit)
- Us19 (17 kDa, Protein from rabbit)
- 18s rrna (603 kDa, RNA from rabbit)
- Receptor for activated c kinase 1 (rack1) (35 kDa, Protein from rabbit)
- Es4 (s4 x isoform) (29 kDa, Protein from rabbit)
- Us13 (17 kDa, Protein from rabbit)
- Zinc ion (65 Da, Ligand)
- Es12 (14 kDa, Protein from rabbit)
- Us10 (13 kDa, Protein from rabbit)
- Eukaryotic translation initiation factor 1a, x-chromosomal (16 kDa, Protein from Homo sapiens)
- Es1 (30 kDa, Protein from rabbit)
- Us8 (14 kDa, Protein from rabbit)
- Us14 (6 kDa, Protein from rabbit)
- Eukaryotic translation initiation factor 2 subunit 1 (36 kDa, Protein from Homo sapiens)
- Es19 (16 kDa, Protein from rabbit)
- Es26 (11 kDa, Protein from rabbit)
- Us11 (18 kDa, Protein from rabbit)
- Us9 (19 kDa, Protein from rabbit)
- Us17 (18 kDa, Protein from rabbit)
- Es31 (21 kDa, Protein from rabbit)
- Es24 (15 kDa, Protein from rabbit)
- Es28 (7 kDa, Protein from rabbit)
- Es10 (17 kDa, Protein from rabbit)
- Us3 (31 kDa, Protein from rabbit)
- Es30 (14 kDa, Protein from rabbit)
- Us5 (24 kDa, Protein from rabbit)
- Es25 (13 kDa, Protein from rabbit)
- Es6 (28 kDa, Protein from rabbit)
- Es27 (9 kDa, Protein from rabbit)
- Us4 (22 kDa, Protein from rabbit)
- Es17 (15 kDa, Protein from rabbit)
- Met-trna-i-met (24 kDa, RNA from rabbit)
- Us2 (sa) (32 kDa, Protein from rabbit)
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7zki
Q-score: 0.418
Kazan R, Bourgeois G, Lazennec-Schurdevin C, Larquet E, Mechulam Y, Coureux PD, Schmitt E
Nucleic Acids Res (2022) 50 pp. 6532-6548 [ DOI: doi:10.1093/nar/gkac490 Pubmed: 35694843 ]
- Translation initiation factor 1a and probable translation initiation factor if-2 (Complex from Pyrococcus abyssi GE5)
- Trna-met (Complex from Escherichia coli)
- Methionine (149 Da, Ligand)
- Translation initiation factor 5b (69 kDa, Protein from Pyrococcus abyssi GE5)
- Met-trnaimet (24 kDa, RNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Phosphoaminophosphonic acid-guanylate ester (522 Da, Ligand)
- Translation initiation factor 1a (15 kDa, Protein from Pyrococcus abyssi GE5)
- Trna-aif5b-aif1a moiety of a pyrococcus abyssi translation initiation complex with 30s ribosomal subunit,trna, mrna and initiation factors 1a and 5b. (Complex)
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7syv
Q-score: 0.347
Brown ZP, Abaeva IS, De S, Hellen CUT, Pestova TV, Frank J
EMBO J (2022) 41 pp. e110581-e110581 [ DOI: doi:10.15252/embj.2022110581 Pubmed: 35822879 ]
- Us15 (17 kDa, Protein from rabbit)
- Es12 (14 kDa, Protein from rabbit)
- Es26 (11 kDa, Protein from rabbit)
- Es28 (7 kDa, Protein from rabbit)
- Es1 (30 kDa, Protein from rabbit)
- Eukaryotic translation initiation factor 5b (70 kDa, Protein from rabbit)
- Us3 (31 kDa, Protein from rabbit)
- Us7 (22 kDa, Protein from rabbit)
- 40s ribosomal protein s7 (47 kDa, Protein from rabbit)
- Met-trna-i-met (24 kDa, RNA from rabbit)
- 40s ribosomal small subunit with hcv ires (2 MDa, Complex from Oryctolagus cuniculus)
- Us10 (13 kDa, Protein from rabbit)
- Us8 (14 kDa, Protein from rabbit)
- 40s ribosomal protein s24 (15 kDa, Protein from rabbit)
- 40s ribosomal protein s4 (29 kDa, Protein from rabbit)
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Es17 (15 kDa, Protein from rabbit)
- Es30 (14 kDa, Protein from rabbit)
- Us19 (17 kDa, Protein from rabbit)
- Sodium ion (22 Da, Ligand)
- Us17 (18 kDa, Protein from rabbit)
- 60s ribosomal protein l41 (3 kDa, Protein from rabbit)
- Es6 (28 kDa, Protein from rabbit)
- Us12 (15 kDa, Protein from rabbit)
- 40s ribosomal protein s27a (21 kDa, Protein from rabbit)
- 40s ribosomal protein sa (32 kDa, Protein from rabbit)
- Us13 (17 kDa, Protein from rabbit)
- Zinc ion (65 Da, Ligand)
- Rack1 (35 kDa, Protein from rabbit)
- Es29 (6 kDa, Protein from rabbit)
- Hcv ires (128 kDa, RNA from hepatitis C virus genotype 1a)
- Us4 (22 kDa, Protein from rabbit)
- Eukaryotic translation initiation factor 1a, x-chromosomal (16 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s21 (9 kDa, Protein from rabbit)
- 18s rrna (603 kDa, RNA from rabbit)
- Es27 (9 kDa, Protein from rabbit)
- 40s ribosomal protein s2 (24 kDa, Protein from rabbit)
- Es19 (16 kDa, Protein from rabbit)
- Us11 (18 kDa, Protein from rabbit)
- 40s ribosomal protein s25 (13 kDa, Protein from rabbit)
- Es10 (17 kDa, Protein from rabbit)
- Us9 (19 kDa, Protein from rabbit)
- Es8 (24 kDa, Protein from rabbit)
Cryo-EM structure of a Pyrococcus abyssi 30S bound to Met-initiator tRNA, mRNA and aIF1A.
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 7zai
Q-score: 0.587
Kazan R, Bourgeois G, Lazennec-Schurdevin C, Larquet E, Mechulam Y, Coureux PD, Schmitt E
Nucleic Acids Res (2022) 50 pp. 6532-6548 [ DOI: doi:10.1093/nar/gkac490 Pubmed: 35694843 ]
- Zn-ribbon rna-binding protein involved in translation (7 kDa, Protein from Pyrococcus abyssi GE5)
- Methionine (149 Da, Ligand)
- 30s ribosomal protein s8e (14 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s19 (15 kDa, Protein from Pyrococcus abyssi GE5)
- Translation initiation factor 1a (15 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s14 type z (6 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s10 (11 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s7 (24 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal subunit with initiation factor 1a, mrna and initiator trna (Complex from Pyrococcus abyssi GE5)
- 30s ribosomal protein s24e (11 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s5 (26 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s28e (8 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s27ae (5 kDa, Protein from Pyrococcus abyssi GE5)
- Trna-met (24 kDa, RNA from Pyrococcus abyssi GE5)
- 30s ribosomal protein s17 (13 kDa, Protein from Pyrococcus abyssi GE5)
- Mrna (8 kDa, RNA from Pyrococcus abyssi GE5)
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein s2 (23 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s6e (14 kDa, Protein from Pyrococcus abyssi GE5)
- 50s ribosomal protein l7ae (13 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s12 (16 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s8 (14 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s3ae (22 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s17e (8 kDa, Protein from Pyrococcus abyssi GE5)
- Water (18 Da, Ligand)
- 30s ribosomal protein s3 (23 kDa, Protein from Pyrococcus abyssi GE5)
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s9 (15 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s4 (21 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s11 (14 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s13 (16 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s27e (7 kDa, Protein from Pyrococcus abyssi)
- 50s ribosomal protein l41e (4 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s19e (17 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s4e (28 kDa, Protein from Pyrococcus abyssi GE5)
- 30s ribosomal protein s15 (18 kDa, Protein from Pyrococcus abyssi GE5)
- 16s rrna (487 kDa, RNA from Pyrococcus abyssi GE5)