Cryo-EM structure of the yeast B*-b1 complex at an average resolution of 3.86 angstrom
Resolution: 3.86 Å
EM Method: Single-particle
Fitted PDBs: 6j6n
Q-score: 0.248
Wan R, Bai R, Yan C, Lei J, Shi Y
Cell (2019) 177 pp. 339-351.e13 [ Pubmed: 30879786 DOI: doi:10.1016/j.cell.2019.02.006 ]
- Pre-mrna-splicing factor snu114 (114 kDa, Protein from Baker's yeast)
- U2 snrna (376 kDa, RNA from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor cwc21 (15 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor isy1 (28 kDa, Protein from Baker's yeast)
- U2 small nuclear ribonucleoprotein b'' (12 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Baker's yeast)
- Catalytically activated spliceosome b*-b1 complex (Complex from Saccharomyces cerevisiae S288C)
- Pre-mrna-processing protein 45 (42 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc15 (19 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor clf1 (82 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor syf2 (24 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor slt11 (40 kDa, Protein from Baker's yeast)
- Zinc ion (65 Da, Ligand)
- Pre-mrna-splicing factor cef1 (67 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor snt309 (20 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor prp46 (50 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Baker's yeast)
- U5 snrna (68 kDa, RNA from Saccharomyces cerevisiae S288c)
- U6 snrna (35 kDa, RNA from Saccharomyces cerevisiae S288c)
- Ubc4 pre-mrna (78 kDa, RNA from Saccharomyces cerevisiae S288c)
- Inositol hexakisphosphate (660 Da, Ligand)
- Pre-mrna-splicing factor bud31 (18 kDa, Protein from Baker's yeast)
- U2 small nuclear ribonucleoprotein a' (27 kDa, Protein from Baker's yeast)
- Pre-mrna-processing factor 19 (56 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc2 (38 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from Baker's yeast)
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Pre-mrna-splicing factor cwc22 (67 kDa, Protein from Baker's yeast)
- Pre-mrna-processing factor 17 (52 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor 8 (279 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Baker's yeast)