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EMBL

was set up in 1974 as Europe’s flagship laboratory for the life sciences – an intergovernmental organisation with more than 80 independent research groups covering the spectrum of molecular biology:

Research:

perform basic research in molecular biology

Services:

offer vital services to scientists in the member states

Training

scientists, students and visitors at all levels

Transfer

and development of technology

Develop

new instruments and methods

Integrating

life science research in Europe

More about EMBL

Six sites

represent EMBL in Europe.

Barcelona

Tissue biology and disease modelling

Grenoble

Structural biology

Hamburg

Structural biology

Heidelberg

Main laboratory

Hinxton

EMBL-EBI: European Bioinformatics Institute

Rome

Epigenetics and neurobiology

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sample_name:"lambda PR promoter DNA (class 2)" AND author_name:"Sotiris J" AND electron_source:"FIELD EMISSION GUN" AND assembly_molecular_weight:{500000 TO 1000000] AND author_name:"Saecker RM"

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EMD-23893

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Cryo-EM structure of Escherichia coli RNA polymerase bound to lambda PR promoter DNA (class 2)

Release date: Sept. 29, 2021
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7mke
Q-score: 0.412

Saecker RM, Chen J, Chiu CE, Malone B, Sotiris J, Ebrahim M, Yen LY, Eng ET, Darst SA

PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2112877118 Pubmed: 34599106 ]

Sample:

Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
Lambda pr promoter dna (class 2) (Complex from Escherichia virus Lambda)
Rna polymerase (Complex from Escherichia coli)
Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
Rna polymerase sigma factor rpod (70 kDa, Protein from Escherichia coli)
Chapso (631 Da, Ligand)
Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
Template strand of lambda pr dna promoter (27 kDa, DNA from Escherichia virus Lambda)
Magnesium ion (24 Da, Ligand)
Escherichia coli rna polymerase bound to lambda pr promoter dna (Complex)
Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
Zinc ion (65 Da, Ligand)
Nontemplate strand of lambda pr dna promoter (27 kDa, DNA from Escherichia virus Lambda)

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