Yeast tubulin polymerized with GTP in vitro
Resolution: 3.7 Å
EM Method: Helical reconstruction
Fitted PDBs: 5w3f
Q-score: 0.366
Howes SC, Geyer EA, LaFrance B, Zhang R, Kellogg EH, Westermann S, Rice LM, Nogales E
J Cell Biol (2017) 216 pp. 2669-2677 [ DOI: doi:10.1083/jcb.201612195 Pubmed: 28652389 ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin alpha-1 chain (49 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Tubulin beta chain (50 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dynamic microtubule lattice (Complex from Saccharomyces cerevisiae S288c)
Yeast microtubule stabilized with epothilone
Resolution: 4.0 Å
EM Method: Helical reconstruction
Fitted PDBs: 5w3h
Q-score: 0.369
Howes SC, Geyer EA, LaFrance B, Zhang R, Kellogg EH, Westermann S, Rice LM, Nogales E
J Cell Biol (2017) 216 pp. 2669-2677 [ DOI: doi:10.1083/jcb.201612195 Pubmed: 28652389 ]
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Microtubule lattice stabilized with epothilone (Complex from Saccharomyces cerevisiae S288c)
- Epothilone a (493 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Tubulin alpha-1 chain (49 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Tubulin beta chain (50 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Yeast microtubule stabilized with Taxol assembled from mutated tubulin
Resolution: 4.0 Å
EM Method: Helical reconstruction
Fitted PDBs: 5w3j
Q-score: 0.338
Howes SC, Geyer EA, LaFrance B, Zhang R, Kellogg EH, Westermann S, Rice LM, Nogales E
J Cell Biol (2017) 216 pp. 2669-2677 [ DOI: doi:10.1083/jcb.201612195 Pubmed: 28652389 ]
- Taxol (853 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin beta chain (51 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Magnesium ion (24 Da, Ligand)
- Tubulin alpha-1 chain (49 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Yeast microtubule stabilized with taxol assembled from mutated tubulin (Complex from Saccharomyces cerevisiae S288c)
Yeast microtubule assembled using the slowly hydrolyzable analogue GMPCPP
Howes SC, Geyer EA, LaFrance B, Zhang R, Kellogg EH, Westermann S, Rice LM, Nogales E
J Cell Biol (2017) 216 pp. 2669-2677 [ DOI: doi:10.1083/jcb.201612195 Pubmed: 28652389 ]
Yeast microtubule assembled using the slowly hydrolyzable analogue GTPgammaS
Howes SC, Geyer EA, LaFrance B, Zhang R, Kellogg EH, Westermann S, Rice LM, Nogales E
J Cell Biol (2017) 216 pp. 2669-2677 [ DOI: doi:10.1083/jcb.201612195 Pubmed: 28652389 ]