Cryo-EM structure of Torpedo acetylcholine receptor in apo form
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 7smm
Q-score: 0.622
Rahman MM, Basta T, Teng J, Lee M, Worrell BT, Stowell MHB, Hibbs RE
Nat Struct Mol Biol (2022) 29 pp. 386-394 [ DOI: doi:10.1038/s41594-022-00737-3 Pubmed: 35301478 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- (2s)-3-(hexadecanoyloxy)-2-[(9z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate (760 Da, Ligand)
- Acetylcholine receptor subunit gamma (56 kDa, Protein from Pacific electric ray, Torpedo californica)
- Torpedo acetylcholine receptor in apo form (Complex from Tetronarce californica)
- Acetylcholine receptor subunit delta (57 kDa, Protein from Pacific electric ray)
- Acetylcholine receptor subunit alpha (50 kDa, Protein from Pacific electric ray)
- Water (18 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Acetylcholine receptor subunit beta (53 kDa, Protein from Pacific electric ray)
- Nonane (128 Da, Ligand)
Cryo-EM structure of Torpedo acetylcholine receptor in apo form with added cholesterol
Resolution: 2.74 Å
EM Method: Single-particle
Fitted PDBs: 7smq
Q-score: 0.583
Rahman MM, Basta T, Teng J, Lee M, Worrell BT, Stowell MHB, Hibbs RE
Nat Struct Mol Biol (2022) 29 pp. 386-394 [ Pubmed: 35301478 DOI: doi:10.1038/s41594-022-00737-3 ]
- Acetylcholine receptor subunit alpha (50 kDa, Protein from Pacific electric ray)
- Acetylcholine receptor subunit gamma (56 kDa, Protein from Pacific electric ray, Torpedo californica)
- Cholesterol (386 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Acetylcholine receptor subunit beta (53 kDa, Protein from Pacific electric ray)
- Torpedo acetylcholine receptor in apo form plus cholesterol (Complex from Tetronarce californica)
- (2s)-3-(hexadecanoyloxy)-2-[(9z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate (760 Da, Ligand)
- Nonane (128 Da, Ligand)
- Acetylcholine receptor subunit delta (57 kDa, Protein from Pacific electric ray)
Cryo-EM structure of Torpedo acetylcholine receptor in complex with carbachol, desensitized state
Resolution: 2.77 Å
EM Method: Single-particle
Fitted PDBs: 7smr
Q-score: 0.597
Rahman MM, Basta T, Teng J, Lee M, Worrell BT, Stowell MHB, Hibbs RE
Nat Struct Mol Biol (2022) 29 pp. 386-394 [ Pubmed: 35301478 DOI: doi:10.1038/s41594-022-00737-3 ]
- Acetylcholine receptor subunit alpha (50 kDa, Protein from Pacific electric ray)
- Acetylcholine receptor subunit gamma (56 kDa, Protein from Pacific electric ray, Torpedo californica)
- Cholesterol (386 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Acetylcholine receptor subunit beta (53 kDa, Protein from Pacific electric ray)
- 2-[(aminocarbonyl)oxy]-n,n,n-trimethylethanaminium (147 Da, Ligand)
- Torpedo acetylcholine receptor in complex with carbachol, desensitized state (Complex from Tetronarce californica)
- Water (18 Da, Ligand)
- (2s)-3-(hexadecanoyloxy)-2-[(9z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate (760 Da, Ligand)
- Nonane (128 Da, Ligand)
- Acetylcholine receptor subunit delta (57 kDa, Protein from Pacific electric ray)
Cryo-EM structure of Torpedo acetylcholine receptor in complex with d-tubocurarine and carbachol
Resolution: 2.56 Å
EM Method: Single-particle
Fitted PDBs: 7smt
Q-score: 0.604
Rahman MM, Basta T, Teng J, Lee M, Worrell BT, Stowell MHB, Hibbs RE
Nat Struct Mol Biol (2022) 29 pp. 386-394 [ Pubmed: 35301478 DOI: doi:10.1038/s41594-022-00737-3 ]
- Acetylcholine receptor subunit alpha (50 kDa, Protein from Pacific electric ray)
- Acetylcholine receptor subunit gamma (56 kDa, Protein from Pacific electric ray, Torpedo californica)
- Cholesterol (386 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Acetylcholine receptor subunit beta (53 kDa, Protein from Pacific electric ray)
- 2-[(aminocarbonyl)oxy]-n,n,n-trimethylethanaminium (147 Da, Ligand)
- (2s)-3-(hexadecanoyloxy)-2-[(9z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate (760 Da, Ligand)
- Nonane (128 Da, Ligand)
- Acetylcholine receptor subunit delta (57 kDa, Protein from Pacific electric ray)
- Torpedo acetylcholine receptor in complex with d-tubocurarine and carbachol (Complex from Tetronarce californica)
- D-tubocurarine (610 Da, Ligand)
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7sv7
Q-score: 0.463
Cell (2022) 185 pp. 158-168.e11 [ Pubmed: 34995514 DOI: doi:10.1016/j.cell.2021.12.009 ]
- Tetradecane (198 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Cystic fibrosis transmembrane conductance regulator (1 kDa, Protein from Homo sapiens)
- Dodecane (170 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Tezacaftor (520 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Nonane (128 Da, Ligand)
- Cystic fibrosis transmembrane conductance regulator (168 kDa, Protein from Homo sapiens)
- The complex of phosphorylated human cystic fibrosis transmembrane conductance regulator (cftr) with atp/mg and tezacaftor (vx-661) (Complex from Homo sapiens)
- Heptadecane (240 Da, Ligand)