Structure of the RQT-bound 80S ribosome from S. cerevisiae (C2) - composite map
Best K, Ikeuchi K, Kater L, Best D, Musial J, Matsuo Y, Berninghausen O, Becker T, Inada T, Beckmann R
Nat Commun (2023) 14 pp. 921-921 [ Pubmed: 36801861 DOI: doi:10.1038/s41467-023-36230-8 ]
RQT-bound 80S ribosome from S. cerevisiae (C1, raw consensus map)
Best K, Ikeuchi K, Kater L, Best D, Musial J, Matsuo Y, Berninghausen O, Becker T, Inada T, Beckmann R
Nat Commun (2023) 14 pp. 921-921 [ Pubmed: 36801861 DOI: doi:10.1038/s41467-023-36230-8 ]
Structure of the RQT-bound 80S ribosome from S. cerevisiae (C1)
Resolution: 3.47 Å
EM Method: Single-particle
Fitted PDBs: 7zpq
Q-score: 0.522
Best K, Ikeuchi K, Kater L, Best D, Musial J, Matsuo Y, Berninghausen O, Becker T, Inada T, Beckmann R
Nat Commun (2023) 14 pp. 921-921 [ Pubmed: 36801861 DOI: doi:10.1038/s41467-023-36230-8 ]
- 60s ribosomal protein l19-a (21 kDa, Protein from baker's yeast)
- Rqt4 isoform 1 (60 kDa, Protein from Saccharomyces cerevisiae)
- Slh1 isoform 1 (225 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s21-a (9 kDa, Protein from baker's yeast)
- 60s ribosomal protein l30 (10 kDa, Protein from baker's yeast)
- 60s ribosomal protein l31-a (12 kDa, Protein from baker's yeast)
- 60s ribosomal protein l9-a (21 kDa, Protein from baker's yeast)
- 40s ribosomal protein s25 (9 kDa, Protein from baker's yeast)
- 60s ribosomal protein l20-a (20 kDa, Protein from baker's yeast)
- 60s ribosomal protein l15-a (24 kDa, Protein from baker's yeast)
- Rpl5 isoform 1 (33 kDa, Protein from baker's yeast)
- 60s ribosomal protein l33-a (12 kDa, Protein from baker's yeast)
- 60s ribosomal protein l37-a (9 kDa, Protein from baker's yeast)
- Rps5p (22 kDa, Protein from baker's yeast)
- Ribosome (Complex from Saccharomyces cerevisiae)
- 25s ribosomal rna (1 MDa, RNA from Saccharomyces cerevisiae)
- 60s ribosomal protein l29 (6 kDa, Protein from baker's yeast)
- 40s ribosomal protein s11-a (16 kDa, Protein from baker's yeast)
- 60s ribosomal protein l13-a (21 kDa, Protein from baker's yeast)
- 40s ribosomal protein s8 (22 kDa, Protein from baker's yeast)
- 60s ribosomal protein l8-a (25 kDa, Protein from baker's yeast)
- 60s ribosomal protein l14-a (14 kDa, Protein from baker's yeast)
- 40s ribosomal protein s14-b (13 kDa, Protein from baker's yeast)
- 60s ribosomal protein l21-a (18 kDa, Protein from baker's yeast)
- 5.8s ribosomal rna (50 kDa, RNA from baker's yeast)
- 60s ribosomal protein l34-a (12 kDa, Protein from baker's yeast)
- 40s ribosomal protein s9-a (21 kDa, Protein from baker's yeast)
- 40s ribosomal protein s16-a (15 kDa, Protein from baker's yeast)
- Rpl41a isoform 1 (3 kDa, Protein from baker's yeast)
- Rpl38 isoform 1 (8 kDa, Protein from baker's yeast)
- 60s ribosomal protein l27-a (15 kDa, Protein from baker's yeast)
- 60s ribosomal protein l17-a (20 kDa, Protein from baker's yeast)
- 18s ribosomal rna (579 kDa, RNA from baker's yeast)
- Rpl24a isoform 1 (14 kDa, Protein from baker's yeast)
- Rpl11b isoform 1 (19 kDa, Protein from baker's yeast)
- Rqt complex (slh1, cue3 and rqt4) (Complex from Saccharomyces cerevisiae)
- 40s ribosomal protein s27-a (8 kDa, Protein from baker's yeast)
- 60s ribosomal protein l16-a (22 kDa, Protein from baker's yeast)
- 40s ribosomal protein s7-a (21 kDa, Protein from baker's yeast)
- Ribosome with bound rqt components (slh1, cue3 and rqt4) (Complex from Saccharomyces cerevisiae)
- 60s ribosomal protein l36-a (11 kDa, Protein from baker's yeast)
- 60s ribosomal protein l22-a (11 kDa, Protein from baker's yeast)
- 60s ribosomal protein l25 (13 kDa, Protein from baker's yeast)
- Rps15 isoform 1 (13 kDa, Protein from baker's yeast)
- Rps3 isoform 1 (24 kDa, Protein from baker's yeast)
- 40s ribosomal protein s23-a (15 kDa, Protein from baker's yeast)
- Rps31 isoform 1 (8 kDa, Protein from baker's yeast)
- 40s ribosomal protein s0-a (22 kDa, Protein from baker's yeast)
- 60s ribosomal protein l39 (6 kDa, Protein from baker's yeast)
- Rpl4a isoform 1 (39 kDa, Protein from baker's yeast)
- 60s ribosomal protein l18-a (20 kDa, Protein from baker's yeast)
- 60s ribosomal protein l42-a (11 kDa, Protein from baker's yeast)
- 60s ribosomal protein l7-a (25 kDa, Protein from baker's yeast)
- Rps2 isoform 1 (23 kDa, Protein from baker's yeast)
- 60s ribosomal protein l26-a (14 kDa, Protein from baker's yeast)
- 40s ribosomal protein s12 (13 kDa, Protein from baker's yeast)
- Magnesium ion (24 Da, Ligand)
- Rps22a isoform 1 (14 kDa, Protein from baker's yeast)
- Rps28a isoform 1 (7 kDa, Protein from baker's yeast)
- 40s ribosomal protein s18-a (16 kDa, Protein from baker's yeast)
- 60s ribosomal protein l40-a (6 kDa, Protein from baker's yeast)
- 60s ribosomal protein l6-b (20 kDa, Protein from baker's yeast)
- Rps20 isoform 1 (11 kDa, Protein from baker's yeast)
- 60s ribosomal protein l2-a (27 kDa, Protein from baker's yeast)
- Guanine nucleotide-binding protein subunit beta-like protein (34 kDa, Protein from baker's yeast)
- 40s ribosomal protein s10-a (11 kDa, Protein from baker's yeast)
- 5s ribosomal rna (38 kDa, RNA from baker's yeast)
- 40s ribosomal protein s6-a (26 kDa, Protein from baker's yeast)
- 40s ribosomal protein s4-a (29 kDa, Protein from baker's yeast)
- Rpl10 isoform 1 (25 kDa, Protein from baker's yeast)
- 60s ribosomal protein l35-a (13 kDa, Protein from baker's yeast)
- Zinc ion (65 Da, Ligand)
- 40s ribosomal protein s17-b (15 kDa, Protein from baker's yeast)
- 60s ribosomal protein l23-a (14 kDa, Protein from baker's yeast)
- 40s ribosomal protein s19-a (15 kDa, Protein from baker's yeast)
- 60s ribosomal protein l28 (16 kDa, Protein from baker's yeast)
- Rpl32 isoform 1 (14 kDa, Protein from baker's yeast)
- Trna (24 kDa, RNA from baker's yeast)
- 60s ribosomal protein l43-a (9 kDa, Protein from baker's yeast)
- Rqc trigger complex subunit cue3 (34 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s13 (16 kDa, Protein from baker's yeast)
- Rps29a isoform 1 (6 kDa, Protein from baker's yeast)
- 60s ribosomal protein l3 (43 kDa, Protein from baker's yeast)
- 40s ribosomal protein s1 (28 kDa, Protein from baker's yeast)
- 40s ribosomal protein s24-a (15 kDa, Protein from baker's yeast)
- 40s ribosomal protein s30-a (6 kDa, Protein from baker's yeast)
- Rps26b isoform 1 (11 kDa, Protein from baker's yeast)
Structure of the RQT-bound 80S ribosome from S. cerevisiae (C2) - composite map
Resolution: 4.8 Å
EM Method: Single-particle
Fitted PDBs: 7zrs
Q-score: 0.49
Best K, Ikeuchi K, Kater L, Best D, Musial J, Matsuo Y, Berninghausen O, Becker T, Inada T, Beckmann R
Nat Commun (2023) 14 pp. 921-921 [ Pubmed: 36801861 DOI: doi:10.1038/s41467-023-36230-8 ]
- Rps2 isoform 1 (23 kDa, Protein from baker's yeast)
- 25s ribosomal rna (1 MDa, RNA from baker's yeast)
- Rpl5 isoform 1 (33 kDa, Protein from baker's yeast)
- 60s ribosomal protein l31-a (12 kDa, Protein from baker's yeast)
- 60s ribosomal protein l25 (13 kDa, Protein from baker's yeast)
- 40s ribosomal protein s7-a (21 kDa, Protein from baker's yeast)
- 60s ribosomal protein l19-a (21 kDa, Protein from baker's yeast)
- Ribosome (Complex from Saccharomyces cerevisiae)
- Rps22a isoform 1 (14 kDa, Protein from baker's yeast)
- 60s ribosomal protein l36-a (11 kDa, Protein from baker's yeast)
- Rpl38 isoform 1 (8 kDa, Protein from baker's yeast)
- 40s ribosomal protein s25 (9 kDa, Protein from baker's yeast)
- 60s ribosomal protein l23-a (14 kDa, Protein from baker's yeast)
- Rps5p (22 kDa, Protein from baker's yeast)
- 40s ribosomal protein s30-a (6 kDa, Protein from baker's yeast)
- Rps31 isoform 1 (8 kDa, Protein from baker's yeast)
- 40s ribosomal protein s10-a (11 kDa, Protein from baker's yeast)
- Rpl4a isoform 1 (39 kDa, Protein from baker's yeast)
- 40s ribosomal protein s12 (13 kDa, Protein from baker's yeast)
- 60s ribosomal protein l28 (16 kDa, Protein from baker's yeast)
- 40s ribosomal protein s14-b (13 kDa, Protein from baker's yeast)
- 60s ribosomal protein l34-a (12 kDa, Protein from baker's yeast)
- 40s ribosomal protein s9-a (21 kDa, Protein from baker's yeast)
- Trna (24 kDa, RNA from baker's yeast)
- 40s ribosomal protein s6-a (26 kDa, Protein from baker's yeast)
- 60s ribosomal protein l30 (10 kDa, Protein from baker's yeast)
- 60s ribosomal protein l20-a (20 kDa, Protein from baker's yeast)
- Rps29a isoform 1 (6 kDa, Protein from baker's yeast)
- 60s ribosomal protein l29 (6 kDa, Protein from baker's yeast)
- Guanine nucleotide-binding protein subunit beta-like protein (34 kDa, Protein from baker's yeast)
- 40s ribosomal protein s1-a (28 kDa, Protein from baker's yeast)
- 40s ribosomal protein s0-a (22 kDa, Protein from baker's yeast)
- 40s ribosomal protein s18-a (16 kDa, Protein from baker's yeast)
- Rpl41a isoform 1 (3 kDa, Protein from baker's yeast)
- 60s ribosomal protein l9-a (21 kDa, Protein from baker's yeast)
- Rpl11b isoform 1 (19 kDa, Protein from baker's yeast)
- 60s ribosomal protein l33-a (12 kDa, Protein from baker's yeast)
- 60s ribosomal protein l40-a (6 kDa, Protein from baker's yeast)
- 60s ribosomal protein l21-a (18 kDa, Protein from baker's yeast)
- 60s ribosomal protein l39 (6 kDa, Protein from baker's yeast)
- 60s ribosomal protein l18-a (20 kDa, Protein from baker's yeast)
- 5.8s ribosomal rna (50 kDa, RNA from baker's yeast)
- 60s ribosomal protein l27-a (15 kDa, Protein from baker's yeast)
- 40s ribosomal protein s27-a (8 kDa, Protein from baker's yeast)
- 60s ribosomal protein l17-a (20 kDa, Protein from baker's yeast)
- Rpl24a isoform 1 (14 kDa, Protein from baker's yeast)
- Rps26b isoform 1 (11 kDa, Protein from baker's yeast)
- 40s ribosomal protein s21-a (9 kDa, Protein from baker's yeast)
- 60s ribosomal protein l42-a (11 kDa, Protein from baker's yeast)
- Rps15 isoform 1 (13 kDa, Protein from baker's yeast)
- Rps28a isoform 1 (7 kDa, Protein from baker's yeast)
- 40s ribosomal protein s4-a (29 kDa, Protein from baker's yeast)
- 60s ribosomal protein l43-a (9 kDa, Protein from baker's yeast)
- 5s ribosomal rna (38 kDa, RNA from baker's yeast)
- 40s ribosomal protein s24-a (15 kDa, Protein from baker's yeast)
- 40s ribosomal protein s19-a (15 kDa, Protein from baker's yeast)
- 40s ribosomal protein s23-a (15 kDa, Protein from baker's yeast)
- 60s ribosomal protein l14-a (14 kDa, Protein from baker's yeast)
- Zinc ion (65 Da, Ligand)
- Rpl10 isoform 1 (25 kDa, Protein from baker's yeast)
- 40s ribosomal protein s16-a (15 kDa, Protein from baker's yeast)
- 60s ribosomal protein l2-a (27 kDa, Protein from baker's yeast)
- 60s ribosomal protein l35-a (13 kDa, Protein from baker's yeast)
- 60s ribosomal protein l8-a (25 kDa, Protein from baker's yeast)
- 18s ribosomal rna (579 kDa, RNA from baker's yeast)
- 60s ribosomal protein l6-b (20 kDa, Protein from baker's yeast)
- 40s ribosomal protein s13 (16 kDa, Protein from baker's yeast)
- 60s ribosomal protein l7-a (25 kDa, Protein from baker's yeast)
- 40s ribosomal protein s17-a (15 kDa, Protein from baker's yeast)
- 40s ribosomal protein s8-a (22 kDa, Protein from baker's yeast)
- 40s ribosomal protein s11-a (16 kDa, Protein from baker's yeast)
- Rps20 isoform 1 (11 kDa, Protein from baker's yeast)
- Magnesium ion (24 Da, Ligand)
- Rqt complex (slh1, cue3 and rqt4) (Complex from Saccharomyces cerevisiae)
- Rqc trigger complex helicase slh1 (225 kDa, Protein from Saccharomyces cerevisiae)
- Rpl32 isoform 1 (14 kDa, Protein from baker's yeast)
- Rqc trigger complex subunit rqt4 (60 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l22-a (11 kDa, Protein from baker's yeast)
- 60s ribosomal protein l26-a (14 kDa, Protein from baker's yeast)
- Ribosome with bound rqt components (slh1, cue3 and rqt4) (Complex)
- Rps3 isoform 1 (24 kDa, Protein from baker's yeast)
- 60s ribosomal protein l15-a (24 kDa, Protein from baker's yeast)
- 60s ribosomal protein l16-a (22 kDa, Protein from baker's yeast)
- 60s ribosomal protein l13-a (21 kDa, Protein from baker's yeast)
- 60s ribosomal protein l37-a (9 kDa, Protein from baker's yeast)
- Rqc trigger complex subunit cue3 (34 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l3 (43 kDa, Protein from baker's yeast)
Structure of the RQT-bound 80S ribosome from S. cerevisiae (C2) - focused Refinement 80S
Best K, Ikeuchi K, Kater L, Best D, Musial J, Matsuo Y, Berninghausen O, Becker T, Inada T, Beckmann R
Nat Commun (2023) 14 pp. 921-921 [ Pubmed: 36801861 DOI: doi:10.1038/s41467-023-36230-8 ]
Structure of the RQT-bound 80S ribosome from S. cerevisiae (C2) - focused Refinement RQT
Best K, Ikeuchi K, Kater L, Best D, Musial J, Matsuo Y, Berninghausen O, Becker T, Inada T, Beckmann R
Nat Commun (2023) 14 pp. 921-921 [ Pubmed: 36801861 DOI: doi:10.1038/s41467-023-36230-8 ]
Structure of the RQT-bound 80S ribosome from S. cerevisiae (C2) - consensus map
Best K, Ikeuchi K, Kater L, Best D, Musial J, Matsuo Y, Berninghausen O, Becker T, Inada T, Beckmann R
Nat Commun (2023) 14 pp. 921-921 [ Pubmed: 36801861 DOI: doi:10.1038/s41467-023-36230-8 ]