EMPIAR-10203

Structure of the Macrobrachium rosenbergii Nodavirus: A new genus within the Nodaviridae?

Entry authors:
Publication:
Structure of the Macrobrachium rosenbergii nodavirus: A new genus within the Nodaviridae?
PLoS Biol 16 (2018)
PMID: 30346944
Experiment type:
EMDB
Related PDB entry:
Related EMDB entry:
.EMD-0129 (3.28Å)
Deposited:
2018-08-02
Released:
2018-10-25
Last modified:
2022-02-08
Dataset size:
130.4 GB
Experimental metadata:
Contains:
micrographs
Image not available

Image set

1. Motion corrected micrographs of Macrobrachium rosenbergii nodavirus virus-like particles
Category:micrographs - single frame
Image format:MRC
No. of images or tilt series:2459
Image size:(3838, 3710)
Pixel type:32 BIT FLOAT
Pixel spacing:(1.06 Å, 1.06 Å)
Details:
.To collect high-resolution data on MrNV VLPs, grids were imaged at the electron bioimaging centre (eBIC), Diamond Light Source (UK) using an FEI Titan Krios operated at 47,170 X magnification. Images were recorded on a Gatan K2 BioQuantum energy-filtered direct detector camera operated in zero-loss imaging mode with a slit width of 20 eV. Five second exposures were recorded in electron counting mode, at a frame-rate of four frames per second and a dose rate of 1.8 electrons/pixel/frame. The pixel size was 1.06 Å/pixel.
Motion correction was performed in Motioncor2.
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