EMPIAR-10380

Single particle cryo electron microscopy of the human serotonin transporter bound with paroxetine and in complex with 8B6 Fab

Entry authors:
Publication:
Chemical and structural investigation of the paroxetine-human serotonin transporter complex
Elife 9 (2020)
PMID: 32618269
Experiment type:
EMDB
Related PDB entry:
Related EMDB entry:
.EMD-21368 (3.3Å)
Deposited:
2020-02-10
Released:
2020-07-01
Last modified:
2022-01-14
Dataset size:
2.7 TB
Dataset DOI:
10.6019/EMPIAR-10380
Experimental metadata:
Download xml
Contains:
micrographs
picked particles
Image not available

Image sets

1. Dataset 1 unaligned multi-frame micrographs of SERT-8B6 Fab complex bound to paroxetine
Category:micrographs - multiframe
Image format:TIFF
No. of images or tilt series:2557
Frames per image:40
Image size:(11520, 8184)
Pixel type:UNSIGNED BYTE
Pixel spacing:(0.324 Å, 0.324 Å)
Details:
.Multiframe movies contain 40 raw frames with a total dose of 54 e-/A^2.
2. Dataset 2 unaligned multi-frame micrographs of SERT-8B6 Fab complex bound to paroxetine
Category:micrographs - multiframe
Image format:TIFF
No. of images or tilt series:4613
Frames per image:40
Image size:(11520, 8184)
Pixel type:UNSIGNED BYTE
Pixel spacing:(0.324 Å, 0.324 Å)
Details:
.Multiframe movies contain 40 raw frames with a total dose of 60 e-/A^2.
3. Aligned micrographs of the SERT-8B6 paroxetine Fab complex bound to paroxetine
Category:micrographs - single frame
Image format:MRC
No. of images or tilt series:7170
Image size:(5760, 4092)
Pixel type:32 BIT FLOAT
Pixel spacing:(0.648 Å, 0.648 Å)
Details:
.Aligned micrographs. Picked particles also follow pattern: data/coord/g3_F_paro_05702_mc_DW_coord.star
4. Particle stack of the SERT-8B6 paroxetine Fab complex bound to paroxetine
Category:picked particles - multiframe - unprocessed
Image format:MRC
No. of images or tilt series:420373
Frames per image:1
Image size:(400, 400)
Pixel type:32 BIT FLOAT
Pixel spacing:(0.648 Å, 0.648 Å)
Details:
.Final refined particle stack and coordinates. Picked particles also follow pattern: data/coord/g3_F_paro_05702_mc_DW_coord.star
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