EMPIAR-10874

Single-Particle CryoEM of mammalian V-ATPase with the TLDc domain protein mEAK7 bound (Various Datasets)

Entry authors:
Tan YZ , Abbas YM , Wu JZ , Wu D, Keon KA , Hesketh GG , Bueler SA, Gingras AC , Robinson CV, Grinstein S , Rubinstein JL 
Publication:
CryoEM of endogenous mammalian V-ATPase interacting with the TLDc protein mEAK-7
Tan YZ , Abbas YM , Wu JZ , Wu D, Keon KA , Hesketh GG , Bueler SA, Gingras AC , Robinson CV, Grinstein S , Rubinstein JL 
Life Sci Alliance 5 (2022)
PMID: 35794005
Experiment type:
EMDB
Related PDB entries:
Related EMDB entries:
Deposited:
2021-11-08
Released:
2022-09-05
Last modified:
2022-09-05
Dataset size:
12.7 TB
Dataset DOI:
10.6019/EMPIAR-10874
Experimental metadata:
Download xml
Contains:
micrographs
picked particles
Image not available

Image sets

1. Unaligned multiframe movies of Pig Kidney V-ATPase bound to mEAK-7 collected using Tundra
Category:micrographs - multiframe
Image format:MRCS
No. of images or tilt series:1957
Frames per image:24
Image size:(4096, 4096)
Pixel type:32 BIT FLOAT
Pixel spacing:(0.7512 Å, 0.7512 Å)
Details:
.Movies collected using Tundra. 24 frames were used, pixel size is 0.7512 Å/pixel, total dose of 40 e-/Å2. Compressed using pbzip2.
2. Aligned and dose-weighted micrographs of Pig Kidney V-ATPase bound to mEAK-7 collected using Tundra
Category:micrographs - single frame
Image format:MRC
No. of images or tilt series:1957
Image size:(4096, 4096)
Pixel type:32 BIT FLOAT
Pixel spacing:(0.7512 Å, 0.7512 Å)
Details:
.Micrographs collected using Tundra. Aligned and dose weighted using UCSF MotionCor2.
3. Polished particles of Pig Kidney V-ATPase bound to mEAK-7 collected using Tundra
Category:picked particles - single frame - processed
Image format:MRCS
No. of images or tilt series:1
Image size:(320, 320)
Pixel type:32 BIT FLOAT
Pixel spacing:(1.461 Å, 1.461 Å)
Details:
.Final particle stack after particle polishing using Relion.
4. Unaligned multiframe movies of Pig Kidney V-ATPase bound to mEAK-7 collected using Titan Krios and Falcon4
Category:micrographs - multiframe
Image format:MRCS
No. of images or tilt series:5142
Frames per image:30
Image size:(4096, 4096)
Pixel type:32 BIT FLOAT
Pixel spacing:(1.3225 Å, 1.3225 Å)
Details:
.Movies collected using Titan Krios and Falcon 4. 30 frames were used, pixel size is 1.3225 Å/pixel, total dose of 36 e-/Å2. Compressed using pbzip2. Last frame should be discarded during processing.
5. Aligned and dose-weighted micrographs of Pig Kidney V-ATPase bound to mEAK-7 collected using Titan Krios and Falcon4
Category:micrographs - single frame
Image format:MRC
No. of images or tilt series:5142
Image size:(4096, 4096)
Pixel type:32 BIT FLOAT
Pixel spacing:(1.3225 Å, 1.3225 Å)
Details:
.Micrographs collected using Titan Krios and Falcon 4. Aligned and dose weighted using UCSF MotionCor2.
6. Polished particles of Pig Kidney V-ATPase bound to mEAK-7 collected using Titan Krios and Falcon4
Category:picked particles - multiframe - processed
Image format:MRCS
No. of images or tilt series:1
Frames per image:186338
Image size:(360, 360)
Pixel type:32 BIT FLOAT
Pixel spacing:(1.3225 Å, 1.3225 Å)
Details:
.Final particle stack after particle polishing using Relion.
7. Unaligned multiframe movies of Pig Kidney V-ATPase bound to mEAK-7deltaCterm collected using Titan Krios and Falcon4
Category:micrographs - multiframe
Image format:MRCS
No. of images or tilt series:2013
Frames per image:30
Image size:(4096, 4096)
Pixel type:32 BIT FLOAT
Pixel spacing:(1.3225 Å, 1.3225 Å)
Details:
.Movies collected using Titan Krios and Falcon 4. 30 frames were used, pixel size is 1.3225 Å/pixel, total dose of 37 e-/Å2. Compressed using pbzip2. Last frame should be discarded during processing.
8. Aligned and dose-weighted micrographs of Pig Kidney V-ATPase bound to mEAK-7deltaCterm collected using Titan Krios and Falcon4
Category:micrographs - single frame
Image format:MRC
No. of images or tilt series:2013
Image size:(4096, 4096)
Pixel type:32 BIT FLOAT
Pixel spacing:(1.3225 Å, 1.3225 Å)
Details:
.Micrographs collected using Titan Krios and Falcon 4. Aligned and dose weighted using UCSF MotionCor2.
9. Polished particles of Pig Kidney V-ATPase bound to mEAK-7deltaCterm collected using Titan Krios and Falcon4
Category:picked particles - single frame - processed
Image format:MRCS
No. of images or tilt series:1
Image size:(4096, 4096)
Pixel type:32 BIT FLOAT
Pixel spacing:(1.3225 Å, 1.3225 Å)
Details:
.Final particle stack after particle polishing using Relion.
10. Unaligned multiframe movies of Pig Kidney V-ATPase bound to mEAK-7 with ATP collected using Titan Krios and Falcon4
Category:micrographs - multiframe
Image format:MRCS
No. of images or tilt series:5498
Frames per image:30
Image size:(4096, 4096)
Pixel type:32 BIT FLOAT
Pixel spacing:(1.3225 Å, 1.3225 Å)
Details:
.Movies collected using Titan Krios and Falcon 4. 30 frames were used, pixel size is 1.3225 Å/pixel, total dose of 44 e-/Å2. Compressed using pbzip2. Last frame should be discarded during processing.
11. Aligned and dose-weighted micrographs of Pig Kidney V-ATPase bound to mEAK-7 with ATP collected using Titan Krios and Falcon4
Category:micrographs - single frame
Image format:MRC
No. of images or tilt series:5498
Image size:(4096, 4096)
Pixel type:32 BIT FLOAT
Pixel spacing:(1.3225 Å, 1.3225 Å)
Details:
.Micrographs collected using Titan Krios and Falcon 4. Aligned and dose weighted using UCSF MotionCor2.
12. Polished particles of Pig Kidney V-ATPase bound to mEAK-7 with ATP collected using Titan Krios and Falcon4
Category:picked particles - single frame - processed
Image format:MRCS
No. of images or tilt series:1
Image size:(4096, 4096)
Pixel type:32 BIT FLOAT
Pixel spacing:(1.3225 Å, 1.3225 Å)
Details:
.Final particle stack after particle polishing using Relion.
13. Unaligned multiframe movies of Pig Kidney V-ATPase bound to mEAK-7 with EDTA/EGTA collected using Titan Krios and Falcon4
Category:micrographs - multiframe
Image format:MRCS
No. of images or tilt series:2468
Frames per image:30
Image size:(4096, 4096)
Pixel type:32 BIT FLOAT
Pixel spacing:(1.3225 Å, 1.3225 Å)
Details:
.Movies collected using Titan Krios and Falcon 4. 30 frames were used, pixel size is 1.3225 Å/pixel, total dose of 41 e-/Å2. Compressed using pbzip2. Last frame should be discarded during processing.
14. Aligned and dose-weighted micrographs of Pig Kidney V-ATPase bound to mEAK-7 with EDTA/EGTA collected using Titan Krios and Falcon4
Category:micrographs - single frame
Image format:MRC
No. of images or tilt series:2468
Image size:(4096, 4096)
Pixel type:32 BIT FLOAT
Pixel spacing:(1.3225 Å, 1.3225 Å)
Details:
.Micrographs collected using Titan Krios and Falcon 4. Aligned and dose weighted using UCSF MotionCor2.
15. Polished particles of Pig Kidney V-ATPase bound to mEAK-7 with EDTA/EGTA collected using Titan Krios and Falcon4
Category:picked particles - single frame - processed
Image format:MRCS
No. of images or tilt series:1
Image size:(360, 360)
Pixel type:32 BIT FLOAT
Pixel spacing:(1.3225 Å, 1.3225 Å)
Details:
.Final particle stack after particle polishing using Relion.
16. Unaligned multiframe movies of Pig Kidney V-ATPase bound to mEAK-7 with Calcium collected using Glacios with Selectris X and Falcon 4
Category:micrographs - multiframe
Image format:EER
No. of images or tilt series:3029
Frames per image:1
Image size:(4096, 4096)
Pixel type:32 BIT FLOAT
Pixel spacing:(0.895 Å, 0.895 Å)
Details:
.Movies collected using Glacios with Selectris X and Falcon 4. EER format was used, pixel size is 0.895 Å/pixel, total dose of 40 e-/Å2.
17. Aligned and dose-weighted micrographs of Pig Kidney V-ATPase bound to mEAK-7 with Calcium collected using Glacios with Selectris X and Falcon 4
Category:micrographs - single frame
Image format:MRC
No. of images or tilt series:3029
Image size:(4096, 4096)
Pixel type:32 BIT FLOAT
Pixel spacing:(0.895 Å, 0.895 Å)
Details:
.Micrographs collected using Titan Krios and Falcon 4. Aligned and dose weighted using UCSF MotionCor2.
18. Polished particles of Pig Kidney V-ATPase bound to mEAK-7 with Calcium collected using Glacios with Selectris X and Falcon 4
Category:picked particles - single frame - processed
Image format:MRCS
No. of images or tilt series:1
Image size:(320, 320)
Pixel type:32 BIT FLOAT
Pixel spacing:(1.56625 Å, 1.56625 Å)
Details:
.Final particle stack after particle polishing using Relion.
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