EMPIAR-11070

Tilt series of SARS-CoV-2 virions, Wuhan/Alpha/Beta/Delta variants

Entry authors:
Calder LJ, Calcraft T, Hussain S, Harvey R, Rosenthal PB
Publication:
Electron cryotomography of SARS-CoV-2 virions reveals cylinder-shaped particles with a double layer RNP assembly
Calder LJ, Calcraft T, Hussain S, Harvey R, Rosenthal PB
Communications Biology 5 (2022)
Experiment type:
EMDB
Related EMDB entries:
Deposited:
2022-06-15
Released:
2022-11-11
Last modified:
2022-11-11
Dataset size:
120.5 GB
Experimental metadata:
Contains:
micrographs
tilt series
Image not available

Image sets

1. Unaligned tilt movies of SARS-CoV-2 virions (Wuhan strain)
Category:micrographs - multiframe
Image format:MRC
No. of images or tilt series:533
Frames per image:4
Image size:(3838, 3710)
Pixel type:32 BIT FLOAT
Pixel spacing:(2.22 Å, 2.22 Å)
Details:
.Movies have 4 frames each, corresponding to 2.38e-/A^2 per tilt. Tilt series collected +/-60deg in dose symmetric sequence, grouping 2 (0,+3,-3,-6,+6,+9,etc...). Tilt axis rotation angle is 177deg.
2. Tilt series of SARS-CoV-2 virions (Alpha variant B.1.1.7)
Category:tilt series
Image format:MRC
No. of images or tilt series:2
Image size:(4096, 4096)
Pixel type:UNSIGNED 16 BIT INTEGER
Pixel spacing:(2.58 Å, 2.58 Å)
Details:
.2.5e-/A^2 dose per tilt. Tilt series collected +/-57deg in dose symmetric sequence, grouping 2 (0,+3,-3,-6,+6,+9,etc...). Tilt axis rotation angle is -2deg.
3. Tilt series of SARS-CoV-2 virions (Beta variant B.1.351)
Category:tilt series
Image format:MRC
No. of images or tilt series:2
Image size:(4096, 4096)
Pixel type:UNSIGNED 16 BIT INTEGER
Pixel spacing:(2.58 Å, 2.58 Å)
Details:
.2.5e-/A^2 dose per tilt. Tilt series collected +/-57deg in dose symmetric sequence, grouping 2 (0,+3,-3,-6,+6,+9,etc...). Tilt axis rotation angle is -2deg.
4. Tilt series of SARS-CoV-2 virions (Delta variant B.1.617)
Category:tilt series
Image format:MRC
No. of images or tilt series:2
Image size:(4096, 4096)
Pixel type:UNSIGNED 16 BIT INTEGER
Pixel spacing:(2.58 Å, 2.58 Å)
Details:
.2.0e-/A^2 dose per tilt. Tilt series collected +/-60deg in dose symmetric sequence, grouping 2 (0,+3,-3,-6,+6,+9,etc...). Tilt axis rotation angle is -2deg.
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