EMPIAR-11481

Movies of GroEL-ES-nucleotide complex plunged 13, 50, 200 ms and 20 s after mixing with ATP

Entry authors:
Publication:
Time-resolved cryo-EM using a combination of droplet microfluidics with on-demand jetting
Nature Methods
Experiment type:
EMDB
Related PDB entries:
Related EMDB entries:
.EMD-16157 (6.3Å), EMD-16107 (4.4Å), EMD-16125 (2.5Å), EMD-16116 (3.4Å), EMD-16100 (2.3Å), EMD-16102 (4.4Å), EMD-16119 (3.4Å), EMD-16108 (4.0Å), EMD-16099 (2.3Å), EMD-16109 (3.9Å)
Deposited:
2023-02-08
Released:
2023-08-18
Last modified:
2023-08-18
Dataset size:
2.9 TB
Dataset DOI:
10.6019/EMPIAR-11481
Funding:
.
1. European Research Council (ERC)
726436
Belgium
2. Research Foundation - Flanders (FWO)
G0H5916N
Belgium
3. Research Foundation - Flanders (FWO)
G054617N
Belgium
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Experimental metadata:
Download xml
Contains:
micrographs
Image not available

Image sets

1. GroELES with 20s of mixing time
Category:micrographs - multiframe
Image format:TIFF
No. of images or tilt series:4194
Frames per image:60
Image size:(5760, 4092)
Pixel type:UNSIGNED BYTE
Details:
.STAR files associated with the reconstructed maps from this dataset are uploaded in the directory 'associated_star_files' with names 20sGroEL-ATP.star, 20sGroEL-ATP-GroES.star, 20sGroEL-ATP-ADP-GroES.star and 20s_GroEL-ATP-2GroES.star

gain files are included in the Gain file directory under particular time point. Please use the corresponding gain file by matching the gain file date and micrograph date.
The dates are in format YYYYMMDD
The date is indicated at the start of micrograph name e.g 20220628_trGroEL...tif
The date is the second part of gain refence file name e.g CountRef_20220628_trGroEL...mrc
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2. GroELES at 50ms timepoint
Category:micrographs - multiframe
Image format:TIFF
No. of images or tilt series:1416
Frames per image:60
Image size:(5760, 4092)
Pixel type:UNSIGNED BYTE
Details:
.STAR files associated with the reconstructed maps from this dataset are uploaded in the directory 'associated_star_files' with names 50ms_GroEL-ADP.star, 50ms_GroEL-ATP-ADP.star, 50ms_GroEL-ATP.star

gain files are included in the Gain file directory under particular time point. Please use the corresponding gain file by matching the gain file date and micrograph date.
The dates are in format YYYYMMDD
The date is indicated at the start of micrograph name e.g 20220628_trGroEL...tif
The date is the second part of gain refence file name e.g CountRef_20220628_trGroEL...mrcGroEL was mixed with GroES and ATP and plunged within 50ms
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3. GroEL 13ms timepoint
Category:micrographs - multiframe
Image format:TIFF
No. of images or tilt series:2609
Frames per image:60
Image size:(5760, 4092)
Pixel type:UNSIGNED BYTE
Details:
.STAR files associated with the reconstructed maps from this dataset are uploaded in the directory 'associated_star_files' with names 13ms_GroEL-ADP.star, 13ms-GroEL-ATP-ADP.star, 13ms_GroEL-ATP.star

gain files are included in the Gain file directory under particular time point. Please use the corresponding gain file by matching the gain file date and micrograph date.
The dates are in format YYYYMMDD
The date is indicated at the start of micrograph name e.g 20220628_trGroEL...tif
The date is the second part of gain refence file name e.g CountRef_20220628_trGroEL...mrc
Show more
4. GroELES 200ms timepoint
Category:micrographs - multiframe
Image format:TIFF
No. of images or tilt series:5445
Frames per image:60
Image size:(5760, 4092)
Pixel type:UNSIGNED BYTE
Details:
.STAR files associated with the reconstructed maps from this dataset are uploaded in the directory 'associated_star_files' with names 200msGroEL-ATP.star, 200msGroEL-ATP-GroES.star, 200msGroEL-ATP-ADP-GroES.star and 200ms_GroEL-ATP-2GroES.star

gain files are included in the Gain file directory under particular time point. Please use the corresponding gain file by matching the gain file date and micrograph date.
The dates are in format YYYYMMDD
The date is indicated at the start of micrograph name e.g 20220628_trGroEL...tif
The date is the second part of gain refence file name e.g CountRef_20220628_trGroEL...mrc
Show more
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