EMPIAR-12228

Cryo-EM images of B. megaterium gas vesicles in thin vitreous monolayers

Entry authors:
Publication:
Cryo-EM structure of gas vesicles for buoyancy-controlled motility
Huber ST , Terwiel D, Evers WH, Maresca D, Jakobi AJ 
Cell 186 975-986.e13 (2023)
PMID: 36868215
Experiment type:
EMDB
Related PDB entry:
Related EMDB entry:
.EMD-14238 (3.2Å)
Deposited:
2024-07-26
Released:
2025-02-03
Last modified:
2025-02-03
Dataset size:
2.3 TB
Experimental metadata:
Contains:
micrographs
Image not available

Image set

1. Unaligned multi-frame movies of B.megaterium gas vesicles
Category:micrographs - multiframe
Image format:TIFF
No. of images or tilt series:4352
Frames per image:60
Image size:(11520, 8184)
Pixel type:SIGNED BYTE
Pixel spacing:(0.685 Å, 0.685 Å)
Details:
.Unaligned multi-frame movies of B.megaterium gas vesicles (concentation: OD500=3.1) in superresolution mode from a K3 detector. Pixelsize is 1.37A, superres pixelsize is 0.685A. This pixel size was used for processing and in the publication. Subsequent work established that the pixelsize was not perfectly calibrated and should be closer to 1.341A.
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