› 1bxi

X-ray diffraction
2.05Å resolution

CRYSTAL STRUCTURE OF THE ESCHERICHIA COLI COLICIN E9 DNASE DOMAIN WITH ITS COGNATE IMMUNITY PROTEIN IM9

Released:
DOI: 10.2210/pdb1bxi/pdb
PDB entry 1bxi coloured by chain, front view.
PDB entry 1bxi coloured by chain, side view.
PDB entry 1bxi coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal Structure of the E.Coli Colicin E9 DNase Domain With its Cognate Immunity Protein Im9
Kuhlmann UC
Thesis (1998)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140796 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Colicin-E9 immunity protein Chain: A
Molecule details ›
Chain: A
Length: 86 amino acids
Theoretical weight: 9.52 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P13479 (Residues: 1-86; Coverage: 100%)
Gene names: ceiE9, imm
Sequence domains: Colicin immunity protein / pyocin immunity protein
Structure domains: Colicin E immunity protein
Colicin-E9 Chain: B
Molecule details ›
Chain: B
Length: 134 amino acids
Theoretical weight: 15.21 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P09883 (Residues: 449-582; Coverage: 23%)
Gene names: cei, col
Sequence domains: Colicin/Pyocin-S2, DNase domain
Structure domains: Colicin/pyocin, DNase domain

Ligands and Environments

2 bound ligands:
Ligand NI 1 x NI
Ligand PO4 1 x PO4
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X31
Spacegroup: P212121
Unit cell:
a: 43.85Å b: 51.96Å c: 87.59Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.218 0.315
Expression system: Escherichia coli

Quick links

Citations

27 mentions without citation

Colicin biology.
Cascales et al. (2007)
26 more