› 1j53

X-ray diffraction
1.8Å resolution

Structure of the N-terminal Exonuclease Domain of the Epsilon Subunit of E.coli DNA Polymerase III at pH 8.5

Released:
DOI: 10.2210/pdb1j53/pdb
PDB entry 1j53 coloured by chain, front view.
PDB entry 1j53 coloured by chain, side view.
PDB entry 1j53 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural basis for proofreading during replication of the Escherichia coli chromosome.
Hamdan S, Carr PD, Brown SE, Ollis DL, Dixon NE
Structure 10 535-46 (2002)
PMID: 11937058

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136454 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase III subunit epsilon Chain: A
Molecule details ›
Chain: A
Length: 186 amino acids
Theoretical weight: 20.74 KDa
Source organism: Escherichia coli
Expression system: Escherichia phage T7
UniProt:
  • Canonical: P03007 (Residues: 1-186; Coverage: 77%)
Gene names: JW0205, b0215, dnaQ, mutD
Sequence domains: Exonuclease
Structure domains: Ribonuclease H-like superfamily/Ribonuclease H

Ligands and Environments

3 bound ligands:
Ligand MN 2 x MN
Ligand TMP 2 x TMP
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P41212
Unit cell:
a: 60.8Å b: 60.8Å c: 111.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.199 0.227
Expression system: Escherichia phage T7

Quick links

Citations

14 review citations

Cellular DNA replicases: components and dynamics at the replication fork.
Johnson et al. (2005)
13 more

18 mentions without citation

Architecture and conservation of the bacterial DNA replication machinery, an underexploited drug target.
Robinson et al. (2012)
17 more