› 1pm1

X-ray diffraction
1.1Å resolution

Crystal structure of nitrophorin 2 L122V/L132V mutant complex with imidazole

Released:
DOI: 10.2210/pdb1pm1/pdb
PDB entry 1pm1 coloured by chain, front view.
PDB entry 1pm1 coloured by chain, side view.
PDB entry 1pm1 coloured by chain, top view.
Source organism: Rhodnius prolixus
Entry authors: Weichsel A, Montfort WR

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173241 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrophorin-2 Chain: X
Molecule details ›
Chain: X
Length: 180 amino acids
Theoretical weight: 20.05 KDa
Source organism: Rhodnius prolixus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q26241 (Residues: 24-202; Coverage: 100%)
Sequence domains: Nitrophorin
Structure domains: Lipocalin

Ligands and Environments

2 bound ligands:
Ligand HEM 1 x HEM
Ligand IMD 1 x IMD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P21212
Unit cell:
a: 39.31Å b: 125.47Å c: 33.88Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.155 0.154 0.177
Expression system: Escherichia coli BL21(DE3)

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