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Crystal structure of nitrophorin 2 L122V/L132V mutant complex with imidazole

X-ray diffraction
1.1Å resolution
PDB entry 1pm1 coloured by chain, front view.
PDB entry 1pm1 coloured by chain, side view.
PDB entry 1pm1 coloured by chain, top view.
Source organism: Rhodnius prolixus
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Nitrophorin-2
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Multimeric state: monomeric
Accessible surface area: 9227.83 Å2
Buried surface area: 1410.27 Å2
Dissociation area: 157.34 Å2
Dissociation energy (ΔGdiss): -2.52 kcal/mol
Dissociation entropy (TΔSdiss): -0.17 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-173241

Macromolecules

Nitrophorin-2
Chain: X
Length: 180 amino acids
Theoretical weight: 20.05 KDa
Source organism: Rhodnius prolixus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q26241 (Residues: 24-202; Coverage: 100%)
Pfam: Nitrophorin
InterPro:
CATH: Lipocalin
SCOP: Retinol binding protein-like
Nitrophorin-2 in PDB entry 1pm1, assembly 1, front view.
Nitrophorin-2 in PDB entry 1pm1, assembly 1, side view.
Nitrophorin-2 in PDB entry 1pm1, assembly 1, top view.
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