› 1pzv

X-ray diffraction
2.52Å resolution

Crystal structures of two UBC (E2) enzymes of the ubiquitin-conjugating system in Caenorhabditis elegans

Released:
DOI: 10.2210/pdb1pzv/pdb
Model geometry
Fit model/data
PDB entry 1pzv coloured by chain, front view.
PDB entry 1pzv coloured by chain, side view.
PDB entry 1pzv coloured by chain, top view.
Source organism: Caenorhabditis elegans
Entry authors: Schormann N, Lin G, Li S, Symersky J, Qiu S, Finley J, Luo D, Stanton A, Carson M, Luo M, Southeast Collaboratory for Structural Genomics (SECSG)

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152707 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable ubiquitin-conjugating enzyme E2 7 Chain: A
Molecule details ›
Chain: A
Length: 164 amino acids
Theoretical weight: 18.96 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P34477 (Residues: 1-164; Coverage: 100%)
Gene names: F58A4.10, ubc-7
Sequence domains: Ubiquitin-conjugating enzyme
Structure domains: Ubiquitin Conjugating Enzyme

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 48.474Å b: 52.184Å c: 68.882Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.252 0.241 0.289
Expression system: Escherichia coli BL21(DE3)

Quick links

PDB-REDO

The sliders below show the change in model quality between original PDB entry and the PDB-REDO entry

Model Geometry
 
 
Data not available
DNA/RNA Geometry
 
 
Data not available
Fit model/data
 
 
Data not available
PDB-REDO