› 1tef

X-ray diffraction
1.9Å resolution

Crystal structure of the spinach plastocyanin mutants G8D/K30C/T69C and K30C/T69C- a study of the effect on crystal packing and thermostability from the introduction of a novel disulfide bond

Released:
DOI: 10.2210/pdb1tef/pdb
PDB entry 1tef coloured by chain, front view.
PDB entry 1tef coloured by chain, side view.
PDB entry 1tef coloured by chain, top view.
Source organism: Spinacia oleracea
Entry authors: Okvist M, Jacobson F, Jansson H, Hansson O, Sjolin L

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132377 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Plastocyanin, chloroplastic Chains: A, B
Molecule details ›
Chains: A, B
Length: 99 amino acids
Theoretical weight: 10.45 KDa
Source organism: Spinacia oleracea
Expression system: Escherichia coli
UniProt:
  • Canonical: P00289 (Residues: 70-168; Coverage: 59%)
Gene name: PETE
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

2 bound ligands:
Ligand CU 2 x CU
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: P3221
Unit cell:
a: 52.32Å b: 52.32Å c: 127.36Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.159 0.157 0.204
Expression system: Escherichia coli

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